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A Study of Laser Effectiveness in Injured Tissues Regeneration

Journal of Biosciences and Medicines 2021年第3期9卷 55-63页

作者： Marcus Vinicius de Mello Pinto Aline Ronis Sampaio Miriam viviane Baron Carolina Macedo Alves Moreira Carolina Gonçalves Pinheiro Michele Paula dos santos Andres Felipe Mantilla Santamaria Cristine Brandenburg Nathália Ken pereira Iketani Mara Alessandra Porto dos Reis Oliveira Alexandre Sancho Felice Picariello Esteban Fortuny Juliano Tibola Nilton Petrone Vilardi Junior Celulare Institute Petrópolis Rio de Janeiro Brazil School of Medicine Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Brazil Dr. Daniel Thomaz Clinic Uberlandia Minas Gerais Brazil Faculty of Medicine of ABC (FMABC) Santo André Brazil Vale do Salgado Faculty (FVS) Ceará Brazil School of Medicine Industrial University of Santander (UIS) Bucaramanga Colombia Federal University of Ceará (UFC) Fortaleza Brazil Hospital Beneficiente Portuguesa of Belém Pará Brazil Clínica Instituto de Cirurgia Plástica Vênus Barreiras Bahia Brazil Unigranrio Rio de Janeiro Brazil da Universita Federico II di Napoli Italia e Pesquisador do Internacional Research Group Rio Grande do Norte Brazil Universidad Finis Terrae Santiago Chile UDESC Florianopolis SC Brazil Fluminense Football Club Rio de Janeiro RJ Brazil

This study aimed to understand the use of low level laser in the regeneration of injured tissues. Also to design our own opinion on the subject, as chemists. Laser is nothing more than a light with very special characteristics that grant it therapeutic properties. We can observe the existence of three different types of laser, being one of them the therapeutic laser, which is used in substitution for medications or along with them. Another type of laser is the surgical one and, in addition to these, there is a diagnostic type. A great advantage is that, instead of taking the medicine, the patient’s own body can be activated by the laser to produce certain substances that can often replace the drugs. This is a literature review emphasizing the use of low level laser. Thus, the reflections from this study can contribute to the amplification of the production of knowledge that aims at the resignification of the use of laser in therapy. Greater attention was given to two types of laser radiation: He-Ne and As-Ga, both presenting a high therapeutic potential in superficial and deep injures. However, the He-Ne laser excels in superficial lesions, whereas the As-Ga laser does so in deep injures, such as articular, muscular, etc.

关键词： Laser Injury Regeneration

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Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy

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Advances in Bioscience and Biotechnology 2014年第11期5卷 903-914页

作者： Dayse Drielly Souza Santana-Vieira Debora Marcondes Bastos pereira Milori Paulino Ribeiro Villas Boas Marina Franca e Silva Magno Guimaraes santos Fernanda Amato Gaiotto Walter dos santos Soares Filho Abelmon da Silva Gesteira Embrapa Cassava and Fruits Cruz das AlmasBrazil Department of Biological Sciences State University of Santa CruzIlheusBrazil Embrapa Instrumentation Sao CarlosBrazil

The differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties.

关键词： Differentiation of Citrus Genotypes Sunki LIFS Molecular Markers PLSR

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Assessment of Acute Episodes in Chronic Stable Angina: A Clinical-Laboratory Approach to the Use of the Coronary Vasodilator Propatyl Nitrate

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World Journal of Cardiovascular Diseases 2016年第7期6卷 246-252页

作者： Gerson Goldwasser Renato Kaufman Carlos pereira Nunes Alexandre Hid Stephanie Wrobel Goldberg Alessandra santos Lisa Oliveira Adenilson de Souza da Fonseca Mauro Geller Souza Marques Medical School Rio de Janeiro Brazil Universidade Estadual do Rio de Janeiro Rio de Janeiro Brazil UNIFESO Medical School Teresópolis Brazil Washington University School of Medicine St. Louis MO USA

Background/Objectives: Propatyl nitrate is a coronary vasodilator with immediate and prolonged action, indicated in the treatment and prevention of acute angina pectoris episodes. Methods: This was an open, self-paired comparative study performed at UNIFESO Medical School evaluating the clinical and laboratory results of treatment with propatyl nitrate in patients with chronic stable angina pectoris. Subjects received 10 mg of propatyl nitrate, at the dose of three sublingual tablets per day, to be taken at 8:00 A.M., 2:00 P.M., and 8:00 P.M. Subjects returned to the study center after 15 days of treatment for Visit 2 assessments, and at the end of the 30-day treatment period (Visit 3). Results: A total of 200 subjects were included in the study. There was a statistically significant reduction in blood pressure (p < 0.0001) and heart rate (p = 0.0001), but no change in respiratory rate (p = 0.23). Laboratory results did not vary throughout the treatment period. There was no significant change from pretreatment in the SAQ Physical Limitation scale (p = 0.7415). The Angina Stability, Angina Frequency, and Treatment Satisfaction, and Quality of Life scales showed a significant improvement from pretreatment (p < 0.0001). Adverse events were observed among 41 subjects at Visit 2 and 35 subjects at Visit 3. Conclusions: Propatyl nitrate was safe and effective in treating chronic stable angina pectoris over the course of the 30-day treatment period. Treatment with propatyl nitrate increased angina stability and reduced angina frequency while increasing treatment satisfaction and quality of life in the patient population evaluated.

关键词： Propatyl Nitrate Stable Chronic Angina Pectoris Seattle Angina Questionnaire

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

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Journal of Integrative Medicine 2019年第2期17卷 132-140页

作者： Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia Adolfo Erustes Alexandre Bechara Caroline Palmeira-dos-santos Soraya S.Smaili Gustavo J.S.pereira André Hinsberger Ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto Departamento de Farmacologia Escola Paulista de Medicina (EPM) Universidade Federal de Sao Paulo (UNIFESP) Grupo de Fitocomplexos e Sinalizacao Celular Escola de Ciências da Saúde Universidade Anhembi Morumbi Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz Laboratório de Produtos Naturais e Quimiometria Programa de pós-graduacao em Farmácia e Biotecnologia Universidade Anhanguera de Sao Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual de Campinas (UNICAMP) Unidade de Oncologia Experimental EPM Universidade Federal de Sao Paulo (UNIFESP)

Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its *** study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast ***: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo ***: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated ***: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy.

关键词： Angelica archangelica Umbelliferae Apoptosis Cell death Breast cancer Angelicin

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Production of Peanut Intercropped with Forage Palm in Pernambuco State, Brazil

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American Journal of Plant Sciences 2015年第6期6卷 818-825页

作者： Juliana Aparecida santos Andrade Jeandson Silva Viana José Jairo Florentino Cordeiro Junior Abraao Cicero da Silva Edilma pereira Goncalves Djayran Sobral Costa Academic Unit Garanhuns Federal Rural University of PernambucoRecifeBrazil

The greater use of crop fields by increasing plant population is an alternative to minimize losses to the farmers who use crop intercropped provides other benefits such as lower incidence of diseases and pests, and better exploitation and enrichment agroecosystem. The objective of this study was to evaluate the effect of the population of peanuts with and without inoculation and intercropping with forage palm on growth, productivity and biomass peanut. The treatments consisted of different populations of peanut (one, two and three rows of peanut row spacing of two meters of cactus pear), with and without inoculant, analyzed in subdivided plot scheme (3 × 2), totalizing six treatments in a randomized block design with four replications. The variables evaluated were plant height, number of branches, shoot dry mass and root nodule number, nodule dry weight, biomass productivity and legumes. The largest population of plants in number of lines of peanut intercropped with forage cactus promotes greater total biomass production and yield of beans and does not alter the height, branch number, dry mass of aerial part roots and nodules. The inoculant application did not result in to increased productivity biomass and growth of peanut plants, their use is not indicated. The planting of a row of peanuts between the rows of palm is not economically recommended.

关键词： Arachis hypogaea L. Inoculation Population Nopalea cochenillifera

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Accessory Placental Structures—A Review

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Open Journal of Animal Sciences 2014年第5期4卷 305-312页

作者： Rennan Lopes Olio Luis Miguel Lobo Márcio Aparecido pereira Amilton Cesar santos Diego Carvalho Viana Phelipe Oliveira Favaron Maria Angelica Miglino Department of Surgery School of Veterinary Medicine and Animal Science University of Sao Paulo Sao Paulo Brazil

Many studies have reported on the different types of placenta in different species, but accessory or paraplacental structures are mentioned only in species that have such a nutrient-exchanging mechanism. Hystricomorph rodents possess a subplacenta, swine and equines have the placental areola, equines have the chorionic girdle, and carnivores have hemophagous organs that are equivalent to the placental hematoma in ruminants. These accessory structures are specialized tissues for specific nutrient exchanges in different species, and they are adaptive modifications exhibited by domestic and wild species to remedy the nutritional deficiencies that are related to permeability of the main placenta to important nutrients for the growth and development of the fetus during the gestation.

关键词： Placental Areola Chorionic Girdle Placental Hematoma Hemophagous Organs Subplacenta

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Sample Dimension for Evaluating Characters of Yellow Mombin

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Agricultural Sciences 2019年第8期10卷 1032-1038页

作者： Omar Schmildt Vinicius de Souza Oliveira Renan Garcia Malikouski Adriel Lima Nascimento Karina Tiemi Hassuda dos santos Hérica Chisté Gleyce pereira santos Marcio Paulo Czepak Rodrigo Sobreira Alexandre Edilson Romais Schmildt Postgraduate Program in Tropical Agriculture Federal University of Espírito Santo S&#227 o Mateus ES Brazil Departament of Agrarian and Biological Sciences Federal University of Espírito Santo S&#227 o Mateus ES Brazil Departament of Forest and Wood Science Federal University of Espírito Santo Jer&#244 nimo Monteiro ES Brazil

The objective of this study was to determine the sample size needed to evaluate characters of mature fruits of yellow mombin (Spondias mombin L.). 100 fruits were evaluated from plants grown under a shading system. In the laboratory, these fruits were measured by the characters: longitudinal length (mm);equatorial diameter (mm);fruit mass (g);yield of pulp (%);total soluble solids—TSS (°Brix);titratable acidity—TA (% citric acid) and ratio (TSS/TA), being calculated the measures of central tendency and dispersion, and verified normality. Next, the sample size for each character was determined. For experimental evaluation of cajámirim fruits, considering an accuracy of 10% around the mean, 40 fruits per treatment should be evaluated, considering a completely randomized experiment.

关键词： Spondias mombin L. Error of Estimation Sampling

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Decommissioning of Mature Oil Fields and Artisanal Fisheries： The Case of Todosos santos Bay, Brazil

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Journal of Environmental Science and Engineering(A) 2012年第1期1卷 73-81页

作者： Henrique de Barros Marise Paranagua viviane Almeida Mauro MeloJunior Patricia Ribeiro DLCH-Federal Rural University of Pernambuco Recife 52171-900Brazil Federal Rural University of Pernambuco Recife 52171-900Brazil Secretaria de MeioAmbiente Prefeitura do Recife 50030-230Brazil UAST-Federal Rural University of Pernambuco Recife 52171-900Brazil

Consequences of decommissioning oil fields on artisanal fishing activities are still little known in the literature. This paper is intended to shed some light on a process of dismantling and sinking of oil and gas structures in shallow waters, with severe disturbing impacts on low income artisanal fishing activities. From a socio-economic perspective, the relationship of oil industry with local communities is described, with the main perceived problems pointed out in local fishermen leadership perspective. The notions of ＂damages＂ and ＂mitigation＂ used by the oil industry are discussed in connection to the expansion and dismantling of oil installations during the past 20 yrs. A comparative view of oil fields decommissioning in Europe and Brazil during the late 1990s suggests the need to review transparency and social commitment standards which have been far less prominent in this Brazilian case. The authors believe that the Brazilian oil industry has acquired a social and environmental debt towards the whole society, as far as it has been unable to establish a clear and effective process for decommissioning their oil installations within the artisanal fishing areas of the Todosos santos Bay. Furthermore, the discussion of fair and specific compensations has been avoided, which otherwise would be instrumental to regain local economic conditions found among fishermen just few decades ago.

关键词： Decommissioning oil fields mature oil fields environmental damages fishing communities Todosos santos Bay.

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Application of Non-destructive Testing to Evaluate the Performance of Bridges Structures

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Journal of Civil Engineering and Architecture 2017年第1期11卷 1-7页

作者： Diego Jesus de Souza Elaine Souza dos santos Eric Pianizolli Flor Gabriel pereira Marinho Giovana Costa Reus Heloise Cristine Cezario Leticia Andrade Camara Department of Civil Engineering University of Ottawa Ottawa K2P0M1 Canada Department of Civil Construction Universidade Federal do Parana Sao Jose dos Pinhais 83040-334 Brazil Department of Civil Construction Universidade Federal do Parana Linhares 29902-190 Brazil Department of Civil Construction Universidade Federal do Parana Curitiba 80630-280 Brazil Department of Civil Construction Universidade Federal do Parana Campo Largo 83604-230 Brazil Department of Civil Construction Universidade Federal do Parana Curitiba 80060-120 Brazil

Bridges are important elements in road system and can influence the entire economy of cities and region. Usually, these structures have high financial investments for their constructions, in this way, maintenance and conservation become so important. Inspection is a technical activity that covers several operations, including performance analysis; examination; final performance report, other operations may be necessary, such as maintenance work, recovery, strengthening and rehabilitation. Technical examination together with some test methods allows a critical and parametric judgment of the bridge performance by minimizing the subjectivity of visual evaluations, and permits a more detailed diagnosis. This paper discusses the methodology to perform tests to complement the assessment recommended by DNIT （Brazilian National Department of Transport Infrastructure）, which consists only on a visual judgment. This approach provides technical basis to classify the bridges as its need and urgency of maintenance.

关键词： Non-destructive testing bridges concrete durability.

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Root Growth, Nutrition and Yield of Maize with Applied Finely Limestone in Surface of Cerrado Soil

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American Journal of Plant Sciences 2014年第6期5卷 834-844页

作者： Rafael Felippe Ratke Hamilton Seron pereira Joao de Deus Gomes dos santos Junior Joaquim JoséFrazao Juliano Magalhaes Barbosa Bruno de Oliveira Dias Departamento de Engenharias AgronomiaCampus Professora Cinobelina ElvasUniversidade Federal do PiauíBom JesusBrazil Instituto de Ciencias Agrárias Universidade Federal de UberlandiaUberlândiaBrazil Embrapa Centro Nacional de Pesquisa no CerradoPlanaltinaBrazil Programa de Pós-graduacao em Agronomia Universidade Federal de GoiásGoianiaBrazil

The surface applies lime to correcting soil acidity in no-tillage and promotes root development and yield of maize. However, limestone with a particle size smaller than 0.30 mm cannot promote this effect. The aim of this study was to determine the root attributes, nutrition and productivity of maize with limestone ground in no-tillage. The experimental design was randomized blocks with five treatments and four replications. The treatments consisted of different particle sizes of limestone applying superficially to the soil under NT 0) control, without lime, 1) 0.30 mm to 0.20 mm 2) 0.20 mm to 0.10 mm, 3) 0, 10 mm to 0.05 mm 4) 0.05 mm to 0.01 mm. Attributes root, leaf nutrition and grain yield of maize were evaluated in two years 2009 and 2010. The elongation of roots in a 20-40 cm depth was greater when using limestone with particle size between 0.30 mm and 0.20 mm. The root length of maize plants correlated with the levels of Mn in maize leaves. The root development promoted by liming effect was not reflected in the productivity of maize.

关键词： Particle Size No-Tillage Limestone

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