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Determination of ribose and phosphorus contents in Haemophilus influenzae type b capsular polysaccharide by a quantitative NMR method using a single internal standard

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Chinese Journal of Natural Medicines 2022年第8期20卷 633-640页

作者： QIN Chun-Jun HU Jing TONG Wei ZHANG Teng-Teng tian guang-zong ZoU Xiao-Peng LIU Jian-Kai YIN Jian Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China National&Local Joint Engineering Laboratory for Novel Vaccine Development Technology Beijing Minhai Biotechnology Co.Ltd.Beijing 102600China Jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines Jiangnan UniversityWuxi 214122China

The ribose and phosphorus contents in Haemophilus influenzae type b(Hib)capsular polysaccharide(CPS)are two important chemical indexes for the development and quality control of Hib conjugate vaccine.A quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard was developed for simultaneous determination of ribose and phosphorus contents in Hib ***(HMPA)was successfully utilized as an internal standard in quantitative ^(1)H-NMR method for ribose content *** ribose and phosphorus contents were found to be affected by the concentration of polysaccharide ***,15–20 mg·L^(−1) was the optimal concentration range of Hib CPS in D_(2)O solution for determination of ribose and phosphorus contents by this *** ribose and phosphorus contents obtained by the quantitative NMR were consistent with those obtained by traditional chemical *** conclusion,this quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard shows good specificity,accuracy and precision,providing a valuable approach for the quality control of Hib glycoconjugate vaccines.

关键词： Haemophilus influenzae type b Capsular polysaccharide Quantitative^(1)H-NMR Quantitative^(31)P-NMR Ribose content Phosphorus content

Total synthesis of D-glycero-D-mannno-heptose 1β, 7-bisphosphate with 3-O-amyl amine linker and its monophosphate derivative

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Chinese Journal of Natural Medicines 2020年第8期18卷 628-632页

作者： ZOU Xiao-Peng QIN Chun-Jun HU Jing FU Jun-Jie tian guang-zong MOSCOVITZ Oren SEEBERGER Peter H. YIN Jian Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Department of Biomolecular Systems Max Planck Institute of Colloids and InterfacesPotsdam 14476Germany Wuxi School of Medicine Jiangnan UniversityWuxi 214122China

D-Glycero-D-mannno-heptose 1β,7-bisphosphate(HBPβ)is an important intermediate for constructing the core structure of Gram-negative bacterial lipopolysaccharides and was reported as a pathogen-associated molecular pattern(PAMP)that regulates immune ***βwith 3-O-amyl amine linker and its monophosphate derivative D-glycero-D-mannno-heptose 7-phosphate(HP)with 1α-amyl amine linker have been synthesized as candidates for immunity study of HBPβ.The O3-amyl amine linker of heptose was installed by dibutyltin oxide-mediated regioselective alkylation under fine-tuned protecting *** stereoselective installation of 1β-phosphate ester was achieved by NIS-mediated phosphorylation at low *** strategy for installation of 3-O-amyl amine linker onto HBP derivative can be expanded to the syntheses of other conjugation-ready carbohydrates bearing anomeric phosphoester.

关键词： HBPβ-3-O-amyl amine HP-1α-O-amyl amine 3-O-Alkylation NIS-mediated phosphorylation

Chemical synthesis of a synthetically useful L-galactosaminuronic acid building block

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Chinese Journal of Natural Medicines 2022年第5期20卷 387-392页

作者： QIN Chun-Jun HOU Hong-Li DING Mei-Ru QI Yi-Kuan tian guang-zong ZOU Xiao-Peng FU Jun-Jie HU Jing YIN Jian Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Wuxi School of Medicine Jiangnan UniversityWuxi 214122China

Most bacterial cell surface glycans are structurally unique, and have been considered as ideal target molecules for the developments of detection and diagnosis techniques, as well as vaccines. Chemical synthesis has been a promising approach to prepare well-defined oligosaccharides, facilitating the structure-activity relationship exploration and biomedical applications of bacterial glycans. L-Galactosaminuronic acid is a rare sugar that has been only found in cell surface glycans of gram-negative bacteria. Here, an orthogonally protected L-galactosaminuronic acid building block was designed and chemically synthesized. A synthetic strategy based on glycal addition and TEMPO/BAIB-mediated C6 oxidation served well for the transformation of commercial L-galactose to the corresponding L-galactosaminuronic acid. Notably, the C6 oxidation of the allyl glycoside was more efficient than that of the selenoglycoside. In addition, a balance between the formation of allyl glycoside and the recovery of selenoglycoside was essential to improve efficiency of the NIS/TfOH-catalyzed allylation. This synthetically useful L-galactosaminuronic acid building block will provide a basis for the syntheses of complex bacterial glycans.

关键词： L-galactosaminuronic acid Chemical synthesis Orthogonal protection Glycal addition C6 oxidation

固定化T1酶合成聚酯增塑剂及其应用性能的研究

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塑料工业 2021年第10期49卷 137-142,153页

作者：李冉刘红霞田光宗尹健夏小乐江南大学工业生物技术教育部重点实验室江苏无锡214122 江南大学生物工程学院江苏无锡214122

以三羟甲基丙烷(TMP)、二羟甲基丁酸(DMBA)和丁酸为原料,以固定化T1酶为催化剂,采用酯化缩聚法,优化酶法合成工艺,通过红外光谱和凝胶渗透色谱对聚酯产物进行了表征,并对聚酯增塑剂-聚氯乙烯(PVC)共混膜的增塑效率、热稳定性和迁移性进... 详细信息

以三羟甲基丙烷(TMP)、二羟甲基丁酸(DMBA)和丁酸为原料,以固定化T1酶为催化剂,采用酯化缩聚法,优化酶法合成工艺,通过红外光谱和凝胶渗透色谱对聚酯产物进行了表征,并对聚酯增塑剂-聚氯乙烯(PVC)共混膜的增塑效率、热稳定性和迁移性进行了研究。结果表明,物质的量比为1∶9的TMP/DBMA酯化合成的聚酯产物,其玻璃化转变温度和断裂伸长率方面对PVC共混膜的增塑效率相当于邻苯二甲酸二辛酯(DOP)的72.84%和87.10%。与DOP-PVC共混膜相比,聚酯增塑剂-PVC共混膜具有更好的热稳定性,且其在乙醇和正己烷中的迁移率分别为DOP的47.53%和14.71%,综合展现出了良好的增塑性能和优异的耐迁移性。

关键词：固定化T1脂肪酶聚酯增塑剂增塑性能

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新型葡萄糖型-寡聚糖氨基酸的合成

食品与发酵工业 2016年第12期42卷 68-72页

作者：王兆亚王晓丽田光宗尹健江南大学生物工程学院江苏无锡214122

糖氨基酸(sugar amino acids,SAAs)是一类广泛存在于自然界中的同时具有羧基和氨基的多功能骨架分子,其末端功能性基团的多样性为研究者提供了构建结构多样性天然类似物的可能。该文以一种简单、高效的方法合成了新型葡萄糖型-寡聚糖氨... 详细信息

糖氨基酸(sugar amino acids,SAAs)是一类广泛存在于自然界中的同时具有羧基和氨基的多功能骨架分子,其末端功能性基团的多样性为研究者提供了构建结构多样性天然类似物的可能。该文以一种简单、高效的方法合成了新型葡萄糖型-寡聚糖氨基酸。首先从葡萄糖型糖氨基酸1为起始原料,通过选择性正交保护氨基和羧基,合成了2种糖氨基酸砌块2和3。然后,利用液相肽偶联方法合成了以酰胺键连接的新型葡萄糖型-二聚糖氨基酸OSAA-1(产率67%)、三聚糖氨基酸OSAA-2(产率59%)和四聚糖氨基酸OSAA-3(产率46%)。所有合成的新化合物均经过IR、1H NMR、13C NMR和HRMS的表征。化学方法合成的寡聚糖氨基酸,是一种短肽模拟物,可用于制备纯度较高、稳定性较好的短链保健型肽制品。此外,经过调整合成策略可以合成抗氧化类的短肽,可作为功能性食品添加剂的潜在模拟物。

关键词：葡萄糖寡聚糖氨基酸肽偶联法保健肽制品食品添加剂

Research status and prospects of the fractal analysis of metal material surfaces and interfaces

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 20-38页

作者： Qinjin Dai Xuefeng Liu Xin Ma Shaojie tian Qinghe Cui Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China Beijing Laboratory of Metallic Materials and Processing for Modern Transportation University of Science and Technology BeijingBeijing 100083China Laboratory for Advanced Materials Processing of Ministry of Education University of Science and Technology BeijingBeijing 100083China School of Mechanical and Electrical Engineering Xinjiang Institute of EngineeringUrumqi 830023China

As a mathematical analysis method,fractal analysis can be used to quantitatively describe irregular shapes with self-similar or self-affine *** analysis has been used to characterize the shapes of metal materials at various scales and *** methods make it difficult to quantitatively describe the relationship between the regular characteristics and properties of metal material surfaces and ***,fractal analysis can be used to quantitatively describe the shape characteristics of metal materials and to establish the quantitative relationships between the shape characteristics and various properties of metal *** the perspective of two-dimensional planes and three-dimensional curved surfaces,this paper reviews the current research status of the fractal analysis of metal precipitate interfaces,metal grain boundary interfaces,metal-deposited film surfaces,metal fracture surfaces,metal machined surfaces,and metal wear *** relationship between the fractal dimensions and properties of metal material surfaces and interfaces is *** from three perspectives of fractal analysis,namely,research scope,image acquisition methods,and calculation methods,this paper identifies the direction of research on fractal analysis of metal material surfaces and interfaces that need to be *** is believed that revealing the deep influence mechanism between the fractal dimensions and properties of metal material surfaces and interfaces will be the key research direction of the fractal analysis of metal materials in the future.

关键词： metal material surfaces and interfaces fractal analysis fractal dimension homogeneity

Review of precipitation strengthening in ultrahigh-strength martensitic steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 256-269页

作者： Zhihao tian Chunlei Shang Chaolei Zhang Xiaoye Zhou Honghui Wu Shuize Wang Guilin Wu Junheng Gao Jiaming Zhu Xinping Mao Institute for Carbon Neutrality University of Science and Technology BeijingBeijing 100083China Institute of Steel Sustainable Technology Liaoning Academy of MaterialsShenyang 110004China Department of Materials Science and Engineering Shenzhen MSU-BIT UniversityShenzhen 518172China Institute of Materials Intelligent Technology Liaoning Academy of MaterialsShenyang 110004China School of Civil Engineering Shandong UniversityJinan 250061China

Martensite is an important microstructure in ultrahigh-strength steels,and enhancing the strength of martensitic steels often involves the introduction of precipitated phases within the martensitic *** considerable research efforts devoted to this area,a systematic summary of these advancements is *** review focuses on the precipitates prevalent in ultrahigh-strength martensitic steel,primarily carbides(e.g.,MC,M_(2)C,and M_(3)C)and intermetallic compounds(e.g.,Ni Al,Ni_(3)X,and Fe_(2)Mo).The precipitation-strengthening effect of these precipitates on ultrahigh-strength martensitic steel is discussed from the aspects of heat treatment processes,microstructure of precipitate-strengthened martensite matrix,and mechanical ***,a perspective on the development of precipitation-strengthened martensitic steel is presented to contribute to the advancement of ultrahigh-strength martensitic *** review highlights significant findings,ongoing challenges,and opportunities in the development of ultrahigh-strength martensitic steel.

关键词： ultrahigh-strength martensitic steel precipitation strengthening mechanical property carbide intermetallic compound

Nanocarrier-mediated siRNA delivery:a new approach for the treatment of traumatic brain injury-related Alzheimer's disease

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Neural Regeneration Research 2025年第9期20卷 2538-2555页

作者： Jie Jin Huajing Zhang Qianying Lu Linqiang tian Sanqiao Yao Feng Lai Yangfan Liang Chuanchuan Liu Yujia Lu Sijia tian Yanmei Zhao Wenjie Ren Institute of Disaster and Emergency Medicine Tianjin UniversityTianjinChina Key Laboratory for Disaster Medicine Technology TianjinChina Henan Medical Key Laboratory for Research of Trauma and Orthopedics The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Clinical Medical Center of Tissue Engineering and Regeneration Xinxiang Medical UniversityXinxiangHenan ProvinceChina Institutes of Health Central Plain Xinxiang Medical UniversityXinxiangHenan ProvinceChina School of Public Health Xinxiang Medical UniversityXinxiangHenan ProvinceChina

Traumatic brain injury and Alzheimer's disease share pathological similarities,including neuronal loss,amyloid-βdeposition,tau hyperphosphorylation,blood-brain barrier dysfunction,neuroinflammation,and cognitive ***,traumatic brain injury can exacerbate Alzheimer's disease-like pathologies,potentially leading to the development of Alzheimer's *** offer a potential solution by facilitating the delive ry of small interfering RNAs across the blood-brain barrier for the targeted silencing of key pathological genes implicated in traumatic brain injury and Alzheimer's disease.U nlike traditional approaches to neuro regeneration,this is a molecula r-targeted strategy,thus avoiding non-specific drug *** review focuses on the use of nanocarrier systems for the efficient and precise delive ry of siRNAs,discussing the advantages,challenges,and future *** principle,siRNAs have the potential to target all genes and non-targetable protein s,holding significant promise for treating various *** the various therapeutic approaches currently available for neurological diseases,siRNA gene silencing can precisely"turn off"the expression of any gene at the genetic level,thus radically inhibiting disease progression;however,a significant challenge lies in delivering siRNAs across the blood-brain *** have received increasing attention as an innovative drug delive ry tool fo r the treatment of brain *** are considered a potential therapeutic strategy with the advantages of being able to cross the blood-brain barrier,targeted drug delivery,enhanced drug stability,and multifunctional *** use of nanoparticles to deliver specific modified siRNAs to the injured brain is gradually being recognized as a feasible and effective *** this strategy is still in the preclinical exploration stage,it is expected to achieve clinical translation in the future,creating a new field of molecular tar

关键词： blood-brain barrier cognitive dysfunction exosomes gene silencing immunotherapy inorganic nanomaterials liposome nanoparticles neuroinflammation polymer nanoparticles stem cells

Optimized interval 2-tuple linguistic aggregation operator based on PGSA and its application in MAGDM

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Journal of Systems Engineering and Electronics 2019年第6期30卷 1192-1201页

作者： zong Mengting SHEN tian CHEN Xi School of Business Nanjing UniversityNanjing 210000China School of Education Nanjing University of Traditional Chinese MedicineNanjing 210000China

This study proposes a multiple attribute group decisionmaking(MAGDM)approach on the basis of the plant growth simulation algorithm(PGSA)and interval 2-tuple weighted average operators for uncertain linguistic weighted aggregation(ULWA).We provide an example for illustration and verification and compare several aggregation operators to indicate the optimality of the assembly *** addition,we present two comparisons to demonstrate the practicality and effectiveness of the proposed *** method can be used not only to aggregate MAGDM problems but also to solve multi-granularity uncertain linguistic *** high reliability,easy programming,and high-speed calculation can improve the efficiency of ULWA ***,the proposed method has the exact characteristics for linguistic information processing and can effectively avoid information distortion and loss.

关键词： multiple attribute group decision making(MAGDM) in terval 2-tuple plant growth simulation algorithm(PGSA) weighted Steiner point.