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Self-replicating digital data storage with synthetic chromosomes

National Science Review 2021年第7期8卷 66-67页

作者： Xinyu Lu tom ellis Imperial College Centre for Synthetic Biology Imperial College London Department of Bioengineering Imperial College London Wellcome Trust Sanger Institute

With the rapid development of the Internet and computational technologies, the exponential growth of digital data is beginning to overwhelm existing storage device capacities, driving an unprecedented demand for new data storage media. DNA, a natural genetic information storage system, has been proposed as a promising alternative[1]. Compared with traditional siliconbased storage, DNA-based storage hasmany advantages—incredible density,

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Propagation of Recombinant Genes through Complex Microbiomes with Synthetic Mini-RP4 Plasmid Vectors

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BioDesign Research 2022年第1期2022卷 193-207页

作者： tomás Aparicio Jillian Silbert Sherezade Cepeda Víctor de Lorenzo Systems and Synthetic Biology Department Centro Nacional de Biotecnología(CNB-CSIC)Campus de Cantoblanco Madrid 28049Spain

The promiscuous conjugation machinery of the Gram-negative plasmid RP4 has been reassembled in a minimized, highlytransmissible vector for propagating genetically encoded traits through diverse types of naturally occurring microbialcommunities. To this end, the whole of the RP4-encoded transfer determinants (tra, mob genes, and origin of transfer oriT)was excised from their natural context, minimized, and recreated in the form of a streamlined DNA segment borne by anautoselective replicon. The resulting constructs (the pMATING series) could be self-transferred through a variety ofprokaryotic and eukaryotic recipients employing such a rationally designed conjugal delivery device. Insertion of GFP reporterinto pMATING exposed the value of this genetic tool for delivering heterologous genes to both specific mating partners andcomplex consortia (e.g., plant/soil rhizosphere). The results accredited the effective and functional transfer of the recombinantplasmids to a diversity of hosts. Yet the inspection of factors that limit interspecies DNA transfer in such scenarios uncoveredtype VI secretion systems as one of the factual barriers that check otherwise high conjugal frequencies of tested RP4derivatives. We argue that the hereby presented programming of hyperpromiscuous gene transfer can become a phenomenalasset for the propagation of beneficial traits through various scales of the environmental microbiome.

关键词： otherwise rational transferred

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