检索结果-南通市图书馆

Effect of honey on oxidation, chlorination and nitration by purified equine myeloperoxidase

Journal of Coastal Life Medicine 2017年第9期5卷 398-402页

作者： Saad Aissat Hama Benbarek thierry franck Stephan Kohnen Didier Serteyn Moussa Ahmed Ange Mouithys-Mickalad Institute of Veterinary Sciences Ibn Khaldoun UniversityTiaret14000Algeria Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria Faculty of Sciences of Nature and Life Mascara UniversityMascara29000Algeria Centre for Oxygen Research and Development(CORD)Institute of Chemistry B6aUniversity of LiègeSart Tilman4000Liège1Belgium Department of Clinical Sciences Large Animal SurgeryFaculty of Veterinary MedicineB41University of LiègeSart Tilman4000Liège1Belgium Celabor Centre de Services Scientifique et Technique aux EntreprisesZoning Industriel de Petit-RechainAvenue du Parc384650HerveBelgium

Objective: To evaluate the antioxidant effect of honey using two classical methods generally used, and for the first time to test the effect of honey on the oxidation, chlorination and nitration by purified equine myeloperoxidase (MPO). Methods: The antioxidant activity of three Algerian honey samples (nectar honey, mixed honey and honeydew honey) was evaluated by two classical methods, the ferric- reducing/antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity. Results: Honeydew honey had the highest reducing power and DPPH radical-scavenging activity, whereas nectar honey showed the lowest reducing power and DPPH radical-scavenging activity. All honey samples showed a significant inhibitory effect on the chlorination activity of equine MPO, but honeydew honey was the weakest inhibitor. The three samples were poorly inhibitor on the MPO oxidation and nitration activities, except for nectar honey that exerted an inhibitory effect at the highest tested concentration of 10%. These later results seem to contradict those obtained with DPPH and FRAP. Conclusions: The antioxidant capacity of honey is mainly due to the phenolic compounds and flavonoids it contained. It has been suggested that MPO might be involved in the antioxidant, not pro-oxidant, activity of phenolic compounds.

关键词： Honey Myeloperoxidase Chlorination Oxidation Nitration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microscopic Characteristics, Chromatographic Profiles and Inhibition of Peroxidase Activity of the Leaves of <i>Manihot esculenta</i>and <i>Manihot glaziovii</i>, Consumed as Traditional Vegetables

引用

Journal of Biosciences and Medicines 2021年第9期9卷 59-73页

作者： Paulin Mutwale Kapepula Patricia Mbombo Mungitshi Dieudonné Tshitenge Tshitenge thierry franck Dieudonné Mumba Ngoyi Pascal Dibungi T. Kalenda Monique Tits Michel Frédérich Nadege Kabamba Ngombe Ange Mouithys-Mickalad Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Centre for Oxygen Research and Development (CORD) University of Liège Liège Belgium Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of the Congo Laboratory of Pharmacognosy Center for Interdisciplinary Research on Medicines (CIRM) University of Liège Liège Belgium

Methanolic extracts from the leaves of Manihot esculenta (Two cultivars) and Manihot glaziovii, consumed as traditional vegetables in DR. Congo was chemically characterized by Thin layer Chromatography and High Performance Liquid Chromatography. In vitro biochemical activities of extracts against Radical Oxidative Species (ROS) production were assessed in cellular models, on enzymes, Myeloperoxidase (MPO) and Horseradish Peroxidase (HRP) involved in inflammation. The microscopic analysis of the powder of leaves showed that each species displays specific and discriminating botanical microscopic features. Varieties of M. esculenta had a chemical fingerprint different from M. glaziovii. The majority of compounds were polyphenols, represented mainly by rutin, kaempferol-3-O-rutinoside, amentoflavone, phenolic acids such as gallic acid. All extracts exhibited high cellular antioxidant activity in the range of 0.1 to 10 μg·mL-1 using lucigenin with neutrophils, but a moderate cellular antioxidant activity ranging between 10 and 100 μg·mL-1 with DCFDA on HL60 monocytes. Extracts from Manihot leaves showed a pronounced inhibitory effect on the production of extracellular ROS, on HRP and myeloperoxidase activity. Cellular antioxidant activities, the inhibitory effect on HRP of extracts from M. glaziovii, M. esculenta cultivar Mwambu were significantly higher, but their inhibitory effect on the activity of MPO was lower than those of M. esculenta cultivar TEM 419. The biological activities of Manihot esculenta and Manihot glaziovii were well correlated to their phytochemicals that could justify their traditional use as vegetables, potential functional foods or nutraceutical resources and medicines.

关键词： Horseradish Peroxidase Kahemba Konzo Manihot esculenta Manihot glaziovii Myeloperoxidase Traditional Vegetable

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Global Cardiovascular Risk of the HIV Positive Patients Receiving Antiretroviral Therapy in Brazzaville

引用

World Journal of Cardiovascular Diseases 2019年第8期9卷 553-561页

作者： Stéphane Méo Ikama franck Ekoba-Otende Jospin Makani Amélia Bokilo Bienvenu Ossibi-Ibara Louis Igor Ondze-Kafata Bertrand Fikhaem Ellenga-Mbolla thierry Raoul Gombet Suzy Gisèle Kimbally-Kaky Department of Cardiology Brazzaville University Hospital Center Brazzaville Congo National Centre of Blood Transfusion Brazzaville Congo Department of Infectious Diseases Brazzaville University Hospital Center Brazzaville Congo

A cross-sectional, descriptive and analytical study was conducted from January to August 2015 at the Brazzaville Ambulatory Treatment Center and at?the National Blood Transfusion Center. The objective was to contribute to improving?the care of people living with HIV under antiretroviral therapy by assessing their global cardiovascular risk (CVR). The variables studied focused on the epidemiological, clinical and biological aspects. The global CVR was assessed?by the Framingham and WHO/ISH scores. There were 135 HIV-positive subjects,?including 64 treated patients and 71 untreated HIV+ subjects. The subjects were divided into 83 men (61.5%) and 52 women (38.5%), with an average age of 42.6 ± 2.9 old years. The subjects were single people (62.2%), of a secondary educational level (63.7%), and civil servants (32.6%). The main risk factors found were dyslipidaemia (60%), obesity (36%), smoking (12.6%), hypertension (5.9%), diabetes (0.7%). The metabolic syndrome was found in seven cases (11.3%). The global CVR according to the score of Framingham, initially moderated at 17.2%, and mean at 1.5% within treated patients, was mean at 9.4% and high at 1.6% of the subjects respectively at the sixth month of treatment (p CVR under antiretroviral therapy. Preventive actions are highly recommended.

关键词： HIV Infection Antiretroviral Therapy Metabolic Disorders Global Cardiovascular Risk Congo

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

微型图案和等离子辅助渗氮产生的应变组合的不锈钢图案

引用

热处理技术与装备 2011年第3期32卷 11-11页

作者： Gregory Marcos Stephane Guilet franck Cleymand Tony Thiriet thierry Czerwiec

在奥氏体不锈钢上,低温等离子辅助渗氮产生所谓的"扩展奥氏体"。由基体的原始表面沿垂直表面方向产生的这种渗氮层的扩展,此处通常的含义是通过几种掩模选择氮扩散产生的有图案的表面。用的栅网能得到一种精确的正方形点。在这次交流中... 详细信息

在奥氏体不锈钢上,低温等离子辅助渗氮产生所谓的"扩展奥氏体"。由基体的原始表面沿垂直表面方向产生的这种渗氮层的扩展,此处通常的含义是通过几种掩模选择氮扩散产生的有图案的表面。用的栅网能得到一种精确的正方形点。在这次交流中介绍了在奥氏体不锈钢基体上预先用一种有图案的氧化硅层覆盖进行渗氮的结果。也说明了在几种不同形状(园的和正方的点)混合的氧化硅掩膜和扩展奥氏体之间的相互作用。氮的扩散,和实验条件的一致及掩膜的特点非常重要。根据点的尺寸,会导致掩膜简单的均匀变形,或者在掩膜边缘有大畸变的明显变形。这种现象被视为一种环形壳状畸变。光截面似乎证明这仅仅是由于氮扩散正好在掩膜边缘的下面引起奥氏体扩展垂直力的结果。

关键词：奥氏体不锈钢离子辅助渗氮层图案不锈钢基体应变微型表面方向

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：