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Advances in small animal mesentery models for in vivo flow cytometry,dynamic microscopy,and drug screening

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World Journal of Gastroenterology 2007年第2期13卷 192-218页

作者： Ekaterina I Galanzha valery v tuchin vladimir P Zharov Philips Classic Laser Laboratories University of Arkansas for Medical Sciences (UAMS) Little Rock AR United States Department of Optics and Biomedical Physics Research-Educational Institute of Optics and Biophotonics Saratov State University Saratov Russia Department of Optics and Biomedical Physics Research-Educational Institute of Optics and Biophotonics Saratov State University Saratov Russia Philips Classic Laser Laboratories University of Arkansas for Medical Sciences (UAMS) Little Rock AR United States

Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo. Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell’s functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed.

关键词： Lymph microcirculation Transmission digital microscopy Rat mesentery Flow cytometry Photothermal technique

Shedding light on biology and healthcare--preface to the special issue on Biomedical Optics

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Light(Science & Applications) 2022年第7期11卷 1315-1317页

作者： Peng Xi Xunbin Wei Junle Qu valery v.tuchin Department of Biomedical Engineering College of Future TechnologyPeking University100871BeijingChina Department of Biomedical Engineering Peking University100081BeijingChina Center for Biomedical Optics and Photonics(CBOP)&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of EducationShenzhen University518060ShenzhenChina Saratov State University 83 Astrakhanskaya str.Saratov410012Russia

During the past decade,there has been an explosion in the development of novel optical techniques in biomedical research and clinical *** progress has been made in optical imaging and detection to provide necessary biological information for disease diagnosis and in-depth understanding of biological ***,recent advances integrating laser technology with biomarkers have enabled novel diagnostic and treatment approaches for different types of diseases.

关键词： optical integrating diagnosis

INTRODUCTION:SPECIAL ISSUE ON ADvANCES IN BIOPHOTONICS AND BIOMEDICAL OPTICS-PART I

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Journal of Innovative Optical Health Sciences 2013年第2期6卷 16-17页

作者： Dan Zhu valery v.tuchin Qingming Luo Huazhong University of Science and Technology WuhanP.R.China Saratov State University SaratovRussia

The 5th Russian-Chinese Workshop on Biophotonics and Biomedical Optics was hosted in Saratov,Russia on 26-28,September,*** bilateral Workshop brought together both Russian and Chinese scientists,engineers and clinical researchers from a variety of disciplines engaged in applying optical science,photonics and imaging technologies to problems in biology and *** the W orkshop,2 plenary lectures,11 invited presentations,4 oral presentations and 13 poster reports were presented.A special Internet session with 5 presentations-1 plenary,2 invited,and 2 posters was also *** special issue selects some papers from the attendees,and is made up of 12 original research articles and one *** articles will be presented in vol.6 No.1 of JIOHS,while the remaining five articles will be presented in vol.6 No.2.

关键词： Russian photonics engineer

OPTICAL PROPERTIES OF SKIN,SUBCUTANEOUS,AND MUSCLE TISSUES:A REvIEW

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Journal of Innovative Optical Health Sciences 2011年第1期4卷 9-38页

作者： ALEXEY N.BASHKATOv ELINA A.GENINA valery v.tuchin Institute of Optics and Biophotonics Saratov State University Saratov 410012Russia Institute of Precise Mechanics and Control of RAS Saratov 410028Russia

The development of optical methods in modern medicine in the areas of diagnostics,therapy,and surgery has stimulated the investigation of optical properties of various biological tissues,since the efficacy of laser treatment depends on the photon propagation and fluence rate distribution within irradiated *** this work,an overview of published absorption and scattering properties of skin and subcutaneous tissues measured in wide wavelength range is *** principles of measurements of the tissue optical properties and techniques used for processing of the measured data are outlined.

关键词： Absorption coefficient scattering coefficient anisotropy factor reduced scattering coefficient refractive index

Foreword to the Special Issue on Optical Technologies in Biophysics and Medicine

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Journal of Innovative Optical Health Sciences 2015年第3期8卷 1-2页

作者： Elina A.Genina Dan Zhu valery v.tuchin Research-Educational Institute of Optics and Biophotonics Saratov State University SaratovRussia Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology(HUST)Wuhan P.R.China Research-Educational Institute of Optics and Biophotonics Saratov State University Institute of Precise Mechanics and Control RAS Saratov Russia

We are pleased to present the third issue of JIOHS,which focuses on optical technologies of diagnosticsand therapy,These are selected papers presented during Saratov Fall Meeting,1t International Sym-posium on Optics and Biophotonics(September 24-28,2013,Saratov,Russia)in the framework of the Workshops:"Optical Technologies in Biophysics_and Medicine,"Nanobiophotonics,"and"Internet Biophotonics."

关键词： Biophys ics photonics

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FOREWORD

Journal of Innovative Optical Health Sciences 2009年第1期2卷 I0001-I0002页

作者： Elina A.Genina Kirill v.Larin valery v.tuchin Research-Educational Institute of Optics and Biophotonics Saratov State University Saratov Russia Department of Biomedical Engineering University of Houston Houston USA Research-Educational Institute of Optics and Biophotonics Saratov State University Institute of Precise Mechanics and Control RAS Saratov Russia

Special Issue Optical Technologies in the Study of Tissues and Biological Fluids We are pleased to present this special issue of JIOHS,which focuses on optical technologies in the study of tissues and biological *** selected papers were presented during Saratov Fall Meeting—XII International School for Junior Scientists and Students on Optics,Laser Physics and Biophotonics(September 23–26,2008,Saratov,Russia)in the framework of the following workshops:“Optical Technologies in Biophysics and Medicine,”“Nanostructures and Nanoparticles,”“Microscopic and Low-Coherence Methods in Biomedical Applications,”and“Internet Biophotonics.”All eight papers in this special issue are research papers.

关键词： Junior photonics Russia

Introduction to the Special Issue on Advances in Biophotonics and Biomedical Optics

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Journal of Innovative Optical Health Sciences 2021年第5期4卷 1-2页

作者： Polina Dyachenko(Timoshina) Tingting Yu Dan Zhu valery v.tuchin Research-Educational Institute of Optics and Biophotonics Saratov State University Saratov 410012Russia Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China MoE Key Laboratory for Biomedical Photonics School of Engineering Sciences Huazhong University of Science and Technology Wuhan 430074 P.R.China

The 9th Chinese-Russian Workshop on Biopho-tonics and Biomedical Optics was held online on 28-30 September *** bilateral workshop brought together both Russian and Chinese scientists,engineers,and clinical researchers from a variety of disciplines engaged in applying optical science,photonics,and imaging technologies to problems in biology and *** the workshop,2 ple-nary lectures,35 invited presentations,5 oral pre-sentations,and 8 internet reports were *** special issue selects some papers from the attendees and includes both research and review articles.

关键词： photonics Russian Biomedical

Optical clearing mechanisms characterization in muscle

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 82-100页

作者： Luís Oliveira M.Inês Carvalho Elisabete Nogueira valery v.tuchin Physics Department–Polytechnic Institute of Porto School of Engineering Rua Dr.Antonio Bernardino de Almeida 4314200-072 PortoPortugal FEUP–University of Porto Rua Dr.Roberto Frias 4200-465 PortoPortugal CIETI–Centre of Innovation in Engineering and Industrial Technology ISEP Rua Dr.Antonio Bernardino de Almeida4314200-072 PortoPortugal DEEC/FEUP and INESC TEC University of Porto Rua Dr.Roberto Frias 4200-465 PortoPortugal Research-Education Institute of Optics and Biophotonics Saratov National Research State University 83 Astrakhanskaya street Saratov 410012Russia Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision Mechanics and Control RAS 24 Rabochaya street Saratov 410028Russia Interdisciplinary Laboratory of Biophotonics Tomsk National Research State University 36 Lenin's avenue Tomsk 634050Russia

Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological *** applied in cooperation with clinical methods it provides optimization of diagnosis and treatment *** technique turns biological tissues more transparent through two main mechanisms-tissue dehydration and refractive index(RI)matching between tissue *** matching is obtained by partial replacement of interstit ial water by a biocompat ible agent that presents higher RI and it can be completely rever sible by natural rehydration in vrivo or by assisted rehydration in ex vivo *** data to characterize and discriminate between the two mechanisms and to find new ones are *** a simple method,based on collimated transmittance and thickness measurements made from muscle samples under treatment,we have estimated the diffusion properties of glucose,ethylene glycol(EG)and water that were used to perform such char acterization and *** these properties with data from literature that characterize their diffusion in water we have observed that musde cell membrane permeability limits agent and water diffusion in the *** same experi-mental data has allowed to calculate the optical dearing(OC)eficiency and make an inter-pretation of the internal changes that occurred in muscle during the *** same methodology can now be used to perform similar studies with other agents and in other tissues in order to solve engineering problems at design of inexpensive and robust technologies for a *** improvement of optical tomogr aphic techmiques with better contrast and in depth imaging.

关键词： Collimated transmittance agent diffusivity tissue dehydration thickness variation refractive index matching glucose ethylene glycol

Introduction to the Special Issue on Advances in Biophotonics and Biomedical Optics:Part II

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Journal of Innovative Optical Health Sciences 2022年第1期15卷 29-31页

The 9th Chinese–Russian Workshop on Biophotonics and Biomedical Optics was held online on 28–30 September *** bilateral workshop brought together both Russian and Chinese scientists,engineers,and clinical researchers from a variety of disciplines engaged in applying optical science,photonics,and imaging technologies to problems in biology and *** the workshop,2 plenary lectures,35 invited presentations,5 oral presentations,and 8 internet reports were *** special issue selects some papers from the attendees and includes both research and review articles.

关键词： photonics Russian Biomedical