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Genetic parameters and response to selection for body weight in turbot(Scophthalmus maximus, Linnaeus)

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Acta Oceanologica Sinica 2018年第6期37卷 47-51页

作者： LYU Ding wang weiji LUAN Sheng HU Yulong GUAN Jiantao LI Zhixiang WU Huanhuan kong jie LIU Shoutang Wuxi fisheries College Nanjing Agricultural University Wuxi 214081 China Key Laboratory for Sustainable Utilization of Marine fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China yellow sea fisheries Limited Company Haiyang 265122 China

Genetic parameters and response to selection were estimated for harvest body weight in turbot. The data consisted of 10 952 individuals of 508 full-sib families from three generations（G0, G1, and G2）. The heritability estimates for G0, G1, and G2 were 0.11±0.08, 0.18±0.09, and 0.17±0.07, respectively. Over three generations, the heritability estimate was 0.19±0.04. Maternal and common environmental effects were 0.10±0.04, 0.14±0.04, and0.13±0.03 within each generation and 0.12±0.01 across generations. The selection differential in growth was 18.24 g in G0 and 21.19 g in G1 corresponding to an average of 19.72 g per generation. The genetic gains were also calculated, they were 22.06 g in G1 and 11.93 g in G2, corresponding to 6.36% and 3.52% body weight. The total genetic gain after two generations was 10.10% body weight, which indicated that the selective breeding program for the body weight trait in turbot was successful.

关键词： turbot genetic parameter selective breeding body weight Scophthalmus maximus

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不同区域与品种的海带中砷富集特征的差异

水产学报 2011年第9期35卷 1436-1440页

作者：舒本胜王飞久尚德荣宁劲松孙修涛赵艳芳汪文俊李涛翟毓秀大连海洋大学食品工程学院中国水产科学研究院黄海水产研究所

通过分析不同养殖海域、不同品种海带中无机砷和总砷含量在养殖期间的变化,研究海带中总砷及无机砷的富集规律。结果显示,在生长期内海带对总砷的富集呈增长趋势,而无机砷含量则逐渐降低,其占总砷的比例由3月份1.36%～1.47%下降到7月份... 详细信息

通过分析不同养殖海域、不同品种海带中无机砷和总砷含量在养殖期间的变化,研究海带中总砷及无机砷的富集规律。结果显示,在生长期内海带对总砷的富集呈增长趋势,而无机砷含量则逐渐降低,其占总砷的比例由3月份1.36%～1.47%下降到7月份的0.41%～0.57%;同一海域、不同品种的海带对总砷和无机砷的富集具有差异性;同时期在不同养殖海域采收的同品种海带总砷和无机砷含量差异显著。基于监测数据对海带中的无机砷进行分析,所有海带样品中无机砷含量远低于现行食品卫生标准中的限量要求,不对食用安全性造成影响。

关键词：海带总砷无机砷富集规律

Status of aquatic organisms resources and their environments in Yangtze River system(2017-2021)⋆

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Aquaculture and fisheries 2024年第5期9卷 833-850页

作者： Haile Yang Li Shen Yongfeng He Huiwu tian Lei Gao Jinming Wu Zhigang Mei Nian Wei Lin wang Tingbing Zhu Feifei Hu Jinling Gong Hongchun Du Xinbin Duan Huatang Deng Daoqun wang Fengyue Zhu Yunfeng Li Fan Wu Huijun Ru Yan Zhang Junyi Li Junlin Yang Yuntao Zhou Dongdong Fang yinping wang Danqing Lin Yanping Yang Peijie Li Silei Liu Jian Yang Ping Zhuang Sikai wang Tao Zhang Gang Yang Wenbo Yang Lilai Yuan Kun Cao Shuo Xu Huiyuan Liu Zhiqiang Liang Chongrui wang Hong Li Xiping Yuan Xin Yang yilong Fu Yanping Zhang Haixin Zhang Zhiying Tao Sheng wang Xiaoping Gao Binsong Jin Kemao Li Guojie wang Shenglong Jian yingqin Li Chenjiang Xue Chunyun Lei Shaowei Xue yi Sun Bin Zhu Ke Shao Xingkun Hu Meihua Xiong Jun Du Bin He Tao Yan yingying Huang Yuanchao Zou Biwen Xie Yongming wang Bin Li Fei Liu Yaoyao Zhang Fei Fan Zhijian wang Jing Huang Haoran Gu Hailong Ge Yan Dan Yan Li Shuqiao wang Chuang Zhang Lu Zhou Xue wang Sheng Zeng Yan Xiang Xugang He Jianhui Qin Chengxing Xia jie Hou yifu Shi Lifang Gao Zhiqiang Zhu Hongbao Shen Yun Du Xuejun Duan Jiawu Xiong Deguo Yang Shaoping Liu Zhaohui Ni Hui Zhang Kai Liu Feng Zhao yingren Li Jianwei wang Qiwei Wei Yangtze River fisheries research institute Chinese Academy of Fishery SciencesWuhan430223China institute of Hydrobiology Chinese Academy of SciencesWuhan430072China fishery Resource and Environment research Center Chinese Academy of Fishery SciencesBeijing100141China Key Laboratory of Freshwater fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China East china sea fisheries research institute Chinese Academy of Fishery SciencesShanghai200090China institute of fisheries Engineering Chinese Academy of Fishery SciencesBeijing100141China Hunan fisheries science institute Changsha410153China Jiangxi fisheries research institute Nanchang330039China Jiujiang academy of fishery sciences Jiujiang332000China School of Life sciences Nanchang UniversityNanchang330031China Qinghai Provincial fishery Technology Extension Center Xining810012China Yunnan academy of fishery sciences Kunming650111China institute of Hydroecology Ministry of Water Resources and Chinese Academy of SciencesWuhan430079China fisheries institute Sichuan Academy of Agricultural SciencesChengdu611731China School of Life sciences Neijiang Normal UniversityNeijiang641100China School of Life sciences Southwest UniversityChongqing400715China Chongqing fishery sciences research institute Chongqing401120China Guizhou fisheries research institute Guiyang550025China College of fisheries Huazhong Agricultural UniversityWuhan430072China Hubei fisheries science research institute Wuhan430070China Shaanxi fisheries research institute Xi’an710086China Innovation academy for Precision Measurement science and Technology Chinese Academy of SciencesWuhan430077China Nanjing institute of Geography and Limnology Chinese Academy of SciencesNanjing210008China Central South Inventory and Planning institute of State Forestry and Grassland Administration Changsha410014China

The Yangtze River is the mother river of *** promote the aquatic ecosystem protection of the great river,the Project of Yangtze fisheries Resources and Environment Investigation(2017-2021)supported by the Ministry of Agriculture and Rural Affairs,*** carried out by 24 institutes and universities that located in the Yangtze River basin surveys the status of(1)fish species composition and spatial distribution,(2)current fish abundance,(3)endangered fishes,(4)Yangtze finless porpoise,(5)aquatic eco-environments,(6)water-level fluctuation areas,(7)capture fisheries and recreational fisheries of the Yangtze River mainstream and 10 of its main tributaries,including Yalong River,Heng River,Min River(including Dadu River),Chishui River,Tuo River,Jialing River,Wu River,Han River,Dongting Lake and Poyang *** results showed that there were 443 fish species(belonging to 163 genus,37 families,and 18 orders)before 2017,but only 323 fish species(including 15 new recorded exotic species)were recorded in the project of *** them,Cyprinus carpio,Carassius auratus,Hypophthalmichthys molitrix,Pelteobagrus fulvidraco,Coilia brachygnathus,Silurus asotus,Saurogobio dabryi,Ctenopharyngodon idellus,Pelteobagrus nitidus,Hemiculter leucisculus,Siniperca chuatsi,Coreius heterodon,Culter alburnus,Parabramis pekinensis,and Aristichthys nobilis were the dominant species across the whole Yangtze River *** is estimated that there were 886 million individuals weighing 124.8 million kg,merely equivalent to 27.3%of the resources in 1950s,30.9%of the resources in 1960s,or 58.7%of the resources in *** the new list of protected fishes that recorded in the Yangtze River system,only 15 of 29 were collected in this project of *** gladius has been affirmed to be Extinct by International Union for Conservation of Nature(IUCN).The wild individuals of Tenualosa reevesii and Luciobrama macrocephalus have disappeared for many years and maybe have been extinct

关键词： Fishes Yangtze finless porpoise Resources Aquatic ecosystem fisheries Background investigation Yangtze river system

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污泥臭氧原位减量工艺中抗生素的去除

环境科学 2018年第4期39卷 1739-1747页

作者：汪鲁贲伟伟李彦刚强志民中国科学院生态环境研究中心北京100085 中国水产科学研究院黄海水产研究所海洋渔业环境与生物修复实验室青岛266071

建立了两套小试规模的序批式反应器,一套作为污泥臭氧原位减量系统,另一套作为控制系统.通过两系统的长期运行(90 d)考察了污泥臭氧原位减量工艺对9种典型抗生素(四环素、土霉素、强力霉素、诺氟沙星、氧氟沙星、环丙沙星、洛美沙星、... 详细信息

建立了两套小试规模的序批式反应器,一套作为污泥臭氧原位减量系统,另一套作为控制系统.通过两系统的长期运行(90 d)考察了污泥臭氧原位减量工艺对9种典型抗生素(四环素、土霉素、强力霉素、诺氟沙星、氧氟沙星、环丙沙星、洛美沙星、恩诺沙星和阿奇霉素)的去除效果.结果表明,进水中目标抗生素的存在(9种抗生素各100μg·L^(-1))并未对活性污泥去除COD、总氮、氨氮和总磷的效果产生显著影响.污泥臭氧原位减量系统出水中目标抗生素浓度在运行期间保持相对稳定,且与控制系统接近;但污泥中目标抗生素浓度则显著低于控制系统.质量衡算表明目标抗生素在两系统内的输入和输出逐渐达到平衡,臭氧降解和剩余污泥排放分别为减量和控制系统中目标抗生素的主要去除途径.污泥臭氧处理单元可以降解减量系统入水中83%的目标抗生素,而控制系统入水中82%的目标抗生素则随剩余污泥排放.因此,污泥臭氧原位减量工艺可以明显削减活性污泥系统中抗生素的排放,具有重要的实际意义.

关键词：活性污泥工艺臭氧污泥原位减量磷回收抗生素

First record of abnormal body coloration in a rockfish Sebastes koreanus(Scorpaenoidei:Sebastidae)from coastal water of china based on morphological characteristics and DNA barcoding

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Journal of Oceanology and Limnology 2024年第2期42卷 640-646页

作者： Ang LI Huan wang Changting AN Shufang LIU yellow sea fisheries research institute Chinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine fisheries science and Food Production Processes Laoshan LaboratoryQingdao 266071China

The first record of abnormal body coloration in Sebastes koreanus Kim and Lee,1994,from the yellow sea of china,was documented based on morphological characteristics and DNA *** two rockfish specimens were collected from the coastal waters of qingdao,china,and the whole body and all fins of them were *** the two red-colored rockfish,there were tiny deep red spots on each fin,2 red radial stripes behind and below the eyes and 1 large deep red blotch on the opercula,while the similar stripe and spot patterns are also present in the *** specimens with normal body *** countable characteristics of the two specimens are in the range of the morphometry of *** further clarify the species identity and taxonomic status of the two specimens,DNA barcode analysis was carried *** genetic distance between the red-colored rockfish and *** was 0,and the minimum net genetic distances between the red-colored rockfish and other Sebastes species except for *** were 3.0%,which exceeds the threshold of species *** phylogenetic analysis showed that the DNA barcoding sequences of the two red-colored rockfish clustered with the *** *** above results of DNA barcode analysis also support that the two red-colored rockfish could be identified as the species of *** mechanism of color variation in *** is desirable for further research and the species could be an ideal model to study the color-driven speciation of the rockfishes.

关键词： abnormal body coloration Sebastes koreanus coastal water of china yellow sea morphological characteristics DNA barcoding

凡纳滨对虾在低氧环境下存活性状的遗传参数评估

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渔业科学进展 2024年第1期45卷 138-147页

作者：段毓佳谭建栾生罗坤王宏杰隋娟孟宪红孔杰湖州师范学院浙江省水生生物资源养护与开发技术研究重点实验室、中国水产科学研究院水生动物繁育与营养重点实验室浙江湖州313000 中国水产科学研究院黄海水产研究所农业农村部海洋渔业与可持续发展重点实验室山东青岛266071 海洋渔业科学与食物产出过程功能实验室山东青岛266071 邦普种业科技有限公司山东潍坊261000

在水产养殖中,尤其是高密度养殖情况下,缺氧会短期内造成个体的大量死亡,从而影响生产效益。本研究以凡纳滨对虾(Litopenaeus vannamei)高抗和快大2个品系作为研究对象,利用高致死溶解氧(DO)胁迫的方式,对2个品系各41个家系共计6560尾... 详细信息

在水产养殖中,尤其是高密度养殖情况下,缺氧会短期内造成个体的大量死亡,从而影响生产效益。本研究以凡纳滨对虾(Litopenaeus vannamei)高抗和快大2个品系作为研究对象,利用高致死溶解氧(DO)胁迫的方式,对2个品系各41个家系共计6560尾幼虾进行低氧耐受性测试,统计高致死溶解氧水平下幼虾的存活性状,分析不同品系间及同一品系不同家系间耐低氧性状的差异,评估其遗传参数;对耐受性有显著差异的家系个体的鳃、肌肉、肝胰腺组织进行组织学观察,并比较其细胞水平上的差异。结果显示,在0.3mg/L低氧条件下,高抗品系和快大品系的家系半致死存活率SS50均表现出显著差异(P2.52%;家系间存活时间的变异系数分别为60%和45%。利用阈值性状动物模型,以半致死时的个体存活状态作为观测值(存活为1,死亡为0)估算的遗传参数为(0.345±0.031)~(0.378±0.029),经过转换后在连续变化的观测值尺度上的遗传力为(0.219±0.031)~(0.237±0.029),表现为中等遗传力水平。高抗品系中低氧耐受家系和低氧敏感家系的鳃、肌肉和肝胰腺组织均出现不同程度的损伤,但不同的家系间表现出不同程度的抗逆生理特征差异,这可能与高抗不同家系抗逆耐受力强弱有关。不同家系间在低氧胁迫下的存活时间、生理特性差异以及各品系遗传力估算结果表明,凡纳滨对虾对高致死溶解氧水平的耐受性状存在丰富的遗传变异,具有选育的可行性。

关键词：凡纳滨对虾低溶解氧遗传力组织损伤选育

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水产养殖环境中农兽药物的污染暴露水平及其风险影响评价

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环境科学 2024年第1期45卷 151-158页

作者：张楷文张海燕孔聪顾润润田良良杨光昕王媛陈冈沈晓盛中国水产科学研究院东海水产研究所上海200090 上海海洋大学食品学院上海201306 上海市金山区水产技术推广站上海201599

为探究水产养殖环境中农兽药物污染暴露水平及其对生态环境的风险影响,以上海地区水产养殖环境为例,于2022年7~9月采集40家主要水产养殖场中水体、底泥以及投入品,采用超高压液相色谱-静电场轨道离子阱质谱法分析其农兽药物的含量及种类... 详细信息

为探究水产养殖环境中农兽药物污染暴露水平及其对生态环境的风险影响,以上海地区水产养殖环境为例,于2022年7~9月采集40家主要水产养殖场中水体、底泥以及投入品,采用超高压液相色谱-静电场轨道离子阱质谱法分析其农兽药物的含量及种类,并通过风险商值(RQ)法对底泥和水体中的农兽药物污染进行生态风险评估.结果表明,204份样品(水样72份、泥样72份和投入品60份)中共筛出13种药物,分别是扑草净、多菌灵、西草净、扑灭津、氟苯尼考、西玛津、金刚烷胺、地西泮、甲氧苄啶、环丙沙星、氧氟沙星、甲苯咪唑和恩诺沙星,其中水样中12种,浓度在0.016~2.084μg·L^(-1)之间.泥样中7种,含量在0.018~23.101μg·kg^(-1)之间.投入品中4种,含量在1.979~101.940μg·kg^(-1)之间.水体与底泥同时发现的药物有7种.风险商值评价结果显示,农兽药残留在水产养殖场底泥和水体中均存在高中风险,其中生态风险最高的药物为多菌灵,RQ值分别为3.848和1.580,表现为高风险.建议加强养殖环境中农兽药物外源进入的控制与管理,以保护水产养殖环境的生态系统健康.

关键词：养殖环境农兽药暴露水平风险评估上海地区

Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping

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Acta Oceanologica Sinica 2006年第5期25卷 151-156,157页

作者： DONG Shirui kong jie ZHANG Qingwem LIU Ping MENG Xianhong wang Rucai College of Life sciences and Technology Ocean University of China Qingdao 266003 China Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine fisheries Resources Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China

Two microsateUite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci （locus RS0622 and locus EN0033 ） by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0. 024 1 and 0. 493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content （PIC） was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F1 and three F2 generations were determined by matching the genotype at each locus.

关键词： Fenneropenaeus chinensis microsateUite pedigree genotype

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基于Bayes方法的东海小黄鱼资源评析

上海海洋大学学报 2011年第6期20卷 873-882页

作者：李九奇叶昌臣王文波尹增强陈绍军大连海洋大学海洋工程学院辽宁大连116023 辽宁省海洋水产科学研究院辽宁大连116023

利用东海区渔业资源动态监测资料、采用基于Bayes方法的Pella-Tomlinson模型对东海区小黄鱼(Pseudosciaenapolyactis)渔业资源动态进行了科学评析,同时,在不同渔业政策选择下,对2004-2020年的资源量与渔获量进行了预测模拟研究。研究结... 详细信息

利用东海区渔业资源动态监测资料、采用基于Bayes方法的Pella-Tomlinson模型对东海区小黄鱼(Pseudosciaenapolyactis)渔业资源动态进行了科学评析,同时,在不同渔业政策选择下,对2004-2020年的资源量与渔获量进行了预测模拟研究。研究结果显示:东海区小黄鱼渔业资源的环境容纳量K为3.4 514 t;内禀增长率r为1.146;1991年的资源生物量为338 493 t、尔后持续上升到1994年的370 923 t。1994年以后又持续下降,到2003年下降到最低值111 253 t;最大持续产量(Maximum sustainable yield,MSY)为107 806 t左右;支持MSY所需的渔业资源生物量为197 503 t;支持MSY所需的捕捞努力量为441 097 6网次;当前资源量与环境容纳量K的比值为0.282,即当前资源量已不足原始资源量的30%;若2004年以后使用0.4的捕捞死亡系数,该渔业资源可以实现持续最优利用。

关键词：小黄鱼 Bayes 东海渔业资源评估种群动态模拟