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脾酪氨酸激酶抑制剂对脂多糖诱导巨噬细胞炎症反应的影响及机制

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细胞与分子免疫学杂志 2013年第10期29卷 1024-1027页

作者：崔树娜 ursula Bilitewski 扬州大学医学院江苏扬州225001 Helmholtz Center for Infection Research Braunschweig Germany 38124

目的研究脾酪氨酸激酶（Syk）在LtX3诱导的Toll样受体4（TLR4）信号转导通路的作用。方法用100ng／mL脂多糖和不同浓度Syk抑制剂分别处理RAW264．7巨噬细胞后，采用Alarmar blue方法检测细胞增殖；采用Griess试剂检测细胞培养液中NO的... 详细信息

目的研究脾酪氨酸激酶（Syk）在LtX3诱导的Toll样受体4（TLR4）信号转导通路的作用。方法用100ng／mL脂多糖和不同浓度Syk抑制剂分别处理RAW264．7巨噬细胞后，采用Alarmar blue方法检测细胞增殖；采用Griess试剂检测细胞培养液中NO的含量；Westernblot法检测诱导型一氧化氮合成酶（iNOS）的蛋白表达；ELISA检测巨噬细胞中TNF-α和IL-6含量，RAW—Blue巨噬细胞检测核因子KB（NF—KB）和激活子蛋白-1（AP-1）的活化。结果Syk抑制剂作用于RAW264．7巨噬细胞24h，（0．5～14．0）μmol／L的Syk抑制剂对细胞增殖无明显影响，但当剂量超过1．5μmol／L时，能显著抑制LPS刺激的RAW264．7巨噬细胞NO的产生和iNOS的蛋白表达，抑制TNF-α和IL-6的产生（P〈0．01）以及NF—KB和AP-1的激活。结论Syk抑制剂降低LPS诱导的巨噬细胞分泌NO和iNOS的蛋白表达及TNF-α和IL-6的释放，通过下调Syk下游TLR4受体信号传导通路的蛋白，进而显著降低转录因子NF-KB和AP-1的激活有关。

关键词： Syk抑制剂脂多糖 RAW264 7巨噬细胞抗炎作用

Schoolchildren from disadvantaged backgrounds present a loss of lean tissue mass and significant increase of body fat mass during the COVID-19 lockdown in Germany:results from the MEDdirect study

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World Journal of Pediatrics 2022年第5期18卷 363-367页

作者： David Muhmann Marvin Droste Jorn Schulz Frederike Linge Lea Ladewig ursula Eisenberg Susanne Roder Rainer Buscher University Hospital Essen PediatricsⅡHufelandstr.5545147 EssenGermany Be Strong for Kids E.V Sundernholz 10845134 EssenGermany Schule Am Steeler Tor Engelbertstr.445127 EssenGermany

Children from socio-economically disadvantaged backgrounds receive unequal opportunities to succeed in school,which will lead to a decreased health literacy as adults[U].This is not only directly associated with an impaired eco-nomic and mental health and wellbeing of young people,but above all with an in creased adult mortality[1-4].While this problem is well known and described,only few and even fewer pediatric studies suggest how to interrupt this downward spiral consistently[5,6].

关键词： disadvantage adult impaired

CLOFILIUM, A POTENT BLOCKER OF SODIUM AND CALCIUM CHANNELS IN GUINEA-PIG VENTRICULAR MYOCYTES

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Chinese Medical Journal 1995年第3期 74-75页

作者： Qi Li Herbert M Himmel ursula Ravens Institut fur Pharmakologie Universitat Essen Essen GermanyInstitut fur Pharmakologie Universitat Essen Essen GermanyInstitut fur Pharmakologie Universitat Essen Essen Germany

The unsatisfactory results of clinical trials with class-Ⅰ antiarrhythmic drugs have prompted a renaissance of interest in compounds with class-Ⅲ antiarrhythmic action. In the present, we have reevaluated the class-... 详细信息

关键词： A POTENT BLOCKER OF SODIUM AND CALCIUM CHANNELS IN GUINEA-PIG VENTRICULAR MYOCYTES CLOFILIUM

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Bodies as evidence

作者： Mark Maguire ursula Rao and Nils Zurawski editors.

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FAMILY TIES

城市漫步（全国英文版） 2015年第2期 34-35页

作者： ursula Kay 不详

When David and I came to China back in 2011,we married as a couple ready to make some big bucks and start a new *** babies was not our plan,but we never really plan things anyway;we either do things or we don't-and we... 详细信息

关键词： really things talking

Specifying the role of BAK1-interacting receptor-like kinase 3 in brassinosteroid signaling

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Journal of Integrative Plant Biology 2020年第4期62卷 456-469页

作者： Ruth Groβeholz Anna Feldman-Salit Friederike Wanke Sarina Schulze Nina Glockner Birgit Kemmerling Klaus Harter ursula Kummer Centre for Organismal Studies/Bio Quant Heidelberg University69120 HeidelbergGermany Center for Plant Molecular Biology(ZMBP) University Tübingen72076 TubingenGermany

Brassinosteroids(BR) are involved in the control of several developmental processes ranging from root elongation to senescence and adaptation to environmental cues. Thus, BR perception and signaling have to be precisely regulated. One regulator is BRI1-associated kinase 1(BAK1)-interacting receptor-like kinase 3(BIR3). In the absence of BR, BIR3 forms complexes with BR insensitive 1(BRI1) and ***, the biophysical and energetic requirements for complex formation in the absence of the ligand have yet to be determined. Using computational modeling, we simulated the potential complexes between the cytoplasmic domains of BAK1, BRI1 and BIR3. Our calculations and experimental data confirm the interaction of BIR3 Rewith BAK1 and BRI1, with the BAK1 BIR3 interaction clearly favored. Furthermore, we demonstrate that BIR3 and BRI1 share the same interaction site with BAK1. This suggests a competition between BIR3 and BRI1 for binding to BAK1, which results in preferential binding of BIR3 to BAK1 in the absence of the ligand thereby preventing the active participation of BAK1 in BR signaling. Our model also suggests that BAK1 and BRI1 can interact even while BAK1 is in complex with BIR3 at an additional binding site of BAK1 that does not allow active BR signaling.

关键词： BAK1 interaction interacting

Argument and *** Connectors in the History of English 1st

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Impairment of the Endothelium and Disorder of Microcirculation in Humans and Animals Exposed to Infrasound due to Irregular Mechano-Transduction

丛书名： Topics in English linguistics

2010年

作者： ursula Lenker

ISBN: (数字)9783110216066

ISBN: (纸本)9783110205589

The book is the first corpus-based study giving a comprehensive overview of English items which have been used as adverbial connectors ('conjuncts', 'linking adverbials'), from Old English to Present-Day English. The author analyses different characteristics of the make-up, functions and use of connectives, and considers morphological and syntactic factors as well as pragmatic, textlinguistic and

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Journal of Biosciences and Medicines 2023年第6期11卷 30-56页

作者： ursula Maria Bellut-Staeck Independent Scientist Berlin Germany

The microcirculation of mammals is an autoregulated and complex synchronised system according to the current demand for nutrients and oxygen. The undisturbed course of vital functions such as of growth, blood pressure regulation, inflammatory sequence and embryogenesis is bound to endothelial integrity. The sensible vasomotion is particularly dependent on it. Mechano-transduction signalling networks play a critical role in vital cellular processes and are the decisive physiological mechanism for an adequate NO-release, main responsible for the autoregulation of vessels. Disturbed endothelial integrity, originating, e.g., from chronic oxidative stress and/or mechanic (oscillatory) stress, leads to disturbance of vasomotion as well as a disequilibrium of redox systems, recognized as main cause for the development of chronic inflammation diseases such as atherosclerosis and corresponding secondary illnesses, possibly cancer. The endothelial cytoskeleton, which corresponds to a viscoelastic “tensegrity model”, offers the possibility for mechano-transduction via its special construction. The rapidly growing knowledge about mechanical forces in cellular sensing and regulation of the last years (that culminated in the Nobel Prize award for the decoding of pressure/vibration sensing ion channels), led us to the following hypothesis: The extern stressor “Noise” produces under certain conditions an oscillatory stress field in the physiologically laminar flow bed of capillaries, which is able to lead to irregular mechano-transductions. Findings provide a strict dependence on frequency in mechano-transduction with determination of thresholds for a 1:1 transmission. The knowledge, recently gained on endothelial mechano-transduction, sheds a new light on the importance of low frequencies. This could indicate the long-sought pathophysiological way in which infrasound can exert a stressor effect at the cellular level. Noise-exposed citizens, who live near infrastructures such as a

关键词： Mechano-Transduction Endothelial Cytoskeleton Infrasound Oscillatory Stress Vasomotion

Xenobiotic effects in cancer related pathways-high throughput screening and proof of concept in animal models

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中国比较医学杂志 2013年第1期23卷 69-80页

作者： ursula E. Schumacher Suzhou Xishan Zhongke Drug Research & Development Co. Ltd. 1336 Wuzhong AvenueSuzhou 215104JiangsuChina

Xenobiotic drugs and chemicals directly interact with DNA,proteins,or other biomolecules in cells. These direct interactions with molecular targets may trigger a series of downstream effects on metabolic-biochemical and regulatory-signaling networks that can invoke cellular consequences leading to adaptive homeostatic or adverse pathological responses. Regulators for drug and chemicals safety have therefore since long required the testing of toxicity in animal models before drugs and pesticides can enter the market. The US National Research Council of the National Academy of Sciences,in its report,Toxicity Testing in the 21st Century: a Vision and a Strategy [1] ,proposed that toxicity testing should become less reliant on apical endpoints from whole animal tests and eventually rely instead on quantitative,doseresponse models based on information from in vitro assays and in vivo biomarkers,which can be used to screen large numbers of chemicals. The present paper reports about a combination of HTS in vitro assays that can be used to study the potential tumorigenic effect of xenobiotics ( drug targets,environmental chemicals) via a set of"sentinel"genes [2] that are functionally interrelated based on evidence weighted functional linkage network ( FLN ) log-likelihood scores ( Linghu et al [3] ) .

关键词：基础医学呼吸生理呼吸运动人体生理学