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脊尾白虾生长和繁殖性状的遗传参数估计

渔业科学进展 2024年第6期45卷 155-166页

作者：王成伟王佳佳张秀红唐帅王培春李健李吉涛上海海洋大学水产科学国家级实验教学示范中心上海201306 海水养殖生物育种与可持续产出全国重点实验室(中国水产科学研究院黄海水产研究所) 山东青岛266071 青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室山东青岛266237 日照海辰水产有限公司山东日照276800

为估计脊尾白虾(Exopalaemon carinicauda)生长和繁殖性状的遗传参数,本研究采用巢式交配设计建立家系,构建了40个脊尾白虾全同胞家系,其中,包括25个半同胞家系,利用一般线性模型估计了脊尾白虾50日龄和80日龄体长、总腹节长、体重以及... 详细信息

为估计脊尾白虾(Exopalaemon carinicauda)生长和繁殖性状的遗传参数,本研究采用巢式交配设计建立家系,构建了40个脊尾白虾全同胞家系,其中,包括25个半同胞家系,利用一般线性模型估计了脊尾白虾50日龄和80日龄体长、总腹节长、体重以及抱卵量、单位体重抱卵量、单位体长抱卵量和第1次产卵时间性状的遗传力、表型相关和遗传相关。结果显示,50日龄体长、总腹节长、体重的遗传力分别为0.27~0.69、0.31~0.44和0.20~0.65,80日龄体长、总腹节长和体重的遗传力分别为0.39~0.54、0.31~0.47、0.33~0.71;抱卵量、单位体重抱卵量、单位体长抱卵量和第1次产卵时间的遗传力分别为0.45~0.52、0.30~0.49、0.43~0.48和0.33~0.77;经过t检验,全同胞方差组分估计的遗传力达到极显著水平(P1)。因此,脊尾白虾的生长和繁殖性状的狭义遗传力的无偏估计值为全同胞方差组分估计的遗传力。体重与体长、抱卵量性状的遗传相关系数分别为0.951 4和0.205 8,体重与体长、抱卵量性状的表型相关系数分别为0.742 0和0.212 4。研究表明,脊尾白虾体长、总腹节长及体重3个生长性状和抱卵量、单位体长抱卵量、单位体重抱卵量及第1次产卵时间4个繁殖性状均达到中高等遗传力水平,体重和体长性状为高度正相关,体重和抱卵量性状为低度正相关。本研究为脊尾白虾高繁殖力性状良种选育工作提供了数据参考。

关键词：脊尾白虾生长性状繁殖性状遗传力遗传相关

Isolation of Growth Hormone-Releasing Hormone and Its Receptor Genes from Scatophagus argus and Their Expression Analyses

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Journal of Ocean University of china 2019年第6期18卷 1486-1496页

作者： JIANG Dongneng SHI Hongjuan LIU Qianqing wang Tuo HUANG Yuanqing HUANG Yang DENG Siping CHEN Huapu tian Changxu ZHU Chunhua LI Guangli Guangdong research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species Key Laboratory of Marine Ecology and Aquaculture Environment of ZhanjiangKey Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education InstitutesFisheries College Guangdong Ocean University

The teleost Scatophagus argus is a species whose females grows faster than *** hormone(gh)mRNA abundance in females pituitary is higher than that in males;however the mechanism underlining such differential is still *** hormone(GH)is tightly associated with GH-releasing hormone(Ghrh)in *** this study,Ghrh gene(ghrh)and its receptor gene,ghrhr,were isolated from *** expression analysis showed that ghrh and ghrhr were mainly expressed in hypothalamus while ghrhr was expressed in pituitary and gh was predominantly expressed in *** cultured *** individuals were used to compare ghrh,ghrhr and gh mRNA abundances,120 g and 181 g average weight for male(n=11)and female(n=9),***-time PCR indicated that the ghrh and ghrhr mRNA abundances in male hypothalamus were significantly higher than those in female hypothalamus while that of gh mRNA abundance was significantly higher in female pituitary than in male *** ghrh and ghrhr mRNA abundances were significantly up-regulated in female hypothalamus 3 h after injection of 0.1 mg kg^-1 body weight Ghrh while pituitary ghrhr and gh mRNA abundances were not *** female hypothalamus,ghrh and ghrhr m RNA abundances were not affected at 6 h post-injection of 4 mg kg^-1 body weight 17α-methyltes-tosterone(17α-MT)or 17β-Estradiol(E2).In female pituitary,ghrhr m RNA abundance was down-regulated by 17α-MT while that of gh m RNA abundance was up-regulated by *** findings indicated that E2,rather than Ghrh,plays an important role in up-regulating the expression of gh in female ***,which should aid to understand the sexual dimorphism of teleost growth.

关键词： Scatophagus argus growth hormone-releasing hormone growth hormone-releasing hormone receptor growth hormone estrogen sexual dimorphism

海南地区养殖石斑鱼皮肤溃疡病病原的分离、鉴定、药敏分析及病理学研究

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海南大学学报（自然科学版） 2018年第2期36卷 153-163页

作者：许悦王印庚周永灿张正廖梅杰李彬梁友海南大学海洋学院海南省热带水生生物技术重点实验室南海海洋资源利用国家重点实验室海南海口570228 中国水产科学研究院黄海水产研究所青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛266071

2016年,针对海南岛沿岸9个石斑鱼养殖场的石斑鱼皮肤溃疡病的调查发现,该疾病的发病面积广、死亡率高,对养殖产业的危害严重.从9个采样点3种患病石斑鱼样品的组织中分离得到了9株优势菌株,人工回感试验表明,其感染特征与自然病症一致,... 详细信息

2016年,针对海南岛沿岸9个石斑鱼养殖场的石斑鱼皮肤溃疡病的调查发现,该疾病的发病面积广、死亡率高,对养殖产业的危害严重.从9个采样点3种患病石斑鱼样品的组织中分离得到了9株优势菌株,人工回感试验表明,其感染特征与自然病症一致,证明这9株菌均为皮肤溃疡病的致病原,在经过生理生化和16S r DNA序列分析后,这9株菌均被鉴定为哈维氏弧菌(Vibrio harveyi).人工回感试验证实,菌株SY-1对珍珠龙胆石斑鱼的毒力最强,其14天每克鱼体重的半致死剂量(LD50值)为7.18×10~4cfu;药敏实验的分析表明,这9株病原菌对34种抗菌药物的药敏谱基本一致,但对氟苯尼考、强力霉素、四环素等药物高度敏感;病理学的研究表明,患病鱼的鳃丝、皮肤、肌肉、肝脏、脾脏、肾脏、肠道和脑等组织器官都发生了不同程度的病理变化,因此认为该病为全身性感染.本研究表明,目前海南地区养殖石斑鱼皮肤溃疡病的致病原有较高的病原同一性,主要为哈维氏弧菌;而较早报道的溶藻弧菌、副溶血弧菌等致病菌的优势度大大降低了,其致病力有所下降,并且与海南地区养殖石斑鱼皮肤溃疡病的相关度不大.

关键词：石斑鱼海水养殖海南岛皮肤溃疡病哈维氏弧菌病理学全身性感染

Molecular Characterization and Expression and DNA Methylation Analyses of a Galectin-Related Protein Gene from Cynoglossus semilaevis

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Journal of Ocean University of china 2018年第5期17卷 1189-1196页

作者： XIU Yunji GAN tian XU Wenteng SHAO Changwei CHEN Songlin Laboratory for Marine fisheries science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine fisheries Ministry of Agriculture Marine science and Engineering College Qingdao Agricultural University College of fisheries and Life science Shanghai Ocean University

Galectins, a family of β-galactoside-binding proteins, participate in both innate immunity and adaptive immunity. This study identified one novel galectin-related protein from half-smooth tongue sole Cynoglossus semilaevis, which was designated as CsGRP. The full-length cDNA of its encoding gene was 785 bp in length with a 528 bp open reading frame encoding a putative protein of 176 amino acids. The deduced CsGRP contained a putative 131-aa galactoside-binding lectin domain and 3 critical residues responsible for carbohydrate binding （R93, W109 and R114）. Genomic structural analysis revealed that CsGRP consisted of five exons and four intzons. CsGRP showed 68% similarity with Poecilia latipinna GRP and 67% similarity wih Stegastes partitus GRP. CsGRP showed the highest expression level in liver, although its expression was detected in all tested tissues. When challenged with Vibrio harveyi, the expression of CsGRP was significantly down-regulated in liver （P〈 0.05）. In addition, we found that in spleen and kidney of C. semilaevis, the CpG island of CsGRP showed significantly higher （P 〈 0.05） methylation level in disease-resistant family of C. semilaevis （DR-Cs） than in disease-susceptible ones （DS-Cs）. Our results suggested that CsGRP may play important roles in the immune response of C. semilaevis. Moreover, DNA methylation patterns provided valuable data for understanding the relationship between epigenetic regulation and immunity, which would assist the animal genetic research and improve the animal breeding in the future.

关键词： Cynoglossus semilaevis galectin-related protein expression pattern DNA methylation immune response

Developmental Dynamics of the Larval Muscle System of Bay Scallop(Argopecten irradians)

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Journal of Ocean University of china 2020年第2期19卷 386-392页

作者： SUN Xiujun LIU Zhihong ZHOU Liqing WU Biao YANG Aiguo tian Jiteng Key Laboratory of Sustainable Development of Marine fisheries of Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine fisheries science and Food Production Processes Pilot National Laboratory for Marine Science and TechnologyQingdao 266071China

Though the larval development of bivalves has been extensively studied for commercial purposes,the dynamic development of larval muscle system remains largely *** this study,we characterized the larval muscle system at different developmental stages(D-shaped veligers,umbo veligers and spats)in the bay scallop(Argopecten irradians)by phalloidin staining and under a confocal *** functional muscles are initially established at the early stage of veligers,which have four pairs of velar retractors and one anterior *** the veliger stage,the velum and posterior retractor muscles are functionally important for velar contractility but undergo an irreversible shrink until they disappear at the end of the larval *** metamorphosis,three crucial modifications take place in the larval muscle *** metamorphosis process involves the gradual degeneration of velum retractors,mantle margin development from an unfolded to a three-fold state,and remodeling of the adductor muscle system from dimyarian(two adductors)to monomyarian condition(one adductor)as in juveniles/*** retractor muscles are composed of striated muscle,but both anterior and posterior adductors have smooth and striated *** findings highlight that the morphological changes at different stages are typical features of myogenesis in *** present knowledge on the developmental dynamics of myogenesis in the bay scallop will not only improve our understanding of phenotypic diversity of larval myoanatomy in bivalves,but also provide useful information on the larval culture in hatcheries.

关键词： myogenesis retractor muscle metamorphosis adductor muscle

眼斑双锯鱼(Amphiprion ocellaris)发育中体色花纹时序发生的色素细胞变化和控制基因表达的分析Ⅰ.胚胎时期

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海洋与湖沼 2024年第2期55卷 489-498页

作者：孙伟恒孙志宾王新安马爱军李迎娣苟冬惠于宏李昊喆 Vorathep Muthuwan 曲江波洪宜展大连海洋大学水产与生命学院辽宁大连116023 海水养殖生物育种与可持续产出全国重点实验室、中国水产科学研究院黄海水产研究所、中国-东盟海水养殖技术“一带一路”联合实验室(青岛) 山东青岛266071 布拉帕大学海洋科学研究所泰国春武里20131 烟台开发区天源水产有限公司山东烟台264006 海南省展渔生物科技有限公司海南乐东572536

眼斑双锯鱼(Amphiprion ocellaris)属于鲈形目、雀鲷科、双锯鱼属,是热带珊瑚礁观赏鱼类的首选品种。不同体色和花纹与色素细胞相关基因的表达直接相关,在其胚胎发育的过程中,色素细胞的形态和数量一直在变化,但不同发育时期各种色素细... 详细信息

眼斑双锯鱼(Amphiprion ocellaris)属于鲈形目、雀鲷科、双锯鱼属,是热带珊瑚礁观赏鱼类的首选品种。不同体色和花纹与色素细胞相关基因的表达直接相关,在其胚胎发育的过程中,色素细胞的形态和数量一直在变化,但不同发育时期各种色素细胞的动态变化及其控制基因表达情况等仍需明确研究。记录了眼斑双锯鱼胚胎时期色素细胞变化较为明显的6个发育时期,观察了不同时期的体色及色素细胞的变化特点,并利用荧光定量PCR检测了各发育时期的10个体色控制基因的表达情况。结果显示:在胚胎发育阶段,整个胚胎的颜色从橙黄逐渐转变为暗红、黑红、黑色到最终透明,观察到卵黄囊表面幼体黑色素细胞到成体黑色素细胞的转变过程,鱼体表面在翻转期出现成体黑色素细胞、在器官形成期出现红色素细胞,眼睛及腹部在孵化期出现虹彩色素细胞。结合荧光定量PCR结果分析发现:卵黄囊表面幼体黑色素细胞推测是外胚层中的神经嵴细胞直接发育而成,不需要迁移过程;Ednrb、TYR、Tbx2b基因对幼体黑色素细胞的形成和发育有重要作用,Pax3、Dct、Aim1基因与成体黑色素细胞的分化、迁移、形成过程密切相关;其中TYR基因的相对表达量在体节期出现显著增加,说明TYR基因在胚胎发育初期就参与黑色素细胞的形成;Tbx2b基因不仅对黑色素细胞形成有影响,对眼斑双锯鱼心脏的形成也有一定作用;Fms基因对红色素细胞形成有重要作用;Ltk基因的表达要早于虹彩色素细胞的出现;相关性分析发现各基因之间存在相互作用。

关键词：眼斑双锯鱼(Amphiprion ocellaris) 发育体色色素细胞表达分析

福建东吾洋海域海水和沉积物菌群结构及其与环境因子相关性

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渔业科学进展 2024年第2期45卷 186-198页

作者：张铭洋李昊王印庚马翠萍陈夫山黄强廖梅杰李彬王锦锦葛建龙荣小军王与凡青岛科技大学海洋科学与生物工程学院山东青岛266042 中国水产科学研究院黄海水产研究所山东青岛266071 霞浦县海洋渔业发展中心福建宁德355100

福建东吾洋海域是我国刺参(Apostichopus japonicus)吊笼养殖的核心产区,但目前该地区的水域环境现状和环境菌群结构相关研究匮乏。本研究以东吾洋海域东安、马坑、雷江和沙湾4个主要养殖区域为对象,完成了海参养殖高峰期1月的水质环境... 详细信息

福建东吾洋海域是我国刺参(Apostichopus japonicus)吊笼养殖的核心产区,但目前该地区的水域环境现状和环境菌群结构相关研究匮乏。本研究以东吾洋海域东安、马坑、雷江和沙湾4个主要养殖区域为对象,完成了海参养殖高峰期1月的水质环境因子调查和评价分析,采用高通量测序方法完成了水体和沉积物菌群多样性和差异特征菌群分析,解析了环境因子与菌群结构相关性。结果表明,马坑海域的富营养化指数为21.60,属于严重富营养化;东安和雷江海域属于重度富营养,只有沙湾海域处于中度富营养的水平。东安和沙湾海域水质属于轻度有机污染,而马坑和雷江海域属于中度有机污染。高通量测序共获得1 520个操作分类单元(operational taxonomic units, OTUs),归属于28门、57纲、163目、322科、581属。菌群结构多样性分析表明,各区域沉积物的菌群多样性水平均显著高于相应海域水体菌群多样性,且具有较强的空间异质性。沙湾海域环境菌群多样性与丰度最高。PCo A分析表明,沉积物和水体中细菌群落结构差异极显著,水体交换和海域位置对其水体和沉积物菌群结构的影响较大。具有潜在致病性的弧菌科(Vibrionaceae)在所有水体和沉积物样本中均为优势种类之一。对水体样品和沉积物样品的LEFSe(line discriminant analysis effect size)分析筛选到处于不同分类水平的74个特异菌群。基因功能的预测和COG分类统计表明,水体与沉积物样品在12个代谢途径上存在显著差异(P<0.05)。对4个海区的水体菌群LEFSe分析筛选到处于不同分类水平的30个特异菌群。水体的菌群与环境因子的RDA分析揭示,温度、溶氧、叶绿素a浓度是影响不同海域水体菌群结构的主要环境因子,相关研究将为福建刺参养殖管理提供基础数据和科学参考。

关键词：东吾洋海域刺参吊笼养殖环境评价菌群结构分析

基于溶藻弧菌感染的虾苗细菌性玻化症(BVS)及其防控中药筛选和药效学评价

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渔业科学进展 2024年第4期45卷 175-186页

作者：赵伟志王印庚于永翔张正朱洪洋庞明海王春元上海海洋大学水产与生命学院上海201306 中国水产科学研究院黄海水产研究所青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室山东青岛266071

为了筛选可用于防治虾苗细菌性玻化症(bacterial vitrified syndrome,BVS)的中药复方,本研究首先通过牛津杯法和二倍稀释法进行体外抑菌实验,从50种中药中筛选出对BVS致病菌溶藻弧菌(Vibrio alginolyticus)和副溶血弧菌(***)具有良好抑... 详细信息

为了筛选可用于防治虾苗细菌性玻化症(bacterial vitrified syndrome,BVS)的中药复方,本研究首先通过牛津杯法和二倍稀释法进行体外抑菌实验,从50种中药中筛选出对BVS致病菌溶藻弧菌(Vibrio alginolyticus)和副溶血弧菌(***)具有良好抑菌效果的中药。然后,以不同浓度的溶藻弧菌浸浴感染虾苗,建立BVS病理模型;再通过体内药效学实验从死亡率、组织病理学和超微组织病理学角度比较不同中药复方对于患BVS虾苗的防治效果。体外抑菌实验结果显示,五味子(Schisandrae chinensis fructus)、马鞭草(Verbenae herba)、乌梅(Mume fructus)等中药对溶藻弧菌的抑菌圈直径可达12 mm以上;诃子(Chebulae fructus)、五味子、牡丹皮(Moutan cortex)等中药对副溶血弧菌的抑菌圈直径可达13mm以上。五倍子(Gallachinensis)、诃子、五味子、丁香(Caryophylliflos)对溶藻弧菌和副溶血弧菌的最小抑菌浓度(MIC)均≤12.5mg/mL,最小杀菌浓度(MBC)均≤50 mg/mL。结合香附(Cyperi rhizoma)、栀子(Gardeniae fructus)2味中药组成3种中药复方,处方1、处方2和处方3;以5×10^(4) CFU/mL的溶藻弧菌感染虾苗成功建立BVS病理模型,在此基础上采用药物治疗患病虾苗7d,各组死亡率由低至高依次为空白对照组、处方1组、20%氟苯尼考粉对照组、处方3组、处方2组和阳性对照组。处方1组虾苗死亡率显著低于阳性对照组(P2组、处方3组和20%氟苯尼考对照组虾苗死亡率低于阳性对照组,但差异不显著(P>0.05)。由此可见,处方1可显著降低患病虾苗死亡率。组织病理学和超微组织病理学研究显示,相比于其他感染组,处方1组虾苗肝胰腺病变程度明显较轻,肠道结构较为完整;虾苗肝小管上皮细胞的细胞膜、细胞核正常,线粒体、内质网丰富且基本正常。表明口服处方1可对患病虾苗的肝胰腺和肠道组织起到良好的保护作用,并优于处方2、处方3和20%氟苯尼考粉。综上所述,处方1(诃子30 g、五味子20 g、香附20 g)对溶藻弧菌感染的虾苗保护和治疗效果良好。本研究结果可为研制防控虾苗BVS专用中药提供依据,助力对虾养殖绿色高质量发展。

关键词：凡纳对虾细菌性玻化症(BVS) 病理模型中药复方药效学

Combining otolith elemental signatures with multivariate analytical models to verify the migratory pattern of Japanese Spanish mackerel(Scomberomorus niphonius) in the southern yellow sea

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Acta Oceanologica Sinica 2020年第12期39卷 54-64页

作者： Xindong Pan Zhenjiang Ye Binduo Xu Tao Jiang Jian Yang Jiahua Cheng Yongjun tian fisheries College Ocean University of ChinaQingdao 266003China Key Laboratory of Ecological Environment and Resources of Inland fisheries Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi 214081China East china sea fishery research institute Chinese Academy of Fishery SciencesShanghai 200090China Laboratory for Marine fisheries science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China Frontiers science Center for Deep Ocean Multispheres and Earth System Ocean University of ChinaQingdao 266100China

Japanese Spanish mackerel,Scomberomorus niphonius,is a commercially important,highly migratory species that is widely distributed throughout the northwestern Pacific ***,its life history and migratory patterns are only partially *** study used otolith chemistry to investigate the migratory pattern of *** in the southern yellow sea,an important fishing *** sections of otoliths from 15 age-1 spawning or spent individuals,comprising up to one complete migration cycle,were analyzed from the core to the margin by using laser ablation inductively coupled plasma mass *** ratios of the element to Ca were integrated with microstructural analysis to produce age-related elemental *** multielemental analysis of otolith composition with multivariate analytical models,we quantified structural changes in otolith chemistry *** revealed there were diverse changing patterns of otolith chemistry profiles for detected elements and the elements of Na,Mg,Sr and Ba were important for the chronological *** clusters were identified through chronological clustering,representing the five life stages from the early stage to the spawning *** of Ba:Ca ratio was most informative,showing a step-decreasing pattern in the first four stages and a rebound in the spawning *** results support the hypothesized migratory pattern of ***:hatching and spending their early life in the coastal sandy ridges system of the southern yellow sea,migrating northeastward and offshore for feeding during juvenile stage,aggregating in early October and migrating outward to the Jeju Island for wintering,and returning to the coastal waters for *** study demonstrated the value of life-history related otolith chemistry profiles combined with multivariate analytical models was a means to verify the migration patterns of *** at regional scales with potential application in fisheries assess

关键词： otolith chemistry Scomberomorus niphonius migratory pattern multivariate regression tree southern yellow sea