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Nanomotion of bacteria to determine metabolic profile

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Nanotechnology and Precision Engineering 2024年第1期7卷 1-9页

作者： s.N.Pleskova E.v.Lazarenko N.A.Bezrukov R.N.Kriukov A.v.Boryakov M.E.Dokukin s.I.surodin Research Laboratory of scanning Probe Microscopy Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Department of Nanotechnology and Biotechnology Nizhny Novgorod State Technical University n.a.R.E.AlekseevNizhny Novgorod 603950Russia sarov Physics and Technology Institute National Research Nuclear University MEPhISarov 607186Russia

In addition to their visible motion such as swimming(e.g.,with the help ofﬂagella),bacteria can also exhibit nanomotion that is detectable only with highly sensitive instruments,and this study shows that it is possible to detect bacterial nanomotion using an AFM detection *** results show that the nanomotion characteristics depend on the bacterial strain,and that nanomotion can be used to sense the metabolic activity of bacteria because the oscillations are sensitive to the food preferences of the bacteria and the type of surrounding medium.

关键词： Nanomotion Bacteria Cantilever Oscillation Atomic force microscopy Metabolism

Elucidating elusive quaternary selenide EuCeCuse3:synthesis,crystal structure,properties and theoretical studies

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Journal of Rare Earths 2024年第1期42卷 163-171,I0006页

作者： Maxim v.Grigoriev Anna v.Ruseikina Maxim s.Molokeev vladimir A.Chernyshev Aleksandr s.Aleksandrovsky Alexander s.Krylov svetlana N.Krylova Nikolai P.shestakov Dmitriy A.velikanov Alexander A.Garmonov Alexey v.Matigorov Evgeny A.Ostapchuk Thomas schleid Damir A.safin Laboratory of Theory and Optimization of Chemical and Technological Processes University of TyumenVolodarskogo Str.6625003TyumenRussian Federation Kirensky Institute of Physics Federal Research Center KSC SB RASAkademgorodok 50 Bld.38660036KrasnoyarskRussian Federation Department of Physics Far Eastern State Transport UniversitySerysheva Str.47680021KrasnoyarskRussian Federation Institute of Natural sciences and Mathematics Ural Federal University Named After the First President of Russia B.N.YeltsinMira Str.19620002EkaterinburgRussian Federation Department of Photonics and Laser Technology Siberian Federal UniversitySvobodnii Ave.79660079KrasnoyarskRussian Federation Institute of Physics and Technology University of TyumenVolodarskogo Str.6625003 TyumenRussian Federation Institute for Inorganic Chemistry University of StuttgartPfaffenwaldring 55D-70569StuttgartGermany scientific and Educational and Innovation Center for Chemical and Pharmaceutical Technologies Ural Federal University named after the First President of Russia B.N.YeltsinMira Str.19620002 EkaterinburgRussian Federation University of Tyumen Volodarskogo Str.6625003TyumenRussian Federation

We report on the novel heterometallic quaternary selenide EuCeCuse3,the fabrication of which has been a challenge until this *** structure of the reported selenide was elucidated from the powder X-ray diffraction data,which revealed the formation of EuCeCuse3with excellent yield(96.7%)accompanied with a minor fraction of Cese2(3.3%),and was best solved in orthorhombic space group Pnma with the BaLaCus3structural ***,the crystal structure of the title compound completes the row of the heterometallic quaternary selenides EuRECuse3(RE=La,Pr,Nd,sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Yb,Lu,Y),of which the cerium-based derivative exclusively belongs to the BaLaCus3structural *** distortion of the Cuse4polyhedron was compared for the whole series of EuRECuse3compounds using theτ4-descriptor for four coordinated ions,which revealed the highest degree of distortion for the Ce3+-containing selenide,followed by the La3+-based ***,the crystallographic and geometrical parameters of the reported selenide were discussed in comparison to the Ce3+-based sulfides srCeCus3and *** initio calculations of the crystal structure,a phonon spectrum and elastic constants for the crystal of EuCeCuse3were also *** types and wavenumbers of fundame ntal modes were determined and the involvement of ions participating in the phonon modes was *** experimental IR spectrum of the reported selenide was interpreted and found to be in agreement with the calculated *** experimental direct band gap of EuCeCuse3was measured to be 1.36 ev that is consistent with the concept of its origin due to interband transitions between orbitals emerging mainly from 4f(valence band)and 5d(conduction band)levels of the Eu2+*** dependence of the Young’s modulus on the direction demonstrates the anisotropy of the elastic properties,while the vickers hardness for EuCeCuse3was calculated to be 5.2 ***,the title compound is paramagnetic above 4 K.

关键词： Quaternary selenide synthesis Crystal structure Ab initio calculations Magnetic measurements spectroscopy

Myelin histology:a key tool in nervous system research

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Neural Regeneration Research 2024年第2期19卷 277-281页

作者： Óscar Darío García-García víctor Carriel Jesús Chato-Astrain Department of Histology Tissue Engineering GroupUniversity of Granada&Instituto de Investigación Biosanitaria ibs.GRANADAGranadaSpain

The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve *** to the complex structure and composition of myelin,various histological techniques have been developed over the centuries to evaluate myelin under normal,pathological or experimental ***,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience *** this review,we provide an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining *** the lipoprotein nature of myelin,the main features and technical details of the different available methods that can be used to evaluate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.

关键词： fluorescence microscopy histology light microscopy lipid histochemistry metallographic techniques myelin histochemistry myelin immunohistochemistry myelin structure&composition myelin ultrastructural evaluation tissue fixation&processing

The effect of long-term application of nitrogen-rich fertilizers on soil resistome:A study of conventional and organic cropping systems

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soil Ecology Letters 2024年第3期6卷 135-150页

作者： Alexey s.vasilchenko Evgenii O.Burlakov Darya v.Poshvina Denis s.Gruzdev sergey v.Kravchenko Aleksandr v.Iashnikov Ning Ling Anastasia v.vasilchenko Laboratory of Antimicrobial Resistance Institute of Environmental and Agricultural Biology(X-BIO)Tyumen State UniversityTyumenRussia Research Institute of Mathematics Physics and Computer ScienceDerzhavin Tambov State UniversityTambovRussia International Integrated Research Laboratory for Climate Change Land Use and BiodiversityInstitute of Environmental and Agricultural Biology(X-BIO)Tyumen State UniversityTyumenRussia sciBear OU Tartu Mnt 67/1-13bKesklinna Linnaosa10115 TallinnEstonia Center for Grassland Microbiome State Key Laboratory of Grassland Agro-EcosystemsCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000China

Metagenomic studies of various soil environments have previously revealed the widespread distribution of antibiotic resistance genes(ARGs)around the *** this study,we applied shotgun metagenomics to investigate differences in microbial communities and resistomes in Chernozem soils that have been under long-term organic and conventional cropping *** organic cropping system was seeded with Triticum spelta without any *** conventional cropping system was seeded with Tríticum durum Desf and used mineral fertilizer(NPK),that resulted in an increased amount of total and available carbon and nitrogen in *** all samples,we identified a total of 21 ARG classes,among which the dominant were vancomycin,tetracycline and *** of soil microbial communities revealed differences between the studied fields in the relative abundances of 14 and 53 genera in topsoil and subsoil,*** analysis showed significant correlations(positive and negative)among 18 genera and 6 ARGs,as well as between these ARGs and some chemical properties of *** analysis of metagenome-assembled genomes revealed that Nitrospirota,Thermoproteota,Actinobacteriota and Binatota phyla of archaea and bacteria serve as hosts for glycopeptide and fluoroquinolone/tetracycline ***,the data obtained enrich knowledge about the consequences of human agricultural activities in terms of soil microbiome modification and highlight the role of nitrogen cycling taxa,including uncultivated genera,in the formation of soil resistome.

关键词： soil microbiome inorganic fertilizer nitrogen cycle uncultured bacteria chemolithotrophs Binatia

Deep Learning-Based Classification of Rotten Fruits and Identification of shelf Life

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Computers, Materials & Continua 2024年第1期78卷 781-794页

作者： s.sofana Reka Ankita Bagelikar Prakash venugopal v.Ravi Harimurugan Devarajan Centre for smart Grid Technologies School of Electronics EngineeringVellore Institute of TechnologyChennai600127India school of Electronics Engineering Vellore Institute of TechnologyChennai600127India Department of Electrical Engineering Dr.B R Ambedkar National Institute of TechnologyJalandharPunjab144008India

The freshness of fruits is considered to be one of the essential characteristics for consumers in determining their quality,flavor and nutritional *** primary need for identifying rotten fruits is to ensure that only fresh and high-quality fruits are sold to *** impact of rotten fruits can foster harmful bacteria,molds and other microorganisms that can cause food poisoning and other illnesses to the *** overall purpose of the study is to classify rotten fruits,which can affect the taste,texture,and appearance of other fresh fruits,thereby reducing their shelf *** agriculture and food industries are increasingly adopting computer vision technology to detect rotten fruits and forecast their shelf ***,this research work mainly focuses on the Convolutional Neural Network’s(CNN)deep learning model,which helps in the classification of rotten *** proposed methodology involves real-time analysis of a dataset of various types of fruits,including apples,bananas,oranges,papayas and ***,machine learningmodels such as GaussianNaïve Bayes(GNB)and random forest are used to predict the fruit’s shelf *** results obtained from the various pre-trained models for rotten fruit detection are analysed based on an accuracy score to determine the best *** comparison to other pre-trained models,the visual geometry group16(vGG16)obtained a higher accuracy score of 95%.Likewise,the random forest model delivers a better accuracy score of 88% when compared with GNB in forecasting the fruit’s shelf *** developing an accurate classification model,only fresh and safe fruits reach consumers,reducing the risks associated with contaminated ***,the proposed approach will have a significant impact on the food industry for efficient fruit distribution and also benefit customers to purchase fresh fruits.

关键词： Rotten fruit detection shelf life deep learning convolutional neural network machine learning gaussian naïve bayes random forest visual geometry group16

Effect of proton irradiation on the optical properties of thermal control coating based on polystyrene with silica filler

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Propulsion and Power Research 2024年第1期13卷 1-11页

作者： N.I.Cherkashina v.I.Pavlenko M.M.Mikhailov A.N.Lapin s.A.Yuriev R.v.sidelnikov D.s.Romanyuk Belgorod state Technological University named after v.G.shoukhov Kostyukov Str.46Belgorod 308012Russia Tomsk state University of Control systems and Radioelectronics 40Prospect LeninaTomsk 634050Russia

This paper presents the results of studying the surface properties changes of a ther-moregulating coating based on polystyrene and silicaﬁller after proton irradiation with an en-ergy of 50 kev at afluence of 3�1015 cm�*** proton irradiation,the values of the contact angle of wetting with water increase by 3.5%and 14.9%for polystyrene and the coating,*** free surface energy(energy of the surface layer)of polystyrene and the coat-ings before and after proton irradiation was calculated using the Owens-Wendt-Rabel-Kaelble *** was a signiﬁcant increase in the polarity of the polystyrene surface(gp increased by a factor of 2.2)after proton *** the coating,an increase in gp by a factor of 3.89 was observed after proton *** changes in the coating were presented by IR Fourier spectroscopy.A slight decrease in the absorption intensity of all characteristic bands compared to the unirradiated sample was *** was found that the irradiation of the coating with protons led to the formation of macromolecules with hydroxyl,carbonyl,and carboxyl bonds,as well as the formation of dimeric and oligomeric siloxane *** was also found that after irradiation of a pure polystyrene sample with protons,the value of the solar absorption as increased by only 4.2%;whereas for the coating with silicaﬁller,the value of as increased by 28.6%.

关键词： Thermal control coating Proton irradiation Hydrophobicity Free surface energy Radiation crosslinking

Ranking environmental and edaphic attributes driving soil microbial community structure and activity with special attention to spatial and temporal scales

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mLife 2024年第1期3卷 21-41页

作者： vadakattu v.s.R.Gupta James M.Tiedje CsIRO Agriculture&Food UrrbraeSouth AustraliaAustralia Centre for Microbial Ecology Michigan State UniversityEast LansingMichiganUSA

The incredibly complex soil microbial communities at small scales make their analysis and identification of reasons for the observed structures *** community structure is mainly a result of the inoculum(dispersal),the selective advantages of those organisms under the habitat-based environmental attributes,and the ability of those colonizers to sustain themselves over *** soil is protective,and its microbial inhabitants have long adapted to varied soil conditions,significant portions of the soil microbial community structure are likely ***,a substantial portion of the community will not correlate to often measured soil *** suggest that the drivers be ranked on the basis of their importance to the fundamental needs of the microbes:(i)those that supply energy,i.e.,organic carbon and electron acceptors;(i)environmental effectors or stressors,i.e.,pH,salt,drought,and toxic chemicals;(ii)macro-organism associations,i.e.,plants and their seasonality,animals and their fecal matter,and soil fauna;and(iv)nutrients,in order,N,P,and probably of lesser importance,other micronutrients,and *** relevance of drivers also varies with spatial and time scales,for example,aggregate to field to regional,and persistent to dynamic populations to transcripts,and with the extent of phylogenetic difference,hence phenotypic differences in organismal *** present a summary matrix to provide guidance on which drivers are important for particular studies,with special emphasis on a wide range of spatial and temporal scales,and illustrate this with genomic and population(rRNA gene)data from selected studies.

关键词： abundance community structure diversity functional microbiome microorganisms

The influence of resistance exercise training prescription variables on skeletal muscle mass,strength,and physical function in healthy adults:An umbrella review

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Journal of sport and Health science 2024年第1期13卷 47-60页

作者： Jonathan C.Mcleod Brad s.Currier Caroline v.Lowisz stuart M.Phillips Department of Kinesiology Faculty of ScienceMcMaster UniversityHamilton L8S4L8Canada

Purpose:The aim of this umbrella review was to determine the impact of resistance training(RT)and individual RT prescription variables on muscle mass,strength,and physical function in healthy ***:Following Preferred Reporting Items for systematic Reviews and Meta-Analyses(PRIsMA)guidelines,we systematically searched and screened eligible systematic reviews reporting the effects of differing RT prescription variables on muscle mass(or its proxies),strength,and/or physical function in healthy adults aged>18 ***:We identified 44 systematic reviews that met our inclusion *** methodological quality of these reviews was assessed using A Measurement Tool to Assess systematic Reviews;standardized effectiveness statements were *** found that RT was consistently a potent stimulus for increasing skeletal muscle mass(4/4 reviews provide some or sufficient evidence),strength(4/6 reviews provided some or sufficient evidence),and physical function(1/1 review provided some evidence).RT load(6/8 reviews provided some or sufficient evidence),weekly frequency(2/4 reviews provided some or sufficient evidence),volume(3/7 reviews provided some or sufficient evidence),and exercise order(1/1 review provided some evidence)impacted RT-induced increases in muscular *** discovered that 2/3 reviews provided some or sufficient evidence that RT volume and contraction velocity influenced skeletal muscle mass,while 4/7 reviews provided insufficient evidence in favor of RT load impacting skeletal muscle *** was insufficient evidence to conclude that time of day,periodization,inter-set rest,set configuration,set end point,contraction velocity/time under tension,or exercise order(only pertaining to hypertrophy)influenced skeletal muscle adaptations.A paucity of data limited insights into the impact of RT prescription variables on physical ***:Overall,RT increased muscle mass,strength,and physical function compared to no ***

关键词： Hypertrophy Resistance training Resistance training prescription variables strength Umbrella review

Enhancing production monitoring: A back allocation methodology to estimate well flow rates and assist well test scheduling

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PETROLEUM REsEARCH 2024年第3期9卷 369-379页

作者： Chaves, G. s. Ferreira Filho, v. J. M. Univ Fed Rio De Janeiro Rio De Janeiro Brazil

Production flow rates are crucial to make operational decisions, monitor, manage, and optimize oil and gas fields. Flow rates also have a financial importance to correctly allocate production to fiscal purposes required by regulatory agencies or to allocate production in fields owned by multiple operators. Despite its significance, usually only the total field production is measured in real time, which requires an alternative way to estimate wells' production. To address these challenges, this work presents a back allocation methodology that leverages real-time instrumentation, simulations, algorithms, and mathematical programming modeling to enhance well monitoring and assist in well test scheduling. The methodology comprises four modules: simulation, classification, error calculation, and optimization. These modules work together to characterize the flowline, wellbore, and reservoir, verify simulation outputs, minimize errors, and calculate flow rates while honoring the total platform flow rate. The well status generated through the classification module provides valuable information about the current condition of each well (i.e. if the well is deviating from the latest well test parameters), aiding in decision-making for well testing scheduling and prioritizing. The effectiveness of the methodology is demonstrated through its application to a representative offshore oil field with 14 producing wells and two years of daily production data. The results highlight the robustness of the methodology in properly classifying the wells and obtaining flow rates that honor the total platform flow rate. Furthermore, the methodology supports well test scheduling and provides reliable indicators for well conditions. By utilizing real-time data and advanced modeling techniques, this methodology enhances production monitoring and facilitates informed operational decision-making in the oil and gas industry. (c) 2024 The Authors. Publishing services provided by Elsevier B.v. on be

关键词： Production estimation Real-time production Production monitoring Back allocation Well test scheduling Flow rate estimation 2-PHAsE FLOW