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Real-time in situ three-dimensional integral videography and surgical navigation using augmented reality: a pilot study

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International Journal of Oral Science 2013年第2期5卷 98-102页

作者： Hideyuki Suenaga Huy Hoang Tran Hongen Liao Ken Masamune Takeyoshi Dohi Kazuto Hoshi Yoshiyuki Mori tsuyoshi takato Department of Oral-Maxillofacial Surgery Dentistry and OrthodonticsThe University of Tokyo Hospital Department of Mechano-Informatics Graduate School of Information Science and TechnologyThe University of Tokyo Department of Bioengineering Graduate School of EngineeringThe University of Tokyo

To evaluate the feasibility and accuracy of a three-dimensional augmented reality system incorporating integral videography for imaging oral and maxillofacial regions, based on preoperative computed tomography data. Three-dimensional surface models of the jawbones, based on the computed tomography data, were used to create the integral videography images of a subject＇s maxillofacial area. The three-dimensional augmented reality system （integral videography display, computed tomography, a position tracker and a computer） was used to generate a three-dimensional overlay that was projected on the surgical site via a half-silvered mirror. Thereafter, a feasibility study was performed on a volunteer. The accuracy of this system was verified on a solid model while simulating bone resection. Positional registration was attained by identifying and tracking the patient/surgical instrument＇s position. Thus, integral videography images of jawbones, teeth and the surgical tool were superimposed in the correct position. Stereoscopic images viewed from various angles were accurately displayed. Change in the viewing angle did not negatively affect the surgeon＇s ability to simultaneously observe the three-dimensional images and the patient, without special glasses. The difference in three-dimensional position of each measuring point on the solid model and augmented reality navigation was almost negligible （〈1 mm）; this indicates that the system was highly accurate. This augmented reality system was highly accurate and effective for surgical navigation and for overlaying a three-dimensional computed tomography image on a patient＇s surgical area, enabling the surgeon to understand the positional relationship between the preoperative image and the actual surgical site, with the naked eye.

关键词： augmented reality computed tomography integral videography three-dimensional image

Evaluation of <i>in vivo</i>migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

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Open Journal of Regenerative Medicine 2013年第4期2卷 93-98页

作者： Mariko Matsuyama Yuko Fujihara Ryoko Inaki Satoru Nishizawa Satoru Nagata tsuyoshi takato Kazuto Hoshi Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Sensory and Motor System Medicine The University of Tokyo Hospital Tokyo Japan NAGATA Microtia and Reconstructive Plastic Surgery Clinic Toda Saitama Japan Translational Research Center The University of Tokyo Hospital Tokyo Japan

For regenerative medicine, clarification of in vivo migration of transplanted cells is an important task to secure the safety of transplanted tissue. We had prepared tissue-engineered cartilage consisting of cultured chondrocytes with collagen hydrogel and a biodegradable porous polymer, and we clinically applied it for treatment of craniofacial anomaly. To verify the safety of this tissue-engineered cartilage, we had syngenically transplanted the tissue-engineered cartilage using chondrocytes harvested from EGFP-transgenic mice into subcutaneous pocket of wild type mice, and investigated localizations of transplanted chondrocytes in various organs including cerebrum, lung, liver, spleen, kidney, auricle, gastrocnemius, and femur. After 8 to 24 weeks of the transplantation, accumulation of cartilaginous matrices was observed in tissue-engineered cartilage, while EGFP-positive transplanted chondrocytes were localized in this area. Otherwise, no EGFP was immunohistochemically detected in each organ, suggesting that subcutaneously-transplanted chondrocytes do not migrate to other organs through the circulation. In cartilage tissue engineering using cultured chondrocytes, risk for migration and circulation of transplanted cells seemed negligible, and that ectopic growth of the cells was unlikely to occur, showing that this is safe technique with regard to the in vivo migration of transplanted cells.

关键词： Cartilage Tissue Engineering Chondrocyte Migration

A Case of Pleomorphic Adenoma of the Parotid Gland with Multiple Local Recurrences through Facial to Cervical Regions

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Open Journal of Stomatology 2014年第9期4卷 441-445页

作者： Masanobu Abe Yoshiyuki Mori Yuki Kanno Kazuto Hoshi Hideto Saijo Takahiro Abe Kazumi Ohkubo tsuyoshi takato Department of Oral & Maxillofacial Surgery University of Tokyo Hospital Tokyo Japan

Pleomorphic adenoma is the most common benign parotid gland tumor. Although its local recurrence rate is known to be high, the recurrence extending to the cervical region is rare. Here we report a case of a young female (25 years old) with pleomorphic adenoma of the parotid gland which showed multiple recurrences through facial to cervical regions over a span of eight years. We also discuss how this benign tumor with a high recurrence rate has been treated in other cases, and how it should be treated.

关键词： Pleomorphic Adenoma Parotid Gland Salivary Neoplasm

Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage

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Materials Sciences and Applications 2013年第8期4卷 73-78页

作者： Yoshiyuki Mori Sanshiro Kanazawa Makoto Watanabe Hideyuki Suenaga Kazumi Okubo Satoru Nagata Yuko Fujihara tsuyoshi takato Kazuto Hoshi Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Division of Tissue Engineering The University of Tokyo Hospital Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Division of Tissue Engineering The University of Tokyo Hospital Tokyo Japan NAGATA Microtia and Reconstructive Plastic Surgery Clinic Saitama Japan

The efficiency of substance exchange may be decreased when the thickness and volume of such a tissue-engineered cartilage that is composed of cultured cells and porous scaffold increase. Moreover, during the transport of this construct with complicated shapes, excessive and focal mechanical loading may cause deformation. The establishment of incubation and transport methods is necessary for the three-dimensional tissue-engineered cartilage. Therefore, we investigated the preparation of an agarose mold with a concavity similar to the shape of 3-dimensional tissue-engineered cartilage to prevent excessive and focal concentration of stress, while avoiding interference with substance exchange as much as possible. Firstly, we investigated the preparation at 1% - 4% agarose concentrations. Since the mechanical strength was insufficient at 1%, 2% was regarded as appropriate. Using 2% agarose, we prepared a mold with a 5 × 5 × 5 mm concavity to accommodate tissue-engineered cartilage (5 × 5 × 5 mm mixture of 1.5 × 107 cells and collagen gel), and stored the regenerative cartilage in it for 2 and 24 hours. On comparison with storage in a plastic mold with the same shape in which substance exchanged from side and bottom was impossible, although no significant differences were noted in the number or viability of cells after 2 hours, these were markedly reduced in the plastic mold after 24 hours. It was confirmed that favorable cell numbers and viability were maintained by immediately retaining the regenerative cartilage in the culture medium in the agarose mold and keeping the temperature at 37°C. Since this agarose mold also buffers against mechanical forces loaded on the three-dimensional regenerative tissue, it may be useful as a container for storage and transport of large-sized three-dimensional regenerative tissue.

关键词： Tissue Engineering Cartilage Container Storage Transport

Hollow Fiber Module Applied for Effective Proliferation and Harvest of Cultured Chondrocytes

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Materials Sciences and Applications 2013年第8期4卷 62-67页

作者： Yoshiyuki Mori Makoto Watanabe Sakae Nakagawa Yukiyo Asawa Satoru Nishizawa Kazumi Okubo Hideto Saijo Satoru Nagata Yuko Fujihara tsuyoshi takato Kazuto Hoshi Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Division of Tissue Engineering The University of Tokyo Hospital Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Cartilage & Bone Regeneration (Fujisoft) Graduate School of Medicine The University of Tokyo Tokyo Japan Division of Tissue Engineering The University of Tokyo Hospital Tokyo Japan Department of Sensory and Motor System Medicine Graduate School of Medicine The University of Tokyo Tokyo Japan Division of Tissue Engineering The University of Tokyo Hospital Tokyo Japan NAGATA Microtia and Reconstructive Plastic Surgery Clinic Saitama Japan Nomura Unison Co. Ltd Nagano Japan

Steady and useful culture for chondrocytes is essential for cartilage regenerative medicine. However, in conventional plate culture, the chondrocytes become dedifferentiated and lose their ability to make cartilage matrices. Three-dimensional culture mimicking the physiological environment in native chondrocytes is useful to maintain the chondrocyte properties during the proliferation culture. However, the three-dimensional culture is practically a hard task due to difficult harvest of the cells. Thus, we attempted to apply porous materials, hollow fibers for the three-dimensional culture, and developed their module to realize the effective harvest of the cells. Polyethersulfone-based hollow fibers, whose safety and cell affinity were confirmed by the experiment of the coculture with human chondrocytes, were collected to fabricate a module. The hollow fiber module was installed with screw ends, and enabled the easy removal of chondrocytes from the inner unit. Cultured human chondrocytes embedded within collagen hydrogel were put into the outer lumen of the hollow fiber module, while chondrocyte prolfieration medium was perfused through the inner lumen at 0 to 30 mL/min. After 2 weeks’ culture, the flow rate of 3 to 10 mL/min effectively supported the chondrocyte proliferation. Then, long-term culture using the hollow fiber module at flow rate of 5 mL/min was performed, revealing that the cell growth in this module at 3 weeks was approximately twice larger than that in static culture. The numbers of viable cells could be maintained by week 7. The hollow fiber module installed with screw ends can effectively culture and harvest the chondrocytes.

关键词： Hollow Fiber Module Proliferation Cell Culture Chondrocyte Tissue Engineering

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应用Furlow法治疗腭裂的经验

中国修复重建外科杂志 1991年第2期 110-110页

作者： tsuyoshi takato,杨桂兰日本静冈市儿童医院腭裂科内蒙大雁矿务局医院

为了改善腭裂修补后语言表达能力,Furlow1986年报道了一种腭裂成形术的新方法。术后上颌骨仍能充分发育。设计两个相对的“Z”形皮瓣,一个取自口腔,另一个取自鼻层,用来闭合软腭。这些皮瓣移位后,构成一个腭肌悬带横跨腭裂,顺“Z”形整... 详细信息

为了改善腭裂修补后语言表达能力,Furlow1986年报道了一种腭裂成形术的新方法。术后上颌骨仍能充分发育。设计两个相对的“Z”形皮瓣,一个取自口腔,另一个取自鼻层,用来闭合软腭。这些皮瓣移位后,构成一个腭肌悬带横跨腭裂,顺“Z”形整形的中央部分所获得的长度得以增长软腭,不需要利用来自硬腭的组织。

关键词： Furlow