检索结果-南通市图书馆

Force microscopy of the Caenorhabditis elegans embryonic eggshell

Microsystems & Nanoengineering 2020年第1期6卷 1029-1039页

作者： Roger Krenger Jan T.Burri thomas lehnert Bradley J.Nelson Martin A.M.Gijs Laboratory of Microsystems Ecole Polytechnique Federale de Lausanne1015 LausanneSwitzerland Multi-Scale Robotics Laboratory ETH ZurichZürich 8092Switzerland

Assays focusing on emerging biological phenomena in an animal’s life can be performed during *** the embryo of Caenorhabditis elegans has been extensively studied,its biomechanical properties are largely ***,we demonstrate that cellular force microscopy(CFM),a recently developed technique that combines micro-indentation with high resolution force sensing approaching that of atomic force microscopy,can be successfully applied to *** *** performed,for the first time,a quantitative study of the mechanical properties of the eggshell of living *** embryos and demonstrate the capability of the system to detect alterations of its mechanical parameters and shell defects upon chemical *** addition to investigating natural eggshells,we applied different eggshell treatments,i.e.,exposure to sodium hypochlorite and chitinase solutions,respectively,that selectively modified the multilayer eggshell structure,in order to evaluate the impact of the different layers on the mechanical integrity of the *** element method simulations based on a simple embryo model were used to extract characteristic eggshell parameters from the experimental micro-indentation force-displacement *** found a strong correlation between the severity of the chemical treatment and the rigidity of the ***,our results showed,in contrast to previous assumptions,that short bleach treatments not only selectively remove the outermost vitelline layer of the eggshell,but also significantly degenerate the underlying chitin layer,which is primarily responsible for the mechanical stability of the egg.

关键词： remove rigidity approaching

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive

引用

Microsystems & Nanoengineering 2021年第5期7卷 41-59页

作者： Yang Liu thomas lehnert Martin A.M.Gijs Laboratory of Microsystems Ecole Polytechnique Federale de LausanneCH-1015 LausanneSwitzerland

Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns,appearing as traveling bands with transient locally enhanced cell *** bands are known to be a bacterial chemotactic response to self-generated nutrient gradients during growth in resource-limited *** this work,we studied different parameters of Escherichia coli(***)collective migration,in particular the quantity of bacteria introduced initially in a microfluidic chip(inoculum size)and their exposure to antibiotics(ampicillin,ciprofloxacin,and gentamicin).We developed a hybrid polymer-glass chip with an intermediate optical adhesive layer featuring the microfluidic channel,enabling high-content imaging of the migration dynamics in a single bacterial layer,*** bacteria are confined in a quasi-2D space that is fully observable with a high-magnification microscope ***-chip bacterial motility and traveling band analysis was performed based on individual bacterial trajectories by means of custom-developed *** of swimming speed,tumble bias and effective diffusion properties allowed the assessment of phenotypic heterogeneity,resulting in variations in transient cell density distributions and swimming *** found that incubation of isogeneic£coli with different inoculum sizes eventually generated different swimming phenotype ***,incubation with antimicrobials promoted bacterial chemotaxis in specific cases,despite growth ***,*** filamentation in the presence of antibiotics was assessed,and the impact on motility was *** propose that the observation of traveling bands can be explored as an alternative for fast antimicrobial susceptibility testing.

关键词： traveling collective transient

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dose Efficiency in Dual Source High-Pitch Computed Tomography of the Chest

引用

Advances in Computed Tomography 2014年第4期3卷 51-58页

作者： Boris Bodelle thomas lehnert Martin Beeres thomas Josef Vogl Boris Schulz Department of Diagnostic and Interventional Radiology Clinic of the Goethe University Frankfurt Germany

Objectives: Evaluation of radiation efficiency of dual source high-pitch (DSHP) chest CT in comparison to single source technique with special regards to individual patient anatomy. Methods: 150 consecutive patients who underwent chest CT with automated tube current modulation were evaluated retrospectively and divided into three study groups, each with an equal quantity of 50 patients (DSHP vs. single source 128 slices vs. single source 16 slices). By using a dedicated workstation, volumetric analyses of each of the scanned anatomic area were performed and correlated to the individual dose length product (DLP). The calculated result was defined as dose efficiency. Results: DLP was 203 mGycm (DSHP), vs. 269 mGycm (single source) vs. 273 mGycm (16 slice CT). The total patient volume was lowest in the dual source group with 18956.3 cm3 (vs. 22481.2 cm3 vs. 22133.8 cm3). With regards to the DLP, the calculated dose efficiency of dual source CT was better than the 128 slice CT (p = 0.045) and the 16 slice CT (p < 0.01). Conclusions: DSHP CT has considerably better dose efficiency compared to 16 slice CT. Compared to 128 slice single source technique, the high-pitch mode does not cause any dose penalty when performing chest CT.

关键词： Computed Tomography Lung Radiation Exposure Imaging Dual-Source

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

两例食管癌肉瘤克隆来源的分子生物学分析

引用

中华病理学杂志 2001年第5期30卷 366-366页

作者：孙孟红朱雄增 Peter Moeller Christian Herfarth Magnus von Knebel Doeberitz Hans K Schackert thomas lehnert Johannes Gebert 复旦大学附属肿瘤医院病理科分子病理研究室 Division of Molecular Diagnostic and Therapy University Heidelberg INF 110 69120 Heidelberg Germany

一、材料、方法和结果1.临床病理情况:例1男,56岁.肉眼可见食管中段外生性10.0 cm×4.5 cm肿块,灶性褐色浅表溃疡.切面灰白.组织学见分化差非角化鳞状细胞巢及纤维肉瘤成分混合存在.两种瘤细胞成分均有丰富的核分裂象.分期pT2NOM0,分级... 详细信息

一、材料、方法和结果1.临床病理情况:例1男,56岁.肉眼可见食管中段外生性10.0 cm×4.5 cm肿块,灶性褐色浅表溃疡.切面灰白.组织学见分化差非角化鳞状细胞巢及纤维肉瘤成分混合存在.两种瘤细胞成分均有丰富的核分裂象.分期pT2NOM0,分级Ⅲ.患者术后5个月死于复发.例2男,59岁.食管中1/3段6.6 cm×3.5 cm×2.6 cm息肉状肿块,表面灶性溃疡.切面灰白色,灶性红色.组织学见不同分化程度的鳞状细胞癌.另见网格状分布的基底细胞样癌成分.以上两种成分之间,纤维肉瘤细胞散在分布.分期pT3NOM0,分级Ⅰ～Ⅳ.术后10个月死于肿瘤复发.

关键词：食管癌肉瘤克隆来源分子生物学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：