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Human chemerin induces eryptosis at concentrations exceeding circulating levels

BIOCELL 2024年第8期48卷 1197-1208页

作者： MARYNA TKACHENKO ANATOLII ONISHCHENKO LILIYA TRYFONYUK DMYTRO BUTOV KATERYNA KOT VIKTORIIA NOVIKOVA LIWEI FAN volodymyr prokopiuk YURII KOT ANTON TKACHENKO Department of Internal Medicine No.2 Clinical Immunology and AllergologyKharkiv National Medical UniversityKharkiv61022Ukraine Research Institute of Experimental and Clinical Medicine Kharkiv National Medical UniversityKharkiv61022Ukraine Institute of Health National University of Water and Environmental EngineeringRivne61022Ukraine Department of Infectious Diseases and Phthisiology Kharkiv National Medical UniversityKharkiv61022Ukraine Biochemistry Department V.N.Karazin Kharkiv National University Ministry of Education and Science of UkraineKharkiv61022Ukraine Department of Chemistry BiochemistryMicrobiology and Food HygieneState Biotechnological UniversityKharkiv61022Ukraine Clinical Research Innovation Lab Floor 26 Block D Qidi Technology Building 8Tsinghua Technology ParkBeijing100084China Department of Cryobiochemistry Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of UkraineKharkiv61015Ukraine

Introduction:Human chemerin is an adipokine that regulates chemotaxis,inflammation,and glucose *** addition,accumulating evidence suggests that chemerin promotes apoptosis,autophagy,and ***,there are no data on its impact on *** current study aimed to analyze the effects of human active Glu^(21)-Ser^(157) chemerin on eryptosis in *** and Methods:Human chemerin 0-2-10-50μg/mL was incubated for 24 h with human erythrocytes(hematocrit 0.4%)obtained from eight healthy *** cytometry-based determination of phospholipid scrambling,reactive oxygen species(ROS)production,and intracellular Ca^(2+)levels was *** supplement data on ROS and Ca^(2+)signaling in chemerin-mediated eryptosis,incubation in the presence or absence of antioxidants vitamin C and N-acetylcysteine and Ca^(2+)-binding agent EGTA was carried out,*** microscopy-based techniques were used to detect reactive nitrogen species(RNS)generation,involvement of caspase-3 and caspase-8,as well as the state of lipid order in cell membranes of erythrocytes exposed to human Glu^(21)-Ser^(157) ***:Our observations suggest that human Glu^(21)-Ser^(157) chemerin had no impact on eryptosis parameters at 2μg/***,chemerin stimulated phosphatidylserine externalization,ROS production,and Ca^(2+)accumulation at higher concentrations suggesting activation of ***^(2+)uptake turned out to be at least partly required for chemerin-mediated ***-mediated erythrotoxicity was additionally mediated by RNS,caspase-3,and ***,Glu^(21)-Ser^(157) chemerin promoted reduction in the liquid-ordered phase of cell membranes in ***:The present study first discloses that human chemerin can induce eryptosis via Ca^(2+)-dependent mechanisms at concentrations noticeably exceeding circulating ***,chemerin-induced eryptosis can hardly contribute to eryptosis-mediated anemia in disease

关键词： Adipokine Cell death Chemerin Oxidative stress Eryptosis

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