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Chinese Journal of Chemical Engineering 2019年第7期27卷 1690-1698页

作者： vlada B.veljkovic Ana v. velickovic Jelena M. Avramovic Olivera S. Stamenkovic University of Nis Faculty of TechnologyBulevar oslobodenja 124Leskovac 16000Serbia Research&Development Center "Alfatec" Bulevar Nikole Tesle 63/5Nis 18000Serbia

The performances of the response surface methodology(RSM)in connection with the Box–Behnken,face central composite or full factorial design(BBD,FCCD or FFD,respectively)were compared for the use in modeling of the NaOH-catalyzed sunflower oil *** influence of temperature,catalyst loading,and ethanol-to-oil molar ratio(EOMR)on fatty acid ethyl esters(FAEE)content was *** three multivariate strategies were efficient in the statistical modeling and optimization of the influential process variables but BBD and FCCD realization involved less number of experiments,generating smaller costs,requiring less work and consuming shorter time than the corresponding *** three designs resulted in the same optimal catalyst loading(1.25%of oil)and EOMR(12:1).The reduced two-factorinteraction(2 FI)models based on the BBD and FCCD defined a range of optimal reaction temperature(25℃–75℃)and 25℃,respectively while the same model based on the 33 FFD appointed 75℃.The predicted FAEE content of about 97%–98.0%was close to the experimentally obtained FAEE content of about 97.0%–97.6%under the optimal reaction ***,the simpler BBD or FCCD might successfully be applied for statistical modeling of biodiesel production processes instead of the more extensive,more laborious and more expensive FFD.

关键词： Biodiesel Box-Behnken design Model reduction Face central composite design Full factorial design Optimization

Optimization and kinetic modeling of waste lard methanolysis in a continuous reciprocating plate reactor

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Chinese Journal of Chemical Engineering 2019年第10期27卷 2481-2490页

作者： Marija R.Miladinovic Ivan J.Stojkovic Ana v.velickovic Olivera S.Stamenkovic Ivana B.Bankovic-Ilic vlada B.veljkovic University of Nis Faculty of TechnologyBulevar osloboaenja 124Leskovac 16000Serbia University of Belgrade Faculty of Technology and MetallurgyKarnegijeva 4Belgrade 11000Serbia Serbian Academy of Sciences and Arts(SASA) Knez Mihailova 35Belgrade 11000Serbia

Continuous biodiesel production from a waste pig-roasting lard,methanol and KOH was carried out in a reciprocating plate reactor(RPR)using a factorial design containing three process factors,namely methanol/lard molar ratio,catalyst loading,and normalized height of the *** main goals were to optimize the influential process factors with respect to biodiesel purity using the response surface methodology and to model the kinetics of the transesterification reaction in order to describe the change of triacylglycerols(TAG)and fatty acid methyl esters(FAME)concentrations along the RPR *** first-order rate law was proved for both the reaction and the mass *** model of the changing reaction mechanism and mass transfer of TAG was also *** kinetic models agreed with the experimental concentrations of TAG and FAME determined along the RPR height.

关键词： Biodiesel Kinetics Optimization Reciprocating plate reactor Waste lard

催化吲哚生成靛蓝的细胞色素P450BM-3定向进化研究

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生物化学与生物物理进展 2005年第7期32卷 630-635页

作者：李红梅梅乐和 URLACHER vlada SCHMID ROLF D 浙江大学化学工程与生物工程系杭州310027 Institute of Technical Biochemistry University of Stuttgart Stuttgart Germany

以催化吲哚产生的靛蓝在630nm处具有特殊的吸收峰为高通量筛选指标,将来源于Bacillusmegaterium的细胞色素P450BM-3单加氧酶的基因序列用易错聚合酶链式反应进行定向进化,通过多轮突变,在原有的能产靛蓝的高活力突变酶的基础上成功获得... 详细信息

以催化吲哚产生的靛蓝在630nm处具有特殊的吸收峰为高通量筛选指标,将来源于Bacillusmegaterium的细胞色素P450BM-3单加氧酶的基因序列用易错聚合酶链式反应进行定向进化,通过多轮突变,在原有的能产靛蓝的高活力突变酶的基础上成功获得了三个高于亲本酶的突变酶,突变酶的酶活分别是亲本酶的6.6倍(hml001),9.6倍(hml002)和5.3倍(hml003),并对突变酶的动力学参数进行了分析.突变酶DNA测序的结果表明,hml001含有一个有义氨基酸置换I39v,hml002含有三个有义氨基酸置换D168N,A225v,K440N,hml003含有一个有义氨基酸置换E435D,这些突变位点有些远离底物结合部位,有些位于底物结合部位.

关键词：细胞色素P450BM-3 定向进化靛蓝易错聚合酶链式反应催化活性

Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

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Chinese Journal of Chemical Engineering 2009年第3期17卷 478-483页

作者： Ivana Stanisavljevi Sаa Stojievi Dragan velikovi vlada veljkovi Miodrag Lazi Faculty of Technology University of Ni Chemical Industry Nevena A.D. Djordja Stamenkovia St. Zdravlje-Actavis Vlajkova 199

Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% etha-nol at a solid-to-liquid ratio of 1︰10 (m/v) and 25°C. The extract obtained by classical solvent extraction contained 29% larger amounts of phenolic compounds and 20% higher content of flavonoids. 2,2-diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 93.6% and the values of EC50 were (34.16±0.65) μg·ml-1 and (65.48±1.12) μg·ml-1 for the extracts obtained by the classical and ultrasound extractions, respectively. The extracts, independent of the extraction technique applied, showed a considerable growth inhibition on Candida albicans and Saccharo-myces cerevisiae, while no growth inhibition zones were observed for Aspergillus niger. The diameters of inhibition zone observed for all the microorganisms were larger for extracts obtained by classical extraction than those by ul-trasound extraction.

关键词： antioxidant activity antimicrobial activity Echinaceapurpurea L. total phenols flavonoids extraction

催化吲哚生成靛蓝的细胞色素P450BM-3定向进化研究

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催化吲哚生成靛蓝的细胞色素P450BM-3定向进化研究

以色列女性乳腺癌患者配偶健康相关生存质量低于健康女性的配偶

浙江省生物化学与分子生物学学术交流会

作者：李红梅梅乐和 vlada Urlacher Rolf D．Schmid 姚善泾浙江大学化学工程与生物工程系 Institute of Technical Biochemistry University of Stuttgart StuttgartGermany

以催化吲嗓产生的靛蓝在630nm处具有特殊的吸收峰为高通量筛选指标,将来源于 Bacillus megaterium的细胞色素P450BM-3单加氧酶的基因序列用易错聚合酶链式反应进行定向进化,通过多轮突变,在原有的能产靛蓝的高活力突变酶的基础上成功获... 详细信息

以催化吲嗓产生的靛蓝在630nm处具有特殊的吸收峰为高通量筛选指标,将来源于 Bacillus megaterium的细胞色素P450BM-3单加氧酶的基因序列用易错聚合酶链式反应进行定向进化,通过多轮突变,在原有的能产靛蓝的高活力突变酶的基础上成功获得了三个高于亲本酶的突变酶,突变酶的酶活分别是亲本酶的6．6倍(hml0001),9．6倍(hml002)和5．3(hml003)倍,并对突变酶的动力学参数进行了分析．突变酶DNA测序的结果表明hml001含有一个有义氨基酸王换139v,hml002含有三个有义氨基酸置换D168N,A225v,K440N,hml003含有一个有义氨基酸王换E435D,这些突变位点有些远离底物结合部位,有些位于底物结合部位的．

关键词： P450BM-3 定向进化靛蓝易错聚合酶链式反应催化活性

来源： cnki会议评论

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护士进修杂志 2017年第14期32卷 1257-1257页

作者：陈昌连王琦 Shor v Grinstein-Cohen O Reinshtein J

在以色列Recanati school for community health professions的vlada Shor等进行的一项研究中,比较了非转移性女性乳腺癌患者配偶和健康女性配偶之间的生存质量,并分析了两组研究对象心理一致性、社会人口学特征及患乳腺癌妻子的临床特... 详细信息

在以色列Recanati school for community health professions的vlada Shor等进行的一项研究中,比较了非转移性女性乳腺癌患者配偶和健康女性配偶之间的生存质量,并分析了两组研究对象心理一致性、社会人口学特征及患乳腺癌妻子的临床特征对其健康相关生存质量的影响。本研究最终纳入100名男性作为研究对象,其中观察组50名,为诊断为乳腺癌Ⅰ、Ⅱ、Ⅲ期并接受3~6个月化疗的女性乳腺癌患者的配偶;对照组50名,为社区健康女性的配偶。

关键词：女性乳腺癌生存质量社会人口学特征一致感观察组评估研究精神健康多元分析角色功能躯体疼痛