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Advances in Microbiology 2013年第3期3卷 280-288页

作者： Sangrila Sadhu Pallab Kumar Ghosh Tarun Kumar De tushar kanti maiti Microbiology Laboratory Department of Botany the University of Burdwan Burdwan India

A cellulase producing bacterium (E3 strain) was isolated from fecal matter of elephant and identified as Bacillus sp. using 16S rDNA sequenced based molecular phylogenetic approach. While studying the effect of substrates like Carboxymethyl cellulose (CMC), avicel, starch, maltose, sucrose, glucose, fructose, galactose and lactose on cellulase production, it was found that CMC was best carbon source induced cellulase production followed by lactose in this bacterial strain. A positive synergistic effect of lactose with CMC was also observed with enhancement of 5 - 6 times in cellulase production. The optimum cellulase production was recorded with 1% CMC and 1% lactose when added individually in the Omeliansky’s medium. The results showed that addition lactose with CMC greatly enhances the production and activity of various cellulase enzymes. The optimal fermentation conditions for the biosynthesis of cellulase by this strain were found to be temperature: 37℃, pH 7.0. The nitrogen source NH4Cl at 0.15% was optimum for cellulase production by this bacterium.

关键词： Avicelase β-Glucosidase Carboxymethyl Cellulase (CMCase) Lactose

An Approach to the Identification and Characterisation of a Psychrotrophic Lipase Producing Pseudomonassp ADT3 from Arctic Region

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Advances in Bioscience and Biotechnology 2014年第4期5卷 322-332页

作者： Arpita Dey Amarnath Chattopadhyay Pradipta Saha Subhrakanti Mukhopadhyay tushar kanti maiti Sabyasachi Chatterjee Pranab Roy Department of Biotechnology The University of Burdwan Burdwan India Department of Botany The University of Burdwan Burdwan India Department of Microbiology The University of Burd-wan Burdwan India

The aim of this research work was to explore psychrotrophic microbes from soil sample of NyAlesund, Svalbard, arctic region and to investigate their potential use as an effective tool for industrial application. A novel psychrotrophic bacterial strain showed good growth on minimal medium containing lipid as the only carbon source. Microbiological characterisation of the isolate showed that it was a gram negative rod. The strain was tested for the production of extracellular lipase enzyme. The enzymes were partially purified by 90% saturated ammonium sulfate and dialysis for desalting. The bacterium was identified as Pseudomonas sp ADT3 by 16S rRNA amplification and sequencing which had been deposited in the NCBI GenBank with accession number JX914667. Phylogenetic tree was also constructed with MEGA5 software and showed the highest level of sequence similarity with Pseudomonas sp. HC3-13 strain. The microorganism had a growth optimum at pH 8.0 and temperature 22°C. Optimization of different parameters e.g. temperature, pH, incubation time, cofactors etc. was performed for the extracellular lipase activity. The hydrolytic activity of the enzyme was enhanced 5 times by Pb2+ but strongly inhibited by heavy metals Hg2+ as well as EDTA and β-mercaptoethanol. For the molecular weight estimation of enzyme SDS-PAGE was done which showed an inducible band of approximately 13.9 KDa. Activity staining and mass spectrometry techniques were also performed.

关键词： Psychrotrophic Arctic Extracellular Lipase 16S rRNA Mass Spectrometry

A Depth Wise Diversity of Free Living N2Fixing and Nitrifying Bacteria and Its Seasonal Variation with Nitrogen Containing Nutrients in the Mangrove Sediments of Sundarban, WB, India

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Open Journal of Marine Science 2013年第2期3卷 112-119页

作者： Subhajit Das Dipnarayan Ganguly tushar kanti maiti Abhishek Mukherjee Tapan Kumar Jana Tarun Kuma De Department of Marine Science Calcutta University Kolkata India Microbiology Laboratory Department of Botany Burdwan University Burdwan India

Mangrove provides a unique ecological niche to different microbes which play various roles in nutrient recycling as well as various environmental activities. The highly productive and diverse microbial community living in mangrove ecosystems continuously transforms dead vegetation and recycle nitrogen, phosphorus, sulphur and other nutrients that can later be used by the plants. Mangrove ecosystems are rich in organic matter, and however, in general, they are nutrient-deficient ecosystems, especially of nitrogen and phosphorus. The present study investigated depth wise variation of Nitrifying bacteria, Nitrogen fixing bacteria, total bacterial population along with nitrate-nitrogen, nitrite-nitrogen and other physicochemical parameters of soil during pre-monsoon, monsoon and post-monsoon periods at three different sampling stations of mangrove sediments viz. deep forest region, rooted region and unrooted region. The microbial population was also found maximum in the deep forest sediment relative to the other two sites. Populations of cultureable microbes were found maximum in surface soil and decreased with increase in depth in Sundarban mangrove environment. A decreasing trend of total microbial load, nitrifying and nitrogen fixing bacteria with increase in depth were recorded throughout the year. Present study revealed the relationship among depth integrated variations of physicochemical components (viz. soil temperature, pH, salinity, nitrite nitrogen and nitrate nitrogen concentration) and total microbial load, nitrifying and nitrogen fixing bacteria microbial populations.

关键词： Nitrifying Bacteria N2 Fixing Bacteria Bacterial Population Mangrove Sediment

Depth Integrated Microbial Community and Physico-Chemical Properties in Mangrove Soil of Sundarban, India

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Advances in Microbiology 2012年第3期2卷 234-240页

作者： Subhajit Das Minati De Dipnarayan Ganguly tushar kanti maiti Abhishek Mukherjee Tapan Kumar Jana Tarun Kuma De Department of Marine Science Calcutta University Kolkata India Maniktala Siksha Bhavan Kolkata India Microbiology Laboratory Department of Botany Burdwan University Burdwan India

In the Sundarban Mangrove forest microbial activities are dominantly involved in both the mineralization and decomposition processes that regulate nutrient profile in soil of different depth. It was found that besides changing the water and soil quality, monsoonal cycle plays a crucial role in regulating microbial population distribution in the mangrove soil. Statistical analyses revealed that organic carbon was the most significant factor that regulated the total microbial population. The cellulose degrading bacteria, [mean value of CFU 14.32 × 106 (gm dry weight of soil)–1] was dominant throughout the year. The sulfate reducing bacteria showed an increasing trend along depth with a minimum value at the surface i.e. 6.113 × 106 (gm dry weight of soil)–1 and 12.312 × 106 (gm dry weight of soil)–1 at a depth of 60 cm. Intensification of monsoonal cycle could heavily affect microbe dominated soil biogeochemistry and subsequent change in the regional ecology of the Sundarban Mangrove Forest.

关键词： Sundarban Mangrove Microbial Population Monsoonal Cycle Nutrient Concentration

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细菌产生的纤维素酶(综述)(续1)

国外畜牧学（猪与禽） 2018年第11期38卷 57-61页

作者：严鸿林唐彩琰 Sangrila Sadhul tushar kanti maiti

上期回顾：上期主要介绍了纤维素酶在生产中的重要作用,重点阐述纤维素酶的分类及如何筛选产生纤维素酶的细菌.3使用深层液态发酵、固态发酵或者培养生产纤维素酶发酵是一种通过多种微生物将复杂的底物转化为简单化合物的生物转化技术.... 详细信息

上期回顾：上期主要介绍了纤维素酶在生产中的重要作用,重点阐述纤维素酶的分类及如何筛选产生纤维素酶的细菌.3使用深层液态发酵、固态发酵或者培养生产纤维素酶发酵是一种通过多种微生物将复杂的底物转化为简单化合物的生物转化技术.它被广泛地用于生产纤维素酶,并已在工业中广泛应用.多年来,由于其经济和环境优势,发酵技术已经变得非常重要.

关键词：纤维素酶细菌生物转化技术综述液态发酵固态发酵环境优势发酵技术

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细菌产生的纤维素酶(综述)(续2)

国外畜牧学（猪与禽） 2018年第12期38卷 18-20页

作者：严鸿林唐彩琰 Sangrila Sadhu1 tushar kanti maiti 不详

本期主要介绍纤维素酶在细菌体系中的作用方式、细菌共培养以及纤维素酶基因在异源宿主中的克隆与表达。

关键词：纤维素酶共培养克隆细菌

Optimizing calcination of coal by-products for maximizing REE leaching recovery and minimizing Al,Ca,and Fe contamination

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Journal of Rare Earths 2024年第7期42卷 1354-1365,I0006页

作者： tushar Gupta Ahmad Nawab Rick Honaker MP Materials Mountain Pass Mine 67750 Bailey RoadMountain PassCA92366USA Department of Mining Engineering University of KentuckyLexingtonKY40506USA

High-temperature calcination is effective in improving the leaching characteristics of rare earth elements(REEs)from conventional REE-bearing minerals by transforming them into more leachable ***,the recovery of contaminants such as Al,Fe,and Ca also increases *** objective of the study was to maximize the REE extraction while minimizing the concentration of contaminant ions(Al,Ca,and Fe)in the leach *** density fractions of the coarse refuse from the Baker and Fire Clay seam coal refuse were pulverized to a top size of 180μm and used as a feedstock for the *** the samples generated from thermal treatment were leached at 1%(w/v)using 1.2 mol/L sulfuric acid at 75℃ for 2 *** results from a parametric program based on a Box-Behnken design were used for modeling and optimization of three operating parameters associated with the lab-scale calcination *** parameters included temperature(400-800℃),ramp rate(2-10℃/min)and holding time(0-120 min).It was determined that the calcination temperature was the most significant parameter influencing REE *** majority of the heavy rare earth elements(HREEs)were found to be present in an insoluble form in the calcination products and were marginally affected by thermal treatment.A strong correlation between light rare earth elements recovery(LREEs)and Al suggested a possible association with *** optimum operating conditions for high REE recovery with comparatively lower contaminant concentration for 1.6 specific gravity float and 2.2 sink fractions of Baker and Fire Clay seam material were determined to be at 776,800℃ and 407,800℃,calcining temperatures,respectively.A higher optimum temperature of 1.6 float fraction of Baker seam relative to Fire Clay seam material was due to elevated pyrite concentration in Baker seam *** treatment at 400℃ converted pyrite into a soluble intermedium iron oxide,resulting in a high Fe content in the ***

关键词： Calcination Optimization Rare earth elements Coal refuse

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细菌产生的纤维素酶(综述)

国外畜牧学（猪与禽） 2018年第9期38卷 51-54页

作者：严鸿林(译) 张配配(校) 唐彩琰(制表) sangrila sadhu1 tushar kanti maiti 不详

纤维素是地球上一种丰富的天然生物聚合物和主要的农业废弃物。这种纤维素生物质是一种具有被生物转化为增值生物产品巨大潜力的丰富的可再生资源。它可以被纤维素分解菌产生的纤维素酶降解。这种酶有多种工业用途,目前被认为是最重要... 详细信息

纤维素是地球上一种丰富的天然生物聚合物和主要的农业废弃物。这种纤维素生物质是一种具有被生物转化为增值生物产品巨大潜力的丰富的可再生资源。它可以被纤维素分解菌产生的纤维素酶降解。这种酶有多种工业用途,目前被认为是最重要的工业酶。本综述将探讨纤维素酶的应用、分类和定量以及纤维素分解菌的类型和筛选,也将介绍通过液态发酵和固态发酵方式生产纤维素酶、纤维素酶的性质以及纤维素酶基因的克隆和表达的最新知识。此外,本文还将讨论研究纤维素酶的生物技术及其未来展望。

关键词：纤维素分解菌生物转化纤维素酶内切葡聚糖酶外切葡聚糖酶 β-葡萄糖苷酶纤维小体

A critical review on solid-state welding of high entropy alloys-processing,microstructural characteristics and mechanical properties of joints

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Defence Technology（防务技术） 2024年第4期34卷 78-133页

作者： tushar Sonar Mikhail Ivanov Evgeny Trofimov Aleksandr Tingaev Ilsiya Suleymanova Department of Welding Engineering Institution of Engineering and TechnologySouth Ural State University(National Research University)Chelyabinsk454080Russia Department of Materials Science Physical and Chemical Properties of MaterialsInstitution of Engineering and TechnologySouth Ural State University(National Research University)Chelyabinsk454080Russia

The high entropy alloys(HEAs)are the newly developed high-performance materials that have gained significant importance in defence,nuclear and aerospace sector due to their superior mechanical properties,heat resistance,high temperature strength and corrosion *** alloys are manufactured by the equal mixing or larger proportions of five or more alloying *** exhibit superior mechanical performance compared to traditional engineering alloys because of the extensive alloying composition and higher entropy of *** state welding(SSW)techniques such as friction stir welding(FSW),rotary friction welding(RFW),diffusion bonding(DB)and explosive welding(EW)have been efficiently deployed for improving the microstructural integrity and mechanical properties of welded HEA *** HEA interlayers revealed greater potential in supressing the formation of deleterious intermetallic phases and maximizing the mechanical properties of HEAs *** similar and dissimilar joining of HEAs has been manifested to be viable for HEA systems which further expands their industrial ***,the main objective of this review paper is to present a critical review of current state of research,challenges and opportunities and main directions in SSW of HEAs mainly CoCrFeNiMn and Al_xCoCrFeNi *** state of the art of problems,progress and future outlook in SSW of HEAs are critically reviewed by considering the formation of phases,microstructural evolution and mechanical properties of HEAs joints.

关键词： High entropy alloys Solid state welding Microstructure Mechanical properties

LiDAR-based estimation of bounding box coordinates using Gaussian process regression and particle swarm optimization

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Biomimetic Intelligence & Robotics 2024年第1期4卷 24-35页

作者： Vinodha K. E.S.Gopi tushar Agnibhoj Department of Electronics and Communication Engineering National Institute of Technology TiruchirappalliTamil Nadu620015India

Camera-based object tracking systems in a given closed environment lack privacy and *** this study,light detection and ranging(LiDAR)was applied to track objects similar to the camera tracking in a closed environment,guaranteeing privacy and *** primary objective was to demonstrate the efficacy of the proposed technique through carefully designed experiments conducted using two *** Scenario I,the study illustrates the capability of the proposed technique to detect the locations of multiple objects positioned on a flat surface,achieved by analyzing LiDAR data collected from several locations within the closed *** II demonstrates the effectiveness of the proposed technique in detecting multiple objects using LiDAR data obtained from a single,fixed ***-time experiments are conducted with human subjects navigating predefined *** individuals move within an environment,while LiDAR,fixed at the center,dynamically tracks and identifies their locations at multiple *** demonstrate that a single,strategically positioned LiDAR can adeptly detect objects in motion around ***,this study provides a comparison of various regression techniques for predicting bounding box *** process regression(GPR),combined with particle swarm optimization(PSO)for prediction,achieves the lowest prediction mean square error of all the regression techniques examined at *** tuning of GPR using PSO significantly minimizes the regression *** of the experiment pave the way for its extension to various real-time applications such as crowd management in malls,surveillance systems,and various Internet of Things scenarios.

关键词： LiDAR Data acquisition Bounding box Gaussian process regression Particle swarm optimization(PSO)