检索结果-南通市图书馆

Genetic Background Influences Brassinosteroid-Related Mutant Phenotypes in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第2期4卷 212-221页

作者： tomoaki sakamoto Hidemi Kitano Shozo Fujioka Bioscience and Biotechnology Center Nagoya University Nagoya Japan Faculty of Bioresources and Environmental Sciences Ishikawa Prefectural University Nonoichi Japan RIKEN Advanced Science Institute Wako Japan

In two cases, mutations in the same brassinosteroid-related genes caused different phenotypes in japonica varieties Nipponbare and Taichung 65. The mutant phenotypes were less severe in the Taichung 65 background than in the Nipponbare background. Three newly isolated brassinosteroid-insensitive mutants (d61-1N, d61-11, and d61-12) derived from a Nipponbare mutant library were found to be alleles of d61, which represent defects in the OsBRI1 gene. Although the Nipponbare-derived mutant d61-1N had the same nucleotide substitution as the previously characterized Taichung 65-derived mutant d61-1T, these two mutants showed different phenotypes for plant stature, internode elongation pattern, and seed shape;in each case, d61-1N (in the Nipponbare genetic background) had the more severe mutant phenotype. Similar trends were seen for phenotypes caused by mutants of d2, a brassinosteroid biosynthesis gene. Consistent with these phenotypes, the expression of brassinosteroid-responsive genes was lower in the Nipponbare-derived mutants. These results can be explained by our findings that feed-forward up-regulation of OsBRI1 did not occur in the Nipponbare-derived mutants and that an mPing transposon is inserted into the promoter region of Nipponbare OsBRI1. Based on these results, we conclude that the expression of OsBRI1, especially its feed-forward up-regulation, is misregulated in wild-type Nipponbare and in brassinosteroid-related mutants in a Nipponbare genetic background. Although Nipponbare is a model rice genotype, it can be categorized as an OsBRI1 mutant that has reduced sensitivity to brassinosteroid.

关键词： Brassinosteroid Brassinosteroid Receptor Brassinosteroid Sensitivity miniature Ping (mPing) Mutant Nipponbare Rice

Combination of genetic analysis and ancient literature survey reveals the diverge nee of traditional Brassica rapa varieties from Kyoto, Japan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 1877-1886页

作者： Yaichi Kawakatsu tomoaki sakamoto Hokuto Nakayama Kaori Kaminoyama Kaori Igarashi Masaki Yasugi Hiroshi Kudoh Atsushi JNagano Kentaro Yano Nakao Kubo Michitaka Notaguchi Seisuke Kimura Faculty of Life Sciences Kyoto Sangyo UniversityKamigamo-motoyamaKita-kuKyoto 603-8555Japan Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Center for Plant Sciences Kyoto Sangyo UniversityKamigamo-motoyamaKita-kuKyoto 6038555Japan Graduate School of Science The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan School of Agriculture Meiji University1-1-1Higashi-MitaTama-kuKawasakiKanagawa 214-8571Japan Faculty of Engineering Utsunomiya University7-1-2 YotoUtsunomiyaTochigi 321-8585Japan Center for Ecological Research Kyoto UniversityHirano 2-509-3OtsuShiga 520-2113Japan Faculty of Agriculture Ryukoku University1-5 YokotaniSeta Oe-choOtsuShiga 520-2194Japan Graduate School of Life and Environmental Sciences Kyoto Prefectural University1-5 Hangi-choShimogamoSakyo-kuKyoto 606-8522Japan Biotechnology Research Department Kyoto Prefectural AgricultureForestry and Fisheries Technology Center74 OjiKitainayazumaSeika-choSoraku-gunKyoto 6190244Japan Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Institute of Transformative Bio-Molecules Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan

Since ancient times,humans have bred several plants that we rely on ***,little is known about the divergence of most of these *** the present study,we investigated the divergence of Mibuna(Brassica rapa *** ***),a traditional leafy vegetable in Kyoto(Japan),by combining genetic analysis and a survey of ancient *** is considered to have been bred 200 years ago from Mizuna,another traditional leafy vegetable in *** has simple spatulate leaves,whereas Mizuna has characteristic serrated *** quantitative trait loci(QTL)and gene expression analyses suggested that the downregulation of BrTCP15 expression contributed to the change in the leaf shape from serrated to simple ***,the SNP analysis indicated that the genomic region containing the BrTCP15 locus was transferred to Mibuna by ***,we conducted a survey of ancient literature to reveal the divergence of Mibuna and found that hybridization between Mizuna and a simple-leaved turnip might have occurred in the ***,the genomic analysis of multiple turnip cultivars showed that one of the cultivars,Murasakihime,has almost the same sequence in the BrTCP15 region as *** results suggest that the hybridization between Mizuna and turnip has resulted in the establishment of Mibuna.

关键词： cultivar survey analysis

New Alleles of Rice ebisu dwarf(d2) Mutant Show both Brassinosteroid-Deficient and -Insensitive Phenotypes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2012年第12期3卷 1699-1707页

作者： tomoaki sakamoto Yoichi Morinaka Hidemi Kitano Shozo Fujioka Bioscience and Biotechnology Center Nagoya University Nagoya Japan Faculty of Bioresources and Environmental Sciences Ishikawa Prefectural University Nonoichi Japan RIKEN Advanced Science Institute Wako Japan

ebisu dwarf (d2) is a mutant caused by mutation in a rice brassinosteroid biosynthetic enzyme gene, CYP90D2/D2, thereby conferring a brassinosteroid-deficient dwarf phenotype. Three newly isolated d2 alleles derived from a Nippon- bare mutant library (d2-3, d2-4, and d2-6) produced more severe dwarf phenotypes than the previously characterized null allele from a Taichung 65 mutant library, d2-1. Linkage analysis and a complementation test clearly indicated that the mutant phenotypes in d2-6 were caused by defects in CYP90D2/D2, and exogenous treatment with brassinolide, a bioactive brassinosteroid, rescued the dwarf phenotype of three Nipponbare-derived d2 mutants. However, the content of endogenous bioactive brassinosteroid, castasterone, and the expression of brassinosteroid-response genes indicated that partial suppression of the brassinosteroid response in addition to a brassinosteroid deficiency has occurred in the Nipponbare-derived d2 mutants. Based on these results, we discuss the possibility that wild-type Nipponbare has some defects in an unknown factor or factors related to the brassinosteroid response in rice.

关键词： Brassinosteroids Brassinosteroid Biosynthetic Enzymes Brassinosteroid Sensitivity Cytochrome P450 Monooxygenase Mutant Allele Rice

Effects of Abscisic Acid Treatment on Berry Coloration and Expression of Flavonoid Biosynthesis Genes in Grape

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2016年第9期7卷 1325-1336页

作者： Ayako Katayama-Ikegami tomoaki sakamoto Kana Shibuya Takane Katayama Mei Gao-Takai Faculty of Bioresources and Environmental Sciences Ishikawa Prefectural University Ishikawa Japan Graduate School of Biostudies Kyoto University Kyoto Japan

In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. Exogenous ABA treatment increased anthocyanin content, especially petunidin- and malvidin-type anthocyanins. Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. Thus, increases in the total amount and composition ratios of petunidin- and malvidin-type anthocyanins were mainly caused by ABA-induced upregulation of uridine diphosphate glucose flavonoid glucosyl transferase, glutathione S-transferase 4, O-methyl transferase and flavonoid 3’, 5’ hydroxylase expression, resulting in the deep coloration of berry of skin.

关键词： Abscisic Acid (ABA) Anthocyanin Biosynthetic Enzyme Gene Expression Transcription Factor

Mercury concentration in hair samples from Chinese people in coastal cities

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2008年第10期20卷 1258-1262页

作者： sakamoto Mineshi National Institute for Minamata Disease Ministry of the Environment4058-18 Hama Minamata City Kumamoto 867-0008 Japan.

This investigation was made to estimate current normal concentrations of total mercury in the hair of Chinese coastal people. Hair samples were collected from 659 healthy inhabitants in the areas along the coast and the rivers (such as Shanghai, Ningbo, Dalian, Xiamen, and Zhoushan) of China from Feb. 2005 to June 2006. Total mercury concentrations in the samples were analyzed by the cold vapor atomic adsorption spectrometry method (CVAAS). The results showed the geometric mean concentration of total mercur...

关键词： hair mercury Chinese people coastal cities

Saline Drinking Water and Salt in Diet:An Approximate Picture of the Situation in a Coastal Area of Southeastern Bangladesh

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Disaster Risk Science 2017年第2期8卷 109-120页

作者： Maiko sakamoto Department of International Studies Graduate School of Frontier SciencesThe University of Tokyo

Coastal areas of Bangladesh have been facing increasing salinity of surface water and *** study provides the approximate scope of the problem in a coastal area of southeastern Bangladesh by using less-accurate,but lower-cost salinity measuring devices that enable local people to assess the *** local women were employed to monitor 10 tube wells each on a weekly basis,from mid-February to mid-May,during the2016 dry *** Information System and time series clustering were used to visualize the spatial distribution and seasonal change of the salinity *** addition,the tube well users were asked about the salt consumption in their daily ***-third of the monitored tube wells were found to contain more sodium than the tolerable level in terms of taste suggested by the World Health ***,the mean salinity level across all monitored tube wells was much *** salinity level varied depending on the depth of the tube wells rather than their locations or altitudes,and those deeper than 200 m were likely to be salt *** results of the diet survey showed that wealthier households tended to use more salt in their daily diets,but at the same time they tended to have deeper tube wells that are less likely to contain high levels of sodium.

关键词： Bangladesh Climate change Drinking water salinity Participatory water monitoring

Life Cycle Assessment of Biodegradable Plastics

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Shanghai Jiaotong university(Science) 2012年第3期17卷 327-329页

作者： sakamoto Yuki Research Institute of Public Affairs Nara Sangyo UniversityNara 636-8503Japan

Many industrial products made from fossil fuels cause the problems of global warming,depletion of fossil fuel resources and decrease of the land by *** this report,the amounts of CO2 emissions as the environmental load and the specific energy consumptions as the depletion of fossil fuel resources of biodegradable plastics and conventional plastic as food trays are compared using life cycle assessment *** amount of CO2 emission of PHB(poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) tray was the smallest among those of trays of other *** specific energy consumption of SPCL(starch/polycaprolactone) was the smallest among those of trays of other *** clearly reveals that the utilization of the biodegradable plastics is an effective way of alternatives of the conventional plastics.

关键词： life cycle assessment biodegradable plastics CO2 emission specific energy consumption

Relationship between testicular volume and testicular function： comparison of the Prader orchidometric and ultrasonographic measurements in patients with infertility

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2008年第2期10卷 319-324页

作者： Hideo sakamoto Yoshio Ogawa Hideki Yoshida Department of Urology Showa University School of Medicine Tokyo 142-8666 Japan

Aim： To evaluate the relationship between testicular function and testicular volume measured by using Prader orchidometry and ultrasonography （US） to determine the critical testicular volume indicating normal testicular function by each method. Methods： Total testicular volume （right plus left testicular volume） was measured in 794 testes in 397 men with infertility （mean age, 35.6 years） using a Prader orchidometer and also by ultrasonography. Ultrasonographic testicular volumes were calculated as length x width x height x 0.71. To evaluate volume-function relationships, patients were divided into 10 groups representing 5-mL increments of total testicular volume by each method from below 10 mL to 50 mL or more. Results： Mean total testicular volume based on Prader orchidometry and US were 36.8 mL and 26.3 mL, respectively. Semen volume, sperm density, total sperm count, total motile sperm count, and serum FSH, LH, and testosterone all correlated significantly with total testicular volume measured by either method. Mean sperm density was in the oligozoospermic range in patients with total testicular volume below 35 mL by orchidometry or below 20 mL by ultrasonography. Mean total sperm count was subnormal in patients with total testicular volume below 30 mL by orchidometry or under 20 mL by ultrasonography. Conclusion： Testicular volume measured by either ultrasonography or Prader orchidometry correlated significantly with testicular function. However, critical total testicular volume indicating normal or nearly normal testicular function was 30 mL to 35 mL using Prader orchidometer and 20 mL using ultrasonography. Prader orchidometry morphometrically and functionally overestimated the testicular volume in comparison to US. （Asian JAndro12008 Mar; 10： 319-324）

关键词： orchidometer ultrasonography testicular volume testicular function

Essentials of Proteolytic Machineries in Chloroplasts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2017年第1期10卷 4-19页

作者： Kenji Nishimura Yusuke Kato Wataru sakamoto Institute of Plant Science and Resources Okayama University 2-20-1 Chuo Kurashiki Okayama 710-0046 Japan

Plastids are unique organelles that can alter their structure and function in response to environmental and developmental stimuli. Chloroplasts are one type of plastid and are the sites for various metabolic pro- cesses, including photosynthesis. For optimal photosynthetic activity, the chloroplast proteome must be properly shaped and maintained through regulated proteolysis and protein quality control mechanisms. Enzymatic functions and activities are conferred by protein maturation processes involving consecutive proteolytic reactions. Protein abundances are optimized by the balanced protein synthesis and degrada- tion, which is depending on the metabolic status. Malfunctioning proteins are promptly degraded. Twenty chloroplast proteolytic machineries have been characterized to date. Specifically, processing peptidases and energy-driven processive proteases are the major players in chloroplast proteome biogenesis, remod- eling, and maintenance. Recently identified putative proteases are potential regulators of photosynthetic functions. Here we provide an updated, comprehensive overview of chloroplast protein degradation ma- chineries and discuss their importance for photosynthesis. Wherever possible, we also provide structural insights into chloroplast proteases that implement regulated proteolysis of substrate proteins/peptides.

关键词： chloroplast biogenesis protease protein homeostasis