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Drought and Grazing Effects on Oklahoma Phlox (Polemoniaceae, <i>Phlox oklahomensis</i>)

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American journal of Plant Sciences 2013年第6期4卷 9-13页

作者： tim L. Springer Stacey A. Gunter Ronald j. Tyrl Paul F. Nighswonger Department of Biology Northwestern Oklahoma State University Alva USA. Department of Botany Oklahoma State University Stillwater USA United States Department of Agriculture Agricultural Research Service Southern Plains Range Research Station Woodward Oklahoma USA

Oklahoma phlox (Phlox oklahomensis Wherry) is endemic to Butler, Chautauqua, Comanche, Cowley, and Elk Counties of Kansas and Woods and Woodward Counties of Oklahoma. The species comprises populations of a few scattered individuals to several hundred in mixed-grass prairie sites in Oklahoma where cow-calf production is the common agricultural enterprise. It has successfully withstood periods of short-term drought (1 to 4 years) under light to moderate continuous stocking rates (41 to 52 animal unit days per hectare). Under heavy continuous stocking rates and/or prolonged drought, populations of P. oklahomensis tend to decrease in size and number and may disappear in some localities. Prolonged heavy stocking rates and drought will disrupt the reproductive cycle, severely limiting seed production and recruitment of new individuals to populations. During drought periods, livestock managers should use lighter stocking rates or deferred grazing to ensure rapid recovery of all prairie plants, including P. oklahomensis, once the drought ends. This recommendation would likely sustain prairie sites during long-term (longer than 10 years) droughts as well.

关键词： Phlox oklahomensis Drought Grazing

Shifting Private timberland Ownership in South Carolina: Implications for Management Intensity

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Open journal of Forestry 2012年第4期2卷 279-285页

作者： john E. Hatcher jr. Thomas j. Straka Richard A. Harper tim O. Adams Forest Inventory and Analysis USDA Forest Service Southern Research Station Clemson USA School of Agricultural Forest and Environmental Sciences Clemson University Clemson USA South Carolina Forestry Commission Columbia USA School of Agricultural Forest and Environmental Sciences Clemson University Clemson USA

Beginning in the late 1970’s forest industry timberland gained the eye of financial investors. Diamond International and Crown Zellerbach were early firms that were purchased for the “break-up value” of their timberland. timberland was perceived as undervalued by investors and made forest industry firms attractive takeover targets. This started a process where forest industry divested of its timberland. Some firms formed separate entities for its timberland base. Acquisitions and mergers became popular in the industry. Some forest industry companies converted to real estate investment trusts, for tax and defensive reasons. Large institutional investors became interested in timberland as means to diversify their portfolios and increase financial performance. timber management investment organizations developed to manage and procure timberland for these institutional investors. Today little of the forest industry timberland remains with vertically-integrated forest products companies. South Carolina’s forest industry timberland decreased by about 800,000 ha since 1993 (or nearly 90%). This has implications for the state’s timber supply. Forest industry timberlands were some of the most productive and intensively managed forests in the state. We address how forest management might change on this timberland and how long-term timber supply might be impacted in the state.

关键词： Industrial timberland timber Investment Management Organization Real Estate Investment Trust timber Supply Forestry Investment

NetWare网连接Internet/Intranet的捷径——Novell IntranetWare IPX/IP网关介绍

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今日电子 1997年第3期 59-63,66页

作者： Henry j.Sprafkin tim Huntley

当前,与Intenert相连的网络数量的增长速度十分迅猛。预计,在专用局域网或“内部网”中,In-ternet技术的应用增长规模会更大。据估计,三分之二的现有LAN正在使用Novell NetWare,而且有越来越多的LAN将与Internet或企业内部网相连接。

关键词： Internet网 Intranet网网关网连接

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控制贮藏马铃薯损失的农药使用现状

农药译丛 1986年第3期 45-48页

作者： tim j.Dent 张志英国马铃薯销售部实验站

在英国,因细菌性软腐病(Erwinia carotavora ***)造成马铃薯贮藏期的损失很大,目前还没有发现在商业性贮藏条件下能有效防治该病害的化学药剂。因此现在用于贮藏马铃薯控制其收获后损失所使用的药剂通常是抑芽剂(如四氯硝基苯、氯苯胺... 详细信息

在英国,因细菌性软腐病(Erwinia carotavora ***)造成马铃薯贮藏期的损失很大,目前还没有发现在商业性贮藏条件下能有效防治该病害的化学药剂。因此现在用于贮藏马铃薯控制其收获后损失所使用的药剂通常是抑芽剂(如四氯硝基苯、氯苯胺灵控制因发芽造成的损失)、杀真菌剂(如噻菌灵、四氯硝基苯、仲丁胺控制真菌病害造成的损失)。

关键词：马铃薯混合粉剂药剂处理土豆茄属块茎地下茎四氯硝基苯四氯硝苯

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肝癌组织中HBV核心基因启动子突变的研究

中国病毒学 2000年第3期15卷 243-248页

作者：方钟燎杨进业王学燕庄辉 Roger Ling tim j Harrison 广西壮族自治区卫生防疫站南宁市530021 北京医科大学北京100083 Royal Free Hospital School of Medicine University of LondonRowl and HillSt.Lond on NW32PFUK

为深入了解乙肝病毒 (HBV)致癌机理 ,用套式PCR对广西 14例血清HBVDNA阳性的原发性肝癌患者癌组织、10例乙型病毒性肝炎患者血清及 10例乙肝病毒无症状携带者血清HBV核心基因启动子进行扩增 ,阳性者用Sanger氏双脱氧法做序列分析 ,发现... 详细信息

为深入了解乙肝病毒 (HBV)致癌机理 ,用套式PCR对广西 14例血清HBVDNA阳性的原发性肝癌患者癌组织、10例乙型病毒性肝炎患者血清及 10例乙肝病毒无症状携带者血清HBV核心基因启动子进行扩增 ,阳性者用Sanger氏双脱氧法做序列分析 ,发现肝癌组织 10例PCR阳性 ,阳性率 71.4 % ,所有PCR阳性标本的整合体均有乙肝病毒核心基因启动子双突变序列 (nt 176 2A→T ,176 4G→A) ,并且在其周围各序列都有不同部位的点突变 ,标本C14核苷酸的缺失突变高达10个。乙肝患者 6例PCR阳性 ,其中 3例乙肝病毒核心基因启动子发生双突变。无症状携带者中 4例PCR阳性 ,其中仅 1例发生双突变。结果提示乙肝病毒核心基因启动子双突变在肝癌患者中较常见 ,肝炎患者次之 ,无症状携带者居最后。

关键词：乙肝病毒核心基因启动子突变原发性肝癌

Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation

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Nano Research 2020年第2期13卷 485-495页

作者： jing Liu tim Hebbrecht Toon Brans Eef Parthoens Saskia Lippens Chengnan Li Herlinde De Keersmaecker Winnok H.De Vos Stefaan C.De Smedt Rabah Boukherroub jan Gettemans Ranhua Xiong Kevin Braeckmans Laboratory of General Biochemistry and Physical Pharmacy Faculty of PharmacyGhent UniversityGhent B-9000Belgium Department of Biomolecular medicine Faculty of Medicine and Health SciencesGhent UniversityGhent B-9000Belgium VIB-UGent Center for Inflammation Research VIBGhent B-9000Belgium VIB Bioimaging Core Ghent VIBGhent B-9000Belgium Department of Biomedical Molecular Biology Ghent UniversityGhent B-9000Belgium Univ.Lille CNRSCentrale LilleISENUniv.ValenciennesUMR 8520-IEMNLille F-59000Franc Laboratory of Cell Biology and Histology Department of Veterinary SciencesUniversity of Antwerp2020 AntwerpBelgium Centre for Advanced Light Microscopy Ghent UniversityGhent B-9000Belgium

Fluorescence microscopy is the method of choice for studying intracellular ***,its success depends on *** of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic *** to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live *** have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their *** a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for *** by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living *** illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B *** an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for ***-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and *** conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy.

关键词： laser-induced photoporation vapor nanobubble long-term microscopy imaging nanobody intracellular delivery living cell labeling

Evaluation of Peppermint Leaf Flavonoids as SARS-CoV-2 Spike Receptor-Binding Domain Attachment Inhibitors to the Human ACE2 Receptor: A Molecular Docking Study

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Open journal of Biophysics 2022年第2期12卷 132-152页

作者： Marcelo Lopes Pereira júnior Rafael timóteo de Sousa junior Georges Daniel Amvame Nze William Ferreira Giozza Luiz Antônio Ribeiro júnior Department of Electrical Engineering University of Brasília Brasília Brazil Institute of Physics University of Brasília Brasília Brazil

Virtual screening is a computational technique widely used for identifying small molecules which are most likely to bind to a protein target. In the present work, a molecular docking study is carried out to propose potential candidates for preventing the RBD/ACE2 attachment. These candidates are sixteen different flavonoids present in the peppermint leaf. Results showed that Luteolin 7-O-neohesperidoside is the peppermint flavonoid with a higher binding affinity regarding the RBD/ACE2 complex (about -9.18 Kcal/mol). On the other hand, Sakuranetin presented the lowest affinity (about -6.38 Kcal/mol). Binding affinities of the other peppermint flavonoids ranged from -6.44 Kcal/mol up to -9.05 Kcal/mol. The binding site surface analysis showed pocket-like regions on the RBD/ACE2 complex that yield several interactions (mostly hydrogen bonds) between the flavonoid and the amino acid residues of the proteins. This study can open channels for the understanding of the roles of flavonoids against COVID-19 infection.

关键词： Coronavirus Sars-CoV-2 Peppermint Flavonoids RBD/ACE2 Inhibitors

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恶劣环境下保护电路板安全

中国电子商情（空调与冷冻） 2005年第6期 30-32页

作者： j．Patrick Byrne tim Stampe Comco公司技术经理 Vansco电子公司高级服务技术专家

对那些由保形涂层保护的电路进行失效测试分析和质量控制评估，是非常困难的。而空气研磨设备的采用也许能够提供一种解决方案。本文专门探讨了在恶劣环境下实施空气研磨进行元件返工、评估和修理的利与弊。

关键词：恶劣环境涂层保护电路板安全研磨设备质量控制测试分析评估空气返工

血清乙型肝炎病毒DNA阳性与原发性肝癌危险性关系的前瞻性队列研究

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中华肝脏病杂志 2009年第12期17卷 930-934页

作者：陈钦艳董柏青杨进业韦少超方孔雄王学燕方钟燎 Caroline Sabin tim j Harrison 广西壮族自治区疾病预防控制中心病毒性疾病预防控制科、办公室、信息科南宁530028 广西壮族自治区隆安县疾病预防控制中心计划免疫科英国伦敦大学学院

目的探讨血清HBV DNA阳性与原发性肝癌的关系。方法以分层抽样方法对广西隆安县12个乡镇30～55岁农村居民抽样，采静脉血，酶联免疫吸附试验检测HBsAg，套式聚合酶链反应检测HBV DNA。根据HBsAg和DNA检测结果，将研究对象分为HBsAg（＋... 详细信息

目的探讨血清HBV DNA阳性与原发性肝癌的关系。方法以分层抽样方法对广西隆安县12个乡镇30～55岁农村居民抽样，采静脉血，酶联免疫吸附试验检测HBsAg，套式聚合酶链反应检测HBV DNA。根据HBsAg和DNA检测结果，将研究对象分为HBsAg（＋）／HBvDNA（＋）组（A组）和HBsAg（＋）／HBVDNA（-）组（B组）；根据1：1匹配原则，从本村屯HBsAg（-）者中为前两组观察对象挑选对照，组成HBsAg阴性对照组（C组），对这3组人群进行4年的前瞻性跟踪随访。卡方检验对各组及各因素的发病率进行统计分析，然后用Cox比例风险模型分析与PLC有关的危险因素，用后退法对数值进行迭代分析。结果30～55岁人群HBsAg阳性率为14．52％（3975／27379），HBsAg阳性者HBV DNA阳性率为40．35％（1604／3975）。A、B两组总的肝癌发病率为672．45／10万人年，明显高于C组的17．19／10万人年（P〈0．01），相对危险度为39．123，95％可信因司为9．018～159．146。A组肝癌发病率为984．03／10万人年，显明高于C组的324．38／10万人年（P〈0．01），相对危险度为3．034，95％可信区间为1．795～5．125。对A、B两组进行肝癌危险因素的多因素Cox模型分析，结果表明性别、年龄、血清HBV DNA阳性、肝癌家族史和以玉米为主食均为肝癌的危险因素。结论血清HBV DNA阳性可增加HBsAg阳性者的肝癌危险性。

关键词：肝炎病毒,乙型肝炎表面抗原,乙型 DNA,病毒肝肿瘤队列研究,前瞻性