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The non-coding RNA SVALKA locus produces a cis-natural antisense transcript that negatively regulates the expression of CBF1 and biomass production at normal temperatures

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Plant Communications 2023年第4期4卷 77-87页

作者： vasiliki zacharaki Shiv Kumar Meena Peter Kindgren UmeåPlant Science Centre Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural Sciences90187 Umea°Sweden

Non-coding transcription is present in all eukaryotic genomes,but we lack fundamental knowledge about its importance for an organism’s ability to develop *** plants,emerging evidence highlights the essential biological role of non-coding transcription in the regulation of coding ***,we have few molecular insights into this ***,we show that a long isoform of the long noncoding RNA SVALKA-L(SVK-L)forms a natural antisense transcript to the host gene CBF1 and negatively regulates CBF1 mRNA levels at normal temperatures in the model plant Arabidopsis ***,we show detailed evidence for the specific mode of action of *** pathway includes the formation of double-stranded RNA that is recognized by the DICER proteins and subsequent downregulation of CBF1 mRNA ***,the CBF1-SVK regulatory circuit is not only important for its previously known role in cold temperature acclimation but also for biomass production at normal *** study characterizes the developmental role of SVK-L and offers mechanistic insight into how biologically important overlapping natural antisense transcripts can act on and fine-tune the steady-state levels of their host gene’s mRNA.

关键词： SVALKA cis-natural antisense transcript non-coding transcription CBF1 Arabidopsis

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Education on Sustainable Development based on Local Agenda 21

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Journal of Environmental Protection 2011年第4期2卷 371-378页

作者： Constantina Skanavis Polytimi zacharaki Christos Giannoulis vasiliki Petreniti Department of Environment University of the Aegean Greece Department of Environment University of the Aegean Greece.

The study is contextualized through evaluating the implementation of Local Agenda Action Plan (LAAP). The goal was to address environmental challenges facing a city under the framework of sustainable development and planning. The evaluation of this conceptual framework was tested at a selected municipality of Athens (Greece), that has used a LAAP. The research was based on a structured questionnaire survey of a sample of 300 respondents selected from municipal employees (100 persons) and citizens of the selected municipality (200 persons). Analysis and interpretation of data through descriptive and factor analysis statistics establish that both municipality employees and citizens are primarily concerned with issues of environmental management. They firmly believe that sustainability initiatives at municipality might best be implemented through a collaborative approach at the local community level, involving local citizens working in partnership with local government. Furthermore, the study established the importance of education, aware-ness and training as a response to environmental issues currently facing the municipality. The awareness and training activities should be developed and should involve the members of the community in the needed environmental manage-ment processes. In view of the Decade Education for Sustainable Development (UNESCO 2005-2015), this should en-able the Council to create opportunities for income generation, while simultaneously promoting citizens’ environmental responsible behaviors and improving service delivery by municipality employees.

关键词： Environmental Policy Local Agenda 21 Education Sustainable Development Greece Evaluation

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