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A 3D Human Liver Model of Nonalcoholic Steatohepatitis

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Journal of Clinical and Translational Hepatology 2020年第4期8卷 359-370页

作者： Marion Duriez Agnes Jacquet Lucile Hoet Sandrine Roche Marie-Dominique Bock Corinne Rocher Gilles Haussy Xavier Vigé Zsolt Bocskei Tamara Slavnic Valérie Martin Jean-Claude Guillemot Michel Didier Aimo Kannt Cécile Orsini vincent Mikol Anne-Céline le Fèvre Translational Sciences Sanofi1 Avenue Pierre BrossoletteChilly-MazarinFrance IT&M STATS Groupe IT&MNeuilly-sur-SeineFrance Non Clinical Biostatistics SanofiVitry sur SeineFrance Diabetes and Cardiovascular Research SanofiIndustriepark HöchstFrankfurtGermany Institute of Experimental Pharmacology Medical Faculty MannheimUniversity of HeidelbergMannheimGermany

Background and Aims: To better understand nonalcoholic steatohepatitis (NASH) disease progression and to evaluate drug targets and compound activity, we undertook the devel-opment of an in vitro 3D model to mimic liver architecture and the NASH environment. Methods:We have developed an in vitro preclinical 3D NASH model by coculturing primary hu-man hepatocytes, human stellate cells, liver endothelial cells and Kupffer cells embedded in a hydrogel of rat collagen on a 96-well plate. A NASH-like environment was induced by ad-dition of medium containing free fatty acids and tumor ne-crosis factor-α. This model was then characterized by biochemical, imaging and transcriptomics analyses. Results:We succeeded in defining suitable culture conditions to main-tain the 3D coculture for up to 10 days in vitro, with the lowest level of steatosis and reproducible low level of inflammation and fibrosis. NASH disease was induced with a custom me-dium mimicking NASH features. The cell model exhibited the key NASH disease phenotypes of hepatocyte injury, steatosis, inflammation, and fibrosis. Hepatocyte injury was highlighted by a decrease of CYP3A4 expression and activity, without loss of viability up to day 10. Moreover, the model was able to stimulate a stable inflammatory and early fibrotic environ-ment, with expression and secretion of several cytokines. A global gene expression analysis confirmed the NASH induc-tion. Conclusions:This is a new in vitro model of NASH dis-ease consisting of four human primary cell-types that exhibits most features of the disease. The 10-day cell viability and cost effectiveness of the model make it suitable for medium throughput drug screening and provide attractive avenues to better understand disease physiology and to identify and characterize new drug targets.

关键词： 3D liver model Human primary cells Key features of NASH

Effect of Zeolite on Small Intestine Microbiota of Broiler Chickens:A Case Study

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Food and Nutrition Sciences 2017年第1期8卷 163-188页

作者： Sandrine le Gall-David vincent Meuric Gaelle Benzoni Sophie Valière Alain Guyonvarch Jacques Minet Martine Bonnaure-Mallet Microbiology Team EA 1254 Universitéde Rennes 1RennesFrance Hospital RennesFrance Invivo NSA Saint NolffFrance INRA UAR 1209Castanet-TolosanFrance INRA GeT-PlaGeGenotoulCastanet-TolosanFrance CNRS-IGDR-UMR 6290 RennesFrance AMADEUS Platform-Biosit RennesFrance

After ban of antibiotics growth promoters (AGP) in Europe in 2006, use of non-medicated gut microbiota regulators as feed additives has dramatically increased. This study aimed at describing the effects of a copper-exchanged zeolite on broiler growth performance, small intestine morphology and microbiota composition. Illumina Sequencing of the V3-V4 region of the 16S rRNA gene was employed to study the small intestine microbiota. This microbiota with copper-exchanged zeolite treated-chickens was significantly less diverse with an almost exclusive presence of Lactobacillus johnsonii and Lactobacillus reuteri. These Lactobacilli are correlated with increased diameter, length and weight of the three segments of the small intestine and decreased viscosity of the intestinal content, suggesting probiotic action. The tested copper-exchanged zeolite would act as a prebiotic, selecting a “favorable” flora for the healthy broilers development.

关键词： Microbiota Chicken Growth Promoting Zeolite Bacitracin

A CYP450X Catalyzing the ω-Hydroxylation of Fatty Acids is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice

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A CYP450X Catalyzing the ω-Hydroxylation of Fatty Acids is ...

Colon cancer stemness as a reversible epigenetic state:Implications for anticancer therapies

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： vincent Sauveplane Franck Pinot Danièle Werck-Reichhart Patrik Diehl Lukas Schreiber Rochus Franke Institut de Biologie Moléculaire des Plantes CNRS UPR2357-Université de Strasbourg67083 Strasbourg Cedex France Institute of Cellular and Molecular Botany University of Bonn D-53115 Bonn Germany

＜正＞The anther cuticle and microspore exine act as protective barriers for male gametophyte and pollen grain, but relatively little is known about the mechanism underlying the biosynthesis of their monomers. We report

关键词： CYP450 fatty acid hydroxylase pollen exine cutin synthesis anther rice

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World Journal of Stem Cells 2019年第11期11卷 920-936页

作者： Audrey vincent Aicha Ouelkdite-Oumouchal Mouloud Souidi Julie leclerc Bernadette Neve Isabelle Van Seuningen Lille University Institut National de la Sante et de la Recherche MédicaleCHU LilleUMR-S 1172-Jean-Pierre Aubert Research CenterLille F-59000France Department of Biochemistry and Molecular Biology Lille University HospitalLille F-59000France

The recent discovery of cancer cell plasticity,*** ability to reprogram into cancer stem cells(CSCs)either naturally or under chemotherapy and/or radiotherapy,has changed,once again,the way we consider cancer *** cancer stemness is a reversible epigenetic state rather than a genetic identity,opportunities will arise for therapeutic strategies that remodel epigenetic landscapes of ***,the systematic use of DNA methyltransferase and histone deacetylase inhibitors,alone or in combination,in advanced solid tumors including colorectal cancers,regardless of their molecular subtypes,does not seem to be the best *** this review,we first summarize the knowledge researchers have gathered on the epigenetic signatures of CSCs with the difficulty of isolating rare populations of *** raise questions about the relevant use of currently available epigenetic inhibitors(epidrugs)while the expression of numerous cancer stem cell markers are often repressed by epigenetic *** markers include the three cluster of differentiation CD133,CD44 and CD166 that have been extensively used for the isolation of colon ***,we describe current treatment strategies using epidrugs,and we hypothesize that,using correlation tools comparing associations of relevant CSC markers with chromatin modifier expression,we could identify better candidates for epienzyme targeting.

关键词： Cancer stem cells Colon cancer Epigenetics Chromatin modifying enzymes CD44 CD133 CD166

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A Suitable Partner

ChinAfrica 2022年第8期14卷 12-13页

作者： vincent MBONIHANKUYE le Renouveau du Burundi Journalism and Communication at Huazhong University of Science and Technology in Wuhan Hubei Province

Cooperation with China is the best way for Africa to achieve sustainable development Since my arrival in China in 2018,I have been fascinated by the Chinese people’s devotion to their jobs and efforts to develop their *** I’ve seen,heard,or read has led me to feel that China is a successful country with which other countries may partner to promote their own *** rapidly developing,inventive country is home to worldclass educational institutions.

关键词： partner institutions Africa

Artificial neural network approach for multiphase segmentation of battery electrode nano-CT images

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npj Computational Materials 2022年第1期8卷 255-265页

作者： Zeliang Su Etienne Decencière Tuan-Tu Nguyen Kaoutar El-Amiry vincent De Andrade Alejandro A.Franco Arnaud Demortière Laboratoire de Réactivitéet Chimie des Solides(LRCS) CNRS UMR 7314Universitéde Picardie Jules VerneHub de l’EnergieRue Baudelocque80039Amiens CedexFrance Réseau sur le Stockage Electrochimique de l’Energie(RS2E) CNRS FR 3459Hub de l’EnergieRue Baudelocque80039Amiens CedexFrance MINES ParisTech–PSL Research University CMMCenter for Mathematical Morphology35 rue Saint HonoréFontainebleauFrance Renault Technocentre 78084GuyancourtFrance Advanced Photon Source Argonne National LaboratoryLemontUSA Institut Universitaire de France 101 Boulevard Saint Michel75005ParisFrance ALISTORE-European Research Institute CNRS FR 3104Hub de l’EnergieRue Baudelocque80039Amiens CedexFrance

The segmentation of tomographic images of the battery electrode is a crucial processing step,which will have an additional impact on the results of material characterization and electrochemical ***,manually labeling X-ray CT images(XCT)is time-consuming,and these XCT images are generally difficult to segment with histographical *** propose a deep learning approach with an asymmetrical depth encode-decoder convolutional neural network(CNN)for real-world battery material *** network achieves high accuracy while requiring small amounts of labeled data and predicts a volume of billions voxel within few *** applying supervised machine learning for segmenting real-world data,the ground truth is often *** results of segmentation are usually qualitatively justified by visual *** try to unravel this fuzzy definition of segmentation quality by identifying the uncertainty due to the human bias diluted in the training *** CNN trainings using synthetic data show quantitative impact of such uncertainty on the determination of material’s ***-XCT datasets of various battery materials have been successfully segmented by training this neural network from *** will also show that applying the transfer learning,which consists of reusing a well-trained network,can improve the accuracy of a similar dataset.

关键词： neural network battery

Commeune grande seur La coopération sino-africaine est celle qui se prete le mieux au developpement durable en Afrique

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中国与非洲（法文版） 2022年第8期14卷 12-13页

作者： vincent MBONIHANKUYE l'Universitedes sciences et technologies du Centre de la Chinea Wuhan au Hubei

Optical characterization of antimony-doped ZnO thin layers grown by MO-VPE

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Optical characterization of antimony-doped ZnO thin layers g...

Kenya's Curling Moment

第六届国际氧化锌及相关材料研讨会

作者： Haneche Nadia Lusson Alain Sartel Corinne Jomard Franois Vilar Christèle Sallet vincent Pierre Galtier Groupe d’Etude de la Matière Condensée （GEMaC） CNRS-Université de Versailles St-Quentin 92195 Meudon cedex France

＜正＞It is well known that p type conductivity is difficult to obtain in ZnO, meanwhile the substitution of oxygen atoms by column V elements is a possible route to get acceptors in ZnO. In this study, we have incorp...

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ChinAfrica 2022年第4期14卷 48-49页

作者： éRIC vincent FOMO 不详

The East African country is a sports powerhouse, especially in athletics. It is now trying to develop curling An athlete moves forward, focused on his aim, and slides a stone across the ice. The curl is in place. Two other athletes use brooms to furiously wipe the ice against the advancing piece of granite to keep it on course, without touching it, so that it comes to a stop close to the center of the “house,” as the target on the track is called. Curling is a high-intensity sport that needs strategy and focus.

关键词： Curling sports piece