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Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i>and Its Immunogenic Potential

Advances in Bioscience and Biotechnology 2015年第7期6卷 443-451页

作者： Sunita Kumari Yadav Carmelita N. Marbaniang vibhuti sharma Aparna Dixit School of Biotechnology Jawaharlal Nehru University New Delhi India RNA Biology Group Institute for Molecular Infection Biology University of Würzburg Würzburg Germany

Aeromonas hydrophila, a gram negative bacterium is a major fish pathogen and causes major economic losses to aquaculture industry. Outer membrane proteins play a significant role in its survival during different environmental conditions and bacterial pathogenesis. The outer membrane protein R (OmpR) is a member of the two-component regulatory system of Aeromonas hydrophila which differentially regulates the expression of OmpF or OmpC depending on the osmolarity conditions. Role of OmpR has been demonstrated in its virulence in other infectious bacteria and it is found to be a potential drug target/vaccine candidate. However, the OmpR of A. hydrophila has not been characterized. In the present study, we report recombinant expression, purification of the OmpR of A. hydrophila strain Ah17 in salt inducible E. coli GJ1158 cells. Leaky expression of rOmpR was confirmed by Western blot analysis using anti-6 × His antibody. The histidine tagged recombinant OmpR (rOmpR) (~29 kDa) was purified using Ni-NTA affinity chromatography from the soluble fraction of induced E. coli cells. The rOmpR was found to be highly immunogenic with end point titres of greater than 1:80,000. The anti-rOmpR antisera were capable of agglutinating live A. hydrophila cells, thus showing vaccine potential of the rOmpR.

关键词： OmpR Aeromonas Cross-Reactivity Outer Membrane Protein Expression E. coli GJ1158

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Smartphone dependency and its impact on academics among medical and nursing students: A cross-sectional study

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Journal of Integrative Nursing 2022年第1期4卷 30-35页

作者： Rakesh sharma vibhuti VAIDYA Rincy RAJAN Anumol Thottiyil ELDHOSE Hemkala RATRE Hemant Lata RAI Department of Neurosciences Nursing College of NursingAll India Institute of Medical SciencesRishikeshUttarakhandIndia Department of Pediatrics All India Institute of Medical SciencesRaipurChhattisgarhIndia Department of Emergency All India Institute of Medical SciencesRaipurChhattisgarhIndia Obstetrics and Gynecology Nursing Government College of NursingRaipurChhattisgarhIndia

Objective:This study aimed to assess smartphone dependency and its impact on academics among medical and nursing *** and Methods:A cross-sectional study was carried out on Bachelor of Medicine and Bachelor of Surgery(MBBS)and Bachelor of Science in Nursing(BScN)students in a medical teaching *** Smartphone Dependency Scale and self-structured questionnaire on impact of smartphone on academics were used to assess smartphone dependency and its impact on academics.A total of 436 students were selected using the total enumerative sampling *** were analyzed using the descriptive(frequency,percentage,mean,and standard deviation)and inferential(t-test,Chi-square test)***:The mean age of students was 20.6±1.29 years,81%were females,and the mean body mass index score was 21.59±3.41 kg/*** mean impact on academics and smartphone dependency scores was 19.92±7.01 and 48.58±11.46,*** impact on academics had a significant association with student category(P<0.001)and gender(P<0.001).A significant association was found between the impact on academics(P=0.003)and smartphone dependency(P=0.05)with studying ***:The use of smartphones is more among medical *** studying in the first and second years are found to be more dependent on smartphone,which caused a serious impact on their *** appliances have become mandatory in this era of technology,and it is not possible to stop its usage but negative impact of smartphones on students’academic performance needs to be ***,it is mandatory to organize educational seminars and workshops to promote the appropriate use of smartphones.

关键词： Internet addiction disorder medical nursing smartphone students

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印度外包企业大打家庭牌

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中国计算机用户 2008年第21期 26-27页

作者： Amol sharma Jackie Range vibhuti Agarwal

十个月前，当外包企业Keane Inc.聘用Jyoti Taneja在位于印度Gurgaon的办公室工作后，Taneja的父母都感到非常担心。22岁的Taneja女士负责接听美国医疗保险公司的客户来电，她每天要到凌晨2：30才能下班。

关键词：企业外包印度家庭办公室工作保险公司

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