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Detection of Gluten in Dishes Offered as Gluten-Free in a Gastronomic Area Located in San José, Costa Rica

Natural Science 2022年第12期14卷 524-531页

作者： vanessa bagnarello-madrigal Daniela Rodríguez-Chaves Mayra Villegas-Barakat Andrés Chacón-Robles Moisés Coto-Hernández Escuela de Fisioterapia Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Escuela de Nutrición Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Facultad de Microbiología Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Unidad del Aseguramiento de la Calidad Universidad de Ciencias Médicas (UCIMED) San José Costa Rica

Celiac disease, gluten sensitivity and gluten intolerance are health conditions that require consumers to avoid their gluten intake. In Costa Rica, Law 8975 regulates the amount of gluten permitted in prepackaged products labeled as “gluten-free”, but the parameters to be followed are not mandated for food service operations. This study investigated whether restaurants with gluten-free (GF) options in a gastronomic area of the city of San José, Costa Rica, achieved the requirements of the legislation that the dishes offered should contain less than 20 ppm gluten. Using data collected from five restaurants offering GF dishes, two provided dishes with quantities of gluten greater than 20 ppm (restaurant A, four samples and restaurant B, three samples);particularly dishes from the category “meat with sauce”. Although those dishes are naturally gluten-free, when they are handled in areas of shared production, they are exposed to the risk of cross contact, furthermore the adding of misscellaneous such as: marinades, sauces and condiments, which could contain traces of gluten or hidden gluten, a risk associated with the use of ingredients without certification GF (supplier’s practices and label declaration). Therefore improvements in food service procedures should be enforced. The findings of the present study emphasize the need to include restaurant foods in the relevant legislation to ensure that the gluten-intolerant sector of the population remains safe when eating out.

关键词： Celiac Disease Gluten-Free (GF) Gliadin Shared Production Cross Contact

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Antimalarial Activity of an Alkaloid Mixture Isolated from the Tender Leaves of the Nectandra membranacea （Laureaceae）

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Journal of Life Sciences 2018年第3期12卷 134-140页

作者： vanessa bagnarello-madrigal Daniela Rodríguez-Chaves Javier Alpizar-Cordero Alonso Calvo-Vargas Maribel Cordero-Villalobos Misael Chinchilla-Carmona Idalia Valerio-Campos Ronald Sánchez -Porras Departamento de Química Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Departamento de Investigación Básica Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Sección de Biología Sede Occidente Universidad de Costa Rica San José 10108 Costa Rica

Despite the breadth of knowledge about malaria, its transmission, preventive measures and available treatments and its difficulty in controlling the vector and the rapid spread of resistance to current drugs, there is a stimulus to develop new research looking for active molecules. The phytochemical studies of the active extract confirmed the presence of alkaloids, flavonoids, coumarins, tannins, reducing compounds, anthocyanins, terpenes, triterpenes and steroids. In the present work, it was possible to isolate a mixture of isoquinolinic alkaloids from the tender leaves of Nectandra membranacea （Laureaceae） with antimalarial activity. The mixture composed of （-） - gliazovine and （＋） - aplogliazovine showed an IC50 value of 32.18 μg/mL against the P. berghei NK65 strain. It is important to continue with these studies and their chemical components isolated from N. membranacea that could be used as an alternative the treatment of malaria.

关键词： Malaria Nectandra membranacea alkaloids phytochemical screening Laureaceae.

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Detection of Gluten in Dishes Offered as Gluten-Free in a Gastronomic Area Located in San José, Costa Rica

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Natural Science（自然科学期刊） 2022年第12期

作者： vanessa bagnarello-madrigal Daniela Rodríguez-Chaves Mayra Villegas-Barakat AndrésChacón-Robles Moisés Coto-Hernández

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