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Gamma Irradiation of Embryogenic Callus Cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)

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Agricultural Sciences in China 2008年第9期7卷 1147-1152页

作者： vikas Y Patade P Suprasanna v a bapat Plant Cell Culture Technology Section Nuclear Agriculture & Biotechnology DivisionBhabha Atomic Research Centre

Radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular Indian sugarcane （Saccharum officinarum L.） cv. CoC-671. Embryogenic calli were gamma irradiated and exposed to different levels of NaCl （42.8, 85.6, 128.3, 171.1,213.9, 256.7, 299.5, or 342.2 mM）. The relative growth rate （RGR） decreased progressively with increasing salt stress and was the least with a salt stress of 256.7 mM （0.25±0.009）, almost 10 fold lesser than the control. The RGR was significantly lower in 85.6 mM and higher salt stressed calli than the control. The survival percent also decreased, with an increase in NaCl concentration. In case of 10 and 20 Gy irradiated calli, regeneration was observed up to 85.6 mM NaCl selection, medium, whereas, higher treatments （128.3 mM and beyond） exhibited browning initially. However, in the subsequent subcultures, regeneration was obtained in the case of 10 and 20 Gy irradiated calli on 128.3 and 171.1 mM NaCl selections. Higher dose of gamma irradiation （40 Gy） also showed regeneration, but only with 85.6 mM NaCI selection. The unirradiated calli regenerated the highest number of plantlets followed by 10 and 20 Gy irradiated calli on salt selection. A total of 147 plantlets were selected from different salt levels. The salt selected plants are being tested for their field performance.

关键词： sugarcane in vitro mutagenesis in vitro selection salt tolerance

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Potato： A Favorable Crop for Plant Molecular Farming

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中国马铃薯 2006年第5期20卷 290-297页

作者： Sunil Kumar G. B Ganapathi T R bapat v A Plant Cell Culture Technology Section Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre Trombay Mumbai 400085 India

Potato is one of the important food crops with a high yield potential and nutritional value. It has been used extensively for molecular farming to produce vaccines, antibodies and industrial enzymes. It has several desirable attributes as a favorable crop for the production of recombinant proteins. Potato tubers were employed for bulk production of recombinant antibodies. vaccine production in potato has progressed to human clinical trials. Human milk proteins were successfully expressed in potato tubers. Potato hairy roots offer as another attractive system for the production of useful recombinant proteins both as intra cellular and secreted forms. This review describes the use of potato as a prospective host for plant molecular farming.

关键词： potato vaccines antibodies hepatitis B microtubers hairy roots

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维普期刊数据库

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人心果果肉及胚乳的组织培养

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世界热带农业信息 1986年第1期 66-69页

作者： v.A.bapat 傅萍

本文报道用人心果果肉和胚乳做培养材科对,它们对营养的需要及其生长情况。材料和方法从孟买的特朗贝田间实验站种植园中,采收接近成熟的人心果果实和种子。果实用

关键词：胚乳人心果观果树木创伤组织愈伤组织培养(生物) 组织培养外植体培养果实形态果肉

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