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BMPRⅡ^(+)neural precursor cells isolated and characterized from organotypic neurospheres:an in vitro model of human fetal spinal cord development

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Neural Regeneration Research 2024年第2期19卷 447-457页

作者： Michael w.w.ible ii Michael D.Lovelace Hamish D.Mundell Tsz w.i Rosita Pang Tailoi Chan-Ling Bosch institute Discipline of Anatomy and Histology(F13)University of SydneySydneyNSWAustralia School of Environment and Science Griffith UniversityNathanQLDAustralia Discipline of Medicine Nepean Clinical SchoolFaculty of Medicine and HealthUniversity of SydneyKingswoodNSWAustralia New South wales Brain Tissue Resource Centre School of Medical SciencesFaculty of Medicine and HealthUniversity of SydneyCharles Perkins Centre(D17)SydneyNSWAustralia

Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not well ***-ⅡBMP receptor(BMPRⅡ),the cognate receptor,is expressed by neural precursor cells during embryogenesis;however,an in vitro method of enriching BMPRⅡ^(+)human neural precursor cells(hNPCs)from the fetal spinal cord is *** was undertaken on intact second-trimester human fetal spinal cord using antibodies to BMPRⅡand leukemia inhibitory factor(LiF).Regions of highest BMPRⅡ^(+)immunofluorescence localized to sensory *** and meningeal-associated BMPRⅡ^(+)vascular cells were identified in both intact fetal spinal cord and cortex by co-positivity with vascular lineage markers,CD34/*** immunostaining identified a population of somas concentrated in dorsal and ventral horn interneurons,mirroring the expression of LiF receptor/CD118.A combination of LiF supplementation and high-density culture maintained culture growth beyond 10 passages,w.ile synergistically increasing the proportion of neurospheres with a stratified,*** neurospheres were characterized by BMPRⅡ^(+)/MAP2ab^(+/–)/βⅢ-tubulin^(+)/nestin^(–)/vimentin^(–)/GFAP^(–)/NeuN^(–)surface hNPCs surrounding a heterogeneous core ofβⅢ-tubulin^(+)/nestin^(+)/vimentin^(+)/GFAP^(+)/MAP2ab^(–)/NeuN^(–)multipotent *** cultures from tripotential neurospheres contained neuronal(βⅢ-tubulin^(+)),astrocytic(GFAP+),and oligodendrocytic(O4+)lineage ***-activated cell sorting-sorted BMPRⅡ^(+)hNPCs were MAP2ab^(+/–)/βⅢ-tubulin^(+)/GFAP^(–)/O4^(–)in *** is the first isolation of BMPRⅡ^(+)hNPCs identified and characterized in human fetal spinal *** data show that LiF combines synergistically with high-density reaggregate cultures to support the organotypic reorganization of neurospheres,characterize

关键词： BMPRⅡ bone morphogenetic protein histotypic human spinal cord development leukemia inhibitory factor neurosphere organotypic reaggregate sensory columns

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in situ digital testing method for quality assessment of soft soil improvement with polyurethane

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1732-1748页

作者： X.F.w.ng C.J.w.ng w.V.Yue Z.J.Zhang Z.Q.Yue Department of Civil Engineering China University of Geosciences (Beijing)BeijingChina Department of water Science and Engineering Zhengzhou UniversityZhengzhouChina Department of Geotechnical Engineering Tongji UniversityShanghaiChina Department of Civil Engineering The University of Hong KongChina

This study purposes an in situ testing method on quality assessment of soil *** drilling data includes the spatial distribution and in situ strength of untreated and treated soil along three different drillholes measured by on-site drilling monitoring *** factual drilling data can characterize the degree of soil improvement by penetration injection with permeable *** from on-site drilling monitoring shows that the linear zones represent constant drilling speeds shown in the plot of drill bit advancement *** drilling time,w.ich indicates the spatial distributions of soil *** soil profile at the study site is composed of four layers,w.ich includes fill,untreated silty clay,treated silty clay,and mucky *** results of soil profile are verified by the parallel site *** constant drilling speeds profile the coring-resistant strength of drilled *** comparing with the untreated silty clay,the constant drilling speeds of the treated silty clay have been decreased by 13.0-62.8%.Two drilling-speed-based indices of 61.2%and 65.6%are proposed to assess the decreased average drilling speed and the increased in situ strength of treated silty *** tests,*** compressive strength(UCS)test,have been performed with core sample to investigate and characterize in situ strength by comparing that with drilling *** show that the average predicted strengths of treated silty clay are 2.4-6.9 times higher than the average measured strength of untreated silty *** UCS-based indices of 374.5%and 344.2%verified the quality assessment(QA)results by this new.in situ *** method provides a cost-effective tool for quality assessment of soil improvement by utilizing the digital drilling data.

关键词： Drilling process monitoring system Hydraulic rotary drilling process Constant drilling speed Soil improvement Quality assessment

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Electrolyte Effect on Photoetching of Gallium Nitride

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Nanomanufacturing and Metrology 2024年第1期7卷 61-74页

作者： Huiqiang Liang Zhenghao w.i Jiongchong Fang Yanming Li Changli Li Zhirun Xie Yun Hau ng Guosong Zeng Shenzhen Key Laboratory of intelligent Robotics and Flexible Manufacturing Systems Southern University of Science and TechnologyShenzhen 518055People’s Republic of China Department of Mechanical and Energy Engineering Southern University of Science and TechnologyShenzhen 518055People’s Republic of China School of Materials Sun Yat-Sen UniversityGuangzhou 510275People’s Republic of China School of Energy and Environment City University of Hong KongKowloon 999077Hong Kong SARPeople’s Republic of China City University of Hong Kong Shenzhen Research institute Shenzhen Hi-Tech Industrial ParkNanshan DistrictShenzhen 518000People’s Republic of China SUSTech Energy institute for Carbon Neutrality Southern University of Science and TechnologyShenzhen 518055People’s Republic of China

The limited material removal rate of conventional chemical mechanical polishing(CMP)significantly hinders the fabrica-tion efficiency and surface quality,thereby preventing the development of gallium nitride(GaN)-based ***,the incorporation of photoelectrochemistry in CMP has garnered increasing attention because of its potential to enhance the quality and efficiency of the GaN ***,a considerable gap still exists in the comprehensive understanding of the specific photoelectrochemical(PEC)behavior of ***,we report the influence of the electrolyte on the PEC etching of *** acids and bases were tested,with their pH being carefully *** concentrations of the cations and anions were also *** results showed that photocorrosion/photoetching was more pronounced in sulfuric acid,phosphoric acid,and nitric acid environments than in alkaline environments,but it was less pronounced in hydrochloric ***,the effects of pH and anion concentration on photoetching were investigated,and the results revealed that pho-toetching in acidic environments weakened with increasing pH levels and diminished with increasing sulfate *** underlying reasons contributing to this observation were *** findings provide ideas for improving the pho-toetching efficiency of GaN,thereby enriching the photoelectrochemical mechanical polishing(PECMP)technology of GaN.

关键词： GaN Photoelectrochemistry Photoetching pH

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Mitochondrial therapeutics and mitochondrial transfer for neurodegenerative diseases and aging

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Neural Regeneration Research 2025年第3期20卷 794-796页

作者： Neville ng Michelle Newbery Nicole Miles Lezanne Ooi Molecular Horizons and School of Chemistry and Molecular Bioscience University of WollongongWollongongNSWAustralia

Mitochondrial dysfunction and neurodegeneration:Progressive neurodegenerative diseases affect a significant proportion of the population;in a single year,there are as many as 276 million disabilities and 9 million dea... 详细信息

关键词： degenerative diseases Mitochondrial

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Precipitation of multi-type nano-quasicrystals in a Mg-Zn-Y alloy

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Journal of Magnesium and Alloys 2024年第3期12卷 997-1008页

作者： w.Z.w.ng X.Z.Zhou L.F.Zhang H.Q.Ye Z.Q.Yang Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of ScienceShenyang 110016China Ji Hua Laboratory Foshan 528251China Foshan University Foshan 528231China

Mg-6Zn-1Y(at.%)ribbons with strengthening precipitates of multi-type nanoquasicrystals were prepared by melt-spinning followed by aging *** evolution of the rapidly solidified ribbons during isothermal aging was comprehensively studied using various electron microscopy *** new kinds of decagonal quasicrystals were formed in aged ribbons,in addition to precipitation of nanometer icosahedral ***-resolution observations reveal that both decagonal quasicrystals can be modeled by quasiperiodic tiling with decagonal clusters of 2.5 nm in diameter,but overlap of neighboring clusters in both decagonal quasicrystals is different from the Gummelt model observed in other quasicrystals.A shell composed of complex Laves Mg-Zn domains was formed surrounding each decagonal quasicrystal precipitate upon prolonged *** addition,all kinds of nanoprecipitates exhibit excellent structure and size stability at 573 *** findings may have implications for not only fundamental studies about quasicrystals,but also microstructural manipulation of high-performance Mg alloys.

关键词： Quasicrystal Mg alloys Rapid solidification Scanning-transmission electron microscopy.

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Topology modulation of tetraphenylethylene graphdiyne at a liquid-liquid interface

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Nano Research 2024年第6期17卷 4661-4667页

作者： Tingting Xu Pei wen ng Shaofei wu danjun Fu Yunhan Ma Yao Liu Lidao Li Xiaofei Zhang Jun Zhu Department of Chemistry National University of SingaporeSingapore 117543Singapore institute of Materials Research and Engineering(iMRE) Agency for ScienceTechnology and Research(A*STAR)Singapore 138634Singapore

Monomers with low symmetries can form different topological structures in the preparation of organic two-dimensional(2D)***,it remains challenging to modulate the topologies in practical *** theoretical insights into the formation energy of potential structural configurations,we report the topology modulation of a graphdiyne(GDY)derivative constructed from two-fold symmetric tetrakis(4-ethynylphenyl)ethene precursor by changing solvent combinations in a liquid-liquid interfacial *** aqueous-organic(water-dichloromethane)interface afforded GDY with a kagome topology w.ile a rhombic topology was formed at an organic-organic(hexane-acetonitrile)interface.A comprehensive evaluation of their structures and optoelectronic properties was conducted through various characterization techniques and theoretical *** study provided new.insights to modulate the topology of not only GDY but also other framework structures and obtain topologically pure materials in situations where different topologies are possible during practical synthesis.

关键词： graphdiyne derivative tetraphenylethylene topology modulation aggregation-induced emission(AiE)two-dimensional(2D)polymers interfacial synthesis

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inflow and outflow permeability tests in a very soft clay under low stresses

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第8期16卷 3270-3283页

作者： J.w.S.Vargas F.A.B.danziger F.R.Lopes T.Lunne Federal University of Rio de Janeiro Rio de JaneiroBrazil Norwegian Geotechnical institute OsloNorway

in situ inflow and outflow permeability tests with the BAT probe at Sarapuíii soft clay test site are presented.A description of the BAT permeability test is provided,discussing its advantages and shortcomings,especially in the case of very soft clays under low *** pressures were monitored during probe installation and were found to be slightly lower than piezocone u2 pore pressures,consistent with the position of the *** role of filter tip saturation was investigated after the usual saturation procedure provided an unsatisfactory pore pressure response during probe *** show that the vacuum saturation procedure provides adequate response during installation and increases the reliability of the coefficient of permeability determination in early *** inflow and outflow tests yielded similar results,indicating that careful execution of the test can lead to good test repeatability regardless of the loading *** sequences of alternated inflow and outflow tests have yielded similar results,indicating that soil reconsolidation and filter clogging were negligible in the tests *** are presented concerning the relationship between index parameters and the in situ coefficient of permeability for Sarapuíii clay,w.ich plot outside the range of existing databases.

关键词： Permeability test Soft clay BAT probe inflow and outflow tests

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Protein-activated atomic layer deposition for robust crude-oil-repellent hierarchical nano-armored membranes

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Science Bulletin 2024年第2期69卷 218-226页

作者： Xiaobin Yang Pan Sun Yajie wen Anil U.Mane Jeffrey w.Elam Jun Ma Shaomin Liu Seth B.darling Lu Shao MiiT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage State Key Laboratory of Urban Water Resource and EnvironmentSchool of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China Chemical Sciences and Engineering Division Argonne National LaboratoryLemont IL 60439USA Applied Materials Division Argonne National LaboratoryLemont IL 60439USA Advanced Materials for Energy-water Systems Energy Frontier Research Center Argonne National LaboratoryLemont IL 60439USA Pritzker School of Molecular Engineering University of ChicagoChicago IL 60637USA Department of Physics University of Ilinois at ChicagoChicago IL 60607USA School of Environment Harbin Institute of TechnologyHarbin 150090China wA School of Mines:Minerals Energy and Chemical EngineeringCurtin UniversityPerth WA 6845Australia

Atomic layer deposition(ALD)offers unique capabilities to fabricate atomically engineered porous materials with precise pore tuning and multi-functionalization for diverse applications like advanced membrane separations towards sustainable energy-water ***,current ALD technique is inhibited on most non-polar polymeric membranes due to lack of accessible nucleation ***,we report a facile method to efficiently promote ALD coating on hydrophobic surface of polymeric membranes via novel protein activation/*** a proof of concept,TiO2 ALD-coated membranes activated by bovine serum albumin exhibit remarkable superhydrophilicity,ultralow underwater crude oil adhesion,and robust tolerance to rigorous environments including acid,alkali,saline,and *** importantly,excellent cyclable crude oil-in-water emulsion separation performance can be *** mechanism for activation/sensitization is rooted in reactivity for a particular set of amino ***,the universality of protein-sensitized ALD is demonstrated using common egg w.ite,promising numerous potential usages in biomedical engineering,environmental remediation,lowcarbon manufacturing,catalysis,and beyond.

关键词： Membrane separation Atomic layer deposition water treatment Antifouling

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Superdirective Circular Arrays of Electric and Huygens Dipole Elements

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Electromagnetic Science 2024年第1期2卷 38-62页

作者： Richard w.Ziokowski TheUniversityofArizona TucsonAZ85721USA

Uniform circular arrays(UCAs)provide both omnidirectional(360°)and directive(sector)coverage of the azimuthal *** versions with unidirectional,high front-to-back ratio(FTBR)properties could provide the radiated field char-acteristics being pursued for NextG wireless networks and their perceived *** UCA configurations–full,semi-circular,and sector–that radiate vertically-polarized(VP)fields and are composed of either omnidirectional electric dipole ele-ments or unidirectional Huygens dipole elements are analyzed first with conventional methods as reference *** omni-and uni-directional element configurations are then treated with several optimization techniques:the classic Rayleigh-quotient(RQ)method and its unidirectional-constrained version;the eigenbeam decomposition and synthesis(EBDS)technique used to design su-perdirective acoustic receiving arrays;and the Bessel-azimuthal multipole(BEAM)approach developed *** arrays are identified as being superdirective with extremely high FTBR *** performance characteristics of the arrays of unidirectional elements are demonstrated to be superior in ***,it is shown that larger radius arrays with RQ-specified excitation amplitudes are robust to changes in them whereas the outcomes of the corresponding small radius versions are *** the other hand,the BEAM-optimized densely-packed small-radius superdirective arrays are quite tolerant to those variations w.ile generating unidirectional pseudo-needle beams.

关键词： Directivity Huygens dipole antennas Multipoles Needle radiation Optimization Superdirective arrays Uniform circular arrays.

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Cavity-agnostic acoustofluidic manipulations enabled by guided flexural waves on a membrane acoustic waveguide actuator

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Microsystems & Nanoengineering 2024年第2期10卷 153-171页

作者： Philippe Vachon Srinivas Merugu Jaibir Sharma Amit Lal Eldwin J.ng Yul Koh Joshua E.-Y.Lee Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingaporeSingapore institute of Microelectronics Agency for ScienceTechnology and Research(A*STAR)SingaporeSingapore SonicMEMS Laboratory School of Electrical and Computer EngineeringCornell UniversityIthacaNYUSA School of Electrical and data Engineering University of Technology SydneyUltimoNSWAustralia

This article presents an in-depth exploration of the acoustofluidic capabilities of guided flexural waves(GFws)generated by a membrane acoustic waveguide actuator(MAwA).By harnessing the potential of GFws,cavity-agnostic advanced particle manipulation functions are achieved,unlocking new avenues for microfluidic systems and lab-on-a-chip *** localized acoustofluidic effects of GFws arising from the evanescent nature of the acoustic fields they induce inside a liquid medium are numerically investigated to highlight their unique and promising *** traditional acoustofluidic technologies,the GFws propagating on the MAwA’s membrane waveguide allow for cavity-agnostic particle manipulation,irrespective of the resonant properties of the fluidic ***,the acoustofluidic functions enabled by the device depend on the flexural mode populating the active region of the membrane *** demonstrations using two types of particles include in-sessile-droplet particle transport,mixing,and spatial separation based on particle diameter,along with streaming-induced counter-flow virtual channel generation in microfluidic PDMS *** experiments emphasize the versatility and potential applications of the MAwA as a microfluidic platform targeted at lab-on-a-chip development and showcase the MAwA’s compatibility with existing microfluidic systems.

关键词： waveguide fluid cavity

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