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Antimicrobial activity of latex silver nanoparticles using Calotropis procera

Asian Pacific Journal of Tropical Biomedicine 2014年第11期4卷 876-883页

作者： Nadia Hussein Mohamed Mady Ahmed Ismail wael moustfa abdel-mageed Ahmed abdelfattah Mohamed Shoreit Department of Botany Faculty of ScienceAssiut University Biology Department.Faculty of Science and Art SamtahJazan University Pharmacognosy Department College of PharmacyKing Saud University Pharmacognosy Department Faculty of PharmacyAssiut University

Objective:To synthesize silver nanoparticles(AgNPs) by green methods using scrum latex of Calotropis procera at 80 ℃ and evaluate them against bacteria,dermatophytes and phytopathogenic fungi comparing with the activity of untreated ***:The synthesis of AgNPs was performed by mixing 3%latex scrum extract with the same volume of silver nitrate(2 mmol/L) solution in round flask and heating in water bath at80 ℃.Characterization of silver particles were determined using UV-vis spectrophotometer,transmission electron microscopy(TEM),X-ray diffraction and Fourier transform infrared *** antimicrobial activity of the green synthesized AgNPs was determined against bacteria,dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion ***:Biosynthesis of latex silver nanoparticles was successfully obtained by green *** formation of AgNPs has been confirmed by UV-vis,TEM microscopy.X-ray diffraction and Fourier transform infrared *** analysis showed that synthesized AgNPs are highly stable spherical shaped particles,well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 *** showed strong antibacterial activity against Gram-negative bacteria(Escherichia coli,Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum,Candida albicans and Aspergillus ***:It can be concluded that serum latex of Calotropis pmcera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions(Ag^+ to Ag^0).The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex.

关键词： Green synthesis Calotropis procera Latex Silver nanoparticles Antimicrobial activity

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