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Compar.son of Detection of Antibodies with Yeast Lysate Antigens Pr.par.d fr.m Two Isolates of <i>Blastomyces der.atitidis</i>by Two Differ.nt Methods: Sensitivity and Specificity Evaluations

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Open Jour.al of Medical Micr.biology 2012年第4期2卷 142-146页

作者： tiffany r. allison Gene M. Scalar.ne Depar.ment of Biological Sciences Idaho State University Pocatello USA

Blastomycosis, a systemic fungal disease, caused by the dimor.hic fungus Blastomyces der.atitidis, has continually pr.sented clinicians with concer.s with r.gar. to labor.tor. diagnosis and pr.vention. For.year. r.sear.her. have str.ved to develop antigens with a high degr.e of sensitivity and specificity. The pur.ose of this study was to gain a bet- ter.under.tanding of how two novel yeast lysate antigens, pr.par.d fr.m two B. der.atitidis isolates by differ.nt meth- ods, would be able to detect antibody r.sponses in immunized r.bbits in a specific and sensitive manner. The en- zyme-linked immunosor.ent assay (ELISA) was used to evaluate antibody in ser.m specimens with yeast lysate r.- agents pr.par.d after.allowing yeast cells to lyse for.1 or.7 days. The r.sults indicated that r.activity was gr.ater.with the day 7 antigens, with both the B5896 and 597 B. der.atitidis isolates, when compar.d to the day 1 antigens;in con- tr.st the day 1 pr.par.tions exhibited less cr.ss r.activity when assayed against anti-Histoplasma capsulatum ser.m specimens.

关键词： Isoelectr.c Focusing ELISA Blastomycosis Lysate Antigen Antibody Detection

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Blastomyces der.atitidis: Stability studies on differ.nt yeast lysate antigens

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Open Jour.al of Immunology 2013年第3期3卷 98-102页

作者： tiffany r. allison Joshua C. Wr.ght Gene M. Scalar.ne Depar.ment of Biological Sciences Idaho State University Pocatello USA

In Tr.al 1, 19 lots of Blastomyces der.atitidis (T-58;Tennessee dog isolate) wer. assayed to deter.ine the stability of the r.agents following stor.ge. The r.activity of the antigens, pr.duced fr.m 1989 to 2012 and stor.d at 4°C, was deter.ined by compar.ng antibody detection (enzyme-linked immunosor.ent assay;ELISA) in 12 ser.m specimens fr.m immunized r.bbits. All of the 19 r.agents pr.duced dur.ng this 23-year.per.od exhibited a high degr.e of stability and wer. able to detect antibody in the ser.. Mean absor.ance values r.nged fr.m 0.798 (1989) to 0.827 (2012) and a mean value for.all 19 antigens of 0.728. In a r.lated evaluation, Tr.al 2, B. der.atitidis lysate antigens pr.par.d fr.m 8 isolates (dog, human, soil) at two differ.nt time per.ods wer. assayed as above to deter.ine r.activity. The time of stor.ge between the fir.t and second r.agents var.ed fr.m 4 to 17 year.. The r.sults indicated that all 16 of the lysate antigens detected antibody in the 15 r.bbit ser.m specimens with mean absor.ance values r.nging fr.m 0.346 to 0.682, but var.ations in r.activity wer. obser.ed depending on the lysate and the ser.m specimen assayed. This compar.tive study pr.vided evidence that the antigenic r.agents do exhibit some lot-to-lot var.ation in r.activity, but they did not lose any appr.ciable potency dur.ng pr.longed stor.ge.

关键词： Blastomyces der.atitidis Lysate Antigens Antibody Detection Stability ELISA

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