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Intracellular in situ labeling of Ti02 nanoparticles for fluorescence microscopy detection

Nano Research 2018年第1期11卷 464-476页

作者： koshonna Brown Ted Thurn Lun Xin William Liu Remon Bazak Si Chen barry Lai Stefan Vogt Chris Jacobsen Tatjana Paunesku Gayle E. Woloschak Department of Radiation Oncology Feinberg School of Medicine Northwestern University Chicago Illinois 60611 USA X-ray Science Division Advanced Photon Source Argonne National Laboratory 9700 South Cass Avenue Argonne Illinois 60439 USA Department of Physics & Astronomy Weinberg College of Arts and Sciences 2145 Sheridan Road Evanston Illinois 60208 USA

Titanium dioxide （TiO2） nanoparticles are produced for many different purposes, including development of therapeutic and diagnostic nanoparticles for cancer detection and treatment, drug delivery, induction of DNA double-strand breaks, and imaging of specific cells and subcellular structures. Currently, the use of optical microscopy, an imaging technique most accessible to biology and medical patholog36 to detect TiO2 nanoparticles in cells and tissues ex vivo is limited with low detection limits, while more sensitive imaging methods （transmission electron microscopy, X-ray fluorescence microscop~ etc.） have low throughput and technical and operational complications. Herein, we describe two in situ post- treatment labeling approaches to stain TiO2 nanoparticles taken up by the cells. The first approach utilizes fluorescent biotin and fluorescent streptavidin to label the nanoparticles before and after cellular uptake; the second approach is based on the copper-catalyzed azide-alkyne cycloaddition, the so-called Click chemistry, for labeling and detection of azide-conjugated TiO2 nanoparticles with alkyne- conjugated fluorescent dyes such as Alexa Fluor 488. To confirm that optical fluorescence signals of these nanoparticles match the distribution of the Ti element, we used synchrotron X-ray fluorescence microscopy （XFM） at the Advanced Photon Source at Argonne National Laboratory. Titanium-specific XFM showed excellent overlap with the location of optical fluorescence detected by confocal microscopy. Therefore, future experiments with TiO2 nanoparticles may safely rely on confocal microscopy after in situ nanoparticle labeling using approaches described here.

关键词： TiO2 nanoparticles anatase Click reaction biotin-streptavidin synchrotron X-ray fluorescence microscopy

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Efficacy of IABP in patients awaiting cardiac transplantation

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医学研究生学报 1991年第1期4卷 78-81页

作者： barry F.uretsky Division of Cardiology and Cardiothoracic Surgery Presbyterian-University Hospital and the University of PittsburghPittsburghPA 15213 U.S.A

Intra-aortic balloon pump (IABP)support is used in severalclinical *** determine its efficacy in patients with severe heartfailure awaiting cardiac transplantation,the experience at the universityof Pittsburgh from May 1986 to October 1989 was *** 392 patientson the waiting list,(16.3%) required *** had previously been oninotropic support for varying periods of *** ability to stabilize andto sustain the patient to cardiac transplantation was 75%.Twenty patients(31.3% of group) required and were provided more extensive mechanicalzsupport with either total artificial heart (n=ll) or left ventrieular assistdevice (n =9).Twenty-nine patients (45% of entire gronp) were able tohave the IABP removed prior to *** patients(26.6%)parameters demonstrated improvement with *** data support IABPas a bridge to transplantation in patients with severe heart failure who havenot been stabilized on inotropie agents *** data also demonstratethat IABP support may only be required temporarily in a subgroup of pa-tients awaiting cardiac transplantation.

关键词： Intra-aortic balloon pump(IABP) cardiac transplantation

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晚白垩世以来川东北地区的剥蚀历史--多类低温热年代学数据综合剖面的制约

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地球物理学报 2011年第3期54卷 807-816页

作者：田云涛朱传庆徐明饶松 barry P．kohn 胡圣标中国科学院地质与地球物理研究所岩石圈演化国家重点实验室北京100029 澳大利亚墨尔本大学地球科学学院维多利亚3010

低温热年代学数据是一个与热历史过程紧密相关的资料类型,与高温年代学不同,低温热年代学表观年龄本身在很多情况下没有直接的地质意义.当且仅当样品线性持续冷却的情况下,表观年龄才可以被直接解释为样品经过其封闭温度的大致时间.因此... 详细信息

低温热年代学数据是一个与热历史过程紧密相关的资料类型,与高温年代学不同,低温热年代学表观年龄本身在很多情况下没有直接的地质意义.当且仅当样品线性持续冷却的情况下,表观年龄才可以被直接解释为样品经过其封闭温度的大致时间.因此,只有结合地质约束通过对低温热年代学数据进行热历史模拟才能更好地揭示其所蕴含的地质信息.对川东北地区现有裂变径迹数据的统计显示,露头样品的表观年龄主要集中在60～80 Ma,其为冷却年龄并无直接的地质意义.前人利用这些数据对川东北地区热历史进行了模拟,然而不同研究者的研究结果却不甚一致,争议主要集中在四川盆地最后一期剥蚀开始的时间上.这体现了单一低温热年代学指标应用范围局限的缺陷.为解决这一问题,本文介绍了一种多类低温热年代学数据剖面联合解释的方法:首先根据低温热年代学动力学模型对诸多种可能的热历史进行正演模拟,然后将正演模拟的结果与观测结果相比较,因此,通过对比正演模拟结果与实测结果的拟合程度便可从诸多种可能的热历史中选择出最可能的一种.本文利用此方法对四川盆地东北部已发表的诸多可能的冷却/剥蚀历史进行了正演模拟,并将这些正演模拟与实测磷灰石裂变径迹和(u-Th)/He综合深度剖面数据进行比较,更好地制约了四川盆地的热历史:～100 Ma和～30 Ma之间冷却速度为0.57℃/Ma,～30 Ma以来冷却速度加快(～1.67℃/Ma).在假设川东北地区100Ma内地温梯度大致与现今20℃/km的地温梯度相近的前提下,其剥蚀历史可计算为:在～100 Ma和～30 Ma之间剥蚀速度为29 m/Ma,～30 Ma以来剥蚀速度加快(～83 m/Ma),川东北地区自晚白垩世以来总剥蚀量约为5 km.

关键词：低温热年代学热历史模拟剥蚀川东北地区

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IMPROVEMENTS TO THE PASSIVE LOSSLESS SNuBBERS FOR POWER BRIDGE LEGS

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Journal of Electronics(China) 2001年第3期18卷 260-266页

作者： barry W. Williams Stephen J. Finney Dept. of Computing & Electrical Engineering Heriot-Watt University Edinburgh EH14 4AS. UKDept. of Computing & Electrical Engineering Heriot-Watt University Edinburgh EH14 4AS. UK

The paper considers three common snubber circuits used on gate turn-off thyristor and/or insulated gate bipolar transistor inverters. The three snubbers are passive lossless circuits for power bridge legs, and the improvements and modifications to these snubber circuits are presented. The comparative features and operation of the three improved energy recovery snubbers are discussed and supported by PSPICE simulations and experimental results.

关键词： Snubber Inverter Thyristor Transistor

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LOCAL EFFECTS OF ACuPuNCTuRE ON THE MEDIAN AND uLNAR NERVES IN PATIENTS WITH CARPAL TuNNEL SYNDROME:STuDY DESIGN AND PRELIMINARY RESuLTS

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Journal of Integrative Medicine 2014年第3期 256-257页

作者： Alexandara Dimitrova Jau-Shin Lou Yunpeng Luo barry Oken Oregon Health and Science university

Objective: The purpose of this mechanistic study is to use electrophysiologic measurements, nerve conduction studies(NCS) and quantitative sensory testing(QST), to assess the local, nerve-specific effect of 3 acupuncture modalities on two anatomically distinct nerves in the forearm(median and ulnar nerve) in subjects with carpal tunnel syndrome(CTS).We expect to see a greater response in the diseased nerve(median), compared to the healthy one(ulnar).Methods: Participants with CTS were randomized to 3 intervention groups: manual, low- and high-frequency *** week 1, baseline NCS and QST measurements were obtained in both nerve territories, followed by acupuncture to the median nerve(pericardium channel points) and to the ulnar nerve(heart channel points); one week apart, order *** was followed by repeated NCS and QST measurements for both *** week 2, repeated baseline QST and NCS measurements were obtained, followed by acupuncture to the nerve which was not treated in week 1, and by post-acupuncture NCS and QST measurements to both ***: Three pilot participants have been tested to *** pilot 1, acupuncture caused 66% improvement of the median cold detection threshold, but not in the healthy ulnar nerve, with corresponding 60% increase in median compound muscle action potential(CMAP) *** pilot 2, Median Sensory Nerve Action Potentials(SNAP) after acupuncturing, which had been missing at baseline due to CTS, was *** pilot 3, acupuncture caused improvement in median nerve cold(33%)and vibration(55%) sensation, with corresponding increases in median motor and sensory nerve conduction velocity(NCV).ulnar sensory NCV and SNAP amplitude also increased with *** 3 subjects reported symptomatic ***: Acupuncture appears to have nerve-specific effect on the median and ulnar nerves in the forearm, which can be measured by NCS and *** effects will be fully assessed u

关键词： QST NCS CTS LOCAL EFFECTS OF ACuPuNCTuRE ON THE MEDIAN AND uLNAR NERVES IN PATIENTS WITH CARPAL TuNNEL SYNDROME

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Resolving the Mortierellaceae phylogeny through synthesis of multi-gene phylogenetics and phylogenomics

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Fungal Diversity 2020年第5期104卷 267-289页

作者： Natalie Vandepol Julian Liber Alessandro Desirò Hyunsoo Na Megan kennedy kerrie barry Igor V.Grigoriev Andrew N.Miller kerry O’Donnell Jason E.Stajich Gregory Bonito Department of Microbiology and Molecular Genetics Michigan State UniversityEast LansingMI 48824USA Department of Plant Biology Michigan State UniversityEast LansingMI 48824USA Department of Plant Soil and Microbial Sciences Michigan State UniversityEast LansingMI 48824USA Joint Genome Institute BerkeleyCA 94720USA Illinois Natural History Survey University of Illinois Urbana-ChampaignChampaignIL 61820USA united States Department of Agriculture Agricultural Research ServicePeoriaIL 61604USA Department of Microbiology and Plant Pathology&Institute for Integrative Genome Biology University of California-RiversideRiversideCA 92521USA

Early efforts to classify Mortierellaceae were based on macro-and micromorphology,but sequencing and phylogenetic studies with ribosomal DNA(rDNA)markers have demonstrated conflicting taxonomic groupings and polyphyletic *** some taxonomic confusion in the family has been clarified,rDNA data alone is unable to resolve higher level phylogenetic relationships within *** this study,we applied two parallel approaches to resolve the Mortierel-laceae phylogeny:low coverage genome(LCG)sequencing and high-throughput,multiplexed targeted amplicon sequenc-ing to generate sequence data for multi-gene *** then combined our datasets to provide a well-supported genome-based phylogeny having broad sampling depth from the amplicon *** the Mortierellaceae phylogeny into monophyletic genera resulted in 13 genera,7 of which are newly ***-coverage genome sequencing proved to be a relatively cost-effective means of generating a high-confidence *** multi-gene phylogenetics approach enabled much greater sampling depth and breadth than the LCG approach,but has limitations *** present this work to resolve some of the taxonomic confusion and provide a genus-level framework to empower future studies on Mortierellaceae diversity and evolution.

关键词： Mortierellaceae Phylogenomics Molecular systematics Taxonomy Multi-locus sequence typing

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Metabolic alterations and the potential for targeting metabolic pathways in the treatment of multiple myeloma

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Journal of Cancer Metastasis and Treatment 2019年第4期4卷 1-16页

作者： Dustin Rizzieri barry Paul Yubin kang Division of Hematological Malignancies and Cellular Therapy Duke University Medical Center Durham NC 27710 USA

Metabolism is defined as the collection of complex biochemical processes that living cells use to generate energy and maintain their growth and survival. Metabolism encompasses the synthesis and breakdown of glucose, fatty acids, and amino acids;the generation of energy (ATP);and oxidative phosphorylation. In cancer cells, metabolism can be commandeered to promote tumor growth and cellular proliferation. These alterations in metabolism have emerged as an additional hallmark of various cancers. In this review we focus on metabolic alterations in multiple myeloma (MM) - a malignancy of plasma cells - including derangements in glycolysis, gluconeogenesis, the tricarboxylic acid cycle, oxidative phosphorylation, and fatty acid/amino acid synthesis and degradation. Particular focus is given to metabolic alterations that contribute to myeloma cell growth, proliferation and drug resistance. Finally, novel approaches that target metabolic pathways for the treatment of MM are discussed.

关键词： Metabolism alterations multiple myeloma treatment

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“积极应对全球化与国际经济秩序转型风险”笔谈

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东北亚论坛 2019年第5期28卷 48-63,127,128页

作者：巴里·艾肯格林侯力边卫红郝毅王皓(译) 美国加州大学伯克利分校吉林大学东北亚研究中心中国银行国际金融研究所中国银行博士后工作站中国人民大学博士后流动站吉林大学中国国有经济研究中心吉林大学经济学院意大利罗马大学

近年来,党中央高度重视风险防范问题。党的十九大报告将防范化解重大风险与精准脱贫、污染防治并称为“三大攻坚战”。2019年1月,习近平总书记在省部级主要领导干部坚持底线思维着力防范化解重大风险专题研讨班开班式上发表重要讲话,对... 详细信息

近年来,党中央高度重视风险防范问题。党的十九大报告将防范化解重大风险与精准脱贫、污染防治并称为“三大攻坚战”。2019年1月,习近平总书记在省部级主要领导干部坚持底线思维着力防范化解重大风险专题研讨班开班式上发表重要讲话,对我国当前防范化解重大风险工作作出系统部署,明确提出要“深刻认识和准确把握外部环境的深刻变化和我国改革发展稳定面临的新情况新问题新挑战,坚持底线思维,增强忧患意识,提高防控能力”。我国经济已经深度融入全球生产网络和经济体系,国际环境的重大变化必将深刻影响经济运行。因此,防范风险尤其需要注意国际环境变化风险。本刊从国内外学者来稿中择发三篇有代表性的研究成果,分别阐述了金融危机防范、国际经济秩序危机应对、主权债务危机处理三个重大问题,希望以此推动关于风险防范问题的学术研究。

关键词：国际经济秩序全球生产网络主权债务危机精准脱贫危机应对防范风险党的十九大报告风险防范

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中美大对比:在哪儿幸福更容易?

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疯狂英语（阅读版） 2013年第10期 23-25页

作者： barry Cunningham Leonard

在中国涌现“出国热”的那几年，我们的父辈以及我们都在向往着国外的福利、教育和生活环境。有时候梦见自己身处国外都会是一件乐事，值得人们茶余饭后聊上半天。长大以后，随着国外的YouTube、Twitter和国内各类视频网站以及微博客的... 详细信息

在中国涌现“出国热”的那几年，我们的父辈以及我们都在向往着国外的福利、教育和生活环境。有时候梦见自己身处国外都会是一件乐事，值得人们茶余饭后聊上半天。长大以后，随着国外的YouTube、Twitter和国内各类视频网站以及微博客的兴起，我们越来越多地了解到国外也有贫民窟、国外也有经济危机和失业、国外也有腐败和民主上的不完善，而更可怕的是．国外还有允许携带私人枪支的国民……

关键词： YouTube Twitter 幸福对比中美生活环境视频网站经济危机

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Genome-Wide Sequencing of 41 Rice （Oryza sativa L.） Mutated Lines Reveals Diverse Mutations Induced by Fast-Neutron Irradiation

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Molecular Plant 2016年第7期9卷 1078-1081页

作者： Li, Guotian Chern, Mawsheng Jain, Rashmi Martin, Joel A. Schackwitz, Wendy S. Jiang, Liangrong Vega-Sanchez, Miguel E. Lipzen, Anna M. barry, kerrie W. Schmutz, Jeremy Ronald, Pamela C. univ Calif Davis Dept Plant Pathol Davis CA 95616 USA univ Calif Davis Genome Ctr Davis CA 95616 USA Lawrence Berkeley Natl Lab Feedstocks Div Joint BioEnergy Inst Berkeley CA 94720 USA uS DOE Joint Genome Inst Walnut Creek CA 94598 USA Xiamen univ Sch Life Sci Xiamen 361102 Peoples R China HudsonAlpha Inst Biotechnol Huntsville AL 35806 USA Monsanto Co Chesterfield Village Campus Chesterfield MO 63017 USA

Fast-neutron （FN） irradiation has been used to create mutagen- ized collections of many plant species （Bolon et al., 2014）, FN- induced mutagenesis has clear advantages： it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis （Wang et al., 2013）. Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.

关键词： mutagenesis genome valuable

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