检索结果-南通市图书馆

Testis-specific Fankl gene in knockdown mice produces oligospermia via apoptosis

Asian Journal of Andrology 2014年第1期16卷 124-130页

作者： wan-Wei dong Hua-Liang Huang Wei Yang Jia Liu Yang Yu Sheng-Lai Zhou Wei wang Xiang-Chuan Lv Zhao-Yang LP Mei-Ying Zhang Zhi-Hong Zheng Wei Yan Laboratory Animal Centre China Medical University Shenyang China Department of Clinical Laboratory The Third Affiliated Hospital of Inner Mongonia MedicalUniversity Baogang Hospital Baotou China Department of Pathology and Pathophysiology Research China Medical University Shenyang China and Department ofPhysiology and Cell Biology University of Nevada School of Medicine Reno Nevada USA.

Fankl is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis. A reduction in sperm number and an increase in apoptotic spermatocytes were observed in Fankl-knockdown mice, and the apoptotic cells were found to be primarily spermatogonia and spermatocytes. The CAST results demonstrated that the consensus DNA-binding sequence was AAAAAG, in which the percentage occurrence of each base at each position ranged from 55 to 86%. This sequence was present in the promoter regions of 10 differentially expressed genes that were examined using microarray analysis. In total, 17 genes were differentially expressed with changes in their expression levels greater than twofold. The abnormal expression of Fankl target genes that were regulated directly or indirectly by Fankl reduced the number of sperm in the knockdown mice. Thus, FANK1 may Dlav a pivotal role in sDermato^enesis as a transcription *** is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis, A reduction in sperm number an

关键词： Fankl knockdown mice spermatogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diagnostic performance of the current risk-stratified approach with computed tomography for suspected choledocholithiasis and its options when negative finding

引用

Hepatobiliary & Pancreatic Diseases International 2019年第4期18卷 366-372页

作者： Hyun Woo Lee Tae Jun Song Do Hyun Park Sang Soo Lee dong-wan Seo Sung Koo Lee Myung-Hwan Kim Jae Hyuck Jun Ji Eun Moon Yeon Han Song Digestive Disease Center and Research Institute Department of Internal MedicineSoonchunhyang University School of MedicineBucheonKorea Division of Gastroenterology Department of Internal MedicineUniversity of Ulsan College of MedicineAsan Medical CenterSeoulKorea Division of Gastroenterology Department of Internal MedicineUniversity of Eulji College of MedicineEulji Medical CenterDaejeonKorea Department of Biostatistics Clinical trial centerSoonchunhyang University Bucheon HospitalBucheonKorea

Background:Several studies evaluated the current guideline of the American Society for Gastrointestinal Endoscopy(ASGE)and reported only suboptimal *** study evaluated the diagnostic performance of the ASGE guideline based on computed tomography(CT)and role of endoscopic ultrasonography(EUS)and magnetic resonance cholangiopancreatography(MRCP)in patients with suspected choledocholithiasis but negative CT ***:Patients with suspected choledocholithiasis undergoing ERCP between January 2016 and January 2017 were retrospectively *** patients underwent CT to detect *** or MRCP was performed when the CT scan showed negative *** were classified into the high and intermediate-risk groups,based on predictors from the ASGE ***:Of 583 patients with suspected choledocholithiasis,340(58.3%)had stones on ERCP(65.9%in the high-risk group and 40.6%in the intermediate-risk group).The accuracy of ASGE guideline for CT was 63.98%(79.12%sensitivity,42.80%specificity)and 36.02%(20.88%sensitivity,57.20%specificity)in the high-risk and intermediate-risk groups,*** 103 patients in the high-risk group underwent both CT and US,the accuracy of CT was higher than that of US for detecting choledocholithiasis(78.64%vs.53.40%),with a significant difference in area under the curve(AUC)(0.78 vs.0.59,P<0.001).Of 339 with negative CT finding,the accuracy of EUS was higher than that of MRCP(90.91%vs.82.76%),but with no significant difference in AUC(0.91 vs.0.83,P=0.347).Conclusions:CT-based ASGE guideline showed superior diagnostic performance than US for predicting *** diagnostic options,EUS or MRCP,with negative CT finding showed comparable ***,the diagnostic modality should be selected based on availability,experience,cost,and contraindications.

关键词： Choledocholithiasis ASGE guideline Accuracy Endoscopic ultrasound Magnetic resonance cholangiopancreatography

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

面向Web论坛的网络信息获取技术及系统实现

引用

计算机工程与科学 2011年第1期33卷 157-160页

作者：彭冬蔡皖东西北工业大学计算机学院陕西西安710072

网络爬虫技术是网络信息获取的重要手段,面向Web论坛的信息获取则是网络爬虫技术所面临的新课题。在分析和研究面向Web论坛信息获取技术的基础上,本文设计和实现了一种用于Web论坛信息获取的主题网络爬虫系统,根据Web论坛信息组织结构,... 详细信息

网络爬虫技术是网络信息获取的重要手段,面向Web论坛的信息获取则是网络爬虫技术所面临的新课题。在分析和研究面向Web论坛信息获取技术的基础上,本文设计和实现了一种用于Web论坛信息获取的主题网络爬虫系统,根据Web论坛信息组织结构,提出了基于遍历策略的信息搜索技术;根据正文信息分布及论坛自身特点,提出了基于DOM与分块算法相结合的正文提取技术。实验结果表明,遍历策略比传统的网络爬虫遍历策略具有更高的效率,能够采集到更多主题相关度高的网页;经过噪声清洗处理后,有效提取网页正文,提高了信息采集精度。

关键词：网络爬虫 Web论坛正文提取主题相关度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A pilot proof-of-concept study of a modified device for one-step endoscopic ultrasound-guided biliary drainage in a new experimental biliary dilatation animal model

引用

World Journal of Gastroenterology 2014年第19期20卷 5859-5866页

作者： Tae Hoon Lee Jun Hyuck Choi Sang Soo Lee Hyun Deuk Cho dong wan Seo Sang-Heum Park Sung Koo Lee Myung-Hwan Kim Do Hyun Park Division of Gastroenterology Department of Internal MedicineSoonchunhyang University School of MedicineCheonan HospitalChungcheongnamdo 330-721South Korea Division of Gastroenterology Department of Internal MedicineUniversity of Ulsan College of MedicineSeoul 138-736South Korea Department of Pathology Soonchunhyang University School of MedicineCheonan HospitalChungcheongnam-do 330-721South Korea

AIM: To evaluate the technical feasibility of a modified tapered metal tip and low profile introducer for one-step endoscopic ultrasound (EUS)-guided biliary drainage (EUS-BD) in a new experimental biliary dilatation ... 详细信息

关键词： Endoscopic ultrasound Biliary drainage Biliary dilation Feasibility Complications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Simplified fistula dilation technique and modified stent deployment maneuver for EUS-guided hepaticogastrostomy

引用

World Journal of Gastroenterology 2014年第17期20卷 5051-5059页

作者： Woo Hyun Paik Do Hyun Park Jun-Ho Choi Joon Hyuk Choi Sang Soo Lee dong wan Seo Sung Koo Lee Myung-Hwan Kim Jung Bok Lee Division of Gastroenterology Department of Internal MedicineUniversity of Ulsan College of MedicineAsan Medical CenterSeoul 138-736South Korea Department of Internal Medicine Inje University Ilsan Paik HospitalKoyang 411-706South Korea Department of Clinical Epidemiology and Biostatics University of Ulsan College of MedicineAsan Medical CenterSeoul 138-736South Korea

AIM: To evaluate the success rates, procedural time and adverse event rates of the modified methods in endoscopic ultrasonography-guided hepaticogastrostomy (EUS-HGS).

关键词： Endoscopic ultrasonography Biliary drainage Hepaticogastrostomy Treatment outcome Adverse event

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Customized lipid-coated magnetic mesoporous silica nanoparticle doped with ceria nanoparticles for theragnosis of intracerebral hemorrhage

引用

Nano Research 2018年第7期11卷 3582-3592页

作者： Bong Geun Cha Han-Gil Jeong dong-wan Kang Myong-Joo Nam Chi Kyung Kim Do Yeon Kim In-Young Choi Seul Ki Ki Song I Kim Ju hee Han Jaeyun Kim Seung-Hoon Lee School of Chemical Engineering Sungkyunkwan University Suwon 16419 Republic of Korea Department of Laboratory of Innovative Nanobiotechnology Biomedical Research Institute Seoul National University Hospital 101 Daehak-ra Jongno-gu Seou103080 Republic of Korea Department of Neurology Seoul National University Hospital 101 Daehak-ro Jongno-gu Seou103080 Republic of Korea Department of Neurology Korea University Guro Hospital and Korea University College of Medicine Seoul 08308 Republic of Korea Department of Health Sdences and Technology Samsung Advanced Institute for Health Science & Technology (SAIHST) Sungkyunkwan University Suwon 16419 Republic of Korea Biomedical Institute for Convergence at SKKU (BICS) Sungkyunkwan University Suwon 16419 Republic of Korea

Intracerebral hemorrhage （ICH）, caused by the sudden rupture of an artery within the brain, is a devastating subtype of stroke, which currently has no effective treatment. Intense inflammatory reactions that occur in the peri-hematomal area after ICH are more deleterious than the hematoma itself, resulting in subsequent brain edema and neurologic deterioration. Thus, we developed lipid-coated magnetic mesoporous silica nanoparticles doped with ceria nanoparticles （CeNPs）, abbreviated as LMCs, which have both potent anti-inflammatory therapeutic effects via scavenging reactive oxygen species and help in increasing the efficacy of magnetic resonance imaging enhancement in the peri-hematomal area. LMCs consist of mesoporous silica nanopartide-supported lipid bilayers, which are loaded with large amounts of CeNPs for scavenging of reactive oxygen species, and iron oxide nanoparticles for magnetic resonance imaging contrast. LMCs loaded with CeNPs exhibited strong anti-oxidative and anti-inflammatory activities in vitro. In the rodent ICH model, intracerebraUy injected LMCs reached the peri-hematomal area and were engulfed by macrophages, which were clearly visualized by magnetic resonance imaging of the brain. Moreover, LMCs reduced inflammatory macrophage infiltration, and thus significantly reduced brain edema. Finally, LMC treatment markedly improved neurologic outcomes of the animals with ICH. Thus, LMC is the first nanobiomaterial that successfully showed theragnostic effects in ICH.

关键词： magnetic mesoporous silica nanoparticle ceria nanoparticles intracerebral hemorrhage reactive oxygen species therapeutics imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

混合碳四全加氢镍系催化剂的制备与性能评价

引用

石化技术与应用 2024年第5期42卷 319-323页

作者：郝昭陈明林刘军董万军展学成吕龙刚马好文中国石油四川石化有限责任公司化工一部四川成都611930 中国石油石油化工研究院兰州化工研究中心甘肃兰州730060

使用共沉淀法和挤条成型工艺小试制备了混合碳四全加氢镍系催化剂,考察了反应压力、体积空速、氢烃体积比等加氢工艺条件对催化剂性能的影响;采用打片成型工艺进行催化剂中试放大制备,并对其性能进行了评价。结果表明:在入口温度为室温... 详细信息

使用共沉淀法和挤条成型工艺小试制备了混合碳四全加氢镍系催化剂,考察了反应压力、体积空速、氢烃体积比等加氢工艺条件对催化剂性能的影响;采用打片成型工艺进行催化剂中试放大制备,并对其性能进行了评价。结果表明:在入口温度为室温,反应压力为2.0 MPa,氢烃体积比为300,进料体积空速为2.0 h^(-1)的优化加氢工艺条件下,小试催化剂可以将混合碳四原料中单烯烃质量分数降低至1.0%以下;与小试催化剂相比,中试放大催化剂堆积密度和径向抗压碎力分别提高73%,302%;中试放大催化剂与进口钯系催化剂在789 h评价周期内的不饱和烃转化率分别为94.26%,89.72%,前者比后者具有更优的低温加氢活性和加氢稳定性。

关键词：混合碳四全加氢中试放大镍系催化剂钯系催化剂转化率

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

当代生态社会主义的局限与马克思生态学的重构

引用

内蒙古社会科学 2017年第5期38卷 17-23页

作者：万冬冬中共陕西省委党校科社教研部陕西西安710061

针对当代生态社会主义的局限,美国生态马克思主义理论家伯克特从政治经济学的角度重构了马克思生态学的方法、维度和指向。伯克特认为马克思生态学的方法符合社会生态学的四个条件:物质规范和社会规范相结合、相关整体论、定性分析和定... 详细信息

针对当代生态社会主义的局限,美国生态马克思主义理论家伯克特从政治经济学的角度重构了马克思生态学的方法、维度和指向。伯克特认为马克思生态学的方法符合社会生态学的四个条件:物质规范和社会规范相结合、相关整体论、定性分析和定量分析相结合以及教育和实践相结合。伯克特从马克思自然观所具有的生态意蕴的维度重构了马克思生态学,并把实现人与自然的协同进化作为其重构的指向。伯克特对马克思生态学的重构,不仅深化了对马克思理论本身的认识,而且拓展了生态马克思主义研究的视域。

关键词：伯克特马克思生态学社会生态学自然观协同进化

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

金沙江上游堰塞湖相静水沉积物特性及其资源化利用研究

引用

环境工程 2024年第8期42卷 1-7页

作者：权蔚慈杨凯阚思蒙孙叶龙田应辉万东郑蕾程红光北京师范大学水科学研究院北京100875 华电金沙江上游水电开发有限公司成都610041

为解决水电站建设过程中疏浚大量沉积物的处置难题,在金沙江上游拉哇水电站坝址区采集了河床覆盖层中堰塞湖相沉积物样品,分析样品理化性质和重金属含量,评价沉积物中重金属污染程度及潜在生态风险,探讨沉积物资源化利用的可行途径。结... 详细信息

为解决水电站建设过程中疏浚大量沉积物的处置难题,在金沙江上游拉哇水电站坝址区采集了河床覆盖层中堰塞湖相沉积物样品,分析样品理化性质和重金属含量,评价沉积物中重金属污染程度及潜在生态风险,探讨沉积物资源化利用的可行途径。结果表明,沉积物样品pH值为7.77~7.91,呈弱碱性;沉积物样品不均匀系数Cμ>10,粒组较多,级配连续;沉积物样品质地为壤土,包括砂质壤土和粉砂质壤土;沉积物有机质含量为1.33%~1.86%,属偏低等级。地累积指数法(Igeo)评价结果表明,沉积物样品未受重金属污染。潜在生态风险指数法评价结果表明,仅有Ⅰ号勘探线沉积物样品中的Cd存在中等潜在生态风险。综上,拉哇水电站坝址堰塞湖相静水沉积物可作为替代表土的覆盖物用于园林绿化或建筑材料。但由于沉积物自身有机质匮乏,可以通过添加当地市政污泥、餐厨垃圾、畜禽粪便及其堆肥产品等富含有机质和营养元素的废弃物来提高沉积物覆盖层肥力。此外,还可以通过掺混矿煤渣、水泥等,利用固化技术提高沉积物基建材的抗压和抗折强度。

关键词：金沙江上游堰塞湖相静水沉积物土地利用建筑材料

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mesh结构无线传感器网络中的自适应跳频机制

引用

北京邮电大学学报 2011年第5期34卷 86-91页

作者：赵金东王沁万亚东北京科技大学信息工程学院北京100086 烟台大学计算机学院山东烟台264005

提出了一种用于Mesh拓扑结构无线传感器网络的自适应跳频算法,网络中的节点根据邻居的连接信息选择自己与邻居通信的备用信道,一旦当前信道质量变差,通信双方放弃当前信道,使用备用信道进行通信,从而提高了网络的可靠性.算法有效地利用... 详细信息

提出了一种用于Mesh拓扑结构无线传感器网络的自适应跳频算法,网络中的节点根据邻居的连接信息选择自己与邻居通信的备用信道,一旦当前信道质量变差,通信双方放弃当前信道,使用备用信道进行通信,从而提高了网络的可靠性.算法有效地利用了多信道进行通信,在网络密度不太大且网络拓扑变化不频繁的情况下是有效的.

关键词：无线传感器网络多信道通信自适应跳频 IEEE802.15.4 无线Mesh网络

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：