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Chemical synthesis of a structural gene coding for trichosanthin

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Chinese Journal of Chemistry 1995年第4期13卷 349-357页

作者： CHEN, HB XIA, Y JING, jp JIANG, K BAO, JS CHINESE ACAD SCI SHANGHAI INST ORGAN CHEMCOMP CHEM LABSTATE KEY LAB BIOORGAN & NAT PROD CHEMSHANGHAI 200032PEOPLES R CHINA

A structural gene (750 bp), which codes for a type I ribosome inactivating protein, trichosanthin, has been designed according to the codon usage of highly expressed gene in E. coli and chemically synthesized. In the synthesized gene, twenty-seven unique restriction sites were evenly dispersed with an average distance between two adjacent sites less than 50 bp to facilitate a systematic investigation on structure-functional relationship of this protein by site-directed mutagenesis. To synthesize it, the whole gene was divided into three large fragments (EP, PN and NH) which were assembled from several chemical synthetic oligonucleotides by enzymatic method. The assembly of both the fragment EP from six oligonucleotides (A-F) and the fragment PN from four oligomers (G-J) was catalyzed by T-4 DNA ligase in using the single stranded DNA method [Chen, H.-B. et al., Nucl. Acids Res., 18, 871(1990)]. And fragment NH was formed from three duplexes K, l and M by the classical double stranded DNA method. Finally, each fragment was cloned into vector pUC18 in succession to form the plasmid, pC0TCS, to complete the whole gene synthesis, The sequencing data for the synthetic gene coincides with the designed one.

关键词： RIBOSOME INACTIVATING PROTEIN TRICHOSANTHIN GENE DESIGN GENE SYNTHESIS SYNTHETIC GENE

Electrochemical Studies on the Deposition of Neodymium in DMSO

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Chinese Chemical letters 1998年第2期9卷 169-171页

作者： liu, GK Gu, lW Tong, YX liu, jp lu, SJ Zhongshan Univ Sch Chem & Chem Engn Guangzhou 510275 Peoples R China

The electrodeposition of neodymium on Pt and Cu electrodes was investigated in DMSO containing NdCl3 and a small mount of water at room temperature. Cyclic voltammetry indicated that the reduction process of Nd(III) on the Pt cathode was complicated. The one-step reduction wave showed two steps by adding 0.3 mol/l ethylenediamine(EN). A delicate yellow, lustrous, strongly adhering film on the Cu electrode was obtained using potentiostatic electrolysis at -1.80 V (vs. aq SCE).

关键词： electrochemical studies deposition neodymium

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治疗血栓形成性疾病的新观点

国外医学（输血及血液学分册） 1990年第4期13卷 225-226页

作者： Drouet l Caen jp 羊裔明

预防血栓形成必须把主要因素或那些依赖病理条件改变了局部血流特点的因素作为目标.据此,治疗的目的可以是:1.抑制血小板的功能;2.限制凝血因子;3.刺激血管内皮抗血栓性能,主要是促纤溶特性;4.改善血流条件.抗血小板药物可按照抗血小板... 详细信息

预防血栓形成必须把主要因素或那些依赖病理条件改变了局部血流特点的因素作为目标.据此,治疗的目的可以是:1.抑制血小板的功能;2.限制凝血因子;3.刺激血管内皮抗血栓性能,主要是促纤溶特性;4.改善血流条件.抗血小板药物可按照抗血小板药物作用的部位分类:1. 作用膜受体的药物血小板浆膜受体有好几个类型,血小板聚集和活化的特异性激动剂,如ADP、TXA_2、血小板活化因子Acether(AGPEC)、凝血酶、肾上腺素和5-羟色胺已被加以描述.

关键词：血栓形成疾病

Machinable REPM with Excellent Intrinsic Coercive ForcePrepared by DS Technology

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Journal of Materials Science & Technology 1997年第6期13卷 475-478页

作者： liu, XC Pan, J Xu, ZF Zhuang, jp liu, M Yu, DM Shi, ZX Fu, HZ Nanchang Inst Aeronaut Technol Nanchang 330034 Peoples R China Northwestern Polytech Univ Xian 710072 Peoples R China

To obtain excellent magnetic properties of machinable REPM prepared by directional solidification (DS) technology, Space A, a composition area with theta=0 degrees similar to 5 degrees where easy axis is parallel to solidified direction of alloys, has been established in Zr-Sm-la-Co-Cu-Fe system. In 2/17+1/5+Co, and 2/17+1/5 region near Co phase of Space A, one obtains: V-f = 80.933 - 2.739Zr - 1.828Sm - 0.773Co - 0.147Cu + 0.415Fe + 0.218la Nonequilibrium solidification process of alloys in 2/17+1/5+Co region, is as follows: l-->Co, l-->2/17, l+2/17-->1/5 and l-->1/(5-x)+1/(5+x). The product of partially completed peritectic reaction, and 1/(5-x) and 1/(5+x) phases can all be transformed into a homogeneous 1/5 phase after solution treatment in 1413 K for 5 h. Containing 3 similar to 6% Co phase magnetically hardened by 0.5 similar to 2.5% V, the DS magnets have been achieved with both improved toughness and excellent coercive force of 520 kA/m.

关键词： Permanent magnets

Viscoelastic Properties and Bioactivity of Sol-Gel Derived Gelatin-Silicate Composites: Effects of the Incorporated Ca^(2+) Ions

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Journal of Materials Science & Technology 2001年第3期17卷 367-370页

作者： l.Ren, K.Tsuru, S.Hayakawa and A.Osaka Biomaterial laboratory, Faculty of Engineering, Okayama University, Tsushima 3-1-1, Okayama-shi 700-8530, Japan E-mail: osaka@cc.okayama-u.ac.jp Faculty of Eng. Okayama Univ. Tsushima 3-1-1 Okayama-shi 700-8530 Japan

Several gelatin-silicate composites, with or without incorporation of Ca2+ ions, were synthesized through sol-gel processing starting from gelatin and 3- (glycidoxypropyl) trimethoxysilane. The structure around the Si atoms was similar for all the samples. The measurement of viscoelastic properties indicated that the glass transition temperature and activation energy decreased with the incorporation of Ca2+ ions. The Ca2+ ion-containing composites were bioactive as they spontaneously deposited apatite when soaked in a simulated body fluid of the Kokubo recipe.

关键词： Si Ions Viscoelastic Properties and Bioactivity of Sol-Gel Derived Gelatin-Silicate Composites Effects of the Incorporated Ca

Antibacterial activity of sequentially extracted organic solvent extracts of fruits,flowers and leaves of lawsonia inermis *** Jaffna

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 798-802页

作者： E Christy Jeyaseelan S Jenothiny MK Pathmanathan jp Jeyadevan Department of Botany Faculty of ScienceUniversity of Jaffna Department of Chemistry Faculty of ScienceUniversity of Jaffna

Objective:To reveal the antibacterial activity of sequentially extracted different cold organic solvent extracts of fruits,flowers and leaves of lawsonia inermis(l against)some pathogenic ***:Powders of fruits,flowers and leaves of l inermis were continuously extracted with dichloromethane(DCM),ethyl acetate and ethanol at ambient *** dried extracts were prepared into different concentrations and tested for antibacterial activity by agar well diffusion method,and also the extracts were tested to detennine the available ***:Except DCM extract of flower all other test extracts revealed inhibitory effect on all tested bacteria and their inhibitory effect differed significantly(Pl acetate extract of flower against Staphylococcus aureus(***)and Pseudomonas aeruginosa(***),and ethyl acetate extract of fruit on Escherichia coli(***)and Bacillus subtilis(***).The ethyl acetate and ethanol extracts of flower,fruit and leaf expressed inhibition even at 1 mg/100μl against all test *** the tested phytochemicals flavonoids were detected in all test extracts except DCM extract of ***:The study demonstrated that the ethyl acetate and ethanol extracts of fruit and flower of l inermis are potentially better source of antibacterial agents compared to leaf extracts of respective solvents.

关键词： lawsonia inermis Antibacterial activity Sequential extraction Well diffusion method

Surgery for pancreatic necrosis:“Whom, when and what”

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World Journal of Gastroenterology 2004年第12期10卷 1697-1698页

作者： S Connor jp Neoptolemos Department of Surgery Royal Liverpool University HospitalDaulby StreetLiverpoolL69 3GAUK

Acute pancreatitis is a common condition in which 70% of patients will recover with simple medical management. For patients who develop extensive or infected pancreatic necrosis the outcome is significantly different with a high morbidity and mortality. Surgery is the mainstay of treatment for these patients but several unresolved issues remain including who requires surgery, when is the optimal time to intervene and what technique should be used.

关键词：外科手术胰腺坏疽死亡率介入技术

相转移催化环加成反应合成1-芳磺酰基-2-苯甲酰基环丙烷系列化合物(Ⅰ)

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化学试剂 1996年第5期18卷 263-265,269页

作者： Idoux,jp 刘长畿中国科学院上海有机化学研究所

苯甲酰硫叶立德对芳磺酰基乙烯的环加成合成了标题系列化合物，它们是一类生物活性物质。

关键词：相转移催化环加成生物活性环丙烷

重组内皮抑制素抑制大鼠角膜移植术后的新生血管

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中国组织工程研究与临床康复 2007年第51期11卷 10221-10225页

作者：程琳周善璧步建平重庆医科大学附属第一医院眼科重庆市400016

目的:内皮抑制素是一种内源性血管内皮细胞的强效抑制剂,实验拟验证局部应用重组内皮抑制素滴眼液对大鼠角膜移植术后新生血管的抑制作用。方法:实验于2005-09/2006-03在重庆医科大学附属第一医院实验动物中心完成。①选用清洁级成年雌... 详细信息

目的:内皮抑制素是一种内源性血管内皮细胞的强效抑制剂,实验拟验证局部应用重组内皮抑制素滴眼液对大鼠角膜移植术后新生血管的抑制作用。方法:实验于2005-09/2006-03在重庆医科大学附属第一医院实验动物中心完成。①选用清洁级成年雌性SD大鼠(受体)60只,Wistar大鼠(供体)30只,建立大鼠同种异体穿透性角膜移植模型,供体提供双眼角膜,受体均选择右眼手术。移植术后按随机数字表法分成内皮抑制素组和对照组,分别用50mg/l的内皮抑制素眼液及赋形剂滴眼。②于术后1周、2周、3周、1个月及2个月5个时间点,麻醉后每组随机处死6只大鼠。观察角膜新生血管生长状况;比较各组角膜植片透明度、水肿、新生血管程度及移植排斥反应指数;检测角膜植片上内皮抑制素及血管内皮生长因子的表达情况。结果:60只受体大鼠全部进入结果分析。①内皮抑制素组大鼠角膜新生血管面积显著低于对照组(P<0.05￣0.01)。②内皮抑制素组大鼠的排斥反应指数在术后1周、2周、3周、1个月及2个月均低于对照组(P<0.05￣0.01)。③与对照组相比,内皮抑制素组角膜植片组织中内皮抑制素的表达明显增强(P<0.05￣0.01),血管内皮生长因子的表达明显降低(P<0.05￣0.01)。结论:内皮抑制素滴眼液可以有效抑制大鼠角膜移植术后角膜新生血管的形成,下调角膜植片中血管内皮生长因子的表达,从而降低角膜移植术后免疫排斥反应的发生,为临床防治角膜移植术后新生血管提供了新的思路。

关键词：角膜移植内皮抑制素角膜新生血管化内皮生长因子组织移植