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Self-confocal NIR-II fluorescence microscopy for multifunctional in vivo imaging

Journal of Innovative Optical Health Sciences 2024年第1期17卷 105-119页

作者： Jing Zhou tianxiang wu Runze Chen Liang Zhu Hequn Zhang Yifei Li Liying Chen Jun Qian State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic ResearchCollege of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou 310058P.R.China Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine Zhejiang UniversityHangzhou 310058P.R.China College of Biomedical Engineering and Instrument Science Interdisciplinary Institute of Neuroscience and Technology(ZIINT)Zhejiang UniversityHangzhou 310027P.R.China

Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial ***,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to *** types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this *** type is-ber-pinhole-based confocal microscope applicable to CW laser *** is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser *** can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence ***,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.

关键词： Self-confocal fiber-pinhole air-pinhole multi-channe fluorescence lifetime imaging multi-color imaging

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天麻参与灰树花固态发酵对纤维素关键酶活力和不同发酵期菌质挥发性成分变化的研究

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食品与发酵工业 2024年第17期50卷 91-96页

作者：吴力亚吴天祥张榕欣茂名职业技术学院化学工程系广东茂名525000 贵州食品工程职业学院粮油食品工程学院贵州贵阳551400

真菌参与中药的发酵不仅可以改变药理成分的结构和功效,而且中药中所含的特有药理成分能够通过改变与真菌生长代谢相关酶的活性影响真菌的生长代谢。该研究以中药天麻(Rhizoma gastrodiae)作为灰树花(Grifola frondosa)固态发酵的外源... 详细信息

真菌参与中药的发酵不仅可以改变药理成分的结构和功效,而且中药中所含的特有药理成分能够通过改变与真菌生长代谢相关酶的活性影响真菌的生长代谢。该研究以中药天麻(Rhizoma gastrodiae)作为灰树花(Grifola frondosa)固态发酵的外源添加物,探究天麻参与灰树花固态发酵对其纤维素关键酶的影响以及不同阶段菌质挥发性成分的变化规律。结果表明,天麻添加量为40%,能够显著促进灰树花生长,其次天麻添加量为10%~50%,对其纤维素关键酶的活力均有促进效果,并且添加量为40%时,滤纸酶、外切葡聚糖酶、淀粉酶活力均达到其最佳促进效果,三者酶活力分别为空白组(未添加天麻)的1.78倍、0.61倍和1.19倍。在发酵过程中,天麻成分巴利森苷含量显著提高。运用顶空固相微萃取结合气相色谱-质谱联用(headspace solid-phase microextraction combined with gas chromatography-mass spectrometry,HS-SPME-GC-MS)技术对不同阶段菌质挥发性成分进行检测,共检测到276种挥发性成分,且发酵过程中酯类、醛类和醇类种类增加,3类呈香成分发酵12 d后分别增加了15、8、4种,发酵显著提高了菌质挥发性化合物的种类和含量。中药天麻与真菌灰树花共发酵可以提高真菌纤维素关键酶活力,提高药效成分巴利森苷的含量,综上所述,改变菌质挥发性成分的种类和含量进而改善产品的风味。通过天麻与灰树花的共发酵丰富了中药资源生物增效的途径,促进中药和食用菌资源的协调开发利用。

关键词：灰树花天麻胞外酶固态发酵挥发性成分

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健身气功在职业院校公体课教学中的价值和路径研究

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当代体育科技 2024年第20期14卷 102-105页

作者：吴天祥菏泽家政职业学院山东菏泽274300

公体课中开展健身气功课程,对于增强学生体质、提高身心健康水平、培养良好的体育习惯以及形成终身体育的意识等有积极的促进作用,同时,学生可以深入了解体验中华传统文化的魅力,增强对传统文化的认同感和自豪感。如何采取策略促进健身... 详细信息

公体课中开展健身气功课程,对于增强学生体质、提高身心健康水平、培养良好的体育习惯以及形成终身体育的意识等有积极的促进作用,同时,学生可以深入了解体验中华传统文化的魅力,增强对传统文化的认同感和自豪感。如何采取策略促进健身气功在职业院校公体课中的融入和开展,实现健身气功的文化传承,以及发挥其健身强魄的价值,成为亟待研究和解决的问题。以职业院校公体课为切入点,运用文献资料法、专家访谈法、逻辑分析法等研究方法,对健身气功在职业院校公体课教学中的价值和路径展开研究,以期为健身气功在职业院校公体课中的应用提供一些帮助。

关键词：健身气功职业院校公体课教学价值

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RP11-789C1.1 inhibits gastric cancer cell proliferation and accelerates apoptosis via the ATR/CHK1 signaling pathway

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中华医学杂志(英文版) 2024年第15期137卷 1835-1843页

作者： Liu Wenwei Feng Wei Zhang Yongxin Lei tianxiang Wang Xiaofeng Qiao Tang Chen Zehong Song wu Digestive Diseases Center The Seventh Affiliated Hospital of Sun Yat-sen University Shenzhen Guangdong China Department of Gastrointestinal Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong China Laboratory of General Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong China Department of Gastrointestinal Surgery The Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong China

Background: Long non-coding RNAs (lncRNAs) plays an important role in the progression of gastric cancer (GC). Their involvement ranges from genetic regulation to cancer progression. However, the mechanistic roles of RP11-789C1.1 in GC are not fully ***: We identified the expression of lncRNA RP11-789C1.1 in GC tissues and cell lines by real-time fluorescent quantitative polymerase chain reaction. A series of functional experiments revealed the effect of RP11-789C1.1 on the proliferation of GC *** vivo experiments verified the effect of RP11-789C1.1 on the biological behavior of a GC cell line. RNA pull-down unveiled RP11-789C1.1 interacting proteins. Western blot analysis indicated the downstream pathway changes of RP11-789C1.1, and an oxaliplatin dosing experiment disclosed the influence of RP11-789C1.1 on the drug sensitivity of ***: Our results demonstrated that RP11-789C1.1 inhibited the proliferation of GC cells and promoted the apoptosis of GC cells. Mechanistically, RP11-789C1.1 inhibited checkpoint kinase 1 (CHK1) phosphorylation by binding ataxiatelangiectasia mutated and Rad3 related (ATR), a serine/threonine-specific protein kinase, promoted GC apoptosis, and mediated oxaliplatin ***: In general, we discovered a tumor suppressor molecule RP11-789C1.1 and confirmed its mechanism of action, providing a theoretical basis for targeted GC therapy.

关键词： Long non-coding RNA RP11-789C1.1 Stomach neoplasms ATR CHK1 Oxaliplatin

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Tuina alleviates neuropathic pain through regulate the activation of microglia and the secretion of inflammatory cytokine in spinal cord

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Journal of Traditional Chinese Medicine 2024年第4期44卷 762-769页

作者： wu Zhiwei ZHU Qingguang KONG Lingjun SONG Pengfei ZHOU Xin GUO Guangxin ZHANG Shuaipan HE tianxiang CHENG Yanbin FANG Min Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Institute of Tuina Shanghai Institute of Traditional Chinese MedicineShanghai 200437China Shuguang Hospital Shanghai University of Traditional Chinese MedicineShanghai 200021China Department of Acupuncture and Tuina Shanghai University of Traditional Chinese MedicineShanghai 201203China

OBJECTIVE:To observe the analgesic effects of Tuina on neuropathic pain(NPP)and the underlying ***:Forty-eight Sprague-Dawley(SD)rats were assigned by random into three treatment groups:sham,chronic constriction injury(CCI),and *** group contained sixteen *** model was generated by ligating the right sciatic *** changes of CCI were assessed by the paw withdrawal threshold(PWT)and paw withdrawal latency(PWL).In addition,biochemical techniques such as immunofluorescence staining,enzyme-linked immunosorbent assay(ELISA)and Western blotting were used to profile levels of microglia activation and inflammatory factors in the spinal dorsal horn(SDH)of ***(clockwise pressing and rubbing)was performed at Chengshan(BL57)to observe the analgesic effects on CCI rats and the underlying ***:Rats with CCI experienced significant reduction in the PWT and PWL of the right hind paw relative to CCI group at day *** treatment rescued this situation significantly on days 10 and ***,Iba-1,microglia M1 receptor CD68,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)were higher in the right SDH for CCI group compared to the sham group on day *** expected,Tuina partially downregulated the CCI-induced overexpressed Iba-1,CD68,TNF-α,and IL-1βin the SDH of CCI ***:Tuina induces a time-dependent cumulative analgesic effect in CCI rats by inhibiting the activation of microglia and the secretion of IL-1βand TNF-αin SDH.

关键词： Tuina neuralgia microglia inflammatory cytokine spinal cord

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A Multilayered Magnetoelectric Transmitter with Suppressed Nonlinearity for Portable VLF Communication

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Research 2024年第2期2023卷 553-563页

作者： Zhaoqiang Chu Zhineng Mao Kaixin Song Shizhan Jiang Shugang Min Wei Dan Chenyuan Yu Meiyu wu Yinghui Ren Zhichao Lu Jie Jiao tianxiang Nan Shuxiang Dong Qingdao Innovation and Development Base Harbin Engineering UniversityQingdao 266071China College of Underwater Acoustics Engineering Harbin Engineering UniversityHarbin 150001China Songshan Lake Materials Laboratory DongguanGuangdong 523808 China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 201800China School of Integrated Circuits and Beijing National Research Center for Information Science and Technology(BNRist) Tsinghua UniversityBeijing 100084China College of Engineering Peking UniversityBeijing 100871China

Acoustically actuated magnetoelectric(ME)antenna based on the efficient oscillation of magnetic dipoles has recently been considered as a promising solution for portable very-low-frequency ***,the severe nonlinear dynamic behavior in the case of strong-field excitation results in insufficient radiation capability and poor communication performance for a conventional ME *** this work,we propose to suppress the nonlinearity of an ME antenna by neutralizing the spring-hardening effect in amorphous Metglas and the spring-softening effect in piezoelectric ceramics through an ME multilayered transmitter(ME-MLTx)*** a driving voltage of 50 V_(pp)at the resonance frequency of 21.2 kHz,a magnetic flux density as high as 108 fT at a distance of 100 m is produced from a single *** addition,ME-MLTx performs a decreased mechanical quality factor(Q_(m))less than 40.65,and,thus,a broadened bandwidth of 500 Hz is ***,a communication link transmitting binary American Standard Code for Information Interchange-coded message is built,which allows for an error-free communication with a distance of 18 m and a data rate of 300 bit/s in the presence of heavy environment *** communication distance can be further estimated over 100 m when using a femtotesla-class-inductive magnetic field *** obtained results are believed to bring ME antennas one step closer to being applicable in very-low-frequency communications.

关键词： closer nonlinearity inductive

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煤矿区循环经济发展初探

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中国煤炭 2010年第11期36卷 38-39页

作者：吴天翔中国煤炭进出口公司北京市东城区100011

阐述了我国煤矿发展循环经济的意义,结合平朔矿区示范工程,给出煤矿发展循环经济的一种模式。

关键词：生态工业园循环经济煤炭矿区

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天麻参与灰树花固态发酵对基质主要活性成分的影响

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食品与发酵工业 2021年第16期47卷 40-45页

作者：吴力亚吴天祥汪玲贵州大学酿酒与食品工程学院贵州贵阳550025 贵州食品工程职业学院贵州贵阳550025

以药食同源原料苦荞、薏仁米等为主要基质,利用灰树花(Grifola frondosa)作为发酵菌种实现固态发酵,试研究菌种对此类发酵基质主要活性成分的分解情况,添加不同比例的天麻(Rhizoma gaetrodiae)参与灰树花的固态发酵,探究天麻对发酵基质... 详细信息

以药食同源原料苦荞、薏仁米等为主要基质,利用灰树花(Grifola frondosa)作为发酵菌种实现固态发酵,试研究菌种对此类发酵基质主要活性成分的分解情况,添加不同比例的天麻(Rhizoma gaetrodiae)参与灰树花的固态发酵,探究天麻对发酵基质中总黄酮分解程度以及灰树花对基质中中药天麻所含天麻素(p-hydroxymethylphenyl-β-D-glucopyranoside,GA)、对羟基苯甲醇(p-hydroxybenzyl alcohol,HA)、对羟基苯甲醛(p-hydroxylbenzaldehyde,HBA)、巴利森苷(parishin)的影响。研究表明,天麻添加量为40%(质量分数)时,对促进灰树花分解利用总黄酮影响最为显著,与空白组相比,总黄酮含量分解程度提高了1.3倍(P<0.01),对发酵基质中芦丁及异槲皮苷成分进行检测,结果表明灰树花在发酵期间能够对黄酮类物质芦丁和异槲皮苷进行分解,使2种黄酮类物质含量明显降低。对天麻4种主要成分进行测定,结果表明,天麻添加量在10%~50%时,天麻素会完全被灰树花分解;天麻添加量为30%时,与对照组相比,灰树花分解对羟基苯甲醇的能力提高了3.19倍;不同比例天麻参与发酵对对羟基苯甲醛的分解均有抑制作用,均能够显著提高巴利森苷的含量。

关键词：灰树花天麻苦荞固态发酵生物转化

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茯苓提取物参与灰树花液态深层发酵及对其活性物质产量的影响

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食品与发酵工业 2021年第5期47卷 57-62页

作者：吴力亚吴天祥汪玲贵州大学酿酒与食品工程学院贵州贵阳550025 贵州食品工程职业学院贵州贵阳550025

中药茯苓提取物参与灰树花液态深层共发酵,进而探究不同溶剂提取的、不同添加比例的茯苓提取物对灰树花活性物质产量的影响。结果表明,添加5 g/L的茯苓醇提液和7 g/L的茯苓水提液时,均能够提高灰树花的生物量及多糖、总黄酮、蛋白的含... 详细信息

中药茯苓提取物参与灰树花液态深层共发酵,进而探究不同溶剂提取的、不同添加比例的茯苓提取物对灰树花活性物质产量的影响。结果表明,添加5 g/L的茯苓醇提液和7 g/L的茯苓水提液时,均能够提高灰树花的生物量及多糖、总黄酮、蛋白的含量。相比于空白对照组,在最适醇提物和水提物添加量下的菌丝体生物量分别提高了24.28%和29.04%,具有显著促进作用(P<0.05)。5 g/L醇提物添加条件下的胞外多糖、胞内多糖、胞外黄酮、胞内黄酮、胞外蛋白、胞内蛋白组与空白组相比具有显著促进作用(P<0.05),分别提高了17.8%、30.29%、8.32%、10.13%、10.49%和10.24%。7 g/L水提液添加条件下胞内多糖、胞外黄酮、胞内黄酮、胞外蛋白、胞内蛋白与空白组相比具有显著促进作用(P<0.05),分别提高了19.8%、3.16%、9.29%、10.24%和8.78%。表明添加一定量的茯苓提取物能够在一定程度上增加灰树花菌丝体的生长和活性代谢产物的含量,并且对多糖的产量影响尤为明显。

关键词：灰树花液态发酵茯苓代谢产物:活性成分

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Establishment of Small Centralized Collection Processing Service System of Scattered Farming Waste

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Meteorological and Environmental Research 2015年第4期6卷 14-17页

作者： Bai Yanfei Wang Zichen wu Hao Wang Haiqin Shen Jianning wu tianxiang Guan Yongxiang Liang Yonghong Jiangsu Station of Agro-Ecological Monitoring and Protection Circular Agricultural Research Center Jiangsu Academy of Agricultural Sciences

In order to advance pollution control and promote agricultural source pollution reduction of Jiangsu Province, based on the practical expe- rience of Lijia Livestock and Poultry Manure Processing Center in wujin District, Changzhou City, Jiangsu Province, new ways of controlling small scattered livestock and poultry breeding pollution, with characteristics of ＂ unified collection, centralized processing, social services, and compre- hensive utilization＂, were discussed through establishing the collection processing service system, innovating operation system and mechanism, and transforming the mode of government supervision and management.

关键词： Small scale Livestock and poultry breeding Collection System Comprehensive utilization China

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