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寄生草本植物大花菟丝子的研究综述(英文)

中西医结合学报 2012年第3期10卷 249-255页

作者： Satish Patel Vikas Sharma Nagendra S.Chauhan vinod k.dixit Department of Pharmaceutical Sciences Dr.Hari Singh Gour University

大花菟丝子是一种金黄色、无叶、多年生寄生草本植物,它隶属于旋花科,俗称发菜、鬼肠等。经研究,大花菟丝子具有止痉、抗惊厥、抗类固醇生成、降血压、助肌肉松弛、强心、利尿、抗病毒、抗菌、抗氧化以及助毛发生长等一系列药用作用。... 详细信息

大花菟丝子是一种金黄色、无叶、多年生寄生草本植物,它隶属于旋花科,俗称发菜、鬼肠等。经研究,大花菟丝子具有止痉、抗惊厥、抗类固醇生成、降血压、助肌肉松弛、强心、利尿、抗病毒、抗菌、抗氧化以及助毛发生长等一系列药用作用。目前从大花菟丝子中提取的有效化学成分有岩白菜素、阿马别林、β-谷甾醇、豆甾醇、山奈酚、半乳糖醇、杨梅酮、槲皮素、香豆素和齐墩果酸等。本文概述了生药学和植物化学文献中有关大花菟丝子的研究结果,并从传统医学和生物学角度评价了它的医药用途。

关键词：菟丝子属旋花科植物药用类黄酮物质综述

Enhanced recovery after surgery protocol enhances early postoperative recovery after pancreaticoduodenectomy

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Hepatobiliary & Pancreatic Diseases International 2019年第2期18卷 188-193页

作者： Ramasamy Mahendran Mallika Tewari vinod kumar dixit Hari Shankar Shukla Department of Surgical Oncology Institute of Medical Sciences Banaras Hindu University Department of Gastroenterology Institute of Medical Sciences Banaras Hindu University

Background: Enhanced recovery after surgery(ERAS) protocol is a multimodal, multidisciplinary and evidence-based approach to reduce surgical stress and enhance recovery in the postoperative period. This study aimed to analyze the outcome of ERAS protocol in patients after pancreaticoduodenectomy(PD). Methods: A total of 50 consecutive patients with pancreatic/periampullary cancer who underwent PD between January 2016 to August 2017 were included in the study. As per the institute ERAS protocol, nasogastric tube(NGT) was removed on postoperative day(POD) 1 if output was less than 200 mL and oral sips were allowed; oral liquids were allowed on POD2; semisolid diet by POD3; abdominal drain was removed on POD 4 if output was less than 100 mL with no evidence of postoperative pancreatic fistula(POPF); normal diet was allowed on POD5. Discharge criteria on POD6 were afebrile, tolerating oral normal diet, pain free and no surgery related complications(defined as per the ISGPS definitions). Results: NGT was removed on POD1 in 45(90%) patients, abdominal drain removed by POD4 in 41(82%) and 43(86%) patients were discharged on POD6. There was no 30-day postoperative mortality. Three(6%) patients had delayed gastric emptying(DGE). None had postoperative hemorrhage and POPF. Readmission rate was 8%. A significant relation was found between the length of hospital stay(LOS) with age( P < 0.05) and a marginal relation between LOS and postoperative albumin( P = 0.05). Conclusions: ERAS protocol can be safely followed in the perioperative care of patients who undergo PD. Early removal of NGT and allowing oral diet restore bowel function early. ERAS decreases the LOS and postoperative complications.

关键词： Enhanced recovery protocol Pancreatic cancer Periampullary cancer Pancreaticoduodenectomy Early discharge

Effect of Chlorophytum Borivilianum Santapau and Fernandes on Sexual Dysfunction in Hyperglycemic Male Rats

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Chinese Journal of Integrative Medicine 2009年第6期15卷 448-453页

作者： Mayank Thakur Shilpi Bhargava Werner Praznik Renate Loeppert vinod k.dixit Department of Pharmaceutical Sciences Dr. Hari Singh Gour University Department of Chemistry University of Natural and Applied Life Sciences Dr.Hari Singh Gour University

Objective:To investigate the effects of Chlorophytum borivilianum(CB) on sexual dysfunction, loss of body weight,and lack of libido in hyperglycemic rats induced with streptozotocin or ***: Wistar strain male albino rats were divided into five groups of six animals each:the control group(2% polyvinylpyrollidone solution),the streptozotocin control group(50 mg/kg),the alloxan control group(100 mg/kg), the streptozotocin + CB treated group(200 mg/kg),and the alloxan + CB treated group(200 mg/kg).O...

关键词： ayurvedic rasayana Chlorophytum borivilianum diabetes induced sexual dysfunction Safed Musli

Culture Medium Optimization for Enhanced Puerarin Production by Shake Flask Cultures of Pueraria tuberosa(***.) DC

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Culture Medium Optimization for Enhanced Puerarin Production...

第二届国际整合植物生物学学术研讨会

作者： Vijai Singh karwasara vinod kumar dixit Department of Pharmaceutical Sciences Dr.H.S.Gour VishwavidyalayaSagarMadhya Pradesh 470003India

Pueraria tuberosa DC(Fabaceae) is a perennial woody lianas which is highly valued in Ayurveda and traditional Chinese systems of *** major constituents of Pueraria tuberosa were isoflavonoids,mainly puerarin,an isoflavonoid C-glycoside found in the genus *** exhibits a great variety of biological and pharmacological *** is used clinically for treatment of cardiac and cerebral blood vascular *** has been shown to possess many pharmacological activities such as antioxidant activity,anti-inflammatory activities,and protective activity against ischemic *** recent investigations reported puerarin could induce apoptosis in cancer *** cell cultures remain a favoured methodology for biotechnological production of nature-based compounds despite large-scale destruction of natural habitat due to higher demand and overexploitation of ***,the conventional propagation of Pueraria is too *** is disparity in puerarin content due to varying seasonal and agro-ecological ***,our aim is to establish the in-vitro culture of Pueraria,and to optimize the effect of essential macro and micro-nutrient components and sucrose feeding on P. tuberosa cell growth and isoflavonoid *** the initiation of *** culture leaves, stems and petioles were used with different growth medium and varying concentrations of different plant growth *** media supplemented with 4.52 uM 2,4-D + 5.37 uM NAA + 4.65μM kn showed the highest callogenesis *** month old friable callus cultures were used to initiate suspension *** estimation of puerarin was done by HPLC. The effects of carbon,nitrogen,phosphate,and copper on cell growth and production of the isoflavone puerarin by suspension cultures of Pueraria tuberosa was *** feeding strategy remarkably stimulated cell biomass growth and puerarin *** was also noticed that NH or NO as the solitary nitr

关键词： cell suspension culture macro-nutrient optimization Pueraria tuberosa puerarin

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仙茅:对健康有众多益处的黑金(英文)

中西医结合学报 2010年第7期8卷 613-623页

作者： Nagendra Singh Chauhan Vikas Sharma Mayank Thakur vinod kumar dixit Department of Pharmaceutical Sciences Dr.Hari Singh Gour University

仙茅(石蒜科)是一种濒临灭绝的多年生草本植物,是印度本土药,作为强有力的适应原和春药在印度传统药物中占有重要位置。它也是多种印度传统药物处方的重要组成部分,被认为具有促进性欲、刺激免疫、保护肝脏、抗氧化、抗癌和治疗糖尿病... 详细信息

仙茅(石蒜科)是一种濒临灭绝的多年生草本植物,是印度本土药,作为强有力的适应原和春药在印度传统药物中占有重要位置。它也是多种印度传统药物处方的重要组成部分,被认为具有促进性欲、刺激免疫、保护肝脏、抗氧化、抗癌和治疗糖尿病等作用。已有报道,从仙茅中提取出多种化学成分,如植物黏胶,酚苷、皂苷和脂肪族化合物等。仙茅也是中医和日本汉方医学中各种草药处方的重要成分。本文对仙茅的多种生物活性和其不同化学成分的作用进行了综述。

关键词：仙茅催欲药医学,印度传统提取物

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提高认知能力的印度传统草药土丁桂(英文)

中西医结合学报 2009年第11期7卷 1001-1022页

作者： Neeraj kumar SETHIYA Alok NAHATA Sri Hari MISHRA vinod kumar dixit Herbal Drug Technology Labaratory Department of Pharmacy the Maharaja Sayajirao University of Baroda Vadodara Department of Pharmaceutical Sciences Dr. Hari Singh Gour Vishwavidyalaya Herbal Drug Technology Labaratory Department of Pharmacy the Maharaja Sayajirao Uniersity of Baroda

土丁桂属草药是作用于中枢神经系统的印度传统药物,特别是对促进记忆和改善智力有较好疗效。从印度传统医学和梵语文献中提取的大量信息提示,冠以土丁桂属草药名称的植物类别共4种:旋花科田旋花、旋花科土丁桂、蝶形花科蝴蝶花豆和龙胆... 详细信息

土丁桂属草药是作用于中枢神经系统的印度传统药物,特别是对促进记忆和改善智力有较好疗效。从印度传统医学和梵语文献中提取的大量信息提示,冠以土丁桂属草药名称的植物类别共4种:旋花科田旋花、旋花科土丁桂、蝶形花科蝴蝶花豆和龙胆科穿心草。这些草药名称均在古代文献中有所记载,可单独使用或与其他药材组合成各种草药处方。本文就现有的科学信息,如土丁桂属不同植物来源物种的生药学特征、化学成分、药理作用、临床前及临床应用等方面进行述评,以期为科学地应用土丁桂提供依据。此外,还可根据不同植物来源土丁桂属草药的不同功效进行鉴别应用。

关键词：旋花科田旋花旋花科土丁桂蝶形花科蝴蝶花豆龙胆科穿心草医学印度传统认知障碍

南欧派利吞草石油醚提取物对雄性大鼠性行为的影响(英文)

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中西医结合学报 2010年第8期8卷 767-773页

作者： Vikas Sharma Mayank Thakur Nagendra S.Chauhan vinod kumar dixit Department of Pharmaceutical Sciences Dr.Hari Singh Gour Vishwavidyalaya

目的:检测南欧派利吞草石油醚提取物(petroleum ether extract,PEE)对雄性大鼠性行为的影响。方法:制备南欧派利吞草PEE。32只雄性Wistar大鼠分成对照组、睾酮组、低剂量PEE组和高剂量PEE组,每组8只。对照组大鼠灌胃花生油(溶剂对照),... 详细信息

目的:检测南欧派利吞草石油醚提取物(petroleum ether extract,PEE)对雄性大鼠性行为的影响。方法:制备南欧派利吞草PEE。32只雄性Wistar大鼠分成对照组、睾酮组、低剂量PEE组和高剂量PEE组,每组8只。对照组大鼠灌胃花生油(溶剂对照),睾酮组大鼠肌肉注射0.5mg/kg睾酮,每周2次,低剂量和高剂量PEE组大鼠分别灌胃50和100mg/kgPEE,治疗持续28d。于治疗前,治疗第15天、第28天和治疗后第7天、第15天,检测大鼠体质量,治疗结束后,取4只大鼠性器官(睾丸、附睾、精囊、前列腺)称取质量,其余4只大鼠用于检测大鼠的性取向行为和性功能。结果:与对照组大鼠比较,PEE治疗28d可明显增加雄性大鼠的体质量和性器官质量,雄性大鼠易于接受雌性大鼠和对雌性大鼠趋向性增加,雄性大鼠性能力增加,表现为舔、嗅雌性大鼠肛门和生殖器,阴茎勃起指数增加,交配潜伏期缩短,交配频率增加。在PEE治疗结束后7和15d仍观察到以上行为和性指标的变化。结论:与睾酮不同的是,PEE治疗结束后7和15d仍可见明显治疗效果,提示南欧派利吞草能提高大鼠性潜能,并有延迟效应。

关键词：南欧派利吞草催欲药植物提取物石油醚提取物性行为,动物烷基酰胺大鼠

Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study

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World Journal of Gastroenterology 2013年第7期19卷 1056-1067页

作者： Saurabh kumar Patel Chandra Bhan Pratap Ajay kumar Verma Ashok kumar Jain vinod kumar dixit Gopal Nath Department of Microbiology Institute of Medical SciencesBanaras Hindu UniversityVaranasi UP 221005India Department of Gastroenterology Institute of Medical SciencesBanaras Hindu UniversityVaranasi UP 221005India

AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic ***:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic *** selected isolates were further characterized by molecular techniques *** for 16S rRNA using universal eubacterial and HSP60 gene specific *** amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of *** of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/***:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(***) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight *** partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(***).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be ***,and HSP60 sequences had more discriminatory potential rather than 16S rRNA ***,this bacterium was acid tolerant for hours at low ***,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targetin

关键词： Antral biopsy Helicobacter pylori Pseudomonas fluorescens HSP60 Nested polymerase chain reaction Acid-tolerant bacteria