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Qualification of Soybean Responses to Flooding Stress Using UAV-Based Imagery and Deep Learning

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Plant Phenomics 2021年第1期3卷 365-377页

作者： Jing Zhou huawei Mou Jianfeng Zhou Md Liakat Ali heng ye Pengyin Chen henry t.Nguyen Division of Food Systems and Bioengineering University of MissouriColumbiaMO 65211USA Bioenergy and Environment Science&technology Laboratory College of EngineeringChina Agricultural UniversityBeijing 100083China Fisher Delta Research Center University of MissouriPortagevilleMO 63873USA Division of Plant Sciences University of MissouriColumbiaMO 65211USA

Soybean is sensitive to flooding stress that may result in poor seed quality and significant yield *** production under flooding could be sustained by developing flood-tolerant cultivars through breeding ***,soybean tolerance to flooding in field conditions is evaluated by visually rating the shoot injury/damage due to flooding stress,which is labor-intensive and subjective to human *** developments of field high-throughput phenotyping technology have shown great potential in measuring crop traits and detecting crop responses to abiotic and biotic *** goal of this study was to investigate the potential in estimating flood-induced soybean injuries using UAV-based image features collected at different flight *** flooding injury score(FIS)of 724 soybean breeding plots was taken visually by breeders when soybean showed obvious injury *** images were taken on the same day using a five-band multispectral and an infrared(IR)thermal camera at 20,50,and 80 m above *** image features,i.e.,canopy temperature,normalized difference vegetation index,canopy area,width,and length,were extracted from the images at three flight.heights.A deep learning model was used to classify the soybean breeding plots to five FIS ratings based on the extracted image *** show that the image features were significantly different at three flight *** best classification performance was obtained by the model developed using image features at 20 m with 0.9 for the five-level *** results indicate that the proposed method is very promising in estimating FIS for soybean breeding.

关键词： Deep image breeding

Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and t-type calcium channels

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Neuroscience Bulletin 2012年第6期28卷 715-722页

作者： ye-Nan Chi Xu Zhang Jie Cai Feng-Yu Liu Guo-Gang Xing You Wan Neuroscience Research Institute and Department of Neurobiology Peking University Key Laboratory for Neuroscience Ministry of Education /Ministry of HealthPeking University

Objective Formaldehyde at.high concentrations is a contributor to air pollution. It is also an endogenous metabolic product in cells, and when beyond physiological concentrations, has pathological effects on neurons. Formaldehyde induces mis-folding and aggregation of neuronal tau protein, hippocampal neuronal apoptosis, cognitive impairment and loss of memory functions, as well as excitation of peripheral nociceptive neurons in cancer pain models. Intracellular calcium （[Ca2＋]i） is an important intracellular messenger, and plays a key role in many pathological processes. the present study aimed to investigate the effect of formaldehyde on [Ca2＋]i and the possible involvement of N-methyl-D-aspartate receptors （NMDARs） and t-type Ca2＋ channels on the cell membrane. Methods Using primary cultured hippocampal neurons as a model, changes of [Ca2＋]i in the presence of formaldehyde at a low concentration were detected by confocal laser scanning microscopy. Results Formaldehyde at 1 mmol/L approximately doubled [Ca2＋]i. （2R）-amino-5-phosphonopentanoate （AP5, 25 μmol/L, an NMDAR antagonist） and mibefradil （MIB, 1 μmol/L, a t-type Ca2＋ channel blocker）, given 5 min after formaldehyde perfusion, each partly inhibited the formaldehyde-induced increase of [Ca：＋]i, and this inhibitory effect was reinforced by combined application of AP5 and MIB. When applied 3 min before formaldehyde perfusion, AP5 （even at 50μmol/L） did not inhibit the formaldehyde-induced increase of [Ca2＋]i, but MIB （1 μmol/L） significantly inhibited this increase by 70%. Conclusion these results suggest that formaldehyde at a low concentration increases [Ca2＋]i in cultured hippocampal neurons; NMDARs and t-type Ca2＋ channels may be involved in this process.

关键词： formaldehyde intracellular calcium neuronal activation NMDA receptors t-type calcium channels

the use of the node-based smoothed finite element method to estimate static and seismic bearing capacities of shallow strip footings

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第1期14卷 180-196页

作者： h.C.Nguyen t.Vo-Minh Department of civil and environmental engineering imperial college londonuk Department of civil engineering and industrial design university of liverpoolliverpooluk Faculty of civil engineering ho chi minh city university of technology(hutech)ho chi minh cityviet nam

the node-based smoothed finite element method(NS-FEM)is shortly presented for calculations of the static and seismic bearing capacities of shallow strip footings.A series of computations has been performed to assess variations in seismic bearing capacity factors with both horizontal and vertical seismic *** results obtained agree very well with those using the slip-line method,revealing that the magnitude of the seismic bearing capacity is highly dependent upon the combinations of various directions of both components of the seismic *** upward vertical seismic acceleration reduces the seismic bearing capacity compared to the downward vertical seismic acceleration in *** addition,particular emphasis is placed on a separate estimation of the effects of soil and superstructure inertia on each seismic bearing capacity *** the effect of inertia forces arising in the soil on the seismic bearing capacity is non-trivial,and the superstructure inertia is the major contributor to reductions in the seismic bearing *** tables and charts are given for practical application to the seismic design of the foundations.

关键词： Limit analysis Node-based smoothed finite element method(NS-FEM) Second-order cone programming(SOCP) Seismic bearing capacity Strip footing

Optimal mother wavelet-based Lamb wave analyses and damage detection for composite structures

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仪器仪表学报 2007年第10期28卷 1729-1735页

作者： Li Fucai Meng Guang ye Lin State Key Laboratory of Mechanical System and Vibration Shanghai Jiao Tong University Shanghai 200240 China School of Aerospace Mechanical ＆ Mechatronic Engineering The University of Sydney NSW 2006 Australia

With the purpose of on-line structural health monitoring,a transducer network was embedded into compos- ite structure to minimize the influence of *** intrinsic dispersion characteristic of Lamb wave makes the wavelet transform an effective signal processing method for guided *** get.high precision in feature extrac- tion,an information entropy-based optimal mother wavelet selection approach was proposed,which was used to choose the most appropriate basis function for particular Lamb wave *** using the embedded sensor network and extracting time-of-flight,delamination in the composite laminate was identified and *** results demon- strate the effectiveness of the proposed methods.

关键词：拉姆波损失探测内传感器信息熵最优小波基

DIMENSIONS OF AtACtIC POLY(α-MEthYLStYRENE)ChAINS WIth SIDE GROUPS

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Chinese Journal of Polymer Science 2006年第1期24卷 87-93页

作者： hai-zhu Ma Gao-xiang ye Department of Physics Zhejiang University Hangzhou 310027 China Department of Physics Zhejiang Education Institute Hangzhou 310012 China

An improved configurational-confomational statistical method is developed and the mean-square radius of gyration for atactic poly（α-methylstyrene）（PαMS） chains is studied, in which the effect of large side groups is considered. the deduced formulas, based on the rotational isomer state theory, are used to investigate the configuration-dependent properties of the atactic polymer chain, and the statistical correlation of the unperturbed polymer chain dimension and structure parameters are calculated. For the fraction of meso dyads Wm= 0.4, the dependence of the radius of gyration Rg and the intrinsic viscosity [η] on the molecule mass M are Rg = 2.63×10^-2 M^0.50 nm and [η] = 7.36 × 10^-2 M^0.497, respectively, which are in agreement with the previous experimental data for the PαMS samples. A small hump is detected in the curve of the characteristic ratio of the unperturbed mean-square radius of gyration versus the chain length for short PαMS chains. the Rg increases linearly with the temperature t, and the effects of the chain length and the tacticity on the temperature coefficient are remarkable. these are quite different from the results for PαMS chains not considering side groups or for the monosubstituted polystyrene chain.

关键词： Side groups Rotational isomeric state Radius of gyration Poly(α-methylstyrene) chain.

Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in t2 and t3 generations

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Journal of Integrative Agriculture 2020年第1期19卷 23-32页

作者： LIU hui-yun WANG Ke WANG Jing DU Li-pu PEI Xin-wu ye Xing-guo Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Genetically modified wheat.has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double t-DNAs vector were identified by fluorescence in situ hybridization(FISh) in the t1 generation, and their genetic stability and agronomic traits were analyzed in t2 and t3 generations. FISh analysis indicated that the transgene often integrated into a position at the distal region of wheat chromosomes. Furthermore, we show that the GUS transgene was stably inherited in the marker-free transgenic plants in t1 to t3 generations. No significant differences in agronomic traits or grain characteristics were observed in t3 generation, with the exception of a small variation in spike length and grains per spike in a few lines. the selection marker of bar gene was not found in the transgenic plants through t1 to t3 generations. the results from this investigation lay a solid foundation for the potential application of the marker-free transgenic wheat plants achieved through the co-transformation of double t-DNAs vector by Agrobacterium in agriculture after biosafty evaluation.

关键词： wheat marker-free transgenic plants fluorescence in situ hybridization genetic stability

A critical role of foxp3a-positive regulatory t cells in maintaining immune homeostasis in zebrafish testis development

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Journal of Genetics and Genomics 2020年第9期47卷 547-561页

作者： Xianmei Li Fenghua Zhang Nan Wu Ding ye Yaqing Wang Xiaofan Zhang Yonghua Sun Yong-An Zhang State Key Laboratory of Freshwater Ecology and Biotechnology Innovation Academy for Seed DesignInstitute of HydrobiologyChinese Academy of SciencesWuhan430072China University of Chinese Academy of Sciences Beijing100049China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China State Key Laboratory of Agricultural Microbiology College of FisheriesHuazhong Agricultural UniversityWuhan430070China

Suppressive regulatory t cells(treg cells)play a vital role in preventing autoimmunity and restraining excessive immune response to both self-and *** on humans and mice show that the Forkhead box p3(Foxp3)is a key regulatory gene for the development and function of treg *** zebrafish,treg cells have been identified by using foxp3a as a reliable ***,little is known about the function of foxp3a and treg cells in gonadal development and sex ***,we show that foxp3a is essential for maintaining immune homeostasis in zebrafish testis *** found that foxp3a was specifically expressed in a subset of t cells in zebrafish testis,while knockout of foxp3a led to deficiency of foxp3a-positive treg cells in the *** than 80%of foxp3a^(-/-)mutants developed as subfertile males,and the rest of the mutants developed as fertile females with decreased *** study revealed that foxp3a^(-/-)mutants had a delayed juvenile ovary-to-testis transition in definite males and sex reversal in about.half of the definite females,which led to a dominance of later male *** to the absence of foxp3a-positive treg cells in the differentiating testis of foxp3a^(-/-)mutants,abundant t cells and macrophages expand to disrupt an immunosuppressive milieu,resulting in defective development of germ cells and gonadal somatic cells and leading to development of infertile ***,our study reveals that foxp3a-positive treg cells play an essential role in the orchestration of gonadal development and sex differentiation in zebrafish.

关键词： Foxp3a treg cell Immune homeostasis Gonadal development Zebrafish

Conjugate natural convection of non-Newtonian hybrid nanofluid in wavy-shaped enclosure

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Applied Mathematics and Mechanics(English Edition) 2022年第3期43卷 447-466页

作者： S.hUSSAIN t.tAyeBI t.ARMAGhANI A.M.RAShAD h.A.NABWEY Department of Mathematics Capital University of Science and TechnologyIslamabad 44000Pakistan Lehrst.hl III Department of MathematicsTechnical University of DortmundDortmund 44227Germany Faculty of Sciences and technology University of Bordj Bou ArreridjEl-Anasser 34030Algeria Department of Engineering West Tehran BranchIslamic Azad UniversityTehran 11365Iran Department of Mathematics Aswan UniversityFaculty of ScienceAswan 81528Egypt Department of Mathematics College of Science and Humanities in Al-KharjPrince Sattam Bin Abdulaziz UniversityAl-Kharj 11942Saudi Arabia Department of Basic Engineering Science Faculty of EngineeringMenou¯a UniversityShebin El-Kom 32511Egypt

the present study concerns the modelization and numerical simulation for the heat and flow exchange characteristics in a novel configuration saturated with a nonNewtonian Ag-MgO hybrid *** wavy shaped enclosure is equipped with onequarter of a conducting solid *** system of equations resulting from the mathematical modeling of the physical problem in its dimensionless form is discretized via the higher-order Galerkin-based finite element method(GFEM).the dependency of various factors and their interrelationships affecting the hydro-thermal behavior and heat exchange rate are *** numerical experiments reveal that the best.heat transfer rate is achieved for the pseudo-plastic hybrid nanoliquid with high Rayleigh number and thermal conductivity ratio and low hartmann ***,the power-law index has a major effect in deteriorating the heat convection at.high Rayleigh number.

关键词： natural convection waved-shaped cavity magnetic field hybrid nanofluid higher order finite element method(FEM)

高活性脐带间充质干细胞干预老年树鼩衰老脾脏的作用与机制

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中国组织工程研究 2025年第19期29卷 4000-4010页

作者：叶丽田川赵晓娟陈梦蝶叶倩倩李强廖珠银李晔朱向情阮光萍何志旭舒莉萍潘兴华贵州医科大学基础医学院免疫学教研室组织工程与干细胞实验中心贵州省贵阳市550004 中国人民解放军联勤保障部队第九二〇医院干细胞与免疫细胞生物医药技术国家地方联合工程实验室云南省细胞治疗技术转化医学重点实验室昆明市干细胞与再生医学重点实验室基础医学实验室云南省昆明市650032 遵义医科大学附属医院儿科学教研室贵州省遵义市563100

背景:脾脏具有储血、造血和免疫功能,随着年龄增长,脾脏结构退变、功能衰退引起免疫系统功能受损,进而加速机体衰老进程,而高活性脐带间充质干细胞治疗树鼩脾脏衰老尚未见报道。目的:探讨高活性脐带间充质干细胞对树鼩脾脏衰老的干预作... 详细信息

背景:脾脏具有储血、造血和免疫功能,随着年龄增长,脾脏结构退变、功能衰退引起免疫系统功能受损,进而加速机体衰老进程,而高活性脐带间充质干细胞治疗树鼩脾脏衰老尚未见报道。目的:探讨高活性脐带间充质干细胞对树鼩脾脏衰老的干预作用及机制。方法:从剖腹产的新生树鼩脐带组织中分离、培养和获得高活性脐带间充质干细胞,三系分化试剂盒检测成脂、成骨、成软骨分化能力,流式细胞术检测细胞周期和表面标志物。以感染复数值分别为100,120,140,160,180,200的吉凯基因绿色荧光蛋白转染第2代高活性脐带间充质干细胞,筛选最佳转染条件;转染后的第4代高活性脐带间充质干细胞尾静脉输注给老年治疗组树鼩,青年对照组和老年模型组不予特殊处理,治疗4个月时取脾脏组织,苏木精-伊红染色观察脾脏组织结构;β-半乳糖苷酶染色检测衰老相关半乳糖苷酶活性;免疫组织化学染色检测p21和p53蛋白表达水平;Ki67和PCNA免疫荧光染色检测细胞增殖活性;免疫荧光染色检测脾脏自噬蛋白分子Beclin1和APG5L/AtG5表达水平;活性氧荧光染色检测脾脏组织的活性氧含量;CD3免疫荧光染色检测总t淋巴细胞比例变化;酶联免疫吸附实验检测脾脏组织白细胞介素1β和转化生长因子β1分泌水平;DAPI复染细胞核观察绿色荧光蛋白标记的高活性脐带间充质干细胞在脾脏组织的分布情况。结果与结论:①高活性脐带间充质干细胞呈核小短梭形、鱼群样生长,G_(0)/G_(1)期占比大,具有向成脂、成骨和成软骨分化潜能。②感染复数为140且转染72 h为吉凯基因绿色荧光蛋白标记树鼩高活性脐带间充质干细胞的最佳条件。③与老年模型组相比,老年治疗组树鼩脾脏组织细胞排列紧密,白髓面积增加(P0.05),脾脏组织衰老相关半乳糖苷酶活性水平降低(PtG5表达上调(Pt细胞比例增加(P0.05)。与青年对照组相比,以上检测指标在老年治疗组中均有显著差异(P<0.05)。④冰冻组织切片观察可见老年治疗组树鼩脾脏组织中有绿色荧光蛋白标记的绿色荧光细胞。结果表明:静脉输注高活性脐带间充质干细胞可迁移至脾脏组织,抑制活性氧产生,下调衰老相关蛋白分子表达,诱导细胞自噬,促进细胞增殖,降低慢性炎症,进而改善脾脏组织结构和功能。

关键词：高活性脐带间充质干细胞树鼩脾脏衰老免疫自噬炎症