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Effect of the rearing diet on gene expression of antimicrobial peptides in Hermetia illucens(Diptera:Stratiomyidae)

Insect Science 2023年第4期30卷 933-946页

作者： valentina Candian Carlotta Savio Marco Meneguz Laura Gasco Rosemarie Tedeschi Dipartimento di Scienze Agrarie Forestali eAlimentari(DISAFA)University of TorinoGrugliascoItaly NRAE MicalisGMEUniversity of Paris SaclayJouy-en-JosasFrance Laboratory of Entomology Wageningen UniversityWageningenThe Netherlands BEF Biosystems TorinoItaly

Insect proteins have been proposed for human and animal food *** the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to ***,new strategies for preserving insect health must be *** of the insect im-mune system through the diet is one such *** evaluated gene expression of two antimicrobial peptides(one defensin and one cecropin)in Hermetia illucens(L.)(Diptera:Stratiomyidae)reared on different *** were performed on prepupae and 10-day-old larvae reared on cereal-and municipal organic waste-based diets and on only prepupae reared on a cereal-based diet supplemented with sunflower,corn,or soybean *** inclusion of sunflower oil at different points in the cereal-based diet was also ***,diet-driven differences in the inhibitory activity of the hemolymph were tested against Escherichia coli DH5αand Micrococcus yunnanensis HI55 using diffusion assays in solid *** showed that a municipal organic waste-based diet produced a significant overexpression of antimicrobial peptides only in *** of veg-etable oils caused an upregulation of at least one peptide,except for the corn *** expression of both genes was observed when sunflower oil was added 5 days before *** hemolymph samples showed an inhibitory activity against bacteria *** results suggest that municipal organic waste-based diet and vegetable oil-added diet may successfully impact the immune system of *** alternatives may also exist forotherspeciesofeconomic interest.

关键词： cecropin cereal defensin hemolymph organic municipal solid waste vegetable oil

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Molecular survey on zoonotic tick—borne bacteria and chlamydiae in feral pigeons(Columba livia domestica)

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 316-319页

作者： valentina Virginia Ebani Fabrizio Bertelloni Paolo Mani Department of Veterinary Science University of Pisa

Objective;To determine the presence of zoonotic tick-borne bacteria in feral pigeons(Columba lixia domestica) from urban ***:Spleen samples from 84 feral pigeons,found dead with traumatic injuries in urban areas,were examined by PCR to detect DNA of Anaplasma phagocytophilum,Bartonella spp.,Borrelia burgdorferi sensu *** *** spp.,and Chlamydnphila ***:Twenty(23.8%) pigeons were infected by tick-borne agents,in particular 2(2.38%) animals resulted positive for Bartonella spp.,5(5.95%) for Coxiella burnetii.5(5.95%) for Rickettsia spp.,13(15.47%) for Borrelia burgdorferi sensu *** birds scored negative for anaplasma ***,17(20.23%) pigeons were positive for Chlamydophila *** among them 10(11.9%) for Chlamydophila psittaci,Mixed infections by two or three agents were detected in 8(9.52%) ***:Feral pigeons living in urban and periurban areas are a hazard for the human health as source of several *** obtained results confirm pigeons as reservoirs of chlamydial agents and suggest that they may be involved in the epidemiology of zoonotic tick-borne infections too.

关键词： Pigeon(Columba livia domestica) Tick-borne bacteria Chlamydophila psittaci PCR

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Superlubricity of epitaxial monolayer WS2 on graphene

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Nano Research 2018年第11期11卷 5946-5956页

作者： Holger Buch Antonio Rossi Stiven Forti Domenica Convertino valentina Tozzini Camilla Coletti Center for Nanotechnology Innovation @NEST Istituto Italiano di Tecnologia Piazza S. Silvestro 12 56127 Pisa Italy NEST Istituto Nanoscienze - CNR and Scuola Normale Supefiore Piazza San Silvestro 12 56127 Pisa Italy Graphene Labs Istituto Italiano di Tecnologia Via Morego 30 16163 Genova Italy

We report the superlubric sliding of monolayer tungsten disulfide (WS2) on epitaxial graphene (EG) grown on silicon carbide (SiC). Single-crystalline WS2 flakes with lateral size of hundreds of nanometers are obtained via chemical vapor deposition (CVD) on EG. Microscopic and diffraction analyses indicate that the WS2/EG stack is predominantly aligned with zero azimuthal rotation. The present experiments show that, when perturbed by a scanning probe microscopy (SPM) tip, the WS2 flakes are prone to slide over the graphene surfaces at room temperature. Atomistic force field-based molecular dynamics simulations indicate that, through local physical deformation of the WS2 flake, the scanning tip releases enough energy to the flake to overcome the motion activation barrier and trigger an ultralow-friction rototranslational displacement, that is superlubric. Experimental observations show that, after sliding, the WS2 flakes come to rest with a rotation of nπ/3 with respect to graphene. Moreover, atomically resolved measurements show that the interface is atomically sharp and the WS2 lattice is strain-free. These results help to shed light on nanotribological phenomena in van der Waals (vdW) heterostacks, and suggest that the applicative potential of the WS2/graphene heterostructure can be extended by novel mechanical prospects.

关键词： superlubricity graphene tungsten disulfide scanning tunneling microscopy (STM) two-dimensional (2D) materials nanomechanical

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Male reproductive suppression： not a social affair

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Current Zoology 2017年第5期63卷 573-579页

作者： Z. valentina ZlZZRI Andrea JESSEN Joris M. KOENE Department of Ecological Science--Animal Ecology VU University Amsterdam De Boelelaan 1085 1081 HVAmsterdam The Netherlands

In the animal kingdom there are countless strategies via which males optimize their reproductivesuccess when faced with male-male competition. These male strategies typically fall into two maincategories： pre- and post-copulatory competition. Within these 2 categories, a set of behaviors,referred to as reproductive suppression, is known to cause inhibition of reproductive physiologyand/or reproductive behavior in an otherwise fertile individual. What becomes evident when con-sidering examples of reproductive suppression is that these strategies conventionally encompassreproductive interference strategies that occur between members of a hierarchical social ***, mechanisms aimed at impairing a competitor＇s reproductive output are also present innon-social animals. Yet, current thinking emphasizes the importance of sociality as the primarydriving force of reproductive suppression. Therefore, the question arises as to whether there is anactual difference between reproductive suppression strategies in social animals and equivalentpre-copulatory competition strategies in non-social animals. In this perspective paper we explore abroad taxonomic range of species whose individuals do not repeatedly interact with the same indi-viduals in networks and yet, depress the fitness of rivals. Examples like alteration of male repro-ductive physiology, female mimicry, rival spermatophore destruction, and cementing the rival＇sgenital region in non-social animals, highlight that male pre-copulatory reproductive suppressionand male pre-copulatory competition overlap. Finally, we highlight that a distinction between malereproductive interference in animals with and without a social hierarchy might obscure importantsimilarities and does not help to elucidate why different proximate mechanisms evolved. We there-fore emphasize that male reproductive suppression need not be restricted to social animals.

关键词： indirect sperm transfer, offensive strategies, male pre-copulatory competition, male reproductive suppression, repro-ductive strategies.

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Mucosa-associated lymphoid tissue lymphoma simulating Crohn’s disease:A case report

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World Journal of Clinical Cases 2020年第8期8卷 1454-1462页

作者： Ieva Stundiene Vaidota Maksimaityte valentina Liakina Jonas Valantinas Centre of Hepatology Gastroenterology and DieteticsFaculty of MedicineVilnius UniversityVilnius 03101Lithuania Department of Chemistry and Bioengineering Faculty of Fundamental ScienceVilnius Gediminas Technical UniversityVilnius 10223Lithuania Clinic of Gastroenterology Nephrourology and SurgeryFaculty of MedicineVilnius UniversityVilnius 03101Lithuania

BACKGROUND Differential diagnosis between extranodal marginal zone lymphoma of mucosaassociated lymphoid tissue and inflammatory bowel disease is mainly based on histopathologic evaluation of intestinal biopsies,although there is no single definitive diagnostic investigation and that circumstance can lead to misdiagnosis in particular *** we present a rare,ulcerative form of marginal zone lymphoma which mimics the Crohn’s disease(CD)of upper digestive *** SUMMARY A 50-year-old man was presented with recurrent episodes of malaise and melena also weight *** of the small bowel demonstrated an ulcer in the ***,biopsies showed lymphoplasmacytic *** of CD was *** treatment consisted of prednisone and azathioprine and was followed by azathioprine 100 mg per day with good clinical response in the following 2 years until *** this time the results of endoscopic biopsies derived from proximal wall of stomach revealed Helicobacter pylori-negative marginal zone lymphoma of the gastric *** confirmed atypical CD20-positive cell *** on these biopsies,marginal zone lymphoma of mucosa-associated lymphoid tissue was ***,the contact with the patient was lost until one year later he was hospitalized with nausea,vomiting and severe pain because of gastrointestinal *** months later after laparotomy,the patient was treated with a course of *** remission was observed following 6 cycles of *** This case report highlights the clinical relevance of knowledge and awareness of marginal zone lymphoma simulating CD.

关键词： Crohn disease Mucosa-associated lymphoid tissue lymphoma Rare diagnosis Gastrointestinal diseases Thiopurines Case report

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The interanual rainfall variability in Indonesia corresponding to El Ni?o Southern Oscillation and Indian Ocean Dipole

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Acta Oceanologica Sinica 2019年第7期38卷 57-66页

作者： I Gede Hendrawan Koji Asai Andhita Triwahyuni Diah valentina Lestari Department of Marine Science Udayana UniversityBadungBali 80361Indonesia Graduate School Science and Engineering Yamaguchi UniversityYamaguchi 755-8611Japan Department of Environmental Engineering Faculty of EngineeringMahasaraswati UniversityDenpasarBali 80236Indonesia Center for Remote Sensing and Ocean Sciences Udayana UniversityBadungBali 80361Indonesia

The Impact of the Indian Ocean Dipole(IOD)and the El Nino Southern Oscillation(ENSO)event for Indonesian rainfall has been investigated for the period from 1950 to ***-annual change of IOD and ENSO indices are used to investigate their relationship with Indonesian *** using the wavelet transform method,we found a positive significant correlation between IOD and Indonesian rainfall on the time scale of nearly 2.5–4 ***,the positive significant correlation between ENSO(sea surface temperature anomaly at Nino3.4 area indices)and Indonesian rainfall exists for shorter than 2 years and between 5.5 to 6.5-year time scales.

关键词： IOD ENSO Indonesian rainfall wavelet

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From unstructured texts to semantic story maps

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International Journal of Digital Earth 2023年第1期16卷 234-250页

作者： valentina Bartalesi Gianpaolo Coro Emanuele Lenzi Pasquale Pagano NicolòPratelli Istituto di Scienza e Tecnologie dell’Informazione‘A.Faedo’del Consiglio Nazionale delle Ricerche PisaItaly

Digital maps greatly support storytelling about territories,especially when enriched with data describing cultural,societal,and ecological aspects,conveying emotional messages that describe the territory as a *** maps are interactive online digital narratives that can describe a territory beyond its map by enriching the map with text,pictures,videos,and other multimedia *** paper presents a semi-automatic workflow to produce story maps from textual documents containing territory *** expertfirst assembles one territory-contextual document containing text and ***,automatic processes use natural language processing and Wikidata services to(i)extract key concepts(entities)and geospatial coordinates associated with the territory,(ii)assemble a logically-ordered sequence of enriched story-map events,and(iii)openly publish online story maps and an interoperable Linked Open Data semantic knowledge base for event exploration and inter-story correlation *** workflow uses an Open Science-oriented methodology to publish all processes and *** our workflow,we produced story maps for the value chains and territories of 23 rural European areas of 16 *** numerical evaluation,we demonstrated that territory experts considered the story maps effective in describing their territories,and appropriate for communicating with citizens and stakeholders.

关键词： Story maps semantic web ontology natural language processing narratives e-infrastructures virtual research environments

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Solid electrolyte composite Li4P2O7-Li3PO4 for lithium ion battery

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Progress in Natural Science:Materials International 2020年第2期30卷 168-173页

作者： Evvy Kartini valentina Yapriadi Heri Jodi Maykel Manawan Cipta Panghegar Wahyudianingsih Center for Science and Technology of Advanced Materials National Nuclear Energy Agency 2. Faculty of EngineeringLeeds University 3. Energy EngineeringJakarta State Polytechnic 4. Polytechnique Institute of Nuclear Technology

The researches on solid electrolyte have been significantly increasing due to the safety problem in lithium ion *** lithium phosphates are chosen due to environmentally *** the present study LiPOwas synthesized by solid state reaction using NHHPOand LiCOwith the ratio 1:2 at various temperatures of600 ℃,800 ℃ and 900℃.The products were characterized by x-ray diffraction,scanning electron microscopy and impedance *** x-ray diffraction showed that all samples consisted of two *** was found that the products consisted of 52.44% LiPOand 47.56% LiPO;93.56% LiPOand 6.44% LiPO;and46.27% LiPOand 53.67% LiPOunder the synthesizing temperature of 600 ℃,800℃ and 900 ℃,*** highest ionic conductivity of 3.85 ×10S/m was achieved for composite LiPO-LiPOwith the highest content of 93.56% *** conductivity is higher compared with single phase of LiPO,LiPOand *** increase in ionic conductivity may be due to the mixed anion effects related to the phosphate networks,and it also corresponds to the existence of anorthic phase LiPOwith the space group P-1(2).The crystal lattice analysis showed that the reactant LiPOconsisted of diphosphate groups *** lithium tetrahedral LiOwere linked to POgroups formed a continuous framework containing large voids,available for Li~+ ion transport,and thus it exhibited high conductivity.A composite LiPO-LiPOis a promising solid electrolyte for solid state battery.

关键词： Solid electrolyte LiPO 3 Li 3 PO4 Li 4 P 2 O 7 Ionic conductivity Lithium ion battery

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Computer animations of color markings revealthe function of visual threat signals inNeolamprologus pulcher

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Current Zoology 2017年第1期63卷 45-54页

作者： valentina BALZARINI Michael TABORSKY Fabienne VILLA Joachim G. FROMMEN Institute of Ecology and Evolution Behavioral Ecology Division University of Bern Wohlenstrasse 50a 3032Hinterkappelen Switzerland

Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or motivation. Communication of aggressive propensity is particularly importantin group-living animals with a stable dominance hierarchy, as the outcome of aggressive inter-actions determines the social rank of group members. Neolamprologus pulcher is a cooperativelybreeding cichlid showing frequent within-group aggression. Both sexes exhibit two vertical blackstripes on the operculum that vary naturally in shape and darkness. During frontal threat displaysthese patterns are actively exposed to the opponent, suggesting a signaling function. To investi-gate the role of operculum stripes during contests we manipulated their darkness in computeranimated pictures of the fish. We recorded the responses in behavior and stripe darkness of testsubjects to which these animated pictures were presented. Individuals with initially darker stripeswere more aggressive against the animations and showed more operculum threat *** stripes of test subjects became darker after exposure to an animation exhibiting a paleoperculum than after exposure to a dark operculum animation, highlighting the role of the dark-ness of this color pattern in opponent assessment. We conclude that （i） the black stripes on theoperculum of N. pulcherare a reliable signal of aggression and dominance, （ii） these markings playan important role in opponent assessment, and （iii） 2D computer animations are well suited to elicitbiologically meaningful short-term aggressive responses in this widely used model system ofsocial evolution.

关键词： aggression, agonistic behavior, animated pictures, cichlid fish, communication, cooperation, social, threat display.

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Interferon-gamma and neuropathy: balance between pain and neuroprotection

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Neural Regeneration Research 2022年第12期17卷 2700-2701页

作者： valentina Ferrara Alessandra Toti Carla Ghelardini Lorenzo Di Cesare Mannelli Department of Neuroscience PsychologyDrug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology SectionUniversity of FlorenceFlorenceItaly

Chronic pain is sustained by a phenomenon of hyper-activation of nociceptive neurons both at peripheral(peripheral sensitization)and central(central sensitization)*** onset and maintenance of pain,however,is to be found in the interaction among the various cell populations in the nervous tissue including neurons and glia(Nam et al.,2016).The pathogenesis of neuropathic pain is extremely complex depending on the primary cause of nerve damage,*** nerve injury is associated with a robust inflammatory response while chemotherapy-induced pain is characterized by a modest phlogistic component(Di Cesare Mannelli et al.,2013).Despite these differences,a powerful common concept to explain the cellular mechanisms underlying pain is the activation of glia.

关键词： pain sustained

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