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The Effect of Different Diet on Saliva

Spectral Analysis review 2017年第4期5卷 37-48页

作者： vladim.ra Tomecková Kristína Krajcíková Slavomíra Dolinská vladim.r komanický Tomás Samuely Department of Medical and Clinical Biochemistry Faculty of MedicinePavol Jozef Safárik University in KosiceKosiceSlovak Republic Institute of Physics Faculty of SciencePavol Jozef safárik University in KosiceKosiceSlovak Republic

The aim of this scientific research was to study the influence of various diets (ketogenic, low carb, mixed) on teeth and saliva structure of healthy individuals. The health condition of teeth and the influence of diet (sweets, water intake) were investigated using the questionnaires which were statistically processed by descriptive and inductive statistics. Saliva was examined using synchronous fluorescent fingerprint (SFF) and atomic force microscopy (AFM). The statistical evaluation of answers of respondents using the questionnaires about intake of sweets in their diet showed significant influence of sugars on formation of tooth decay of diet. The intake of water (volume and frequency) did not show significant influence on formation of tooth decay. Endogenous fluorescence and surface structure of saliva differed in healthy subjects with different diet. The most organized surface structure showed low carb diet (without crystals). The least organized surface structure showed ketogenic diet with the big crystals (1 - 2 μm). Untraditional methods (SFF and AFM) enable the new, sensitive and quick analysis of the mixture of saliva. Saliva of individuals with low carb diet showed the most organized structure. Low content of sugar in diet is important possibility of the prevention before dental carries.

关键词： Low Carb Diet Mixed Diet Ketogenic Diet Saliva Synchronous Fluorescence Fingerprint Atomic Force Microscopy

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Monitoring of Heart Ischemia in Blood Serum

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Spectral Analysis review 2016年第2期4卷 11-22页

作者： vladim.ra Tomečková vladim.r komanický Mohammad Kakoush Kristína Krajčíková Gabriela Glinská Monika Široká Lýdia Pundová Tomáš Samuely Dominika Hložná Dmytro Lotnyk Department of Medical and Clinical Biochemistry Faculty of Medicine Pavol Jozef Safárik University in Kosice Slovak Republic Institute of Physics Faculty of Science Pavol Jozef Safárik University in Kosice Slovak Republic 1st Department of Internal Medicine Faculty of Medicine Pavol Jozef Safárik University in Kosice Slovak Republic

Our aim was to study the selected cases of the patients with ischemic heart disease and to analyze the structure of blood serum of patients in comparison with control serum of healthy subjects by methods: synchronous fluorescence fingerprint and atomic force microscopy that are still not used in clinical practice. Our results of fluorescence analysis showed that blood serum of all patients with ischemic heart disease decreased intensity of fluorescence in comparison with control blood serum. Endogenous fluorescence of synchronous fluorescence fingerprint of blood serum of patients with unstable angina pectoris state after non ST elevation myocardial infarction;angina pectoris and arterial hypertension 3 was similar, but atomic force microscopy revealed differences in the structure of blood serum of patients with the angina pectoris. Blood serum of patients with angina pectoris exhibited disappearance of fluorescence peak with maximum fluorescence and showed lower fluorescence intensity than control blood serum and blood serum of patients with arterial hypertension 2. Profiles of synchronous fluorescence fingerprint of blood serum of patients with arterial hypertension stage 2 showed formation of the new fluorescent peak with maximum fluorescence, similar shape of synchronous fluorescence fingerprint and higher fluorescence intensity than blood serum of healthy subjects. Blood serum sensitively revealed changes in the body by using untraditional novel techniques which enable the analysis of the mixture of blood serum and might be a new possibility in study of heart ischemia diseases.

关键词： Blood Serum Fluorescence Analysis Atomic Force Microscopy Ischemic Heart Disease

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