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Reproductive biology of Cornus capitata Wall. ex Roxb.: a native species in East Asia

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Journal of Forestry Research 2019年第6期30卷 2039-2050页

作者： vinod Prasad Khanduri Arun Sukumaran Chandra Mohan Sharma College of Forestry VCSG Uttarakhand University of Horticulture and Forestry

The reproductive biology of Cornus capitata was studied in detail from June 2014 to October 2015 in Tehri Garhwal, Uttarakhand, western Himalaya, India and elucidated floral morphology, confirming autogamy, geitonogamy, and xenogamy. A new inflorescence ball formed each year with developing fruits that were initiated the previous year; thus reproduction is a continuous cycle. The total pollen production within a tree ranged between 4.0 × 10~7 and 9.3 × 10~7. Anthesis peaked between 08:00 and 10:00. There was significant effect of micro-environmental condition on anthesis(F = 243.8, p = 3.01). A significant positive relationship between the number of lateral shoots and total inflorescence showed that the inflorescence production in C. capitate depends largely on the number of lateral shoots. Time and concentration of various solutions significantly affected pollen germination(time: F = 5.39, p = 0.002; concentration: F = 1.40,p = 0.234). Anthophora bees and thrips were observed as the main pollinators. There was a significant difference in seed set between natural and obligate self-pollination(t = 11.84, p = 1.99). Significant effect was also observed on fruit size(t = 8.88, p = 2.09) between open and shaded conditions. SEM micrographs of pollen grains showed that the shape of dehydrated pollen grains differed greatly due to variations in the rate of dehydration among pollen grains within the anther. Seed germination was observed only in quality seed derived from open pollination, supporting xenogamy in C. capitata. Findings of the present study will be of immense value in formulating effective conservation measures for the species in wild habitats.

关键词： Floral development Pollen production Pollination Pollinators Reproductive success SEM analysis

Post COVID-19 mucormycosis:A case series

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Asian Pacific Journal of Tropical Medicine 2021年第11期14卷 517-524页

作者： Mala vinod Kaneria Kallappa Baligeri Abhijeet Budhe Department of Medicine Topiwala National Medical College and B.Y.L.Nair Ch.HospitalMumbaiIndia

Objective:To evaluate the associated factors between COVID-19 and ***:Twenty-two patients of COVID-19 associated mucormycosis(including 3 asymptomatic patients who were cured of COVID-19)from a single medical unit of our institute were included.A detailed history was noted,with special emphasis on the time of onset of mucormycosis symptoms,presence of comorbidities,including new onset diabetes,severity of COVID-19,oxygen requirement,details of receipt of steroids and immunomodulators such as tocilizumab,imaging findings,including the number of sinuses involved,bony erosions,orbital and cerebral involvement,microscopy,culture and histopathology reports and antifungals *** interventions including number of debridements,orbital exenteration,maxillectomy,and the vaccination status were ***:All 22 patients had rhino-orbital cerebral mucormycosis,27.27%in the first wave and 72.73%during the second *** was the commonest comorbidity,and 40.91%patients were newly detected *** time of presentation in relation to their COVID-19 symptoms was 8-15 days(average 12.5 days).Ten out of 22(45.45%)had asymptomatic or mild COVID-19 and 40.91%did not require supplemental *** out of 22(22.73%)did not receive *** out of 22(54.55%)had orbital involvement,3(13.64%)had palatal ulcer and 4(18.18%)had cerebral involvement and all these had progressed in spite of treatment with appropriate ***:COVID-19 associated mucormycosis is a frequent,lethal,post COVID-19 complication,occurring even in mild and asymptomatic cases who have not received steroids or oxygen.

关键词： Mucormycosis COVID-19 Steroids Amphotericin B

Temporal and spatial variation of pollen yield in natural populations of Pinus roxburghii

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Forestry Studies in China 2012年第1期14卷 20-29页

作者： vinod Prasad KHANDURI Department of Forestry Mizoram UniversityTanhrilAizawl 796001India

Male components of the reproduction process in Pinus roxburghii were investigated for their variation in time and space as well as pollination, viz. flowering phenology, pollen production variability and pollen dispersal, for five successive years at two different locations （at the lower and higher elevations）. The study reveals that elevation and the hour of the day are the main determi- nants of anthesis and microsporangium dehiscence, because both are related to temperature and humidity. The receptivity of ovulate cone strobili or female strobili occurred earlier at the lower elevation than at the higher elevation, with a longer receptivity period in the latter case. The phenology of male and female cones varied significantly between years and elevations. The determinations of pol- len yield considered various sources of variability, i.e., the number of pollen strobili per branch, strobili per tree, microsporangia per tree and pollen grains per tree. Each of these parameters revealed significant year-to-year and elevation effects. Year-to-year varia- tion in the production of pollen cone and pollen grains with mass production after a three-year period revealed a three-year cycle of masting in pollen production in P. roxburghii. The pollen dispersal decreased quickly with distance from the pollen source. As a con- sequence, a distance of 600 m was proposed as a minimum to prevent contamination by pollen in the management of seed orchards. The results ultimately suggest that the ample production of pollen grains per tree along with flowering synchrony and long dispersal of pollen grains results in homogeneity in large populations.

关键词： chir pine pollen cone gene flow elevation Himalayas

Pollinator-mediated self-pollination and reproductive assurance in an isolated tree of Magnolia grandiflora L.

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Ecological Processes 2020年第1期9卷 517-525页

作者： Arun Sukumaran vinod Prasad Khanduri Chandra Mohan Sharma College of Forestry VCSG Uttarakhand University of Horticulture and ForestryRanichauriTehri GarhwalUttarakhand 249199India

Background:The ability to produce seeds when pollinators or potential mates are scarce is one of the principal advantages of self-pollination in flowering ***,the role of pollinators mediating self-pollination to ensure seed set when pollen or potential mates are limited has received less *** study examined the reproductive consequences and involvement of pollinators in assuring seed set in an isolated tree of Magnolia grandiflora,a predominantly outcrossing ***:We tested various aspects of reproduction such as flower density,floral rewards,stigma receptivity,pollinator abundance and behaviour,fruit set and seed set,in two successive reproductive years(2015-2016).Results:Flowers of *** possess a suite of traits facilitating pollinator-mediated self-pollination(PMS),although the chances of autonomous self-pollination are reduced due to herkogamy,spatial separation of anther and *** mean pollen production/flower was 5,152,289±285,094 with a pollen-to-ovule ratio of 39430±*** found a significant positive correlation between number of visits and seed production for bees(r=0.5099,p=0.0007)and beetles(r=0.7159,p=0.00001),indicating these are effective at *** was a significant negative correlation for thrips(r=-0.3206,p=0.044)and no correlation for flies or *** percent fruit set was 100%and the seed set per ovule ranged between 19 and 20%.Conclusions:PMS will guarantee reproductive assurance and mitigate the effect of reduced mates or pollen limitation which is expected to increase the fecundity and establishment of individual trees in geographically isolated locations.

关键词： Pollinator-mediated self-pollination Magnolia grandiflora Autonomous self-pollination Pollinator behaviour Reproductive assurance

SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis

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Molecular Plant 2016年第7期9卷 1051-1065页

作者： Souha Berriri Sreeramaiah N Gangappa and S vinod Kumar Cell and Developmental Biology Department John Innes Centre Norwich NR4 7UH UK

Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWRlc and H2A.Z have been shown to con- trol gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well under- stood. In this study, we analyzed the roles of the SWRlc subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 （PIE1）, ACTIN-RELATED PROTEIN6 （ARP6）, and SWR1 COMPLEX 6 （SWC6）, as well as H2A.Z, in defense and gene regulation. We found that SWRlc components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWRlc components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expres- sion analyses similarly reveal distinct roles for H2A.Z and SWRlc components in gene regulation, and sug- gest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWRlc components could have non-redundant functions in plant immunity and gene regulation.

关键词： arabidopsis SWR1 chromatin remodeling H2A.Z immunity gene regulation

Ophiolites-Geodynamic and environmental implications:Preface

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Geoscience Frontiers 2020年第1期11卷 1-2页

作者： Emilio Saccani vinod Samuel M.Santosh Department of Physics and Earth Sciences Ferrara University44122FerraraItaly Department of Earth System Sciences Yonsei UniversitySeoulSouth Korea China University of Geosciences(Beijing) Beijing 100083China

Ophiolite studies over the decades have played a crucial role in better understanding of mid-ocean ridge and subduction zone processes,mantle dynamics and heterogeneity,fluid-rock interactions in both sub-oceanic and sub-arc upper mantle,and the mechanisms of continental growth in accretionary and collisional *** continued interest in ophiolites as a topic of multi-disciplinary research in Earth Sciences has resulted in a wealth of new data,syntheses,and ideas from ophiolites around the world.

关键词： mantle rock Earth

QTL and QTL × Environment Effects on Agronomic and Nitrogen Acquisition Traits in Rice

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Journal of Integrative Plant Biology 2008年第9期50卷 1108-1117页

作者： Senapathy Senthilvel Kunnummal Kurungara vinod Palaniappan Malarvizhi Marappa Maheswaran Centre for Plant Breeding and Genetics Tamil Nadu Agricultural University Department of Soil Science and Agricultural Chemistry Tamil Nadu Agricultural University Centre for Plant Molecular Biology Tamil Nadu Agricultural University

Agricultural environments deteriorate due to excess nitrogen application. Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input. Rice genotypes respond variably to soil available nitrogen. The present study attempted quantification of genotype x nitrogen level interaction and mapping of quantitative trait loci （QTLs） associated with nitrogen use efficiency （NUE） and other associated agronomic traits. Twelve parameters were observed across a set of 82 double haploid （DH） lines derived from IR64/Azucena. Three nitrogen regimes namely, native （0 kg/ha; no nitrogen applied）, optimum （100 kg/ha） and high （200 kg/ha） replicated thrice were the environments. The parents and DH lines were significantly varying for all traits under different nitrogen regimes. All traits except plant height recorded significant genotype × environment interaction. Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake. Sixteen QTLs were detected by composite interval mapping. Eleven QTLs showed significant QTL × environment interactions. On chromosome 3, seven QTLs were detected associated with nitrogen use, plant yield and associated traits. A QTL region between markers RZ678, RZ574 and RZ284 was associated with nitrogen use and yield. This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes.

关键词： doubled haploid population nitrogen acquisition quantitative trait loci quantitative trait loci x environment interaction rice.

Hot n＇ Cold： Molecular Signatures of Domestication Bring Fresh Insights into Environmental Adaptation

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Molecular Plant 2015年第10期8卷 1439-1441页

作者： Catherine Gardener S. vinod Kumar Cell and Developmental Biology Department John Innes Centre Norwich NR4 7UH UK

Recent investigations of divergent rice genotypes have identified previously unknown molecular adaptations conferring heat and chilling tolerance in their corresponding local environments （Figure 1）. These studies s... 详细信息

关键词： domestication environmental adaptation

Au, Ag and Au:Ag colloidal nanoparticles synthesized by pulsed laser ablation as SERS substrates

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Progress in Natural Science:Materials International 2014年第6期24卷 569-578页

作者： M.vinod K.G.Gopchandran Department of Optoelectronics University of Kerala

Chemically pure colloidal suspensions of gold and silver nanoparticles were synthesized using pulsed laser *** dependence of laser fl uence on the surface plasmon characteristics of the nanoparticles was ***:Ag colloidal suspensions were prepared by mixing highly monodisperse Au and Ag *** plasmon band of these mixtures was found to be highly sensitive to Au:Ag concentration ratio and wavelength of the laser beam used in the ablation *** Au:Ag mixture consists of almost spherical shaped nanostructures with a tendency to join with adjacent *** surface enhanced Raman scattering activity of the Au,Ag and Au:Ag colloidal suspensions was tested using crystal violet as probe *** in Raman signal obtained with Au:Ag substrates was found to be promising and strongly depends on its plasmon characteristics.

关键词： Laser ablation Au and Ag nanoparticles Nanocolloids Plasmon SERS Crystal violet