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Association between Exposure of Rare Earth Elements and Outcomes of In Vitro Fertilization-Embryo Transfer in Beijing

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Biomedical and Environmental Sciences 2024年第8期37卷 876-886页

作者： Yutong Wang Jing Li Shirong Xu Shengli Lin Zhenchen Hou Linlin Wang Yali Huang Yue Sun Wei Guo Lailai Yan Ying Wang chan tian State Key Laboratory of Female Fertility Promotion Center for Reproductive MedicineDepartment of Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Department of Medical Genetics Peking UniversityBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China Central Laboratory of School of Public Health Peking UniversityBeijing 100191China School of Basic Medical Sciences Peking UniversityBeijing 100191China

Objective The study aimed to investigate the impact of rare earth elements(REEs)exposure on pregnancy outcomes of in vitro fertilization-embryo transfer(IVF-ET)by analyzing samples from *** A total of 141 couples were *** and follicular fluid from the wives and semen plasma from the husbands,were analyzed for REEs using inductively coupled plasma mass spectrometry(ICP-MS).Spearman's correlation coefficients and the Mann–Whitney U test were used to assess correlations and compare REE concentrations among three types of samples,*** models were utilized to estimate the individual REE effect on IVF-ET outcomes,while BKMR and WQS models explored the mixture of REE interaction effects on IVF-ET *** Higher La concentration in semen(median 0.089 ng/mL,P=0.03)was associated with a lower fertilization ***,this effect was not observed after artificial selection intervention through intracytoplasmic sperm injection(ICSI)(P=0.27).In semen,the REEs mixture did not exhibit any significant association with clinical *** Our study revealed a potential association between high La exposure in semen and a decline in fertilization rate,but not clinical pregnancy *** is the first to report REEs concentrations in follicular fluid with La,Ce,Pr,and Nd found at significantly lower concentrations than in serum,suggesting that these four REEs may not accumulate in the female reproductive ***,at the current exposure levels,mixed REEs exposure did not exhibit reproductive toxicity.

关键词： Rare earth elements In vitro fertilization Pregnancy outcomes Mixture exposure analysis

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建筑工程招投标阶段的造价控制管理

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建筑与装饰 2024年第11期 40-42页

作者：刘邦哲刘天婵银座集团股份有限公司山东济南 250000 山东国泰实业有限公司山东济南 250000

近年来,我国建筑建设投资规模和总里程不断扩大,有利带动了社会经济发展.随着技术的发展,参与建筑建设的施工企业已不局限于追求生产经营规模,而更着重于企业发展的高质量.因此,施工企业应对工程成本开展全面分析,科学决策,实施精准控制... 详细信息

近年来,我国建筑建设投资规模和总里程不断扩大,有利带动了社会经济发展.随着技术的发展,参与建筑建设的施工企业已不局限于追求生产经营规模,而更着重于企业发展的高质量.因此,施工企业应对工程成本开展全面分析,科学决策,实施精准控制.本文深入研究建筑工程招投标阶段的造价控制问题,并提出建筑工程招投标阶段的造价控制管理策略.

关键词：建筑工程招投标造价控制管理

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Ultrastable nickel single-atom catalysts with high activity and selectivity for electrocatalytic CO_(2)methanation

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Nano Research 2023年第7期16卷 8987-8995页

作者： Ling-chan tian Jin-Nian Hu Yang Meng Jin-Xia Liang Chun Zhu Jun Li School of Chemistry and Chemical Engineering Guizhou UniversityGuiyang 550025China Department of Chemistry and Guangdong Provincial Key Laboratory of Catalytic Chemistry Southern University of Science and TechnologyShenzhen 518055China Department of Chemistry and Engineering Research Center of Advanced Rare-Earth Materials of Ministry of Education Tsinghua UniversityBeijing 100084China

Electrochemical conversion of CO_(2)into valuable hydrocarbon fuel is one of the key steps in solving carbon emission and energy ***,we report a non-noble metal catalyst,nickel single-atom catalyst(SAC)of Ni_(1)/UiO-66-NH_(2),with high stability and selectivity for electrochemical reduction of CO_(2)to CH_(4).Based on ab initio molecular dynamics(AIMD)simulations,the CO_(2)molecule is at first reduced into CO_(2)-when stably adsorbed on a Ni single atom with the bidentate coordination *** evaluate its activity and selectivity for electrocatalytic reduction of CO_(2)to different products(HCOOH,CO,CH3OH,and CH_(4))on Ni_(1)/UiO-66-NH_(2),we have used density functional theory(DFT)to study different reaction *** results show that CH_(4) is generated preferentially on Ni_(1)/UiO-66-NH_(2)and the calculated limiting potential is as low as-0.24 ***,the competitive hydrogen evolution reaction is unfavorable at the activation site of Ni_(1)/UiO-66-NH_(2)owing to the higher limiting potential of-0.56 ***,the change of Ni single atom valence state plays an important role in promoting CO_(2)reduction to CH_(4).This work provides a theoretical foundation for further experimental studies and practical applications of metal-organic framework(UiO-66)-based SAC electrocatalysts with high activity and selectivity for the CO_(2)reduction reaction.

关键词： single atom catalyst density functional theory CO_(2)reduction metal-organic framework electrocatalysis

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Single-copy Loss of Rho Guanine Nucleotide Exchange Factor 10(arhgef10)Causes Locomotor Abnormalities in Zebrafish Larvae

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Biomedical and Environmental Sciences 2022年第1期35卷 35-44页

作者： ZHANG Yi AN Ming Xing GONG Chen LI Yang Yang WANG Yu Tong LIN Meng LI Rong tian chan Department of Obstetrics and Gynecology Center for Medical GeneticsSchool of Basic Medical SciencesThird HospitalPeking UniversityBeijing 100191China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Department of Medical Genetics Center for Medical GeneticsPeking University Health Science CenterBeijing 100191China

Objective To determine if ARHGEF10 has a haploinsufficient effect and provide evidence to evaluate the severity,if any,during prenatal *** Zebrafish was used as a model for generating *** pattern of arhgef10 expression in the early stages of zebrafish development was observed using whole-mount in situ hybridization(WISH).CRISPR/Cas9 was applied to generate a zebrafish model with a single-copy or homozygous arhgef10 *** and light/dark tests were performed in arhgef10^(−/−),arhgef10^(+/−),and wild-type zebrafish ***10 was knocked down using small interferon RNA(siRNA)in the SH-SY5Y cell line,and cell proliferation and apoptosis were determined using the CCK-8 assay and Annexin V/PI staining,*** WISH showed that during zebrafish embryonic development arhgef10 was expressed in the midbrain and hindbrain at 36-72 h post-fertilization(hpf)and in the hemopoietic system at 36-48 *** zebrafish larvae with single-copy and homozygous arhgef10 deletions had lower exercise capacity and poorer responses to environmental changes compared to wild-type zebrafish ***,arhgef10^(−/−) zebrafish had more severe symptoms than arhgef10^(+/-) *** of ARHGEF10 in human neuroblastoma cells led to decreased cell proliferation and increased cell *** Based on our findings,ARHGEF10 appeared to have a haploinsufficiency effect.

关键词： arhgef10 Zebrafish CRISPR/Cas9 Haploinsufficiency Copy loss

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The First Chinese Case of Creutzfeldt-Jakob Disease with Mutation of E200K in PRNP

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Biomedical and Environmental Sciences 2010年第2期23卷 158-160页

作者： CHEN GAO QI SHI WEI ZHOU chan tian HHUI-YING JIANG BAO-YUN ZHANG JUN HAN XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To investigate epidemiological, clinical and genetic features of the first Chinese case of Creutzfeldt-Jakob disease （CJD） with mutation of E2OOK in PRNP. Methods The general epidemiological and clinical data were collected; CSF 14-3-3 protein was analyzed by Western blot; The PRNP was amplified by PCR and analyzed. Results A missense mutation in codon 200 （E200K） of the PRNP was identified in this patient; CSF 14-3-3 protein was positive; sleep disturbance was the initial sign and the other symptoms gradually appeared, including memory loss, dizziness and ataxia. Conclusion The CJD patient who was first reported in China has a missense mutation in codon 200 （E200K） of the PRNP, and the codon 129 is a methionine homozygous genotype.

关键词： CJD PRNP Sleep disturbance 14-3-3

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Establishment of a Stable PrP^(Sc) Panel from Brain Tissues of Experimental Hamsters with Scrapie Strain 263K

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Biomedical and Environmental Sciences 2009年第2期22卷 151-156页

作者： BAO-YUN ZHANG chan tian JUN HAN CHEN GAO QI SHI JIAN-MING CHEN HUI-YING JIANG WEI ZHOU AND XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Objective To establish a stable PrP^Sc panel from brain tissues of experimental hamsters infected with scrapie agent 263K for evaluating diagnostic techniques of human and animals＇ prion diseases. Methods Thirty brain tissue samples from hamsters intracerebrally infected with scrapie strain 263K and another 30 samples from normal hamsters were selected to prepare 10%, 1%, and 0.5% brain homogenates, which were aliquoted into stocks. PrP^Sc in each brain homogenate was determined by proteinase K digestions followed by Western blot assay and partially by immunohistochemistry. Stability and glycoforms of PrP^Sc were repeatedly detected by prp^SC-specific Western blots in half a year and 3 years later. Results PrPsc signals were observed in all 10% brain homogenates of infected hamsters. Twenty out of 30 stocks and 19 out of 30 stocks were PrPsc positive in 1% and 0.5% brain homogenatesof infected hamsters, respectively. TWenty-seven out of 30 stocks presented three positive bands in 10% brain homogenates, whereas none of 1% and 0.5% homogenates contained 3 bands. The detection of prpSc-specific signals stored in half a year and 3 years later demonstrated that the ratio of PrPsc positive samples and glycoforms was almost unchanged. All normal hamsters＇ brain homogenates were PrP^Sc negative. Conclusion A PrP^Sc panel of prion disease can be established, which displays reliably stable prp^Sc-specific signals and glycoforms.

关键词： TSE prp^Sc Panel Glycoforms Stability

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Characterization of the pheromone receptors in Mythimna loreyi reveals the differentiation of sex pheromone recognition in Mythimna species

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Insect Science 2024年第1期31卷 173-185页

作者： chan Wang Lei Liu tian-Yu Huang Yu Zhang Yang Liu Gui-Rong Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina Guangdong Laboratory of Lingnan Modern Agriculture ShenzhenGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina School of Forestry Northeast Forestry UniversityHarbinChina Key Laboratory of Biohazard Monitoring Green Prevention and Control for Artificial GrasslandMinistry of Agriculture and Rural AffairsInstitute of Grassland Research of Chinese Academy of Agricultural SciencesInner Mongolia HohhotChina

Pheromone receptors(PRs)are key proteins in the molecular mechanism of pheromone recognition,and exploring the functional differentiation of PRs between closely related species helps to understand the evolution of moth mating *** components of the agricultural pest Mythimna loreyi have turned into(Z)-9-tetradecen-1-yl acetate(Z9-14:OAc),(Z)-7-dodecen-1-yl acetate(Z7-12:OAc),and(Z)-11-hexadecen-1-yl acetate,while the composition differs from that of *** in the genus *** understand the molecular mechanism of pheromone recognition,we sequenced and analyzed antennal transcriptomes to identify 62 odorant receptor(OR)*** expression levels of all putative ORs were analyzed using differentially expressed gene *** candidate PRs were quantified and functionally characterized in the Xenopus oocytes ***6 and MlorPR3 were determined to be the receptors of major and minor components Z9-14:OAc and Z7-12:***1 and female antennae(FA)-biased MlorPR5 both possessed the ability to detect pheromones of sympatric species,including(Z,E)-9,12-tetradecadien-1-ol,(Z)-9-tetradecen-1-ol,and(Z)-*** on the comparison of PR functions between *** and ***,we analyzed the differentiation of pheromone recognition mechanisms during the evolution of the mating systems of 2 Mythimna species.

关键词： antennal transcriptome Mythimna loreyi pheromone receptor sex pheromone Xenopus oocyte

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The effect of an early oral stimulation program on oral feeding of preterm infants

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International Journal of Nursing Sciences 2014年第1期1卷 42-47页

作者： tian-chan Lyu Yu-xia Zhang Xiao-jing Hu Yun Cao Ping Ren Yue-jue Wang School of Nursing Fudan UniversityShanghaiPR China Children’s Hospital of Fudan University ShanghaiPR China

Purpose:To evaluate the effect of an oral stimulation program on preterm ***:Preterm infants(n=72)were randomly assigned to experimental and control ***(n=36)received routine care while the experimental group(n=36)received oral stimulation in addition to routine *** age,total intake volume,body weight,the transition time from initiation of oral feeding to full oral feeding and feeding efficiency were ***:Postmenstrual age and full oral feeding weight were significantly lower in the experimental group(p<0.05).The time from initiation of oral feeding to full oral feeding was significantly shorter in the experimental group(p<0.05)while feeding efficiency was higher in the experimental group(p<0.05)compared to *** significant differences existed in hospital stay length or weight gain ***:An early oral stimulation program is beneficial in preterm infants.

关键词： Infant Infant nutrition Premature Weight gain

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Evidence of compliance with and effectiveness of guidelines for noninvasive prenatal testing in China:a retrospective study of 189,809 cases

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Science China(Life Sciences) 2020年第3期63卷 319-328页

作者： chan tian Tao Deng Xiuhuang Zhu Chen Gong Yangyu Zhao Yuan Wei Rong Li Xiufeng Xu Miaonan He Zhiwei Zhang Jing Cheng Mol BenWillem Jie Qiao Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China Beijing CapitalBio Medical Laboratory Beijing 101111China Department of Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China National Engineering Research Center for Beijing Biochip Technology Beijing 102206China Department of Biomedical Engineering Tsinghua University School of MedicineBeijing 100084China Robinson Institute School of Paediatrics and Reproductive HealthUniversity of AdelaideSAAustralia Beijing Advanced Innovation Center for Genomic Beijing 100871China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China

In China,the medical guidelines recommend performing noninvasive prenatal testing(NIPT)with caution for pregnant women aged 35 years or ***,the Mother and Child Health Care Law suggests that all primiparous women whose age is older than 35 years undergo prenatal *** two inconsistent suggestions/recommendations have made obstetricians confused about whether to offer NIPT to these older pregnant *** face this issue and find out the solution we performed a retrospective study of 189,809 NIPT samples collected from 28 provincial-leveled administrative units in *** 1,564 women with high-risk pregnancies who underwent NIPT,459(29.3%)did not participate in *** compound sensitivity and specificity of NIPT for trisomies 21,18 and 13 detection was 99.1%(95%CI,98.0%–99.6%)and 99.9%(95%CI,98.8%–99.9%),*** secundiparous women,NIPT showed high sensitivity and specificity similar to that in primiparous *** observed risk for trisomies 21 and 18 significantly increased when the maternal age was 39 and *** the publication of the current NIPT policy,the follow-up rate at our center was 97.9%;however,a large number of women are not in maternal and infant care networks nationwide,and that makes the follow-up rate outside our center relatively *** study shows that to balance the prevention of major aneuploidies and the limited resources for prenatal diagnosis,the cut-off age of 35 for invasive prenatal diagnosis might be *** the NIPT guidelines are well written,how to practice it effectively,especially in less industrialized areas,is worth discussing.

关键词： NIPT risk for trisomy NIPT policy in China prenatal diagnosis maternal age

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Direct detection of C9orf72 hexanucleotide repeat expansions by nanopore biosensor

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Chinese Chemical Letters 2024年第5期35卷 351-354页

作者： Xinqiong Li Guocheng Rao Xi Peng chan Yang Yanjing Zhang Yan tian Xianghui Fu Jia Geng State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Division of Endocrinology and Metabolism West China School of Medicine/West China HospitalSichuan UniversityChengdu 610041China Department of Endocrinology and Metabolism Affiliated Hospital of North Sichuan Medical CollegeNorth Sichuan Medical CollegeNanchong 637000China Division of Endocrinology and Metabolism National Clinical Research Center for GeriatricsState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Department of Laboratory Medicine State Key Laboratory of BiotherapyMed-X Center for ManufacturingWest China HospitalSichuan University and Collaborative Innovation CenterChengdu 610041China tianfu Jincheng Laboratory City of Future MedicineChengdu 641400China

Diagnostic C9orf72 hexanucleotide repeat expansions(C9-HRE)is essential for the early and accurate diagnosis of amyotrophic lateral sclerosis(ALS)and will provide support for the prognosis and gene therapy of *** the present study,by combining catalytic hairpin assembly(CHA)with Mycobacterium smegmatis porin A(MspA)nanopore,a new nanopore-based strategy for the detection of C9-HRE was *** than 30 repeats of C9-HRE could be detected via this method,and the results have the potential to help distinguish between patients and healthy ***,the method demonstrated its great specificity for C9-HRE by identifying other repeat *** the high selectivity,this approach had been successfully used to detect C9-HRE in cell and blood samples with high *** detection strategy is user-friendly and has a strong anti-interference ability,thus providing a powerful tool for clinical diagnosis.

关键词： MspA nanopore C9orf72 HRE Detection Biosensor Catalytic hairpin assembly

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