During early stage of embryonic development,the liver bud,arising from the foregut endoderm, is the beginning for the formation of future liver three-dimensional *** the gene expression profiles associated with this d...
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During early stage of embryonic development,the liver bud,arising from the foregut endoderm, is the beginning for the formation of future liver three-dimensional *** the gene expression profiles associated with this developmental stage have been well explored,the detailed cellular events are not as ***-mesenchymal transition(EMT) was thought to be essential for cell migration in the early vertebrate embryo but seldom demonstrated in human liver *** this study,we tried to identify the cell populations with both stem cell and EMT features in the human liver *** in situ studies show that the phenotype of EMT occurs at initiation of human liver development,accompanied by up-regulation of EMT associated genes.A human liver bud derived stem cell line(hLBSC) was established, which expressed not only genes specific to both mesenchymal cells and hepatic cells,but also Octamer-binding protein 4(OCT4) and *** in appropriate media,hLBSC differentiated into hepatocytes,adipocytes,osteoblast-like cells and neuron-like cells in *** transplanted into severe combined immunodeficiency mice pre-treated by carbon tetrachloride,hLBSC engrafted into the liver parenchyma and *** data suggest that mere are cell populations with stem cell and EMT-like properties in the human liver bud,which may play an important role in the beginning of the spatial structure construction of the liver.
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