检索结果-南通市图书馆

A novel aptamer-based small RNA delivery platform and its application to cancer therapy

Genes & Diseases 2023年第3期10卷 1075-1089页

作者： toshihiko tanno Peng Zhang Christopher Bailey Yin Wang Wannaporn Ittiprasert Martin Devenport Pan Zheng Yang Liu OncoC4 Inc.RockvilleMD 20850USA Division of Immunotherapy Institute of Human VirologyUniversity of Maryland School of MedicineBaltimoreMD 21201USA Department of Microbiology Immunology and Tropical MedicineSchool of Medicine and HygieneGeorge Washington UniversityNW WashingtonDC 20037USA

Major challenges such as nuclease degradation, rapid renal clearance, non-specific delivery, poor cellular uptake and inflammatory response have limited the clinical application of small RNA-mediated gene silencing. To overcome these challenges, we designed a novel targeting small RNA delivery platform comprising of three oligonucleotides: (1) a guide RNA sequence, (2) part of a passenger sequence linked to a DNA aptamer via a PEG linker, and (3) another passenger sequence conjugated to cholesterol, which assemble through complementary base pair annealing. Remarkably, in the presence of magnesium, this molecule self-assembled into a nanoparticle with a hydrophobic cholesterol core, hydrophilic RNA oligonucleotide shell and PEG-linked DNA aptamer flare. The nanoparticles conferred protection to the RNA oligonucleotides against nuclease degradation, which increased bioavailability, and reduced systemic inflammatory responses. The aptamer allowed targeted delivery of RNA therapeutics through cell-specific surface markers, and once inside the cell, the nanoparticles induced lysosomal leakage that released the RNA oligonucleotides into the cytosol to achieve gene silencing. We created a c-Kit-targeting miR-26a delivery particle that specifically accumulated in c-Kit^(+) breast cancer, significantly increased T cell recruitment, and inhibited tumor growth. Regression of large established tumors were achieved when the nanoparticle was used in combination with anti-CTLA-4 monoclonal antibody.

关键词： Aptamer Cancer Micellar nanoparticle miRNA Tar get delivery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Differences in clinicopathological characteristics between lipohypertrophy and localized insulin-derived amyloidosis:A scoping review

引用

Chronic Diseases and Translational Medicine 2024年第1期10卷 22-30页

作者： Kanae Mukai Hiromasa tanno Junko Sugama toshihiko Yanagita Emi Kanno Department of Clinical Nursing Faculty ofHealth SciencesInstitute of MedicalPharmaceutical and Health SciencesKanazawa UniversityKanazawaJapan Department of Translational Science forNursing Tohoku University Graduate School ofMedicineSendaiJapan Research Center for Implementation NursingScience Initiative School of Health SciencesFujita Health UniversityToyoakeJapan Department of Clinical Pharmacology Schoolof NursingUniversity of MiyazakiMiyazakiJapan

Insulin is used as a therapeutic agent in patients with diabetes,and cutaneous lipohypertrophy(LH)and localized insulin-derived amyloidosis(LIDA)are well-known adverse effects associated with insulin *** clinical implications,management,assessment methods,and pathological differentiation of LH and LIDA have been recently *** review was to update our knowledge of the pathological differentiation,effects of insulin absorption,hypoglycemic events,and recent assessment methods for LH and LIDA.A scoping review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta Analyses extension for Scoping Reviews *** studies and case reports in English were also *** and Scopus databases were searched for keywords to identify papers published up to January 2022.A total of 113 studies were identified through a database search,and 31 were eligible for inclusion in this scoping *** the 31 studies included in this review,patients with type 2 diabetes had high frequencies of LH and *** outcome parameters were assessed using pathological findings and *** is mainly determined by pathological methods,such as hematoxylin and eosin and Congo red *** in vitro and in vivo LIDA models of LIDA have been *** results suggest that pathological analysis is required to identify LH and *** is important to consider LIDA,as it likely influences insulin adsorption and glycemic *** several studies have evaluated the LIDA process,little is known about the mechanisms underlying the development of adverse effects associated with insulin injections.

关键词： diabetes mellitus insulin injection lipohypertrophy localized insulin-derived amyloidosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：