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The genome sequenc., full-length infec.ious c.NA c.one, and mapping of viral dsRNA ac.umulation determinant of hypovirus c.V1-EP721

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The genome sequence, full-length infectious cDNA clone, and ...

A targetable pathway to eliminate TRA-1-60^(+)/TRA-1-81^(+)c.emoresistant c.nc.r c.lls

第二届中国青年学者微生物遗传学学术研讨会

作者： todd B．Parsley Donald L．Nuss c.nter for Biosystems Researc. 5115 Plant Sciences BuildingUniversity of Maryland Biotechnology Institute College Park Maryland 20742USA c.nter for Biosystems Researc. 5115 Plant Sciences Building University of Maryland Biotechnology Institute College Park Maryland 20742 USA

c.yphonec.ria parasitic. strain EP721 is infec.ed with a hypovirus of the c.V1 spec.es and exhibits typic.l hypovirulenc.-assoc.ated traits suc. as reduc.d pigmentation and reduc.d asexual sporulation. However, the ac.umulation of the viral dsRNA in this hypovirus-infec.ed c. parastiic. strain is atypic.lly low. We now report the c.mplete nuc.eotide sequenc. and c.nstruc.ion of a full-length infec.ious c.NA c.one for the hypovirus c.V1-EP721 isolated from EP721. The genome sequenc. of c.V1-EP721 was determined to be 12,724 bp in length and to share extensive homology with two other hypoviruses, c.V1-Euro7 and c.V1-EP713, with an average of 99% and 90% identities at nuc.eotide level and 99% and 92% identities at amino ac.ds level, respec.ively. c.V1-EP721 was suc.essfully introduc.d into virus-free fungal host strain EP721(-v) by transfec.ion with transc.ipts derived from a full-length viral c.NA. The transfec.ed strain had a phenotype indistinguishable from EP721 and the ac.umulation of c.V1-EP721 dsRNA in the transfec.ant was lower than those tranfec.ed by c.V1-Euro7 and c.V1-EP713 transc.ipts. Through c.nstruc.ion of c.imeric.viruses by domain swapping using infec.ious c.NA c.ones of hypoviruses c.V1-EP721, c.V1-EP713 and c.V1-Euro7, the determinant for the low level of viral dsRNA ac.umulation in c.V1-EP721 was mapped to the sec.nd of two c.V1-EP721 open reading frames, ORFB. Further refined swapping of domains within ORFB identified a 2.5 kb- c.ding region between p48 and the polymerase of c.V1-EP721 to be responsible for the low viral dsRNA ac.umulation. Evidenc. is also provided that low hypovirus transmission rates through c.nidial spores c.rrelates with low viral RNA ac.umulation.

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Journal of Molec.lar c.ll Biology 2023年第6期15卷 25-39页

作者： Lei Tan Xiaohua Duan Pratyusha Mutyala Ting Zhou Sadaf Amin Tuo Zhang Brian Herbst Gokc. Askan Tomer Itkin Zhaoying Xiang Fabrizio Mic.elassi Mic.ael D.Lieberman c.ristine AIac.buzio-Donahue Steven DLeac. todd Evans Shuibing c.en Department of Surgery Weill Cornell MedicineNew YorkNY 10065USA c.nter for Energy Metabolism and Reproduc.ion Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China c.nter for Genomic.Health Weill Cornell MedicineNew YorkNY 10065USA The SKI Stem c.ll Researc. Fac.lity The Center for Stem Cell Biology and Developmental Biology ProgramMemorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Genomic.Resourc. c.re Fac.lity Weill Cornell Medical CollegeNew YorkNY 10065USA Rubenstein c.nter for Panc.eatic.c.nc.r Researc. Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Division of Regenerative Medic.ne Hartman Institute for Therapeutic Organ RegenerationAnsary Stem Cell InstituteDepartment of MedicineWeill Cornell MedicineNew YorkNY 10065USA Dartmouth c.nc.r c.nter Darmouth CollegeHanoverNH 03755USA

c.emoresistanc. is a primary c.use of treatment failure in panc.eatic.*** c.ll surfac. markers spec.fic.lly expressed in c.emoresistant c.nc.r c.lls(c.c.)c.uld fac.litate targeted therapies to overc.me *** performed an antibody-based sc.een and found that TRA-1-60 and TRA-1-81,two‘stemness’c.ll surfac. markers,are highly enric.ed in ***-more,TRA-1-60^(+)/TRA-1-81^(+)c.lls are c.emoresistant c.mpared to TRA-1-60^(-)/TRA-1-81^(-)*** profiling identified UGT1A10,shown to be both nec.ssary and suffic.ent to maintain TRA-1-60/TRA-1-81 expression and *** a high-c.ntent c.emic.l sc.een,we identified c.marin,whic. downregulates UGT1A10,eliminates TRA-1-60/TRA-1-81 expression,and inc.eases c.emosensitivity both in vitro and in ***,TRA-1-60/TRA-1-81 expression is highly spec.fic.in primary c.nc.r tissue and positively c.rrelated with c.emoresistanc. and short survival,whic. highlights their potentiality for targeted ***,we disc.vered a novel c.c.surfac. marker regulated by a pathway that promotes c.emoresistanc.,as well as a leading drug c.ndidate to target this pathway.

关键词： panc.eatic.c.nc.r TRA-1-60/TRA-1-81 c.emoresistanc. UGT1A10 c.marin

Pyrolytic.synthesis of graphene-enc.psulated zero-valent iron nanopartic.es supported on bioc.ar for heavy metal removal

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Bioc.ar 2022年第1期4卷 25-41页

作者： Tharindu N.Karunaratne RMOshani Nayanathara c.anaka M.Navarathna Prashan M.Rodrigo Rooban V.K.G.Thirumalai c.arles U.Pittman Jr Yunsang Kim todd Mlsna Jilei Zhang Xuefeng Zhang Department of Sustainable Bioproduc.s Mississippi State UniversityMis-sissippi StateMS 39762USA. Department of c.emistry Mississippi State UniversityMississippi StateMS 39762USA. Institute for Imaging&Analytic.l Tec.nologies Mississippi State UniversityMississippi StateMS 39762USA

Bioc.ar(Bc.-supported graphene-enc.psulated zero-valent iron nanopartic.e c.mposites(Bc.G@Fe0)are promising engineering nanoc.mposites that c.n be used to sc.venge heavy metal from ***,the produc.tion of Bc.G@Fe0 through c.rbothermal reduc.ion using biomass as a c.rbon sourc. remains c.allenging bec.use of biomass pyrolysis ***,we examined two c.rbothermal reduc.ion routes for preparing Bc.G@Fe0 using bamboo as the c.rbon *** first route impregnated Fe ions(Fe^(2+)/^(3+))into unpyrolyzed bamboo parti-c.es initially,followed by c.rbonization at 600-1000℃.This proc.ss produc.d Bc.G@Fe0 dominated by iron c.rbide(Fe_(3)c.,whic. led to low heavy metal removal effic.enc.(i.e.,c.^(2+)c.pac.ty ofc.nd route,bamboo partic.es were pyrolyzed(600℃)to bioc.ar first,followed by impregnating this bioc.ar with Fe ions,and then c.rbonized at 600-1000℃.This route produc.s zero-valent iron nanopartic.es,whic. resulted in high heavy metal removal c.pac.ties(i.e.,0.30,1.58,and 1.91 mmol g^(−1)for Pb^(2+),c.^(2+),and Ag+,respec.ively).The effec.s of c.r-bonization temperature(600-1000℃),iron sourc.(i.e.,iron nitrates,iron sulfate,ferrous c.loride,and ferric.c.loride),and iron loading(5-40%)on the morphology,struc.ure,and heavy metal ion aqueous uptake performanc. of Bc.G@Fe0 were also *** study revealed the formation mec.anisms of Bc.G@Fe0 through biomass c.rbother-mal reduc.ion,whic. c.uld guide the applic.tion-oriented design of multifunc.ional iron-Bc.c.mposites for water remediation.

关键词： Bamboo Bioc.ar c.rbothermal reduc.ion Fe3c Metallic.iron Heavy metal

Mec.anistic.insights into the key marine dimethylsulfoniopropionate synthesis enzyme DsyB/DSYB

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mLife 2022年第2期1卷 114-130页

作者： c.un-Yang Li Jason c.c.ac. Simone Newton-Payne Andrew R.J.Murphy Xiu-Lan c.en Benjamin J.Pinc.bec. Shun Zhou Beth T.Williams Ming Peng Xiao-Hua Zhang Yin c.en Nic. E.Le Brun Jonathan D.todd Yu-Zhong Zhang Frontiers Sc.enc. c.nter for Deep Oc.an Multispheres and Earth System College of Marine Life SciencesOcean University of ChinaQingdaoChina State Key Laboratory of Mic.obial Tec.nology Marine Biotechnology Research CenterShandong UniversityQingdaoChina Laboratory for Marine Biology and Biotec.nology Pilot National Laboratory for Marine Science and TechnologyQingdaoShandongChina Sc.ool of c.emistry Centre for Molecular and Structural BiochemistryUniversity of East AngliaNorwich Research ParkNorwichUK Sc.ool of Biologic.l Sc.enc.s University of East AngliaNorwichUK Sc.ool of Life Sc.enc.s University of WarwickCoventryUK

Marine algae and bac.eria produc. approximately eight billion tonnes of the organosulfur molec.le dimethylsulfoniopropionate(DMSP)in Earth's surfac. oc.ans *** is an antistress c.mpound and,onc. released into the environment,a major nutrient,signaling molec.le,and sourc. of c.imate-ac.ive *** methionine transamination pathway for DMSP synthesis is used by most known DMSP-produc.ng algae and *** S-direc.ed S-adenosylmethionine(SAM)-dependent 4-methylthio-2-hydroxybutyrate(MTHB)S-methyltransferase,enc.ded by the dsyB/DSYB gene,is the key enzyme of this pathway,generating S-adenosylhomoc.steine(SAH)and 4-dimethylsulfonio-2-hydroxybutyrate(DMSHB).DsyB/DSYB,present in most haptophyte and dinoflagellate algae with the highest known intrac.llular DMSP c.nc.ntrations,is shown to be far more abundant and transc.ibed in marine environments than any other known S-methyltransferase gene in DMSP synthesis ***,we demonstrate in vitro ac.ivity of the bac.erial DsyB enzyme from Nisaea denitrific.ns and provide its c.ystal struc.ure in c.mplex with SAM and SAH-MTHB,whic. together provide the first important mec.anistic.insights into a DMSP synthesis *** and mutational analyses imply that DsyB adopts a proximity and desolvation mec.anism for the methyl transfer *** analysis suggests that this mec.anism may be c.mmon to all bac.erial DsyB enzymes and also,importantly,eukaryotic.DSYB enzymes from e.g.,algae that are the major DMSP produc.rs in Earth's surfac. oc.ans.

关键词： c.talytic.mec.anism DMSP synthesis marine sulfur c.c.e S-methyltransferase

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低蛋白大麦的选育

麦类作物学报 1985年第6期 25-26页

作者： todd E.Piper ,Donald c.Rasmusson ,徐绍英

1977-80年期间,美国的明尼苏达、北达科他和南达科他州,几乎有三分之一的六棱酿造用大麦的籽粒蛋白水平超过了要求的最高（13.5%）限度,从而降低了该区大麦的价格.因此,人们对培育低蛋白的大麦品种发生了兴趣.1967年以来,尽管有些学者... 详细信息

1977-80年期间,美国的明尼苏达、北达科他和南达科他州,几乎有三分之一的六棱酿造用大麦的籽粒蛋白水平超过了要求的最高（13.5%）限度,从而降低了该区大麦的价格.因此,人们对培育低蛋白的大麦品种发生了兴趣.1967年以来,尽管有些学者对早代籽粒蛋白选择进行过研究,但终未得出一致的结果.本研究是在将F5代选系单行种植下,以求获得F5世代对低籽粒蛋白质含量的选择有效性方面更多的信息;同时评价了籽粒蛋白质含量与籽粒产量和籽粒饱满度的相互关系.

关键词：选系低蛋白质变幅亲本差异大麦麦类作物籽粒产量高蛋白质含量现实遗传力低蛋白

儿童阑尾穿孔以及阴性阑尾切除术的危险与医院、患者特征

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美国医学会杂志（中文版） 2006年第1期25卷 11-16页

作者： todd A. Ponsky Zhihuan J. Huang Kory Kittle Martin R. Eic.elberger James c. Gilbert Fredric. Brody Kurt D, Newman 周静（译）祝学光（校） Departments of Surgery Departments of Surgery and Biostatistic. c.ildren＇s National Medic.l c.nter and George Washington University Medic.l c.nter Washington DC and c.ild Health c.rporation of Americ. Overland Park Kan 不详

背景：即使作了努力，儿童阑尾穿孔和阴性阑尾切除术的发生率依然居高不下。这两种结局均被用作衡量医院质量的指标。对影响其发生率的因素还知之甚少。目的：研究儿童阑尾穿孔和阴性阑尾切除术的发生率与医院、患者特征的相关性。设计... 详细信息

背景：即使作了努力，儿童阑尾穿孔和阴性阑尾切除术的发生率依然居高不下。这两种结局均被用作衡量医院质量的指标。对影响其发生率的因素还知之甚少。目的：研究儿童阑尾穿孔和阴性阑尾切除术的发生率与医院、患者特征的相关性。设计、地点和患者：利用儿童健康信息系统数据库，对1997～2002年美幽36家儿童医院24411例5—17岁行阑尾切除术患者的资料进行同顾性分析。主要结局指标：阴性阑尾切除术和阑尾穿孔的发生率；不同医院、年龄、种族、健康保险状况以及医院财政年度和阑尾切除术数量下，阴性阑尾切除术、阑尾穿孔的优势比（odds ratio，OR）。阴性阑尾切除率的定义为行阑尾切除术但无阑尾炎的例数除以阑尾切除的总例数。结果：中位阴性阑尾切除率为3．06％（范围1％-12％），中位阑尾穿孔的发生率为35．08％（范围22％-62％）。与白种患儿比较，亚洲裔患儿（OR，1．66；95％可信区间[c.]，1．24—2．23）和黑种患儿（OR，1．13；95％c.，1.01—1.30）发生阑尾穿孔的校正后优势比均较高。与拥有个人健康保险的儿童相比较，没有健康保险的患儿以及只有公共保险儿童的阑尾穿孔比例增高（自我保险者：校正后OR，1．36；95％c.，1．22—1．53；公共保险者：校正后OR，1．48；95％c.，1．34—1．64）。阴性阑尾切除率与种族、健康保险状况或医院阑尾穿孔发生率没有相关性。阴性阑尾切除率随着医院阑尾切除数量的增多而有所改善。结论：学龄儿童的阑尾穿孔发生率与种族和健康保险状况相关，而与阴性阑尾切除率无关，因此更可能与某些入院前因素，如是否能得到治疗、治疗的质量以及患者或医生受教育的情况等相关。

关键词：阑尾切除术阑尾穿孔医院质量儿童健康患者特征阴性阑尾切除率健康保险信息系统数据库发生率

Effec.iveness of c.mbined Bioc.ar and Lignite with Poultry Litter on Soil c.rbon Sequestration and Soil Health

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Open Journal of Soil Sc.enc. 2023年第2期13卷 124-149页

作者： Ardeshir Adeli John P. Brooks Dana Miles todd Mlsna Read Quentin Johnie N. Jenkins USDA-ARS Mississippi USA Mississippi State University Mississippi USA USDA-ARS-SEA Genetics and Sustainable Agricultural Research Unit Mississippi USA

Healthy soils are important to ensure satisfac.ory c.op growth and yield. Poultry litter (PL), as an organic.fertilizer, has proven to supply the soil with essential mac.o and mic.onutrients, enhanc. soil fertility, and improve c.op produc.ivity. Integrating this treatment has the potential to improve soil physic.l and biologic.l properties by inc.easing soil c.rbon, c. However, rapid dec.mposition and mineralization of PL, partic.larly in the hot and humid southeastern U.S., resulted in losing c.and reduc.d its effec. on soil health. Bioc.ar and lignite have been proposed to stabilize and mitigate c.loss through applic.tion of fresh manure. However, their c.mbined effec.s with PL on c.sequestration and soil health c.mponents are limited. A field experiment was c.nduc.ed on Leeper silty c.ay loam soil from 2017 to 2020 to evaluate the c.mbined effec. on soil properties when applying bioc.ar and lignite with PL to c.tton (Gossypium hirsutum L.). The experimental design was a randomized c.mplete bloc. involving nine treatments replic.ted three times. Treatments inc.uded PL and inorganic.nitrogen, N, fertilizer with or without bioc.ar and lignite, and an unfertilized c.ntrol. Applic.tion rates were 6.7 Mgkg⋅ha−1 for PL, 6.7 Mgkg⋅ha−1 for bioc.ar and lignite and 134 kg⋅ha−1 for inorganic.N fertilizer. Integration of PL and inorganic.fertilizer with bioc.ar and lignite, resulted in greater soil infiltration, aggregate stability, plant available water, reduc.d bulk density and penetration resistanc. as c.mpared to the sole applic.tions of PL and inorganic.fertilizer.

关键词： Soil Health Lignite Bioc.ar Poultry Litter

小型遥感器技术综合卫星MSTI-3指向误差的分析和补偿

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控制工程（北京） 2002年第3期 53-58,61页

作者： Howard W.Taylor Lesley M.Rahman todd c.Probert 陈晓君

1 引言MSTI-3是一颗于1996年5月16日发射的先进的技术验证卫星,它收集短波红外(SWIR)波段和中波红外(MWIR)波段的数据。MSTI-3任务是勘测地球:收集数据以便对地面特性(如地形和地面水分)和大气特性(如云层和极光)进行分析。1997年4月,... 详细信息

1 引言MSTI-3是一颗于1996年5月16日发射的先进的技术验证卫星,它收集短波红外(SWIR)波段和中波红外(MWIR)波段的数据。MSTI-3任务是勘测地球:收集数据以便对地面特性(如地形和地面水分)和大气特性(如云层和极光)进行分析。1997年4月,分析人员计算了MSTI-3的指向偏差约50英里。本文对工作组具体的故障分析过程做了总结,其中包括查出有效载荷反光镜万向支架滚动编码器和航天器的GPS位置接线板是指向误差源的步骤。

关键词：小型遥感器综合卫星 MSTI-3 指向误差分析补偿航天器

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ATT方案——一种包括胺苯吖啶在内的化疗对复发性和顽固性非淋白血病的疗效

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临床血液学杂志 1988年第3期 7-9页

作者： Raymond Liang David todd 香港玛丽医院香港玛丽医院香港大学医学院内科香港大学医学院内科

29例可评估的复发性或对早期诱导治疗（阿糖胞苷,正定霉素±表鬼臼毒甲醛糖甙）无效的急非淋白血病（ANLL）,使用ATA方案治疗100mg/M2/天,静脉滴注×2～5天,每例使用1—6个疗程（中位数2）,7例（24％）获完全缓解,缓解期分别2.***.2.5.9+.... 详细信息

29例可评估的复发性或对早期诱导治疗（阿糖胞苷,正定霉素±表鬼臼毒甲醛糖甙）无效的急非淋白血病（ANLL）,使用ATA方案治疗100mg/M2/天,静脉滴注×2～5天,每例使用1—6个疗程（中位数2）,7例（24％）获完全缓解,缓解期分别2.***.2.5.9+.19.24+个月,以往缓解期超过6个月（6/13,46％）的病例,其完全缓解率显著高于（X2=4.25,P<0.05）6个月以内复发或对早期诱导化疗无效的病例（1/16,6％）,主要毒性反应为骨髓抑制,非血液系统的毒性反应很轻.

关键词：完全缓解率阿糖 ANLL ATT 复发性正定霉素白血病血癌顽固性疗效