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未来食品学报(英文) 2024年第3期4卷 233-240页

作者： Lingxin Geng Jiaojiao Han Jun Zhou Ye Li tinghong ming Zhen Zhang Chenyang Lu Xiurong Su State Key Laboratory for Quality and Safety of Argo-products School of Marine ScienceNingbo UniversityNingbo 315211China

Diabetic nephropathy(DN)is a common type of end-stage renal disease and glomerular mesangial cells(GMCs)are widely used as a cell model for DN.This study firstly investigated the inhibitory effects of the Apostichopus japonicus and Acaudina leucoprocta hydrolysates on cellular growth under high-glucose treatment,better inhibitory effect of A.japonicus hydrolysate was observed compared to that of A.leucoprocta hydrolysate.Subsequently,the global transcription profiles obtained via microarray analysis showed that 6070 and 7015 genes were identified in the A.japonicus and A.leucoprocta groups compared with the model group,respectively.Among them,transcriptions of the slc30a4,slc35dl,tppp3,tp53inpl,bcl-2,apafl,alox12b and adrala genes were restored from the levels of the model group to those of the control group,contributed to cell mitosis and proliferation in both treatment groups.In addition,other apoptosis-related genes,such as bcl-6,clu,foxo3 and akt,showed opposite trends between two groups,which might cause the difference in inhibitory effect.We preliminarily proposed that the regulation effects of A.japonicus and A.leucoprocta on the genes involved in cellular mitosis,proliferation and apoptosis,might contribute to their inhibitory activity on GMCs under high-glucose environment.

关键词： Diabetic nephropathy Apostichopus japonicus Acaudina leucoprocta Hydrolysate Rat mesangial cell Microarray

Tuna dark muscle peptides modulate host iron metabolism in the intestine through the gut microbiota and ameliorate iron deficiency anemia

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Food Science and Human Wellness 2024年

作者： Jiaojiao Han Jiayi Xiong Zhixiang Bai Yang Qin Chenyang Lu Jun Zhou Ye Li tinghong ming Zhen Zhang Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo University School of Marine Science Ningbo University

Iron deficiency anemia (IDA) is a nutritional deficiency disease with a high incidence rate worldwide. Bioactive peptides are safe and effective， have multiple functions and can serve as potential candidates for alleviating IDA. In this study， the anti-anemia effects of tuna dark muscle peptides were explored in a diet-induced IDA mouse model. The results showed that tuna dark muscle peptides alleviated the IDA phenotype， oxidative stress and iron metabolism. In addition， tuna dark muscle peptides reversed gut microbiota dysbiosis in IDA mice. Furthermore， the transplanted fecal microbiota from tuna dark muscle peptide-treated mice also alleviated IDA symptoms and regulated iron metabolism and the gut microbiota， indicating that the anti-anemic effects were at least partially mediated by the gut microbiota. Thus， we identified a new and safe prebiotic material to alleviate IDA and provided ideas for the development of peptides. At the same time， these data also provided a theoretical basis for fecal microbiota transplantation (FMT) to alleviate IDA.

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Modulation of gut microbiota and serum metabolome by Apostichopus japonicus derived oligopeptide in high-fructose diet-induced hyperuricemia in mice

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Food Science and Human Wellness 2024年

作者： Jun Zhou Ziyan Wang Zhixuan Zhang Jiaojiao Han Ying Feng Jing Zhang Zhen Zhang Ye Li tinghong ming Chenyang Lu Rixin Wang Xiurong Su School of Marine Science Ningbo University Faculty of Food Science Zhejiang Pharmaceutical College Key Laboratory of Aquacultural Biotechnology Ministry of Education Ningbo University College of Life Sciences Tonghua Normal University State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo University

Fructose consumption has risen dramatically in recent decades due to the use of sucrose and high fructose corn syrup in beverages and processed foods， contributing to rising rates of hyperuricemia. The purpose of this experiment was to explore the anti-hyperuricemia effects of an active oligopeptide (GPSGRP) derived from sea cucumber in fructose induced hyperuricemia mouse model， and to clarify the underlying mechanism in sight of gut microbiota and serum metabolites. Peptide GPSGRP treatment rebalanced uric acid metabolism and alleviated inflammatory response in mice. In addition， treatment with GPSGRP decreased the abundance of Bacteroides and Proteobacteria at the phylum level， Muribaculum， Prevotella and Bacteroides at the genus level， and inhibited the related pathways of purine metabolism and glycolysis/gluconeogenesis metabolism. Moreover， serum metabolites， including linoleic acid， indole and its derivatives， arachidonic acid and uridine， as well as related metabolic pathways， such as tricarboxylic acid cycle， ketone production and sugar production， were altered in response to GPSGRP treatment. This study provides a valuable reference for the application and development of marine biological peptides in uric acid management.

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植物乳杆菌发酵草鱼肉挥发性成分的变化规律

食品科学 2015年第20期36卷 174-180页

作者：裘迪红欧昌荣苏秀榕明庭红浙江医学高等专科学校浙江杭州310053 宁波大学海洋学院浙江宁波315211

研究植物乳杆菌发酵草鱼的过程中挥发性成分变化规律。利用电子鼻、顶空固相微萃取和气相色谱-质谱联用技术对草鱼发酵不同时间段的挥发性成分进行分析。结果表明,电子鼻能够较好地区分发酵不同阶段的鱼肉样品的风味,各阶段的成分有显... 详细信息

研究植物乳杆菌发酵草鱼的过程中挥发性成分变化规律。利用电子鼻、顶空固相微萃取和气相色谱-质谱联用技术对草鱼发酵不同时间段的挥发性成分进行分析。结果表明,电子鼻能够较好地区分发酵不同阶段的鱼肉样品的风味,各阶段的成分有显著差异,进一步采用气相色谱-质谱法对不同时间的草鱼发酵样品中的挥发性成分进行分析,鉴定出54种挥发性物质,在发酵过程中,土腥味的己醛、庚醛等醛类物质逐渐减少;蘑菇香味的1-辛烯-3-醇含量逐渐增多,发酵后期产生了有薄荷香味的丙酮,以及有奶油香味的乙偶姻等增香成分,这和感官评定的结果一致。

关键词：植物乳杆菌草鱼肉电子鼻气相色谱-质谱挥发性成分

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水产养殖专业遗传学实验教学改革

高师理科学刊 2023年第9期43卷 95-98页

作者：张真冉照收沈锡权明庭红吕志猛宁波大学海洋学院浙江宁波315211

遗传学是水产养殖专业的重要基础课程之一,其实验教学是帮助学生巩固理论知识,掌握实验技术的重要途径.以宁波大学水产养殖专业遗传学实验教学为例,针对目前教学内容安排和考核方式的弊端,提出一系列完善措施,如引入以斑马鱼为载体的实... 详细信息

遗传学是水产养殖专业的重要基础课程之一,其实验教学是帮助学生巩固理论知识,掌握实验技术的重要途径.以宁波大学水产养殖专业遗传学实验教学为例,针对目前教学内容安排和考核方式的弊端,提出一系列完善措施,如引入以斑马鱼为载体的实验教学内容,增加学生参与课前材料准备为依据的评价体系等.以期促进水产类学生学习与操作遗传学实验课程的参与度,提高学习效果,为水产类课程建设中的学生培养模式及实践课环节提供参考.

关键词：水产养殖遗传学实验斑马鱼考核方式

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基于植物乳杆菌发酵草鱼脱腥增香的研究

中国食品学报 2017年第10期17卷 202-210页

作者：明庭红裘迪红周君李晔张春丹张迪骏苏秀榕宁波大学海洋学院浙江宁波315211 杭州医学院杭州310053

目的:研究植物乳杆菌发酵草鱼的脱腥增香效果。方法:利用发酵鱼肉气味的响应值及载荷分析优化电子鼻传感器阵列,对优化后的电子鼻检测数据进行主成分分析(PCA),并采用顶空固相微萃取与气-质谱联用仪(HS-SPME-GC-MS)对植物乳杆菌发酵草... 详细信息

目的:研究植物乳杆菌发酵草鱼的脱腥增香效果。方法:利用发酵鱼肉气味的响应值及载荷分析优化电子鼻传感器阵列,对优化后的电子鼻检测数据进行主成分分析(PCA),并采用顶空固相微萃取与气-质谱联用仪(HS-SPME-GC-MS)对植物乳杆菌发酵草鱼不同时间段挥发性风味成分进行分析和鉴定,分析发酵过程中腥味物质和香味成分的变化情况。结果:电子鼻能灵敏检测到草鱼在发酵过程中气味的变化,主成分分析显示各样品间差异明显。采用GC-MS在0,24,48,72,96,120,144,168 h分别检测出38,40,47,51,52,43,47,44种成分,主要为烃类、醛类、醇类、酮类、酯类、酸类和胺类及芳香类化合物;发酵过程中生成相对含量较高的类似柠檬香的D-柠檬烯和类似蘑菇香的1-辛烯-3-醇以及类似脂肪香的3-羟基-2-丁酮等,而草鱼肉中的主要腥味物壬醛和2,4-癸二烯醛等逐渐减少。结论:在发酵120 h后植物乳杆菌发酵草鱼的脱腥增香效果显著。

关键词：草鱼植物乳杆菌电子鼻顶空固相微萃取气-质联用脱腥增香

基于高通量测序技术对浙江传统发酵蔬菜微生物多样性的解析

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食品科学 2021年第6期42卷 178-185页

作者：鲍伟韩姣姣张旨轩芦晨阳周君明庭红李晔苏秀榕宁波大学农产品质量安全危害因子与风险防控国家重点实验室浙江宁波315211 宁波大学海洋学院浙江宁波315832 宁波大学食品与药学学院浙江宁波315832

以浙江地区传统发酵蔬菜臭冬瓜、臭苋菜梗、酸笋和酸茭白为研究对象,采用Illunina MiSeq高通量测序技术,分别分析浙江传统发酵蔬菜的汁液、固形物和培养物中细菌和真菌微生物群落组成和多样性,探讨“臭”和“酸”类发酵食品的微生物群... 详细信息

以浙江地区传统发酵蔬菜臭冬瓜、臭苋菜梗、酸笋和酸茭白为研究对象,采用Illunina MiSeq高通量测序技术,分别分析浙江传统发酵蔬菜的汁液、固形物和培养物中细菌和真菌微生物群落组成和多样性,探讨“臭”和“酸”类发酵食品的微生物群落组成差异。结果表明:14个样品中细菌和真菌的测序覆盖率分别为0.97和0.99。分析细菌,臭苋菜梗固形物的可操作分类单元(operational taxonomic unit,OTU)最多、Shannon指数最高;而酸笋固形物中的OTU最少,酸笋汁的Shannon指数最低;且臭苋菜梗汁、固形物和培养物以及酸笋固形物和培养物的菌群结构更为接近。分析真菌,臭冬瓜培养物和酸茭白固形物中的真菌OTU最多,臭苋菜梗培养物中的OTU最少,而酸茭白汁中没有检测出真菌;酸笋汁的Shannon指数最高,臭苋菜梗培养物的Shannon指数最低;除卤水、臭苋菜梗汁和酸笋样品外其余样品的真菌群落组成更为接近。在属水平,臭冬瓜培养物、酸笋汁、酸笋固形物和酸茭白汁的优势细菌属分别为脱硫弧菌属(Desulfovibrio)、克雷伯氏菌属(Klebsiella)、埃希菌属(Escherichia)和Caproiciproducens,卤水培养物、酸茭白固形物和培养物的优势细菌属为梭菌属(Clostridium),臭苋菜梗汁、固形物和培养物的优势细菌属为拟杆菌属(Bacteroides),卤水、臭冬瓜汁和固形物的优势细菌属为乳酸杆菌属(Lactobacillus);卤水的优势真菌属为柯达酵母属(Kodamaea),臭苋菜梗汁、固形物和培养物的优势真菌属为假丝酵母属(Candida),而其他样品的优势真菌属为Leptospora。

关键词：传统发酵蔬菜酸笋和酸茭白 16S rDNA高通量测序微生物多样性

酒瓶挂珠和材质分析及其对白酒中钠浓度的影响分析

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酿酒科技 2024年第5期 59-64页

作者：王明王辅王星月廖源杨琴张廷红泸州老窖股份有限公司四川泸州646000 西南科技大学材料与化学学院四川绵阳621010

本研究分析了挂珠和不同材质酒瓶的玻璃成分、表面和接触角,在此基础上,用其贮存不同档次的白酒,对货架期的白酒定期进行钠(Na)浓度检测,分析其变化规律。结果表明:选取的酒瓶玻璃主要成分SiO_(2)含量为82~83mol%,密度为2.50~2.52g/cm^(... 详细信息

本研究分析了挂珠和不同材质酒瓶的玻璃成分、表面和接触角,在此基础上,用其贮存不同档次的白酒,对货架期的白酒定期进行钠(Na)浓度检测,分析其变化规律。结果表明:选取的酒瓶玻璃主要成分SiO_(2)含量为82~83mol%,密度为2.50~2.52g/cm^(3),质地均匀;存储过程中,挂珠现象对白酒中Na浓度变化几乎无影响;初探表明接触角大的酒瓶长时间存储白酒可能会引起白酒中Na浓度增加。研究结果可为相关企业把控产品质量和白酒储藏提供重要参考。

关键词：玻璃酒瓶材质挂珠成分分析白酒贮存

金枪鱼鱼白的植物乳杆菌发酵脱腥增香作用研究

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中国食品学报 2019年第2期19卷 147-154页

作者：黄忠白明庭红董丽莎韩姣姣孙婷婷何珊张迪骏黄健周君李晔苏秀榕宁波大学海洋学院浙江宁波315211 北京普析通用仪器有限责任公司北京101200

目的:利用植物乳杆菌发酵金枪鱼鱼白来改善其风味,研究脱腥增香的效果,为水产品加工提供参考。方法:利用主成分分析(PCA)和特征风味雷达图来确定新鲜鱼白和发酵不同时间的鱼白及其发酵液的挥发性物质变化趋势,采用顶空固相微萃取与气-... 详细信息

目的:利用植物乳杆菌发酵金枪鱼鱼白来改善其风味,研究脱腥增香的效果,为水产品加工提供参考。方法:利用主成分分析(PCA)和特征风味雷达图来确定新鲜鱼白和发酵不同时间的鱼白及其发酵液的挥发性物质变化趋势,采用顶空固相微萃取与气-质联用仪(HS-SPME-GC-MS)对这个过程中的挥发性风味成分进行分析,结合感觉阈值,相对气味活度值(ROAV)确定发酵鱼白的主体风味成分。结果:PCA和雷达图显示新鲜鱼白与发酵鱼白的气味差异明显,发酵液的气味变化相对较小,较稳定。通过GC-MS在新鲜鱼白、种子发酵液、发酵12 h、24 h、36 h和48 h的鱼白及其发酵液中分别检测出22,13,12,23,35,34,11,13,15,17种挥发性物质。经过发酵的鱼白中庚醛、己醛、1-辛烯-3-醇等具有腥味的物质减少,具有奶香气味的己酸、2,3-丁二酮、乙偶姻等物质含量增加。这些物质是发酵鱼白中的主体风味物质。结论:植物乳杆菌发酵鱼白24 h后,其风味得到改善,脱腥增香的效果非常明显。

关键词：金枪鱼鱼白植物乳杆菌固相微萃取气-质谱联用仪相对气味活度值

Transcriptome analysis reveals tumor antigen and immune subtypes of melanoma

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Oncology Research 2023年第3期31卷 389-403页

作者： JIAHENG XIE MENGMENG OU PAN YU DAN WU QIKAI TANG YUAN CAO JING HANG LU YIN tinghong XIANG ming WANG JINGPING SHI Department of Burn and Plastic Surgery The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China Department of Rheumatology and Immunology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008China Department of Neurosurgery The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China Fourth School of Clinical Medicine Nanjing Medical UniversityNanjing210029China Department of Ultrasound in Medicine The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China

Purpose:To screen potential tumor antigens for melanoma vaccine development and identify different immune subtypes.Methods:Transcriptional data(HTSEQ-FPKM)and clinical information of a 472 Melanoma cohort GDC TCGA Melanoma(SKCM)were downloaded from the UCSC XENA website(http://xena.***.edu/).Subsequently,transcriptome data and clinical information of 210 melanoma cohort GSE65904 were downloaded from Gene Expression Omnibus(GEO),a large global public database.All the transcriptome expression data matrices were log2 transformed for subsequent analysis.GEPIA,TIMER,and IMMPORT databases are also used for analysis.Cell function experiments were performed to validate the role of the IDO1 gene in melanoma cell line A375.Results:Our study provides potential tumor antigens for vaccine development in melanoma patients:GZMB,GBP4,CD79A,APOBEC3F,IDO1,JCHAIN,LAG3,PLA2G2D,XCL2.In addition,we divide melanoma patients into two immune subtypes that have significant differences in tumor immunity and may have different responses to vaccination.In view of the unclear role of IDO1 in melanoma,we selected IDO1 for cell assay validation.Cell function assay showed that IDO1 was significantly overexpressed in the melanoma A375 cell line.After IDO1 knockdown,the activity,invasion,migration and healing ability of A375 cell lines were significantly decreased.Conclusion:Our study could provide a reference for the development of vaccines for melanoma patients.

关键词： Melanoma mRNA vaccine Immune microenvironment Immunotherapy Bioinformatics analysis