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Androgenic regulation of novel genes in the epididymis

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Asian Journal of Andrology 2007年第4期9卷 545-553页

作者： Bernard Robaire Shayesta Seenundun Mahsa Hamzeh Sophie-Anne lamour Department of Pharmacology and Therapeutics McGill University Montrgal QC H3G1Y6 Canada Department of Obstetrics and Gynecology McGill University Montrgal QC H3G1Y6 Canada

The epididymis is critically dependent on the presence of the testis. Although several hormones, such as retinoids and progestins, and factors secreted directly into the epididymal lumen, such as androgen binding protein and fibroblast growth factor, might play regulatory roles in epididymal function, testosterone （T） and its metabolites, dihydrotestosterone （DHT） and estradiol （E2）, are accepted as the primary regulators of epididymal structure and functions, with the former playing the greater role. To ascertain the molecular action of androgens on the epididymis, three complementary approaches were pursued to monitor changes in gene expression in response to different hormonal milieux. The first was to establish changes in gene expression along the epididymis as androgenic support is withdrawn. The second was to determine the sequence of responses that occur in an androgen deprived tissue upon re-administration of the two metabolites of T, DHT and E2. The third was to study the effects of androgen withdrawal and re-administration on gene expression in immortalized murine caput epididymidal principal cells. Specific responses were observed under each of these conditions, with an expected major difference in the panoply of genes expressed upon hormone withdrawal and re-administration; however, some key common features were the common roles of genes in insulin like growth factor/epidermal growth factor and the relatively minor and specific effects of E2 as compared to DHT. Together, these results provide novel insights into the mechanisms of androgen regulation in epididymal principal cells. （Asian J Androl 2007 July; 9： 545-553）

关键词： epididymis cell culture androgen withdrawal dihydrotestosterone estradiol apoptosis survivin insulin like growth factor

Molecular Cloning, Expression and Characterization of Ribokinase of Leishmania major

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Acta Biochimica et Biophysica Sinica 2007年第6期39卷 462-466页

作者： Patrick. O. J. OGBUNUDE Nadia lamour Michael P. BARRETT Department of Medical Biochemistry University of Nigeria Enugu 1000004 Nigeria Department of Biochemistry and Massey Cancer Center Virginia Commonwealth University Richmond 23298-0614 USA Institute of Biomedical and Life Sciences Division of Infection and Immunity Joseph Black Building University of Glasgow Glasgow G12 8QQ UK

Ribokinase （EC 2.***.7.15） from Leishmania major was cloned, sequenced and overexpressed in Escherichia coli. The gene expressed an active enzyme that had comparable activity to the same enzyme studied in E. coli. It specifically phosphorylated D-ribose. Under defined conditions, the Km for the substrates D-ribose and ATP were 0.3±0.04 mM and 0.2±0.02mM, respectively. The turnover numbers of the enzyme for the substrates were 10.8s-1and 10.2 s-1, respectively. The enzyme product ribose 5-phosphate inhibited the phosphorylation of D-ribose with an apparent Kiof 0.4 mM, which is close to the Km（0.3mM） of Dribose, suggesting that it might play a role in regulating flux through the enzyme.

关键词： Leishmania major D-ribose ribokinase molecular cloning

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净水进万家

疯狂英语（新读写） 2022年第4期 39-39页

作者： Luke lamour 不详

全世界仍有许多人无法获得洁净的水,一个非营利组织正在努力改变这种情况。

关键词：非营利组织净水洁净

Genetic Diversity of the Pepper Pathogen <i>Phytophthora capsici</i>on Farms in the Amazonian High Jungle of Peru

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American Journal of Plant Sciences 2011年第3期2卷 461-466页

作者： Jon Hulvey Oscar Hurtado-Gonzalez Liliana Aragón-Caballero Daniel Gobena Dylan Storey Ledare Finley Kurt lamour 不详

Phytophthora capsici is an important oomycete pathogen of Capsicum peppers worldwide. Populations of P. capsici recovered from coastal regions in Peru were previously shown to be dominated by a single clonal lineage referred to as PcPE-1. During 2008, 219 isolates of P. capsici were collected from Capsicum pubescens (Rocoto), C. annum (Pimento), and C. baccatum (Aji) at 9 farms in the Amazonian high jungle in the areas surrounding Oxapampa, and one coastal location, Carabayllo. Two isolates of P. capsici were also recovered from Cyclanthera pedata (Caigua fruit) near one field. All isolates were characterized using a panel of eight single nucleotide polymorphism (SNP) markers that are fixed for heterozygosity in the PcPE-1 lineage. A subset of isolates was also characterized using amplified fragment length polymorphism (AFLP) markers. Nine discreet SNP multi-locus genotypes were identified, and the PcPE-1 lineage was recovered from all of the field sites. Both A1 and A2 mating types were recovered from two sites. The implications of the genotypic diversity and distribution identified in this study are discussed.

关键词： Population Genetics DNA Markers Clonality Asexual Reproduction Sexual Reproduction

神经酰胺激酶对神经酰胺转移蛋白提供的神经酰胺的利用：在类二十烷酸合成细胞器中的定位

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生物技术世界 2007年第4期4卷 76-76页

作者： Nadia F.lamour 魏荣瑄（译）

在哺乳动物细胞中，神经酰胺激酶（CERK）可以使神经酰胺磷酸化，生成神经酰胺-1-磷酸（CIP）。在本研究中，作者确定了C1P在细胞中的主要类型是C16：0C1P和C18：0C1P．说明神经酰胺激酶通过神经酰胺转移蛋白（CERT）转移至反面高尔基... 详细信息

在哺乳动物细胞中，神经酰胺激酶（CERK）可以使神经酰胺磷酸化，生成神经酰胺-1-磷酸（CIP）。在本研究中，作者确定了C1P在细胞中的主要类型是C16：0C1P和C18：0C1P．说明神经酰胺激酶通过神经酰胺转移蛋白（CERT）转移至反面高尔基体。为了证明这一说法，作者用RNA干扰对神经酰胺转移蛋白进行了下调表达处理，结果表明。

关键词：神经酰胺细胞器蛋白激酶定位合成烷酸哺乳动物细胞