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Production of chitinases with Trichoderma harzianum isolates using solid substrate fermentation

浙江大学学报(农业与生命科学版) 2004年第4期 85-85页

作者： viviana nagy,gyorgy szakacs,ashok pandey Technical University of Budapest Department of Agricultural Chemical Technology 1111 Budapest 4] Szent Gellert ter 9] Szent Gellert ter Hungary Technical University of Budapest Department of Agricultural Chemical Technology 1111 Budapest Hungary Regional. Research Laboratory Biotechnology Division CSIR Trivandrum-695 019 India

Over forty Trichoderma harzianum isolates have been screened in solid substrate fermentation (SSF) for chitinase production. Strains were isolated from Asian soil and tree bark samples. Identification was performed in Canada and Austria by classical and molecular taxonomical methods. Four SSF media were used for the screening. They contained wheat bran, crude chitin from crab shells (SIGMA) and different salt solutions for wetting of the substrate. In a five day fermentation at 30°C the best chitinase producers were T. harzianum TUB F-691, TUB F-693, TUB F-699, TUB F-700, TUB F-927, TUB F-947 and TUB F-972 isolates (TUB = Technical University of Budapest culture collection) . The best producers yielded between 3.5-5.5 International Unit/g dry matter (DM) chitinase. T. harzianum TUB F-947 was selected for further optimization. This strain was isolated from a soil of Thailand. The optimum moisture content (67%), optimal alternative substrate-carrier for SSF (wheat straw) and chitin content of the substrate (30%) were determined. Temperature and pH optima of chitinase produced by T. harzianum TUB F-947 were found to be at 50°C and 5.0, respectively. The enzyme complex was thermally not stable at 50 ℃. Medium optimization experiments to enhance the chitinase production using statistical methods also have been performed.

关键词： Trichoderma harzianum chitinase solid substrate fermentation

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Production of chitinases with Trichoderma harzianum isolates using solid substrate fermentation

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浙江大学学报（农业与生命科学版） 2004年第4期30卷 435-435页

作者： viviana nagy gyorgy szakacs ashok pandey Technical University of Budapest Department of Agricultural Chemical Technology 1111 Budapest Szent Gellert ter 4. HungaryTechnical University of Budapest Department of Agricultural Chemical Technology 1111 Budapest Szent Gellert ter 4. Hungary Regional. Research Laboratory Biotechnology Division CSIR Trivandrum-695 019 India

关键词：几丁质酶固态基质发酵木霉属真菌

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