检索结果-南通市图书馆

Genes associated with testicular germ cell tumors and testicular dysgenesis in patients with testicular microlithiasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2018年第6期20卷 593-599页

作者： Ilya S Dantsev Evgeniy v Ivkin Aleksey A Tryakin Dmitriy N Godlevski Oleg Yu Latyshev victoriya v Rudenko Dmitry S Mikhaylenko v.acheslav.b chernykh Elena A volodko Aleksey b Okulov Oleg b Loran Marina v Nemtsova Russian Medical Academy ef Postgraduate Education Moscow 123995 Russia N. N. blokhin Russian Cancer Research Center Moscow 115478 Russia Research Centre of Medical Genetics Moscow 115478 Russia I. M. Sechenov First Moscow State Medical University Moscow 119991 Russia N. I. Pirogov Russian National Research Medical University Moscow 117997 Russia.

Testicular microlithiasis （TM） is one of the symptoms of testicular dysgenesis syndrome （TDS）. TM is particularly interesting as an informative marker of testicular germ cell tumors （TGCTs）. KIT ligand gene （KITLG）, bCL2 antagonist/killer 1 （bAK1）, and sprouty RTK signaling antagonist 4 （SPRY4） genes are associated with a high risk of TGCTs, whereas bone morphogenetic protein 7 gene （bMP7）, transforming growth factor beta receptor 3 gene （TGFbR3）, and homeobox D cluster genes （HOXD） are related to TDS. Using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis, we investigated allele and genotype frequencies for KITLG （rs995030, rs1508595）, SPRY4 （rs4624820, rs6897876）, bAK1 （rs210138）, bMP7 （rs388286）, TGFbR3 （rs12082710）, and HOXD （rs17198432） in 142 TGCT patients, 137 TM patients, and 153 fertile men （control group）. We found significant differences in the KITLG GG_rs995030 genotype in TM （P= 0.01） and TGCT patients （P = 0.0005） compared with the control. We also revealed strong associations between KITLGrs1508595 and TM （G allele, P = 0.003; GG genotype, P= 0.01） and between KITLG_rs1508595 and TGCTs （G allele, P-- 0.0001; GG genotype, P = 0.0007）. Moreover, there was a significant difference in bMP7_rs388286 between the TGCT group and the control （T allele, P = 0.00004; TT genotype, P = 0.00006） and between the TM group and the control （T allele, P= 0.04）. HOXDalso demonstrated a strong association with TGCTs （rs17198432 A allele, P = 0.0001; AA genotype, P = 0.001）. Furthermore, significant differences were found between the TGCT group and the control in the bAK1_rs210138 G allele （P= 0.03） and the GG genotype （P= 0.01）. KITLG and bMP7genes, associated with the development of TGCTs, may also be related to TM. In summary, the KITLG GG_rs995030, GG_rs1508595, bMP7 TT_rs388286, HOXD AA_rs17198432, and bAK1 GG_rs210138 genotypes were associated with a high risk of TGCT development.

关键词： associations genotype high risk testicular dysgenesis syndrome testicular microlithiasis

Global methylation status of sperm DNA in carriers of chromosome structural aberrations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第1期19卷 117-124页

作者： Marta Olszewska Miroslawa Z barciszewska Monika Fraczek Nataliya Huleyuk v.acheslav.b chernykh Danuta Zastavna Jan barciszewski Maciej Kurpisz Department of Reproductive biology and Stem Cells Institute of Human Genetics Polish Academy of Sciences Strzeszynska 32 60-479 Poznan Poland Department of RNA biology Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14 61-704 Poznan Poland Institute of Hereditary Pathology Ukrainian Academy of Medical Sciences Lysenko Street 31a 79000 Lviv Ukraine Research Centre for Medical Genetics Russian Academy of Medical Sciences Moskvorechie Street 1115478 Moscow Russian Federation.

Male infertility might be clearly associated with aberrant DNA methylation patterns in human spermatozoa. An association between oxidative stress and the global methylation status of the sperm genome has also been suggested. The aim of the present study was to determine whether the global sperm DNA methylation status was affected in the spermatozoa of carriers of chromosome structural aberrations. The relationships between the 5-methylcytosine （msC） levels in spermatozoa and chromatin integrity status were evaluated. The study patients comprised male carriers of chromosome structural aberrations with reproductive failure （n = 24）, and the controls comprised normozoospermic sperm volunteers （n = 23）. The global msC level was measured using thin-layer chromatography （TLC） and immunofluorescence （IF） techniques. The sperm chromatin integrity was assessed using aniline blue （Ab） staining and TUNEL assay. The mean msC levels were similar between the investigated chromosome structural aberrations carriers （P） and controls （K）. However, sperm chromatin integrity tests revealed significantly higher values in chromosomal rearrangement carriers than in controls （P 〈 0.05）. Although the potential relationship between sperm chromatin integrity status and sperm DNA fragmentation and the msC level juxtaposed in both analyzed groups （P vs K） was represented in a clearly opposite manner, the low chromatin integrity might be associated with the high hypomethylation status of the sperm DNA observed in carriers of chromosome structural aberrations.

关键词： 5-methylcytosine chromatin integrity chromosomal rearrangement chromosome translocation global DNA methylation male infertility

Design of broadband terahertz vector and vortex beams: II. Holographic assessment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Advanced Manufacturing) 2022年第4期3卷 14-26页

作者： Nikolay v.Petrov bogdan Sokolenko Maksim S.Kulya Andrei Gorodetsky Aleksey v.chernykh Digital and Display Holography Laboratory ITMO UniversitySt.Petersburg 197101Russia v.I.vernadsky Crimean Federal University Simferopol 295007Russia School of Physics and Astronomy University of BirminghamBirmingham B152TTUK

In this paper,we demonstrate the capabilities of the terahertz pulse time-domain holography in visualisation,simulation,and assessment of broadband THz vortex beam formation dynamics upon its shaping by elements of beam converter,and further propagation and *** adding Jones matrix formalism to describe broadband optical elements,we highlight the differences in the spatio-spectral and spatio-temporal structure of the formed vortex and vector beams dependence on the modulator used and visualise their modal *** influence of diffraction field structure from each element in the broadband vortex modulator is revealed in numerical simulation and the formed beams are analysed against the simplified Laguerre-Gaussian beam model.

关键词： Terahertz radiation vortex beams vector beams Achromatic wave plates broadband terahertz radiation Digital holography Terahertz holography

Design of broadband terahertz vector and vortex beams: I. Review of materials and components

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Advanced Manufacturing) 2022年第4期3卷 126-144页

In this paper,we review the existing approaches for vortex and vector beam shaping and generation in the terahertz frequency *** particular focus of this review is on the possibility of homogeneous topological charge formation in the ultra-wide spectral interval inherent to ultrashort terahertz *** review the available materials and components,analyse proposed and potentially possible solutions for broadband terahertz vortex and vector beam shaping,compare all developed approaches,and put forward a unified concept for constructing passive shapers of such beams from the existing component base.

关键词： Terahertz radiation vortex beams vector beams Achromatic wave plates broadband terahertz radiation Digital holography Terahertz holography Total internal reflection Metasurface THz liquid crystals Geometric phase

Modelling One-Pot Method for Synthesis of 2,3-Dihydro-1<i>H</i>-pyrrolo[2,1-<i>c</i>][1,4]benzothiazine 5,5-Dioxides and Their Homologues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Organic Chemistry 2013年第2期3卷 125-135页

作者： Oleksandr Yu Grevtsov Oleg v. Zaremba Anna b. bondarenko Oleksandr G. Drushlyak Sergiy M. Kovalenko valentyn P. chernykh National University of Pharmacy Kharkiv Ukraine

A facile method for synthesis of 2,3-dihydro-1H-pyrrolo[2,1-c][1,4]benzothiazines by interaction of methylenactive (2-fluorophenyl)sulfones with homologues of either 5-methoxy-3,4-dihydro-2H-pyrrole or 5-(methylthio)-... 详细信息

关键词： Sulfones Lactims Cyclization Heterocycles Nucleophilic Aromatic Substitution

Antitumor efficacy of multi-target in situ vaccinations with CpG oligodeoxynucleotides,anti-OX40,anti-PD1 antibodies,and aptamers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of biomedical Research 2023年第3期37卷 194-212页

作者： Anastasia S.Proskurina vera S.Ruzanova Genrikh S.Ritter Yaroslav R.Efremov Zakhar S.Mustafin Sergey A.Lashin Ekaterina A.burakova Alesya A.Fokina Timofei S.Zatsepin Dmitry A.Stetsenko Olga Y.Leplina Alexandr A.Ostanin Elena R.chernykh Sergey S.bogachev Institute of Cytology and Genetics Siberian Branch of the Russian Academy of SciencesNovosibirsk 630090Russia Department of Natural Sciences Novosibirsk State UniversityNovosibirsk 630090Russia Department of Physics Novosibirsk State UniversityNovosibirsk 630090Russia Department of Chemistry Moscow State UniversityMoscow 119991Russia Research Institute of Fundamental and Clinical Immunology Novosibirsk630099Russia

To overcome immune tolerance to cancer,the immune system needs to be exposed to a multi-target action ***,we investigated the activating effect of CpG oligodeoxynucleotides(ODNs),mesyl phosphoramidate CpG ODNs,anti-OX40 antibodies,and OX40 RNA aptamers on major populations of immunocompetent cells ex *** analysis of the antitumor effects of in situ vaccination with CpG ODNs and anti-OX40 antibodies,as well as several other combinations,such as mesyl phosphoramidate CpG ODNs and OX40 RNA aptamers,was *** against programmed death 1(PD1)checkpoint inhibitors or their corresponding PD1 DNA aptamers were also added to vaccination regimens for analytical *** scenarios were considered:a weakly immunogenic Krebs-2 carcinoma grafted in CbA mice;a moderately immunogenic Lewis carcinoma grafted in C57black/6 mice;and an immunogenic A20 b cell lymphoma or an Ehrlich carcinoma grafted in bALb/c *** anti-PD1 antibodies(CpG+αOX40+αPD1)to in situ vaccinations boosts the antitumor *** to be used instead of antibodies,aptamers also possess antitumor activity,although this effect was less *** strongest effect across all the tumors was observed in highly immunogenic A20 b cell lymphoma and Ehrlich carcinoma.

关键词： immunogenicity Krebs-2 carcinoma Lewis carcinoma Ehrlich carcinoma A20 b cell lymphoma mesyl phosphoramidate