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How do Plants Organize Microtubules Without a Centrosome?

Journal of Integrative Plant Biology 2007年第8期49卷 1154-1163页

作者： TakashiMurata TakakoTanahashi tomoaki nishiyama Kazuo Yamaguchi Mitsuyasu Hasebe National Institute for Basic Biology Okazaki 444-8585Japan Department of Basic BiologyThe Graduate University for Advanced StudiesOkazaki 444-8585Japan Advanced Science Research Center Kanazawa UniversityTakaramachi 13-1Kanazawa 920-0934Japan ERATOJapan Science and Technology AgencyTakaramachi 13-1Kanazawa 920-0934Japan Advanced Science Research Center Kanazawa UniversityTakaramachi 13-1Kanazawa 920-0934Japan Graduate School of Natural Science and TechnologyKanazawa UniversityKanazawa 920-1192Japan National Institute for Basic Biology Okazaki 444-8585Japan Department of Basic BiologyThe Graduate University for Advanced StudiesOkazaki 444-8585Japan ERATOJapan Science and Technology AgencyOkazaki 444-8585Japan

A microtubule nucleates from a γ-tubuUn complex, which consists of γ-tubulin, proteins from the SPC971SPC98 family, and the WD40 motif protein GCP-WD. We analyzed the phylogenetic relationships of the genes encoding these proteins and found that the components of this complex are widely conserved among land plants and other eukaryotes. By contrast, the interphase and mitotic arrays of microtubules in land plants differ from those in other eukaryotes. In the interphase cortical array, the majority of microtubules nucleate on existing microtubules in the absence of conspicuous microtubule organizing centers （MTOCs）, such as a centrosome. During mitosis, the spindle also forms in the absence of conspicuous MTOCs. Both poles of the spindle are broad, and branched structures of microtubules called microtubule converging centers form at the poles. In this review, we hypothesize that the microtubule converging centers form via microtubule-dependent microtubule nucleation, as in the case of the interphase arrays. The evolutionary insights arising from the molecular basis of the diversity in microtubule organization are discussed.

关键词： cortical microtubules evolution γ-tubulin microtubule nucleation mitotic spindle.

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Retrieving structural information from scattering and attenuation data of transparent wood and(Nano)paper

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Journal of Bioresources and Bioproducts 2021年第3期6卷 187-194页

作者： Yoshiharu nishiyama Univ.Grenoble Alpes CNRSCERMAVGrenoble38000France

Over the last 15 years,significant number of reports on transparent paper and transparent wood appeared in the *** light scattering data or transmission data are often given to describe the optical performance of the *** addition,the data also contains structural information that can be further analyzed based on scattering *** of the data are re-analyzed herein from structural analysis point of view related to the scattering *** analysis on the wavelength dependent optical density of nanopaper suggested that the scatterers are not isolated voids or microfibrils but rather large submicrometric and structural *** dependence of transparent wood scattering suggests the scattering units of a few micrometers such as cell wall are at the origin of high haze.

关键词： Light scattering Transparent wood Nanocellulose

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Experimental aspects of ^(14)N overtone RESPDOR solid-state NMR spectroscopy under MAS beyond 60 kHz

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Magnetic Resonance Letters 2024年第1期4卷 40-49页

作者： Yutaro Ogaeri Yusuke nishiyama JEOL Ltd. AkishimaTokyo196-8558Japan

Nitrogen-14(^(14)N)overtone(OT)spectroscopy under fast magic angle spinning(MAS)conditions(>60 kHz)has emerged as a powerful technique for observing correlations and distances between ^(14)N and ^(1)H,owing to the absence of the first-order quadrupolar broad*** addition,^(14)N^(OT) allows selective manipulation of ^(14)N nuclei for each *** extensive theoretical and experimental studies,the spin dynamics of ^(14)N^(OT) remains under *** this study,we conducted experimental investigations to assess the spin dynamics of ^(14)N^(OT) using the rotational-echo saturation-pulse double-resonance(RESPDOR)sequence,which monitors population transfer induced by a^(14)N^(OT) *** ^(14)N^(OT) spin dynamics is well represented by a model of a two-energy-level *** spin-1/2,the maximum excitation efficiency of ^(14)N^(OT) coherences of powdered solids,denoted by p,depends on the radiofrequency field(rf-field)strength due to orientation dependence of effective nutation fields even when pulse lengths are *** is also found that the p factor,contributing to the ^(14)N^(OT) spin dynamics,is nearly independent of the B0 ***,the filtering efficiency of RESPDOR experiments exhibits negligible dependence on B0 when the ^(14)N^(OT) pulse length is *** study also identifies the optimal experimental conditions for ^(14)N^(OT)/^(1)H RESPDOR correlation experiments.

关键词： ^(14)N Overtone RESPDOR ^(14)N/^(1)H correlation Solid-state NMR Fast MAS

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Model Analysis of Radar Echo Split Observed in an Artificial Cloud Seeding Experiment

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Journal of Meteorological Research 2016年第3期30卷 386-400页

作者： MASAKI Shimada KIKURO Tomine KOJI nishiyama Department of Earth and Ocean Sciences National Defense Academy Faculty of Engineering Kyushu University

An artificial cloud seeding experiment was performed over the Japan Sea in winter to show how massive seeding could be effective to mitigate heavy snowfall *** results showed that 20 min after cloud seeding,a portion of the radar echo beneath the seeding track was weakened to divide the radar echo into two *** order to analyze the results,a numerical simulation was conducted by using the Weather Research and Forecasting model verion *** this simulation,the seeding effects were represented as phenomena capable of changing rain particles by accreting cloud ice and snow to form graupel particles and by changing cloud liquid water to snow *** graupel particles fell rapidly,thus temporarily intensifying the rainfall,which subsequently ***,the weakened radar echo in the field experiment is deemed to have been caused by the increase in rapidly falling graupel particles.

关键词： heavy snowfall artificial cloud seeding numerical experiment

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Benefit of Selecting Number of Active Mesh Routers in Disaster Oriented Wireless Mesh Network

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Journal of Software Engineering and Applications 2012年第12期5卷 36-41页

作者： Panu Avakul Hiroki nishiyama Nei Kato Yoshitaka Shimizu tomoaki Kumagai Graduate School of Information Sciences Tohoku UniversitySendai Japan 2NTT Network Innovation Laboratories NTT Corporation Yokosuka Japan

Communications is one of the most critical factors in disaster recovery process. However, after a major disaster, existing communications infrastructures may be heavily damaged or even completely unusable. It is necessary that communicationsare to be promptly restored to the disaster area, which is the goal of our national project. The project aims to build three tiers wireless mesh network from remaining wireless access points in order to provide communications services to the disaster area. This work introduces a unique multiple tiers wireless mesh network project. In addition, this work also illustrates merits in optimizing the number of mesh routersin order to achieve the optimum performance by presenting both theoretical and simulation results of a specific scenario of multiple tiers wireless mesh network.

关键词： Wireless Mesh Network Throughput Interference Collision WiFi

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Numerical Modelling and Simulation of Chemical Reactions in a Nano-Pulse Discharged Bubble for Water Treatment

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Plasma Science and Technology 2016年第9期18卷 924-932页

作者：何雨辰 Satoshi UEHARA Hidemasa TAKANA Hideya nishiyama Graduate school of Engineering Tohoku University Institute of Fluid Science Tohoku University

A zero-dimensional model to simulate a nano-pulse-discharged bubble in water was developed. The model consists of gas and liquid phases corresponding to the inside and outside of the bubble, respectively. The diffusions of chemical species from the gas to the liquid phase through the bubble interface was also investigated. The initial gas is Ar, but includes a little H20 and 02 in the bubble. The time evolution of the OH concentration in the liquid phase was mainly investigated as an important species for water treatment. It was shown that OH was generated in the bubble and then diffused into the liquid. With the application of a continuous nano-pulse discharge, more OH radicals were generated as the frequency increased at a low voltage for a given power consumption.

关键词： chemical reaction model water purification bubble discharge diffusion

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An Example of Canal Formation in a Thick Cloud Induced by Massive Seeding Using Liquid Carbon Dioxide

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Journal of Meteorological Research 2015年第4期29卷 682-690页

作者： KIKURO Tomine MASAKI Shimada KENJI Wakimizu KOJI nishiyama Department of Earth and Ocean Sciences National Defense Academy Faculty of Agriculture Kyushu University Hakozaki Faculty of Engineering Kyushu University Motooka

The purpose of this experiment is to show that massive cloud seeding is effective in mitigating the damage caused by heavy snowfall. In order to show its effect, we attempted to form a canal in a thick convective cloud by massive seeding, and left the parts that were not influenced by the seeding as a reference to show that the canal was formed by the massive seeding only. The seeding was carried out by using an aircraft. The seeding rate and air speed of the aircraft were 35 g s-1 and 115 m s 1, respectively. The flight course for seeding was selected to be parallel to the wind direction to ensure that the dispersed liquid carbon dioxide did not influence both sides of the course. The results show that a part of the radar echo observed from onboard beneath the seeding track was weakened and divided the radar echo into two parts 20 minutes after the cloud top and the bottom were seeded, and distribution of rainfall rate on the ground from the target cloud was confirmed to be divided into two parts 24 minutes after the seeding. The target cloud was torn along the seeding track, and we could see the sea surface through the break in the cloud. Canal formation occurred in the cloud along the seeding track. Clouds and snowfall were left on both sides of the canal. The results show that supercooled liquid cloud particles along the seeding track evaporated to form larger precipitable particles which grew and fell rapidly.

关键词： heavy snowfall weather modification aircraft artificial cloud seeding

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Scattering Characteristics of Liquid Droplets Spun Off from Rotating Disk Edge

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Journal of Thermal Science 2012年第1期21卷 42-48页

作者： Mizue MUNEKATA Akira NOGUCHI Jun nishiyama Hiroaki KURISHIMA Hiroyuki YOSHIKAWA Dept. of Mech. System Eng. Kumamoto University 2-39-1 Kurokami Tokyo Electron Kyushu Ltd. Fukuhara

Scattering characteristics of liquid droplets spun off from a rotating disk edge are experimentally investigated. In the present research, aluminum disks are utilized and ethanol is employed for liquid. Scattering phenomena of the droplets are captured by the high-speed digital camera. Frequency distribution of the droplet diameter is evaluated from these images and distributions of horizontal flying velocity and angle of the droplets were measured by PTV. Liquid filaments are stretched outward from the stagnant liquid layer by centrifugal force and skew complicatedly by aerodynamic force. Some peaks appear in the distribution of the scattered droplet diameter and they are origi- nated from large terminal droplets and small droplets generated from filamentwise breakup. Most of the scattered droplets fly slightly inside in the tangential direction of the disk edge. The droplets spun off from the thin disk scatter widely compared with that from the thick one.

关键词： Rotating disk Scattering droplet Visualization Particle tracking velocimetry

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Technical Development of Simple Shear Deformation Experiments Using a Deformation-DIA Apparatus

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Journal of Earth Science 2010年第5期21卷 523-531页

作者： Tomohiro Ohuchi Takaaki Kawazoe Norimasa nishiyama Yu Nishihara Tetsuo Irifune Geodynamics Research Center Ehime University Senior Research Fellow Center Ehime University

Technical developments for simple shear deformation experiments at high pressures were made. The newly designed cell assembly can be compressed by deformation-DIA apparatuses with the MA 6-6 system, which consists of six second-stage tungsten carbide anvils （with a truncated edge length of 5 mm） and the anvil guide. Deformation of samples was barely observed during the compression process, showing that the shear strain of the deformed samples can be measured by the rotation of a strain marker. Simple shear deformation experiments on anhydrous and hydrous oli- vine aggregates were conducted under upper mantle conditions （pressures of 5.2-7.6 GPa and temperatures of 1 473-1 573 K）, and sample deformation with a shear strain of 7=0.8-1.2 was successfully achieved at a shear strain rate of 4.0×10^-5-7.5×10^-5 s^-1. The present study extended the pressure range of simple shear deformation experiments in the deformation-DIA apparatus from 3 GPa in an early study to 7.6 GPa at high temperatures.

关键词： simple shear deformation deformation-DIA MA 6-6 upper mantle.

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Preliminary Deformation Experiment of Ringwoodite at 20 GPa and 1 700 K Using a D-DIA Apparatus

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Journal of Earth Science 2010年第5期21卷 517-522页

作者： Takaaki Kawazoe Tomohiro Ohuchi Norimasa nishiyama Yu Nishihara Tetsuo Irifune Geodynamics Research Center Ehime University Senior Research Fellow Center Ehime University

A deformation experiment of ringwoodite with a strain of 9% was achieved at 20 GPa and 1 700 K and at a strain rate of 3×10^-5 s^-1 using a deformation-DIA （D-DIA） apparatus and a multi-anvil 6-6 （MA 6-6） assembly. The crystallographic orientations of the deformed sample were successfully analyzed by the electron backscatter diffraction （EBSD） method, although any notable latticepreferred orientation （LPO） was not observed presumably due to the insufficient strain in the present experiment. In this study, the deformation experiment on ringwoodite succeeded at P-T conditions consistent with the lower part of the mantle transition zone and at a controlled strain rate for the first time. The present study extended the pressure range of deformation experiments in the D-DIA apparatus from 16 GPa in our earlier study to 20 GPa at 1 700 K. The successful extension of the pressure range demonstrates potential importance of the D-DIA apparatus in studying rheological properties of minerals under the P-T conditions of the whole mantle transition zone.

关键词： ringwoodite mantle transition zone rheology viscosity mantle dynamics.

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