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Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

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World Journal of Gastroenterology 2001年第3期7卷 381-386页

作者： Jun Hui Cui~1 uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General surgery,First Affiliated Hospital,College of medicine,Zhejiang university,Hangzhou 310003,Zhejiang Province,China ~2Department of General surgery,Christian-Albrechts-university,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang medical university in 1984,earned master degree in 1990,studied in the surgical Department of Kiel university and worked in the Lab of molecular Oncology of Kiel university from 1994-1997achieved m.D.from Kiel university.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. Department of General surgery First Affiliated Hospital College of Medicine Zhejiang University 79 Qingchun Road Hangzhou 310003 Zhejiang ProvinceChina. Cjhui@***

AIm To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer *** Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PAsstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific *** There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific *** An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticme

关键词： mice, nude gastrointestinal neoplasms/pathology neoplasm seeding neoplasm metastasis

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穴位刺血治疗常年性变应性鼻炎疗效观察

时珍国医国药 2001年第8期12卷 714-715页

作者：陈永红吕琳陈红李凤珍谢爱泽黄玉艳广西民族医药研究所附属医院广西南宁530001 广西民族医药研究所临床研究室广西南宁530001

目的 :探讨穴位刺血不同处方用穴治疗常年性变应性鼻炎的临床疗效。方法 :12 3例常年性变应性鼻炎患者随机分为鼻通组穴 (3 2例 )、下迎香组穴 (3 1例 )、通天组穴 (3 0例 )穴位刺血治疗组 ,并设立息斯敏治疗对照 (3 0例 )。观察穴位刺... 详细信息

目的 :探讨穴位刺血不同处方用穴治疗常年性变应性鼻炎的临床疗效。方法 :12 3例常年性变应性鼻炎患者随机分为鼻通组穴 (3 2例 )、下迎香组穴 (3 1例 )、通天组穴 (3 0例 )穴位刺血治疗组 ,并设立息斯敏治疗对照 (3 0例 )。观察穴位刺血不同处方用穴治疗前后症状及体征的变化 ,评定各组的疗效。结果 :鼻通组穴组、下迎香组穴组、通天组穴组近期疗效总有效率分别为87.5 0 % ,83 .87% ,83 .3 3 % ;远期疗效总有效率分别为 5 6.67% ,5 1.72 % ,5 5 .17%。息斯敏治疗对照组总有效率为 93 .3 3 %。组间比较差异无统计学意义 ,P>0 .0 5。结论

关键词：穴位刺血变应性鼻炎常年性治疗

注射微泡对比剂后的时间-强度曲线判断克罗恩病病人对抗炎药物有无应答的价值

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国际医学放射学杂志 2013年第4期 405-405页

作者： E. Quaia B. Cabibbo L.D. Paoli W. Toscano G. Poillucci m. Cova 毛子婧 Department of Radiology Cattinara Hospital University of TriesteStrada di Fiume 44734149 Trieste Italy

目的探讨注射六氟化硫微泡对比剂获得的时间-强度曲线判断克罗恩病(CD)病人有无应答的价值。方法纳入43例初始诊断为活动性CD病人[男29例,女14例;平均年龄(48.5±17.17)岁]。对每例病人于药物治疗开始后12周评估治疗结果。注射六氟化硫... 详细信息

目的探讨注射六氟化硫微泡对比剂获得的时间-强度曲线判断克罗恩病(CD)病人有无应答的价值。方法纳入43例初始诊断为活动性CD病人[男29例,女14例;平均年龄(48.5±17.17)岁]。对每例病人于药物治疗开始后12周评估治疗结果。注射六氟化硫微泡后扫描末端回肠袢,首过动态强化的数字电影片段以灰阶形式定量。比较应答和无应答病人的最大强化百分比、达峰时间以及时间-强度曲线下面积,应用mann-Whitney u非参数检验。结果应答者(n=25)和无应答者(n=18)比较,时间-强度曲线下面积[(621.58±374.53)∶(1199.64±386.39),P<0.05]存在差异,最大增强百分比[(41.26±15.22)∶(43.17±4.41),P=0.25]和达峰时间[(11.31±3.06)∶(10.12±3.47),P=0.15]无差异。结论注射微泡后获得的时间-强度曲线下面积是唯一能判断CD病人对药物治疗有无应答的参数。要点①使用微泡对比剂的动态超声有助于评估炎症性肠病。②时间-强度曲线可以评估CD的治疗效果。③时间-强度曲线下面积可用以判断病人对药物治疗有无应答。

关键词：克罗恩大肠超声对比微泡

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长期摄入咖啡因与女性高血压患病风险

世界核心医学期刊文摘(心脏病学分册) 2006年第4期 20-20页

作者： Winkelmayer W. C. stampfer m. J. Willett W. C. Curhan G. C. 任付先 Division of Pharmacoepidemiology and Pharmacoeconomics Renal Division Brigham and Women's Hospital 1620 Tremont St BostonMA 02120 United States Dr.

Context: Caffeine acutely increases blood pressure, but the association between habitual consumption of caffeinated beverages and incident hypertension is uncertain. Objective: To examine the association between caffeine intake and incident hypertension in women. Design, setting, and Participants: Prospective cohort study conducted in the Nurses'Health studies(NHss)I and II of 155 594 us women free from physician-diagnosed hypertension followed up over 12 years(1990-1991 to 2002-2003 questionnaires). Caffeine intake and possible confounders were as certained from regularly administered questionnaires. We also tested the associations with types of caffeinated beverages. main Outcome measure: Incident physician-diagnosed hypertension. Results: During follow-up, 19 541 incident cases of physician-diagnosed hypertension were reported in NHs I and 13 536 in NHs II. In both cohorts, no linear association between caffeine consumption and risk of incident hypertension was observed after multivariate adjustment(NHs I, P for trend=.29; NHs II, P for trend=.53). using categorical analysis, an inverse u-shaped association between caffeine consumption and incident hypertension was found. Compared with participants in the lowest quintile of caffeine consumption, those in the third quintile had a 13%and 12%increased risk of hypertension, respectively(95%confidence interval in NHs I, 8%-18%; in NHs II, 6%-18%). Whe n studying individual classes of caffeinated beverages, habitual coffee consumption was not associated with increased risk of hypertension. By contrast, consumption of cola beverages was associated with an increased risk of hypertension, independent of whether it was sugared or diet cola(P for trendusion: No linear association between caffeine consumption and incident hypertension was *** though habitual coffee consumption was not associated with an increased risk of hypertension, consumption of sugared or diet cola was associated with it. Further re

关键词：高血压饮料患病风险咖啡因嘌呤生物碱女性

彩色多普勒超声技术测定正常中期妊娠血流的研究

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同济医科大学学报 2000年第3期29卷 246-248页

作者：苏晓文穆罕默德孙永玉丁玉莲同济医科大学附属协和医院妇产科武汉430022

采用彩色多普勒超声技术 (CDFI)测定 312例正常中期妊娠孕妇脐动脉 (uA)、胎儿大脑中动脉 (mCA)、胎儿腹主动脉 (AA)及子宫动脉 (u TA)的收缩期峰值速度 (s)、舒张末期速度 (D)、s/D比值、阻力指数 (RI)和搏动指数 (PI)。结果显示 mCA... 详细信息

采用彩色多普勒超声技术 (CDFI)测定 312例正常中期妊娠孕妇脐动脉 (uA)、胎儿大脑中动脉 (mCA)、胎儿腹主动脉 (AA)及子宫动脉 (u TA)的收缩期峰值速度 (s)、舒张末期速度 (D)、s/D比值、阻力指数 (RI)和搏动指数 (PI)。结果显示 mCA的检出较其它血管困难 ,但随孕周而检出递增。多数动脉的速度参数随孕周而升高 ,阻力参数随孕周而递减 ,与孕周相关关系有显著意义 (PmCA中 D随孕周变化不明显 (P>0 .0 5 ) ,其阻力值随孕周有升高倾向。逐步回归分析显示 u A的 D和 s/D、mCA的 s和 RI、AA的 s及 uTA的 RI等参数与孕周关系密切。因此认为正常中期妊娠的子宫、胎盘及胎儿血流动力学有其变化规律。

关键词：中期妊娠多普勒超声血流参数测定

CaBa-铝酸盐玻璃中Cr^(4+)谱线宽度随温度的变化

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发光学报 2000年第3期21卷 185-189页

作者：黄世华鄂书林王有贵张家骅吴兴坤严懋勋中国科学院激发态物理开放研究实验室吉林长春130021 北京师范大学系统科学系北京100875 佐治亚大学物理和天文系

测量了 1 5～ 3 0 0K温度范围内 57 5%Al1 5O 3 5%CaO 7 5%BaO玻璃中四价铬的发射光谱。这种材料中铬离子的能级处于Tanabe sugano图上弱场范围中 ,最低的激发态是3 T2 ,发射谱是一个宽带。按照单频近似理论拟合低温下的光谱 ,得到3 T2... 详细信息

测量了 1 5～ 3 0 0K温度范围内 57 5%Al1 5O 3 5%CaO 7 5%BaO玻璃中四价铬的发射光谱。这种材料中铬离子的能级处于Tanabe sugano图上弱场范围中 ,最低的激发态是3 T2 ,发射谱是一个宽带。按照单频近似理论拟合低温下的光谱 ,得到3 T2 能级的零声子线位置Ezp=84 0 0cm-1,声子能量 ω =3 2 0cm-1,黄昆因子s =3 58。尽管单频近似能够较好地描述低温下的线形 ,发射光谱宽度随温度的变化却与单频近似理论的结果不符。讨论了这种差别的原因 ,认为可能的解释是与激发态耦合的声子能量大于与基态耦合的声子能量。

关键词： CABA-铝酸盐玻璃中铬离子光谱谱线宽度温度

43763名美国放射科医师和64990名精神科医师的长期死亡率的对比研究

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国际医学放射学杂志 2017年第1期 95-95页

作者： A.B.de González E.Ntowe C.m.Kitahara E.Gilbert D.L.miller R.A.Kleinerman 高云云 Radiation Epidemiology Branch Division of Cancer Epidemiology and Genetics National Cancer Institute

摘要目的比较放射科医师和精神科医师总死亡率、特殊病种死亡率、总恶性肿瘤死亡率、特殊恶性肿瘤死亡率的差异,是否和已知辐射暴露风险以及随着时间推移辐射暴露

关键词：放射科医师 RR 精神科医师死亡率美国美利坚合众国北美洲

Plant Biosystems Design Research Roadmap 1.0

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BioDesign Research 2020年第1期2020卷 53-90页

作者： Xiaohan Yang June I.medford Kasey markel Patrick m.shih Henrique C.De Paoli Cong T.Trinh Alistair J.mcCormick Raphael Ployet steven G.Hussey Alexander A.myburg Poul Erik Jensen md mahmudul Hassan Jin Zhang Wellington muchero udaya C.Kalluri Hengfu Yin Renying Zhuo Paul E.Abraham Jin-Gui Chen David J.Weston Yinong Yang Degao Liu Yi Li Jessy Labbe Bing Yang Jun Hyung Lee Robert W.Cottingham stanton martin mengzhu Lu Timothy J.Tschaplinski Guoliang Yuan Haiwei Lu Priya Ranjan Julie C.mitchell stan D.Wullschleger Gerald A.Tuskan Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innovation Oak Ridge National LaboratoryOak RidgeTN 37831USA Department of Biology Colorado State UniversityFort CollinsCO 80523USA Department of Plant Biology University of CaliforniaDavisDavisCAUSA Feedstocks Division Joint BioEnergy InstituteEmeryvilleCAUSA Department of Biodesign Biological Systems and Engineering DivisionLawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering University of TennesseeKnoxvilleTN 37996USA synthsys and Institute of molecular Plant sciences School of Biological SciencesUniversity of EdinburghEdinburgh EH93BFUK Department of Biochemistry Genetics and MicrobiologyForestry and Agricultural Biotechnology Institute(FABI)University of PretoriaPretoria 0002South Africa Department of Food science University of CopenhagenRolighedsvej 26DK-1858FrederiksbergCopenhagenDenmark state Key Laboratory of subtropical silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiang 311300China state Key Laboratory of Tree Genetics and Breeding Research Institute of Subtropical ForestryChinese Academy of ForestryHangzhouZhejiang 311400China Department of Plant Pathology and Environmental microbiology and the Huck Institute of the Life sciences The Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Genetics Cell Biology and DevelopmentCenter for Precision Plant Genomics and Center for Genome EngineeringUniversity of MinnesotaSaint PaulMN 55108USA Department of Plant science and Landscape Architecture University of ConnecticutStorrsCT 06269USA Division of Plant sciences Bond Life Sciences CenterUniversity of MissouriColumbiaMOUSA Donald Danforth Plant science Center St.LouisMOUSA Environmental sciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA

Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable *** plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design *** represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological *** biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant *** this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology *** highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of ***,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance.

关键词： Plant utilizing breeding

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昆明种小鼠ＤＮＡ指纹图

北京实验动物科学 1993年第3期10卷 5-8页

作者：杜国清陈兆平四川省医学院医学实验动物中心

无菌取昆明小鼠肝脏提取DNA,用HacⅢ和Hin1酶切,1%琼脂糖凝胶电泳,然后进行sotuthern转移,将分离得到的DNA片段转移到尼龙膜上,分别用人体多核小卫星33.15和噬菌体m13作探针与DNA片段杂交。当用33.15探针杂交时,得到其DNA指纹图,DNA指... 详细信息

无菌取昆明小鼠肝脏提取DNA,用HacⅢ和Hin1酶切,1%琼脂糖凝胶电泳,然后进行sotuthern转移,将分离得到的DNA片段转移到尼龙膜上,分别用人体多核小卫星33.15和噬菌体m13作探针与DNA片段杂交。当用33.15探针杂交时,得到其DNA指纹图,DNA指纹图平均相似系数(F)Hac酶切时F为0.592±0.014,Hinf1酶切时F为0.583±0.013.说明该实验组小鼠来自同一群体内,DNA指纹图相似系数已超过一级亲缘关系个体间的相似系数。同时表明昆明小鼠基因组中存在着大量与人体小卫星同源的小卫星区。用33.15探针时获得了昆明种小鼠的DNA指纹图。用噬菌体m13作探针未得到清晰的DNA指纹图,有待于进一步探索。

关键词： DNA指纹图实验动物