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Development of a multiplex polymerase chain reaction assay for detection of hepatitis C virus,hepatitis B virus,and human immunodeficiency virus 1

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World Journal of Virology 2024年第1期13卷 95-106页

作者： waleed abdelgaber nemr Radwan K Nashwa Department of Radiation Microbiology National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairo 11371Egypt Department of Health Radiation Research National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairo 11371Egypt

BACKGROUND Hepatitis C virus(HCV),hepatitis B virus(HBV),and human immunodeficiency virus 1(HIV-1)are the most epidemic blood-borne viruses,posing threats to human health and causing economic losses to nations for combating the infection *** diagnostic methodologies that depend on the detection of viral nucleic acids are much more expensive,but they are more accurate than sero-logical *** To develop a rapid,cost-effective,and accurate diagnostic multiplex polymerase chain reaction(PCR)assay for simultaneous detection of HCV,HBV,and *** The design of the proposed PCR assay targets the amplification of a short conserved region featured with a distinguishable melting profile and electro-phoretic molecular weight inside each viral ***,this diagnostic method will be appropriate for application in both conventional(combined with electrophoresis)and real-time PCR *** in silico investigations were conducted to prove the capability of the approached PCR assay to detect variants of each ***,Egyptian isolates of each virus were subjected to the wet lab examination using the given diagnostic *** The in silico investigations confirmed that the PCR primers can match many viral variants in a multiplex PCR *** wet lab experiment proved the efficiency of the assay in distinguishing each viral type through high-resolution melting *** to related published assays,the proposed assay in the current study is more sensitive and competitive with many expensive PCR *** This study provides a simple,cost-effective,and sensitive diagnostic PCR assay facilitating the detection of the most epidemic blood-borne viruses;this makes the proposed assay promising to be substitutive for the mistakable and cheap serological-based assays.

关键词： Diagnosis Blood-borne viruses Multiplex polymerase chain reaction High-resolution melting

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