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World Journal of Gastroenterology 2007年第28期13卷 3873-3877页

作者： tze jui lam Benjamin CY Wong Chris JJ Mulder A Salvador Pea Wai Mo Hui Shiu Kum lam Annie On On Chan Department of Gastroenterology VU University Medical CentreAmsterdamThe Netherlands Department of Medicine University of Hong KongHong KongChina Department of Gastroenterology VU University Medical CentreAmsterdamThe Netherlands Department of PathologyVU University Medical CentreAmsterdamThe Netherlands

AIM： To investigate the distribution and frequency of advanced polyps over eight years.METHODS：6424 colonoscopies were reviewea aunng the study period 1998 to 2005. The study period was subdivided into period I ： 1998 to 2001 and period 11： 2002-2005. RESULTS： 1856 polyps （33% advanced polyps） and 328 CRCs were detected. The mean ages of the patients with advanced polyps and cancer were 69.2 ± 12.0 and 71.6± 13.8 years, respectively. Advanced polyps were mainly lerc sided （59.5%）. Advanced polyps were found in patients ≤60 years from 17.7% in period Ⅰto 26.3% in period Ⅱ（P 〈 0.05）, especially in male subjects ≤60 years （21.6% vs 31.6%, P 〈 0.05）. Advanced tubuloviUous polyps rose from 21.5% in period I to 29.5% in period Ⅱ （P 〈 0.05）. Whereas cancers in male patients ≤ 60 years were similar in both periods： 23.2% vs 16.5% （P 〉 0.05）.CONCLUSION： Advanced polyps increased significantly in the younger male group in the most recent period and there seems to be a shiE towards a proximal location.

关键词： Colonic polyps Colonoscopy Chinese

Inhibition of lysine methyltransferase G9a/GLP reinstates long-term synaptic plasticityand synaptic tagging/capture by facilitatingprotein synthesis in the hippocampal CA1area of APP/PS1 mouse model ofAlzheimer’s disease

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Translational Neurodegeneration 2021年第2期10卷 294-296页

作者： Javan Lee tze Han Karen Ka lam Pang Sheila Rui Xia Ang Mahima Sharma Sreedharan Sajikumar Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Neurobiology Programme Life Sciences InstituteNational University of SingaporeSingapore 117456Singapore Healthy Longevity Translational Research Programme Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117456Singapore

Histone H3 lysine 9 di-methylation(H3K9me2)is an epigenetic repressive histone modification that was found at aberrant states in Alzheimer’s disease(AD)patient samples[1].The addition of H3K9me2/3 is mainly catalyzed by lysine methyltransferase G9a,which functions as a heteromeric complex with G9a-like protein(GLP).G9a/GLP has other non-histone substrates,including itself.G9a/GLP is implicated in regulating synaptic plasticity,learning and memory[2,3].

关键词： Alzheimer GLP plasticity

Novel rapid tissue lysis method to evaluate cancer proteins: Correlation between elevated Bcl-X_L expression and colorectal cancer cell proliferation

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World Journal of Gastroenterology 2005年第33期11卷 5162-5168页

作者： Wei-Shone Chen Hong-Yi Chang jui-Ting Chang Jacqueline Ming Liu Chung-Pin Li Li-Li Chen Huei-Ling Chang Chia-Chi Chen tze-Sing Huang National Cancer Research Center National Health Research InstitutesTaipei Taiwan China Division of Colon and Rectal Surgery Department of Surgery Taipei Veterans General Hospital and National Yang-Ming University School of Medicine Taipei Taiwan China Division of Gastroenterology Department of Medicine Taipei Veterans General Hospital and National Yang-Ming University School of Medicine Taipei Taiwan China

AIM： We optimized a rapid and efficient tissue lysis method using the MagNA Lyser （Roche, Germany）. Using this novel method combined with immunoblot analysis, we investigated the correlation between abnormal Bcl-XL expression and clinicopathological characteristics in colorectal cancer. METHODS： Tissue samples from Sprague-Dawley rats were tested to determine optimal lysis conditions for use with MagNA Lyser. We next used the new method to extract tissue proteins from the tumor tissue of a colorectal cancer patient. The availability of extractable tissue proteins for proteomic study was demonstrated by two-dimensional （2D） gel electrophoresis and subsequent matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry. In addition, we prepared tissue lysates from paired tumor tissues and adjacent nontumor tissues of 50 colorectal carcinoma patients. Ensuing immunoblot analyses were performed to detect the level of Bcl-X, expression. RESULTS： The optimal sample sizes processed were found to be around 200 mg, with oscillation frequency of 6 500 r/rain for 80 s. Test of the first human tissue lysate confirmed that the MagNA Lyser method was adequate for protein extraction and subsequent identification by current proteomic protocols. The method was also applicable to immunoblot analysis. Thirty of 50 （60%） colorectal patients exhibited higher level of Bcl-XL expression in their tumor tissues. Raised level of Bcl-XL expression correlated with patients＇ gender and tumor cell proliferation index （P= 0.037 and P〈0.001, respectively）, but was independent of clinicopathological characteristics and overall survival. CONCLUSION： We report a novel tissue lysis method applicable to proteomic and immunoblot analyses, which can facilitate the discovery and detection of cancer protein alterations.

关键词： Tissue lysis MagNA Lyser Bcl-X, Colorectalcancer

Does <i>Culicoides</i>spp. (Diptera: Ceratopogonidae) Not Suck Human Blood in Riparian Habitat of a National Park

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Advances in Entomology 2017年第3期5卷 93-98页

作者： Ying-Hsi Lin Meng-Tieh Yu Tzu-Wen Kuo Ka-Iong lam Jeng-tze Yang Department of Medical Laboratory Science and Biotechnology Yuanpei University of Medical Technology Taiwan Department of Entomology National Chung Hsing University Taiwan Department of Entomology Agriculture and Forestry University Chitwan Nepal

Is the wild animal conservation result in Taiwan good enough to provide the blood resources for the blood sucking insect and cause the insect behavior changed? Based on the bimonthly investigation of Culicoides sp. in Wuling area, Sheipar National Park, by both human bait method and light trap method, we found that there were totally 13,191 individuals trapped by light trap and collected but no one single Culicoides sp. trapped by human bait during the study period of Wuling Long Term Ecological Research (WLTER) in 2012. Two of the three sites were recorded and reported as the midge attacked the ranger and tourists of Sheipa National Park in 2010 and 2011 respectively. Along with the study of WLTER some wild animals encounter more often than before, especially the bird population in the riparian ecosystem of the mountain river Chi Chia Wan Stream. On the other end, Culicoides sp. preferred to the blood of birds and amphibians as well as mammals according to the article review. So, the effectiveness of blood resource from wild animals may cause the behavior of blood sucking insect like as Culicoides sp. shifted to or returned back to the original and the favorite blood resource.

关键词： Culicoides Human Bait Method Light Trap Method Blood Resource

胶蚧属一新种及其生物学研究初报(同翅目:胶蚧科)

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林业科学 1982年第1期 53-57页

作者：王子清姚德富崔士英梁成杰中国科学院动物研究所中国林业科学研究院

本文记述1969年自云南省寄生在粗糠柴枝条上的胶蚧属(Laccifer)一新种取名为田胶蚧(Laccifer ruralis sp.n.)。此种长期以来因在外部形态上与紫胶蚧(Laccifer LaccaKerr.)相似,所以一直被误认为两者是同一种,或认为是因为寄主不同或分... 详细信息

本文记述1969年自云南省寄生在粗糠柴枝条上的胶蚧属(Laccifer)一新种取名为田胶蚧(Laccifer ruralis sp.n.)。此种长期以来因在外部形态上与紫胶蚧(Laccifer LaccaKerr.)相似,所以一直被误认为两者是同一种,或认为是因为寄主不同或分布不同地区所发生的变异。通过在福建省南靖(同一地区)生长的南岭黄檀(同一寄主)上田胶蚧的生物学观察以及形态方面的比较,我们认为它与紫胶蚧是同域生活的近缘种。现将该新种的形态和生物学介绍如下。新种的正模和副模标本均保存在中同科学院动物研究所。

关键词：同翅目金黄色紫胶虫胶寄主植物雄成虫研究初报生物学