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An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood

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microsystems & Nanoengineering 2024年第3期10卷 293-303页

作者： Zhehan ma Jianping Xia Neil Upreti Emeraghi David Joseph Rufo Yuyang Gu Kaichun Yang Shujie Yang Xiangchen Xu Jean Kwun Eileen Chambers tony Jun Huang Thomas Lord Department of mechanical Engineering and materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Pediatrics Duke UniversityDurhamNCUSA Duke Transplant Center Department of SurgeryDuke University School of MedicineDurhamNCUSA

Separating plasma from whole blood is an important sample processing technique required for fundamental biomedical research,medical diagnostics,and therapeutic *** protocols for plasma isolation require multiple centrifugation steps or multiunit microfluidic processing to sequentially remove large red blood cells(RBCs)and white blood cells(WBCs),followed by the removal of small ***,we present an acoustofluidic platform capable of efficiently removing RBCs,WBCs,and platelets from whole blood in a single *** leveraging differences in the acoustic impedances of fluids,our device generates significantly greater forces on suspended particles than conventional microfluidic approaches,enabling the removal of both large blood cells and smaller platelets in a single *** a result,undiluted human whole blood can be processed by our device to remove both blood cells and platelets(>90%)at low voltages(25 Vpp).The ability to successfully remove blood cells and platelets from plasma without altering the properties of the proteins and antibodies present creates numerous potential applications for our platform in biomedical research,as well as plasma-based diagnostics and ***,the microfluidic nature of our device offers advantages such as portability,cost efficiency,and the ability to process small-volume samples.

关键词： remove separation removing

Epidemiological, Clinical and Evolutionary Aspects of Human African Trypanosomiasis in Children in Nola

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Open Journal of Pediatrics 2024年第2期14卷 344-358页

作者： Brice Olivier Bogning mejiozem Gaspard Tékpa Synthia Ningatoloum Nazita Jocelyn tony Nengom Danebera Lydie Verlaine Carine Judith Kiteze Nguinzanémou Rostand Juste Koyangboi Kombaya Iris Vanessa Gaspiet Sonny Jean Chrysostome Gody Complexe Hospitalier Pédiatrique de Bangui Bangui République Centrafricaine Service des maladies Infectieuses H&#244pital de l’Amitié Bangui République Centrafricaine Faculté des Sciences de la Santé de l’Université de Bangui Bangui République Centrafricaine Centre Hospitalo Universitaire Communautaire de Bangui Bangui République Centrafricaine Faculté de médecineet des Sciences Biomédicales Université de Yaoundé 1 Yaoundé Cameroun

Background: Human African trypanosomiasis (HAT) occurs in three historical foci in the Central African Republic. Objective: To describe the morbi-mortality of childhood HAT in rural Central African Republic with the aim of early management. methods: Descriptive and analytic cross-sectional study conducted from January 1, 2017 to march 30, 2018 at Nola prefectural hospital. This study was included all children seen as outpatients and/or hospitalized in the pediatric wards, in whom the diagnosis of HAT was confirmed. Statistical analysis was performed using Epi-info software. Results: Forty children were included from Bilolo (60%), Nola (30%) and Salo (10%). The sex ratio was 0.66 with a median age of 8.65 ± 12.48 years. Fever (82.5%), nocturnal insomnia (75%), daytime somnolence (67.5%), headache (65%), polyarthralgia (62.5%), convulsions 52.5% (n = 21), tremor (27.5%), trypanidism (7.5%) and delirium (2.5%) were the main functional signs. Examination signs were adenopathy (52.5%), paresthesia (30%), decreased cutaneous-abdominal reflexes (25%) and osteoarticular reflexes (17.5%), hyperesthesia (20%), extrapyramidal hypertonia (15%) and depression (2.5%). The children were in the lymphatic-blood phase in 65% of cases, and meningoencephalitis in 35%. Pentamidine was administered in 65% of cases. Four children died and 6 had neurological sequelae. There was an association between age under 5, Bilolo’s focus, the children’s history and the severity of the disease. Age under 5, gender, household, children’s activity, history and occurrence of sequelae were also associated. Conclusion: HAT remains a permanent threat to Central African children. Any clinical presentation combining long-term infectious signs and unexplained neurological or neuropsychological disorders must be treated with caution.

关键词： morbidity-mortality Child HAT Nola CAR

Fungal diversity notes 111-252-taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2015年第6期75卷 27-274页

作者： Hiran A.Ariyawansa Kevin D.Hyde Subashini C.Jayasiri Bart Buyck K.W.Thilini Chethana Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Ruvishika S.Jayawardena Robert Lücking masoomeh Ghobad-Nejhad Tuula Niskanen Kasun m.Thambugala Kerstin Voigt Rui Lin Zhao Guo-Jie Li mingkwan Doilom Saranyaphat Boonmee Zhu L.Yang Qing Cai Yang-Yang Cui Ali H.Bahkali Jie Chen Bao Kai Cui Jia Jia Chen monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang Hongsanan E.B.Gareth Jones Ellen Larsson Wen Jing Li Qi-Rui Li Jian Kui Liu Zong Long Luo Sajeewa S.N.maharachchikumbura Ausana mapook Eric H.C.mcKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Singtripop Kazuaki Tanaka Cheng ming Tian Saowaluck Tibpromma mohamed A.Abdel-Wahab Dhanushka N.Wanasinghe Nalin N.Wijayawardene Jin-Feng Zhang Huang Zhang Faten A.Abdel-Aziz mats Wedin martin Westberg Joseph F.Ammirati Timur S.Bulgakov Diogo X.Lima tony m.callaghan Philipp Callac Cheng-Hao Chang Luis F.Coca manuela Dal-Forno Veronika Dollhofer Kateřina Fliegerová Katrin Greiner Gareth W.Griffith Hsiao-man Ho Valerie Hofstetter Rajesh Jeewon Ji Chuan Kang Ting-Chi Wen Paul m.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing Hong Li Zou Yi Liu Xing Zhong Liu Kare Liimatainen H.Thorsten Lumbsch misato matsumura Bibiana moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.m.de Azevedo Santiago Sujinda Sommai Yu Song Carlos A.F.de Souza Cristina m.de Souza-motta Hong Yan Su Satinee Suetrong Yong Wang Syuan-Fong Wei Ting Chi Wen Hai Sheng Yuan Li Wei Zhou martina Réblová Jacques Fournier Erio Camporesi J.Jennifer Luangsa-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana m.millanes Ralph S.Common marc Stadler Ji Ye Yan XingHong Li Hye Won Lee Thi T.T.Nguyen Hyang Burm Lee Eliseo Battistin Orlando marsico Alfredo Vizzini Jordi Vila Enrico Ercole Ursula Eberhardt Giampaolo Simonini Hua-An Wen Xin-Hua Chen Otto mie Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina Centre of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailan Département Systématique et Evolution CP 39ISYEBUMR 7205 CNRS MNHN UPMC EPHEMuséum National d’Histoire Naturelle12 Rue BuffonF-75005 ParisFrance Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shu Guang Hua Yuan Zhong LuHaidian DistrictBeijing 10097China microbial Drugs Helmholtz-Centre for Infection Research Inhoffenstrasse 738124 BraunschweigGermany Staatliches museum für Naturkunde Stuttgart Rosenstein 1D-70191StuttgartGermany Institute of microbiology Beijing Forestry UniversityPO Box 61Beijing 100083China State Key Laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101China The Herbarium(ANDA) Universitas AndalasPadangSumatera Barat 25163Indonesia Botanischer Garten&Botanisches museum Berlin Freie Universität BerlinKönigin-Luise-Straße 6-814195 BerlinGermany Department of Biotechnology Iranian Research Organization for Science and Technology(IROST)P.O.Box 3353-5111Tehran ***Iran Jodrell Laboratory Royal Botanic GardensKew TW93DSUK Leibniz Institute for Natural Product Research and Infection Biology Jena Microbial Resource CollectionAdolf-Reichwein-Strasse 2307745 JenaGermany Department of Botany and microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia The United Graduate School of Agricultural Sciences Iwate University18-8 Ueda 3 ChomeMorioka 020-8550Japan Faculty of Agriculture and Life Sciences Hirosaki University3 Bunkyo-choHirosakiAomori 036-8561Japan Department of Biological and Environmental Sciences University of GothenburgBox 461SE

This paper is a compilation of notes on 142 fungal taxa,including five new families,20 new genera,and 100 new species,representing a wide taxonomic and geographic *** new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced based on their distinct lineages and unique *** new Dothideomycete genera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),Brunneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),Galliicola,Populocrescentia and Vagicola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elongatopedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(macrodiplodiopsidaceae)are *** newly described species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria bromicola(Amniculicolaceae),Flammeascoma lignicola(Anteagloniaceae),Ascocylindrica marina(Ascocylindricaceae),Lembosia xyliae(Asterinaceae),Diplodia crataegicola and Diplodia galiicola(Botryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),Brunneoclavispora bambusae,Neolophiostoma pigmentatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fagi(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasubmersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(macrodiplodiopsidaceae),microthyrium buxicola and Tumidispora shoreae(microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria subcylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,Galiicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and Vagicola vagans(Phaeosphaeriaceae),Elon

关键词： Fungi Taxonomy New genus New species Phylogeny

Developing a two-decadal time-record of rice field maps using Landsat-derived multi-index image collections with a random forest classifier:A Google Earth Engine based approach

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Information Processing in Agriculture 2024年第2期11卷 260-275页

作者： W.Ashane m.Fernando I.P.Senanayake Department of Nano Science Technology Wayamba University of Sri LankaKuliyapitiya 60200Sri Lanka Dchoolf Engineing CllegeoEngineeringSience and EnionmentThe UniversyewcastleCallaghanW2Austraia

Historic maps showing the temporal distribution of rice fields are important for precision agriculture,irrigation optimisation,forecasting crop yields,land use management and formulating ***,mapping rice felds using traditional ground surveys is impractical when high cost,time and labour requirements are considered,and the availability of such detailed records is *** satellite remote sensing appears to be a viable solution,conventional segmentation and classification methods with spectral bands are often unable to contrast the distinct characteristics between rice fields and other vegetation *** this end,we explored a novel,Google Earth Engine(GEE)based multiindex random forest(RF)classification approach to map rice fields over two *** images from 2000 to 2020 of two Sri Lankan rice cultivation districts were extracted from GEE and a multi-index RF classification algorithm was applied to distinguish the rice *** results showed above 80%accuracy for both training and validation,when compared against high spatial resolution Google Earth *** essence,multi-index sampling and RF together synergised the compelling classifcation accuracy by effectively capturing vegetation,water(ponding)and soil characteristics unique to the rice felds using a single-click *** maps developed in this study were further compared against the mODIS land cover type product(mCD12Q1)and the corresponding superior statistics on rice fields demonstrated the robustness of the proposed *** work seeking effective index combinations is recommended,and this approach can potentially be extended to other crop analyses elsewhere.

关键词： Google Earth Engine(GEE) Image classification Random forest mapping rice fields Time series analysis Vegetation index

modelling the development of biological structures displaying longitudinal geometries in vitro:culturing pluripotent stem cells on plasma-treated,growth factor-coupled polycaprolactone fibres

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Engineered Regeneration 2024年第1期5卷 124-138页

作者： Badwi B.Boumelhem Stuart T.Fraser Syamak Farajikhah Rachel A.Shparberg michael B.morris marcela m.m.Bilek Anyu Zhang Behnam Akhavan Simon Fleming maryanne Large School of Physics Faculty of ScienceUniversity of SydneyAustralia Sydney Nano Institute University of SydneyAustralia School of Chemical and Biomolecular Engineering University of SydneyAustralia School of medical Sciences Faculty of Medicine and HealthUniversity of SydneyAustralia School of Biomedical Engineering Faculty of EngineeringUniversity of SydneyAustralia School of Engineering University of NewcastleCallaghanNSW 2308Australia Hunter medical Research Institute(HmRI) Precision Medicine ProgramNew Lambton Heights NSW 2305Australia

many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear *** these three-dimensional structures in vitro is challenging:the best-defined stem-cell differentiation systems are mono-layer cultures or organoids using pluripotent stem *** stem cells can differentiate into functionally mature cells depending on the signals received,holding great promise for regenerative ***,the integration of in vitro differentiated cell types into diseased tissue remains a *** scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the ***,we have taken a biomimicry approach to generate longitudinal structures in *** this approach,mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone(PCL)fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently *** demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells:neurons,vascular endothelial cells,osteoclasts,adipocytes,and cells of the erythroid,myeloid,and lymphoid *** arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue.

关键词： Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment Polycaprolactone

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey （macaca mulatta）

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Asian Journal of Andrology 2015年第6期17卷 972-980,I0009页

作者： mahmoud Huleihel Seyedmehdi Nourashrafeddin tony m Plant The Shraga Segal Department of microbiology Immunology and Genetics Faculty of Health Sciences Ben-Gurion University of the Negev Beer-Sheva Israel Department of Obsteirics Gynecology and Reproductive Sciences Univeisity of Pittsburgh School of Medicine Magee-Womens Research InstitutePittsburgh PA 15213 USA

In vitro culture of spermatogonial stem cells （SSCs） has generally been performed using two-dimensional （2D） culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules （including those generated by the SSC niche） and necessary for spermatogenesis. Recently, one of our laboratories has been able to induce proliferation and differentiation of mouse testicular germ cells to meiotic and postmeiotic stages including generation of sperm in a 3D soft agar culture system （SACS） and a 3D methylcellulose culture system （mCS）. It was suggested that SACS and mCS form a special 3D microenvironment that mimics germ cell niche formation in the seminiferous tubules, and thus permits mouse spermatogenesis in vitro. In this review, we （1） provide a brief overview of the differences in spermatogenesis in rodents and primates, （2） summarize data related to attempts to generate sperm in vitro, （3） report for the first time formation of colonies/clusters of cells and differentiation of meiotic （expression of CREm-1） and postmeiotic （expression of acrosin） germ cells from undifferentiated spermatogonia isolated from the testis of prepubertal rhesus monkeys and cultured in SACS and mCS, and （4） indicate research needed to optimize 3D systems for in vitro primate spermatogenesis and for possible future application to man.

关键词： in vitrospermatogenesis methylcellulose culture system monkey primates soft agar culture system three-dimensional culture system

Reducing Value Chain Vulnerability to Terrorist Attacks

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化工进展 2005年第6期24卷 697-702页

作者： A.m.(tony) Downes FmC Corporation Princeton NJ 08543

Like most U.S. chemical companies, you probably know where your security-sensitive inventories are located within your sites and have assured yourself that you have taken reasonable precautions to reduce the risk of their being used in a terrorist attack. What about when you ship them? How secure are the various parts of your value chain-offsite storage and transportation of your products, raw materials, intermediates, and wastes, and processing of ordering and invoicing transactions? Value chain security focuses on tampering and misuse of materials handled outside the plant *** FmC, we took a three-stage approach to identifying and dealing with potential security issues posed by materials in transit. Stage 1 was a quick review to determine which of our products, intermediates, or raw materials might be potentially useful to terrorists. In Stage 2 we looked for specific security-emergency scenarios that might involve those chemicals, estimated the risk of those scenarios, and made recommendations to reduce the vulnerability to terrorist attack. In Stage 3 we are implementing the plans we ***’s approach has successfully focused our efforts and used familiar techniques to break down complex Value Chains into manageable sections and then to identify the possible scenarios and (relative) risks at each section. We were able to do this with minimal travel *** paper describes the general organization of FmC’s value chain security efforts and our analysis technique, and discusses lessons (including a few surprises) that we have learned from the analyses. 2005 American Institute of Chemical Engineers Process Saf Prog 24: 6-11, 20051. ORGANIZATION (FORmAL AND INFORmAL) OF SECURITY ACTIVITIESShortly after the terrorist attack on September 11, 2001, FmC Corporation formed a Transportation Secu-

关键词：化工企业安全管理安全生产价值链

Clinical outcomes for men with positive surgical margins after radical prostatectomy—results from the South Australian Prostate Cancer Clinical Outcomes Collaborative community-based registry

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Asian Journal of Urology 2023年第4期10卷 502-511页

作者： Kerri R.Beckmann michael E.O'callaghan Andrew D.Vincent Kim L.moretti Nicholas R.Brook Cancer Epidemiology and Population Health Research University of South AustraliaAdelaideAustralia Translational Oncology and Urology Research Division of Pharmaceutical and Cancer StudiesKings College LondonLondonUK South Australian Prostate Cancer Clinical Outcomes Collaborative Flinders Medical CentreAdelaideAustralia The Freemason's Foundation Centre for men's Health Adelaide Medical SchoolUniversity of AdelaideAdelaideAustralia Discipline of Surgery University of AdelaideAdelaideAustralia Department of Urology Royal Adelaide HospitalAdelaideAustralia

Objective Positive surgical margins(PSms)after radical prostatectomy(RP)indicate failure of surgery to completely clear *** confer an increased risk of biochemical recurrence(BCR),but how more robust outcomes are affected is *** study investigated factors associated with PSms following RP and determined their impact on clinical outcomes(BCR,second treatment[radiotherapy and/or androgen deprivation therapy],and prostate cancer-specific mortality[PCSm]).methods The study cohort included men diagnosed with prostate cancer(pT2-3b/N0/m0)between January 1998 and June 2016 who underwent RP from the South Australian Prostate Cancer Clinical Outcomes Collaborative *** associated with risk of PSms were identified using Poisson *** impact of PSms on clinical outcomes(BCR,second treatment,and PCSm)was assessed using competing risk *** Of the 2827 eligible participants,28%had PSms—10%apical,6%bladder neck,17%posterolateral,and 5%at multiple *** follow-up was 9.6 years with 81 deaths from prostate cancer *** of PSm increased with higher pathological grade and pathological tumor stage,and greater tumour volume,but decreased with increasing surgeon volume(odds ratio[OR]:0.93;95%confidence interval[CI]:0.88-0.98,per 100 previous prostatectomies).PSms were associated with increased risk of BCR(adjusted sub-distribution hazard ratio[sHR]2.5;95%CI 2.1-3.1)and second treatment(sHR 2.9;95%CI 2.4-3.5).Risk of BCR was increased similarly for each PSm location,but was higher for multiple margin *** found no association between PSms and *** Our findings support previous research suggesting that PSms are not independently associated with PCSm despite strong association with *** PSm rates remains an important objective,given the higher likelihood of secondary treatment with associated comorbidities.

关键词： Prostate cancer Positive surgical margin Biochemical recurrence Radical

Update on hepatocellular carcinoma: Pathologists' review

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World Journal of Gastroenterology 2019年第14期25卷 1653-1665页

作者： tony El Jabbour Stephen m Lagana Hwajeong Lee Department of Pathology and Laboratory medicine Albany Medical College Department of Pathology and Cell Biology College of Physicians and Surgeons Columbia University

Histopathologic diversity and several distinct histologic subtypes of hepatocellular carcinoma(HCC) are well-recognized. Recent advances in molecular pathology and growing knowledge about the biology associated with distinct histologic features and immuno-profile in HCC allowed pathologists to update classifications. Improving sub-classification will allow for more clinically relevant diagnoses and may allow for stratification into biologically meaningful subgroups. Therefore, immuno-histochemical and molecular testing are not only diagnostically useful, but also are being incorporated as crucial components in predicting prognosis of the patients with HCC. Possibilities of targeted therapy are being explored in HCC, and it will be important for pathologists to provide any data that may be valuable from a theranostic perspective. Herein, we review and provide updates regarding the pathologic sub-classification of *** diagnostic approach and the role of biomarkers as prognosticators are reviewed. Further, the histopathology of four particular subtypes of HCC:Steatohepatitic, clear cell, fibrolamellar and scirrhous-and their clinical relevance, and the recent consensus on combined HCC-cholangiocarcinoma is summarized. Finally, emerging novel biomarkers and new approaches to HCC stratification are reviewed.

关键词： Hepatocellular carcinoma Subtype Classification Review Update