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Comparison of microscopy and PCR for the detection of human Plasmodium species and Plasmodium knowlesi in southern Myanmar

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 680-685页

作者： thu zar han Kay Thwe han Kyin Hla Aye Thaung Hlaing Kyaw Zin Thant Indra Vythilingam MD Programme Faculty of Medicine&Health SciencesUCSI University Department of Medical Research Lower Myanmar Vector Borne Diseases Control Unit Department of Health Department of Parasitology University Malaya

To determine the distribution of Plasmodium (P) species including Plasmodium knowlesi and to compare the specificity and sensitivity of microscopy with nested PCR in malaria *** study was conducted in Kawthaung, southern Myanmar. Ninety clinically suspected malaria patients were screened for malaria by Giemsa stained microscopy and confirmed by nested *** the participants, 57 (63.3%) were positive and 33 (36.7%) were negative by microscopy. Of positive samples, 39 (68.4%) were Plasmodium falciparum, 17 (29.8%) Plasmodium vivax and 1 (1.8%) Plasmodium malariae, whereas 59-amplified by PCR were 40 (67.8%), 18 (30.5%) and 1 (1.7%) respectively. PCR amplified 2 microscopy negative samples. Two samples of P. falciparum detected by microscopy were amplified as P. vivax and vice versa. All samples were negative for Plasmodium ovale, P. knowlesi and mixed infections. Microscopy had a very good measure of agreement (κ = 0.95) compared to nested PCR. Sensitivity and specificity of microscopy for diagnosis of P. falciparum were 92.5% (95% CI: 79.6-98.4) and 96.0% (95% CI: 86.3-99.5) respectively, whereas for P. vivax were 83.3% (95% CI: 58.6-96.4) and 97.2% (95% CI: 90.3-99.7).ConclusionsP. knowlesi was not detected by both microscopy and PCR. Giemsa stained microscopy can still be applied as primary method for malaria diagnosis and is considered as gold standard. As to the lower sensitivity of microscopy for vivax malaria, those with previous history of malaria and relapse cases should be diagnosed by RDT or PCR combined with microscopy. Inaccuracy of species diagnosis highlighted the requirement of training and refresher courses for microscopists.

关键词： Malaria Plasmodium Plasmodium knowlesi Microscopy

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缅甸大米出口竞争力影响因素的实证分析

缅甸大米出口竞争力影响因素的实证分析

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作者： thu zar NWE 江西财经大学

学位级别：硕士

大米作为主食,是缅甸经济的重要来源,大量外汇收入来自出口。考虑到这一点,本研究旨在衡量缅甸大米出口在国际市场上与亚洲最大的出口国相比的竞争力。这项研究是通过使用2010-2019年贸易面板数据使用显性比较优势（RCA）指数模型的首... 详细信息

大米作为主食,是缅甸经济的重要来源,大量外汇收入来自出口。考虑到这一点,本研究旨在衡量缅甸大米出口在国际市场上与亚洲最大的出口国相比的竞争力。这项研究是通过使用2010-2019年贸易面板数据使用显性比较优势（RCA）指数模型的首次实证尝试。研究结果表明,缅甸大米出口具有很强的竞争和比较优势,通过多元线性回归分析可以明确缅甸大米出口竞争力的99.7%。多元回归分析分为六个因素,诸如年降雨量,耕地,稻田生产,人口,电力获得和贷款因素之类的变量对RCA指数（大米出口）的因变量有正反作用,点上述因变量变化的99.7%。这项研究表明,从长远来看,提高稻米的竞争力是国际稻米市场上具有更大的竞争力,这激发了出口商,各国政府不仅需要着重贷款,而且还要着眼于道路,电力供应,灌溉计划,电信,技术支持等方面的改善。价值链和农业投入和产出市场,以实现令人满意的增长。

关键词：国际竞争力大米出口波特钻石模型 RCA指数

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Different challenges,different approaches and related expenditures of communitybased tuberculosis activities by international non-governmental organizations in Myanmar

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Infectious Diseases of Poverty 2017年第1期6卷 506-513页

作者： Wai Wai han Saw Saw Petros Isaakidis Mohammed Khogali Anthony Reid Nguyen Hoa Ko Ko Zaw Si thu Aung Department of Medical Research Ministry of Health and SportsNo 5Ziwaka RoadDagon Township11191 YangonMyanmar Mddecins sans Frontieres Operational Research UnitLuxembourg cityLuxembourg Centre for Operational Research International Union Against Tuberculosis and Lung DiseaseParisFrance National Tuberculosis Programme Ministry of Health and SportsNay Pyi TawMyanmar National Tuberculosis Programme HanoiVietnam

Background:International non-governmental organizations(INGOs)have been implementing community-based tuberculosis(TB)care(CBTBC)in Myanmar since *** the National TB Programme(NTP)ultimately plans to take over CBTBC,there have been no evaluations of the models of care or of the costs of providing CBTBC in Myanmar by ***:This was a descriptive study using routinely-collected programmatic and financial data from four INGOs during 2013 and 2014,adjusted for *** analysis was performed from the provider *** for sputum examination were not included as it was provided free of charge by *** calculated the average cost per year of each programme and cost per patient completing ***:Four INGOs assisted the NTP by providing CBTBC in areas where access to TB services was *** INGO faced different issues in their contexts and responded with a diversity of *** total costs ranged from US$140754 to US$550221 during the study *** cost per patient completing treatment ranged from US$215 to US$1076 for new cases and US$354 to US$1215 for retreatment cases,depending on the targeted area and the package of services *** INGO appeared less costly,more sustainable and patient oriented than ***:This study revealed a wide variety of models of care and associated costs for implementing CBTBC in diverse and challenging populations and contexts in ***,we recommend a more comprehensive evaluation,including development of a cost model,to estimate the costs of scaling up CBTBC country-wide,and cost-effectiveness studies,to best inform the NTP as it prepares to takeover CBTBC activities from *** awaiting evidence from these studies,model of CBTBC that have higher sustainability potential and allocate more resources to patient-centered care should be given priority support.

关键词： Operational research Cost Sustainability Budget allocation

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低表达衰老标记蛋白30对高钙状态下人晶状体上皮细胞增殖和氧化的影响

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国际眼科杂志 2020年第2期20卷 217-223页

作者：李松蔓韩子豪 Aint thu thu Win 陈曦梁皓广西医科大学第一附属医院眼科

目的:探讨高钙状态下衰老标记蛋白30(SMP30)低表达对人晶状体上皮细胞(LECs)系SRA01/04增殖、氧化应激的影响。方法:设计3条干扰SMP30靶向基因RGN表达的RNAi序列(KD1~3),以空载序列为阴性对照组(NCKD),构建慢病毒载体并感染SRA01/04细胞... 详细信息

目的:探讨高钙状态下衰老标记蛋白30(SMP30)低表达对人晶状体上皮细胞(LECs)系SRA01/04增殖、氧化应激的影响。方法:设计3条干扰SMP30靶向基因RGN表达的RNAi序列(KD1~3),以空载序列为阴性对照组(NCKD),构建慢病毒载体并感染SRA01/04细胞,同时以未感染的SRA01/04细胞作空白对照组(CON)。RT-PCR筛选干扰效率最高的慢病毒载体用于后续实验。采用含15mmol/L CaCl2的完全培养基处理细胞24h模拟发生白内障时人LECs的病理状态,通过BrdU-Elisa法检测细胞增殖活力,并检测细胞超氧化物歧化酶(SOD)活性及氧化型谷胱甘肽/总谷胱甘肽(GSSG/T-GSH)水平以评估细胞氧化应激水平。结果:成功构建KD1~3及NCKD慢病毒载体,感染SRA01/04细胞效率约80%。三种慢病毒载体(KD1~3)敲减效率分别为93%、60%、74%,故选择KD1慢病毒载体进行后续实验。高钙状态下,KD1组细胞相对增殖活力和SOD活力[(2.42±0.08)和(11.69±0.52U/mg)]均低于NCKD组[(2.95±0.08)和(31.10±2.24U/mg)]和CON组[(2.96±0.25)和(26.33±1.04U/mg)],GSSG/T-GSH比值(70.80±2.34)高于NCKD组(15.93±3.47)和CON组(20.05±2.45)(均P0.05)。结论:高钙状态培养下,RGN-RNAi慢病毒载体介导的SMP30低表达SRA01/04细胞增殖活力及抗氧化应激能力减弱,提示SMP30可能具有调控细胞增殖、抗氧化应激的保护作用。

关键词：衰老标记蛋白30 人晶状体上皮细胞高钙状态细胞增殖氧化应激白内障

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高表达衰老标记蛋白30对高钙诱导人晶状体上皮细胞株SRA01/04氧化应激的影响

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中山大学学报（医学版） 2019年第1期40卷 54-61页

作者：李松蔓陈曦韩子豪 thu thu WIN AINT 衷昕唐东永梁皓广西医科大学第一附属医院眼科广西壮族自治区南宁530021

【目的】探讨在高钙诱导细胞氧化应激下,高表达衰老标记蛋白30(SMP30)对人晶状体上皮细胞(HLEC)株SRA01/04增殖活力及抗氧化能力的影响。【方法】实验分3组:SMP30高表达组(OE,实验组)、NCOE组(阴性对照组)及SRA01/04组(空白对照组)。用O... 详细信息

【目的】探讨在高钙诱导细胞氧化应激下,高表达衰老标记蛋白30(SMP30)对人晶状体上皮细胞(HLEC)株SRA01/04增殖活力及抗氧化能力的影响。【方法】实验分3组:SMP30高表达组(OE,实验组)、NCOE组(阴性对照组)及SRA01/04组(空白对照组)。用OE及NCOE慢病毒载体分别转染SRA01/04细胞。15 mmol/LCaCl2处理细胞24 h建立高钙诱导模型。BrdU法检测细胞增殖、超氧化物歧化酶(SOD)、氧化型谷胱甘肽(GSSG)/总谷胱甘肽(T-GSH)法检测细胞氧化指标。【结果】荧光显微镜下可见各组转染细胞有大量绿色荧光蛋白(GFP)表达,转染效率接近80%,提示SMP30高表达SRA01/04细胞模型构建成功。在高钙状态下,OE组细胞相对增殖活力(3.89±0.20)和SOD活力(U/mg,47.5±4.3)均高于NCOE组(2.82±0.34、30.6±4.2)及SRA01/04组(2.96±0.25、26.8±1.5),OE组GSSG/T-GSH比值(2.36±0.51)低于NCOE组(16.36±2.48)及SRA01/04组(20.12±2.54),上述差异均有统计学意义(n=3,P0.05)。【结论】高表达SMP30增加SRA01/04(HLEC)细胞增殖活力、SOD活力及下降GSSG/T-GSH水平,提示SMP30在一定程度上可缓解高钙诱导细胞氧化损伤的进程,可能对HLEC有保护作用。

关键词：衰老标记蛋白30 人晶状体上皮细胞高钙氧化应激超氧化物歧化酶谷胱甘肽细胞增殖

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Folate-targeted nanostructured chitosan/chondroitin sulfate complex carriers for enhanced delivery of bortezomib to colorectal cancer cells

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Asian Journal of Pharmaceutical Sciences 2019年第1期14卷 40-51页

作者： zar Chi Soe Bijay Kumar Poudel hanh thuy Nguyen Raj Kumar Thapa Wenquan Ou Milan Gautam Kishwor Poudel Sung Giu Jin Jee-Heon Jeong Sae Kwang Ku han-Gon Choi Chul Soon Yong Jong Oh Kim College of Pharmacy Yeungnam University Department of Pharmaceutics University of Pharmacy (Yangon) Department of Pharmaceutical Engineering Dankook University College of Korean Medicine Haany University College of Pharmacy Hanyang University

Folate-targeting self-assembled nanoparticles(NPs) using biocompatible and biodegradable natural polymers chitosan(Cs) and chondroitin sulfate(Chs) were developed to address the major challenge in cancer treatment, the selective delivery of nanoparticles to the target site. In this study, we successfully incorporated a hydrophobic drug, bortezomib(Bor), into folic acid(FA)-conjugated Cs/Chs self-assembled NPs(Bor/Cs/Chs-FA) for colorectal cancer therapy. The particle size and polydispersity index of Bor/Cs/Chs-FA were196.5 ± 1.2 nm and0.21 ± 0.5, respectively. A pH-dependent release profile was observed, facilitating cancer cell-targeted drug release under an acidic tumor microenvironment. Moreover, in vitro data revealed enhanced cellular uptake and apoptosis in folate receptor-expressing colorectal cancer cells(HCT-116 and HT-29) as compared to that in lung cancer cells(A549), which do not express folate receptors. Furthermore, intravenous administration of Bor/Cs/Chs-FA in a HCT-116 bearing xenograft mouse model showed that the NPs were a safe and effective drug delivery system. The results suggest that folate-targeted nanoparticle can be effectively applied for efficient chemotherapy of colorectal cancer.

关键词： Bortezomib Chitosan chondroitin sulfate Colorectal cancer Folic acid

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伺服阀前置级流场气穴现象的仿真及试验研究

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机电工程 2014年第10期31卷 1239-1243,1253页

作者：韩笑 Nay zar Aung 曹俊章李松晶哈尔滨工业大学体控制及自动化系黑龙江哈尔滨150001

针对液压阀中的气穴现象会引起液压阀的噪音、性能恶化,甚至导致液压阀失效等问题,对伺服阀前置级喷嘴和挡板之间流场中的气穴现象进行了研究。利用Pro/E软件对流场进行了三维建模,用前处理软件Gambit对三维模型进行了网格划分及边界条... 详细信息

针对液压阀中的气穴现象会引起液压阀的噪音、性能恶化,甚至导致液压阀失效等问题,对伺服阀前置级喷嘴和挡板之间流场中的气穴现象进行了研究。利用Pro/E软件对流场进行了三维建模,用前处理软件Gambit对三维模型进行了网格划分及边界条件设置,利用标准k-ε模型和混合气穴模型建立流场的数学模型,运用FLUENT软件对不同喷嘴入口流速下流场的分布特性及气穴特征进行了仿真分析;同时利用高速摄像机对喷嘴挡板之间的流场分布进行了观测和记录。研究结果表明,在低雷诺数流动条件下,气穴开始在喷嘴外壁和挡板前端的边缘形成;随着雷诺数的增大,在挡板弯曲的附体气穴逐渐长大并随着射流流速的增加出现云状的气穴。将不同流动条件下流场结构和气穴分布的数值计算结果与试验观测结果进行比较,两者基本吻合,说明伺服阀前置级流场气穴仿真模型和数值计算方法是可靠的。

关键词：伺服阀气穴流场喷嘴挡板

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Research to Establish a Set of Combinatorial Indicators to Calculate Flood Vulnerability Index in Thach han-Ben Hai River Basin, Quang Tri Province, Vietnam

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Journal of Environmental Science and Engineering(B) 2021年第4期10卷 138-150页

作者： Ngo Chi Tuan Nguyen Thanh Son Nguyen Kien Dung Can thu Van University of Science Vietnam National UniversityHanoi 1000000Vietnam hanoi University of Natural Resources and Environment Hanoi 1000000Vietnam Ho Chi Minh City University of Natural Resources and Environment Ho Chi Minh City 700000Vietnam

In recent times,approaches to flood prevention and mitigation have changed a *** changes are to shift the focus from flood control to flood risk management,in which the assessment of vulnerability to floods caused by each sector and field corresponding to each flood magnitude is *** flood vulnerability assessment studies are basically holistic based on factors affected by floods or in aspects:economy,society and ecological environment,and aspects that are mentioned in all areas of social *** content of the flood vulnerability assessment method usually considers 3 components:E(Exposure),S(Susceptibility),A(Adaptability-Recovery).Each component will contain many different variables belonging to different objects and different natural-economic-social characteristics in each ***,for each research area,the evaluation method needs to establish an appropriate set of indicators.A set of indicators used to assess flood vulnerability must:be scientific;be practical;simplify the investigation,collection and synthesis of *** study has established a set of combinatorial indicators including 27 indicators for the T-BRB(Thach han-Ben Hai River Basin)on the basis of analysis and synthesis from case studies.

关键词： Vulnerability combine indicators T-BRB

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Simulation of Flood in the Thach han-Ben Hai River Basin(Vietnam)under the Influence of the Upstream Reservoir System

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Journal of Environmental Science and Engineering(B) 2021年第6期10卷 227-236页

作者： Ngo Chi Tuan Can thu Van Nguyen Huu Tuan Nguyen Hai Au hanoi University of Science Vietnam National UniversityHanoiHa Noi 1000000Vietnam Ho Chi Minh City University of Natural Resources and Environment Ho Chi Minh City 700000Vietnam Institute for Environment and Resources Vietnam National UniversityHo Chi Minh CityHo Chi Minh City 700000Vietnam

The central provinces in Vietnam always suffer from the negative impacts of floods every year,especially in the downstream *** Tri province in the TBRB(Thach han-Ben Hai River Basin)is one of the provinces suffering heavy damage caused by floods.A 1-dimensional hydrodynamic model was researched and applied connecting 2 dimensions in the MIKE model set(MIKE FLOOD)to simulate inundation level,inundation time,flood flow velocity for communes in *** results for 111 communes in Quang Tri province show that:39 communes(35%)are not flooded;3 communes are flooded below 0.5 m;15 communes are flooded from 0.5-1.0 m,flooding time is about 1 day;30 communes are flooded from 1.0-2.0 m,inundation time is about 2 days;30 communes are flooded over 2.0 m,flooded for about 3 days,especially 3 communes are flooded over 4.0 *** result helps to develop flood prevention plans for localities in the province.

关键词： Thach han-Ben Hei River basin flood simulation Quang Tri province hydrodynamic model

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急性氧化应激状态下人晶状体上皮细胞株SRA01/04中衰老标记蛋白30、Caspase-3、Ca2+-ATP表达的变化

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广西医科大学学报 2019年第8期36卷 1222-1226页

作者： AINT thu thu WIN 李松蔓韩子豪陈曦唐东永蒋林志梁皓广西医科大学第一附属医院眼科

目的:探讨在急性氧化应激状态下人晶状体上皮细胞株SRA01/04内衰老标记蛋白30(SMP30)、Caspase-3、Ca^2+-ATP表达的变化。方法:通过慢病毒转染建立SMP30过表达(OE)组、过表达空载对照(NC-OE)组、SMP30沉默(KD)组及沉默空载(NC-KD)组SRA0... 详细信息

目的:探讨在急性氧化应激状态下人晶状体上皮细胞株SRA01/04内衰老标记蛋白30(SMP30)、Caspase-3、Ca^2+-ATP表达的变化。方法:通过慢病毒转染建立SMP30过表达(OE)组、过表达空载对照(NC-OE)组、SMP30沉默(KD)组及沉默空载(NC-KD)组SRA01/04细胞,RT-PCR和Western blotting检测SMP30含量鉴定细胞是否转染成功;用300μmol/L H2O2 作用2 h,构建急性氧化应激状态时,PCR检测各组细胞SMP30基因的表达情况,Western blotting检测各组细胞SMP30、Caspase-30和Ca^2+-ATP蛋白的表达量。结果:在正常状态时,OE组SMP30基因及其蛋白表达量高于NC-OE组(P<0.05),KD组的SMP30基因及其蛋白表达量低于NC-KD组(P<0.05)。OE组及KD组细胞SMP30基因表达量均出现增加(P<0.05),与正常状态时相比,急性氧化应激状态时OE组细胞SMP30蛋白表达上调(P<0.05);正常状态时,OE组Caspase-3蛋白表达量低于NC-OE组,KD组Caspase-3蛋白表达量高于NC-KD组(P<0.05)。急性氧化应激状态时,OE组Caspase-3蛋白表达量低于NC-OE组,KD组Caspase-3蛋白表达量低于NC-KD组(P<0.05)。在急性氧化应激状态时OE组Caspase-3蛋白表达量低于正常状态时;KD组的Caspase-3蛋白表达量高于正常状态时KD组(P<0.05)。在正常状态时,OE组Ca^2+-ATP蛋白表达量高于NC-OE组,KD组Ca^2+-ATP蛋白表达量低于NC-KD组(P<0.05)。在急性氧化应激状态时,OE组Ca^2+-ATP蛋白表达量高于NC-OE组,KD组Ca^2+-ATP蛋白表达量低于NC-KD组(P<0.05)。在急性氧化应激状态时OE组的Ca^2+-ATP蛋白表达量高于正常状态时(P<0.05)。结论:在急性氧化应激状态时,SMP30含量出现反应性增加,过表达SMP30可能对人晶状体上皮细胞株SRA01/04具有抗氧化、抗凋亡、增加钙调节活性的作用。

关键词：急性氧化应激人晶状体上皮细胞SRA01/04 衰老标记蛋白30 白内障 Caspase-3 Ca^2+-ATP

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