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Effect of biochar on the metabolome of soybean seedlings

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Oil Crop Science 2024年第1期9卷 60-68页

作者： Nathalia E.silva Mariana G.Aguilar Osania E.ferreira Gleicia M.Paulino Jaqueline C.L.Carvalho Lúcia P.S.Pimenta alan R.T.Machado Programa de Pos-graduaçao em Ciencias Ambientais Universidade do Estado de Minas GeraisFrutalMinas Gerais38202-436Brazil Departamento de Química Universidade Federal de Minas GeraisMinas GeraisBelo Horizonte31270-901Brazil Departamento de Engenharia Aplicada e Tecnologias Ambientais Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil Departamento de Ciencias Exatas Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil

The use of biochar can have several effects on plant germination,depending on raw material,preparation method and application ***,the molecular mechanisms that lead to those results have yet to be *** aim of this research was to improve the understanding of these mechanisms by characterizing the metabolic effects of sugarcane bagasse biochar on soybean *** types of biochars were prepared by pyrolysis at 300℃(SCB300),400℃(SCB400)and 600℃(SCB600).Then,each one was mixed into sand at 1%,3%,5%(w/w)dose,*** experiment was performed in 8 days of incubation,when the number of germinated seeds and the average radicle length were *** evaluate the metabolome,the dry biomass(DB)was subjected to extraction with a mixture of methanol-d4 and D2O(1:1 v/v).The extracts were submitted to metabolomics analysis by Proton Nuclear Magnetic *** Relative Germination,Relative Average Radicle Growth and Germination Index increased in all treatments compared to *** the other hand,the DB increased in all treatments,except for SCB300,at doses of 1%and 3%w/*** metabolites(alanine,asparagine,acetic acid,citric acid,glycerol,fatty acids and sucrose)were identified and quantified in DB extracts as the most influential finding for the separation of *** together,these results strongly suggested that biochars accelerated the catabolism of triacylglycerols to sucrose and induced a slight osmotic stress.

关键词： Germination Metabolomics 1H NMR Pyrolysis Sugarcane bagasse

Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina Pinto Adriana Barboza Alves Osania Emerenciano ferreira Gustavo Henrique Gravatim Costa Magno Andréde Oliveira Augusto Cesar da silva Bezerra alan Rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

Cannabinoid pharmacology and its therapeutic uses in Alzheimer’s disease

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Neural Regeneration Research 2021年第5期16卷 990-991页

作者： Kadja Luana Chagas Monteiro Marcone Gomes dos Santos Alcântara thiago Mendonça de Aquino Edeildo ferreira da silva-Júnior Laboratory of Medicinal Chemistry Institute of Pharmaceutical SciencesFederal University of AlagoasMaceióBrazil Chemistry and Biotechnology Institute Federal University of AlagoasMaceióBrazil

Alzheimer’s disease(AD)is a chronic neurodegenerative disease,which is difficult to diagnose in its early *** is associated with aging and consists of a decline in cognitive functions,memory,and other mental *** pathophysiological markers are associated with AD progression and worsening,such as the deposition and intracellular accumulation of senile plaques containing amyloid-β(Aβ)peptides,and tau protein hyperphosphorylation,leading to the formation of neurofibrillary tangles.

关键词： Alzheimer Aβ tau

Drainage and Preliminary Risk of Flooding in an Urban Zone of Eastern Amazon

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Journal of Geoscience and Environment Protection 2023年第5期11卷 1-16页

作者： Taís silva Sousa Clezio Júnior Teixeira Viegas Helenilza ferreira Albuquerque Cunha alan Cavalcanti da Cunha Graduate Program in Environmental Sciences Federal University of Amapá Macapá Brazil Graduate Program in Tropical Biodiversity Federal University of Amapá Macapá Brazil Graduate Program in Biotechnology Federal University of Amapá Macapá Brazil

Drainage system is a poorly investigated basic sanitation and urban planning issue in the Amazon, which is often related to flooding processes and public health. The aim of the present research is to correlate Preliminary Risk of Flooding (PRF) to independent variables, based on the following methodology: 1) identifying and classifying risk areas by using the Geographic Information System (GIS) and 2) statistically correlating risk to sanitary and environmental variables. Results have shown that preliminary risk is correlated to, at least, seven sanitary and environmental variables, depending on flood influence area;and there are significant correlations observed in the rainy season interval [probability or significance (p) 0.05]. In conclusion, PRF is higher in the rainy season, but it is spatially influenced by the elevation of terrain, number of flooding points, drainage typology and Environmental Salubrity Index (ESI) of neighborhoods, which directly affect the water quality in nearby groundwater wells (Total Coliforms, nitrate (NO3) and ammonia (NH3)). However, this influence can eventually significantly change in the dry season.

关键词： Drainage System Environmental Salubrity Index Groundwater GIS

Hepatobiliary phases in magnetic resonance imaging using liverspecific contrast for focal lesions in clinical practice

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World Journal of Hepatology 2022年第7期14卷 1459-1469页

作者： daniel Alvarenga Fernandes Eduardo Andreazza dal Lago Felipe Aguera Oliver Bruna Melo Coelho Loureiro daniel Lahan Martins thiago José Penachim Ricardo Hoelz de Oliveira Barros José de ArimatéiaBatista Araújo Filho Larissa Bastos Eloy da Costa Áurea Maria Oliveira da silva Elaine Cristina de Ataíde Ilka de Fátima Santana ferreira Boin Nelson Marcio Gomes Caserta Department of Radiology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazi Department of Radiology Medical SchoolSão Paulo State University-UNESPBotucatu 18618-970São PauloBrazil Instituto de Radiologia Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo-InRad/HC-FMUSPSão Paulo 05403-010SPBrazil Department of Radiology Sírio-Libanês HospitalSão Paulo 05652-900SPBrazil Department of Pathology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil Liver Transplant Unit Department of SurgerySchool of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil

BACKGROUND Challenging lesions,difficult to diagnose through non-invasive methods,constitute an important emotional burden for each patient regarding a still uncertain diagnosis(malignant x benign).In addition,from a therapeutic and prognostic point of view,delay in a definitive diagnosis can lead to worse *** of the main innovative trends currently is the use of molecular and functional methods to *** liver-specific contrast agents havebeen developed and studied in recent years to improve the performance of liver magnetic resonance imaging(MRI).More recently,one of the contrast agents introduced in clinical practice is gadoxetic acid(gadoxetate disodium).AIM To demonstrate the value of the hepatobiliary phases using gadoxetic acid in MRI for the characterization of focal liver lesions(FLL)in clinical *** Overall,302 Lesions were studied in 136 patients who underwent MRI exams using gadoxetic acid for the assessment of *** radiologists independently reviewed the MRI exams using four stages,and categorized them on a 6-point scale,from 0(lesion not detected)to 5(definitely malignant).The stages were:stage 1-images without contrast,stage 2-addition of dynamic phases after contrast(analogous to usual extracellular contrasts),stage 3-addition of hepatobiliary phase after 10 min(HBP 10’),stage 4-hepatobiliary phase after 20 min(HBP 20’)in addition to stage *** The interobserver agreement was high(weighted Kappa coefficient:0.81-1)at all stages in the characterization of benign and malignant *** diagnostic weighted accuracy(Az)was 0.80 in stage 1 and was increased to 0.90 in stage *** of the hepatobiliary phase increased Az to 0.98 in stage 3,which was also 0.98 in stage *** The hepatobiliary sequences improve diagnostic *** growing potential in the era of precision medicine,the improvement and dissemination of the method among medical specialties can bring benefits in the management of patients w

关键词： Liver Liver neoplasms Liver transplantation Medical oncology Diagnostic imaging Magnetic resonance imaging

Sublethal Doses of Insecticides Affect the Fecundity and Fertility of the Chrysodeixis includens?

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American Journal of Plant Sciences 2018年第3期9卷 483-494页

作者： Raimundo Henrique ferreira Rodrigues Luciana Barboza da silva thiago ferreira Rodrigues Gleidyane Novais Lopes Eliane Carneiro Bruno Ettore Pavan Post-Graduation Program in Plant Science Agronomy Universidade Federal do Piauí Bom Jesus Brazil Faculty of Engineering UNESP Ilha Solteira Universidade Estadual Paulista Ilha Solteira Brazil

The objective was to determine lethal concentrations and to evaluate the effects of sublethal doses of insecticides on Chrysodeixis includens under laboratory conditions. The standard population of C. includens was kept on artificial diet under controlled conditions (25°C ± 5°C, 60% ± 10 RU, 12:12 h). The active principles used were B. thuringiensis, Indoxacarb and Teflubenzuron. The CL25 and LC50 obtained from dose response curve (estimated from eight concentrations of each active principle) were diluted in water and applied to the back of third instar caterpillars and compared to the control (water). The insecticides used showed lethal and sublethal effects in C. includens. Bacillus thuringiensis was 6.21 and 2.79 times more toxic to soybean looper when compared to the products Indoxacarb and Teflubenzuron, respectively. Insecticides applied to the caterpillars reduced survival and affected the biomass gain with increased larvae and pupae longevity, with a significant reduction in fertility (40%) and fecundity (94.97%) in adults. In the present study it was observed that both survival and larval biomass gain have a direct effect on fecundity and fertility, respectively. Therefore the active ingredients Indoxacarb, Teflubenzuron and B. thuringiensis can be used in rotation as a tool in the C. includens.

关键词： Bacillus thuringiensis Chemical Control Indoxacarb Soybean Looper Teflubenzuron

Comparative Study of Helicoverpa armigera(Hubner) (Lepidoptera: Noctuidae) Preference for Bt and Non-Bt Soybean Cultivars

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American Journal of Plant Sciences 2017年第10期8卷 2483-2500页

作者： Vilmar Bueno Dos Santos Luciana Barboza silva Eliane Carneiro Alexandre Faria silva Gleidyane Novais Lopes Bruno Ettore Pavan thiago ferreira Rodrigues Postgraduate Program in Plant Science Agronomy Universidade Federal do Piauí (UFPI) Bom Jesus Piauí FacultyofEngineering UNESP Ilha Solteira Universidade Estadual Paulista Ilha Solteira Brasil

Studies on the behavior of Helicoverpa armigera in relation to soybean cultivars expressing Bt proteins are fundamentally important for IPM. We determine the no-choice and feeding choice of H. armigera, the no-choice and oviposition choice in different Bt and non-Bt soybeans cultivars. In the first step it was carried out the experiment to determine the no-choice and food choice in test for 12 Bt and non-Bt soybean cultivars. Simultaneously the consumption was determined. In a second step it was evaluated the no-choice and oviposition choice in different soybean cultivars. Helicoverpa armigera caterpillars showed neither attractiveness and preference for food nor attractiveness and oviposition preference for Bt and non-Bt soybean leaves when simultaneously contrasting the tested cultivars. This study showed that Bt toxin did not influence the oviposition preference, and H. armigera adults did not differentiate Bt and non-Bt soybean cultivars for oviposition indicating no behavioral preference. Third instar caterpillars showed no discrimination between Bt and non-Bt soybean leaves. Research in this area must focus on the possibility of widespread planting of genetically modified soybeans containing the Bt protein, which is associated with selection pressure and the behavior of pest species in relation to their hosts, as well as on the adequacy of management tactics that is able to prevent the loss of technology efficiency.

关键词： Cry1Ac Protein Genetically Modified Plants Food Preference Oviposition

Evaluation of Insecticides Targeting Control of Helicoverpa armigera(Hubner)(Lepidoptera:Noctuidae)

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American Journal of Plant Sciences 2014年第18期5卷 2823-2828页

作者： Eliane Carneiro Luciana Barboza silva Kellen Maggioni Vilmar Buenos dos Santos thiago ferreira Rodrigues Soislan Souza Reis Bruno Ettore Pavan Programa de Pos Graduacao em Agronomia Fitotecnia Universidade Federal do PiauíCampus Prof.CinobelinaElvasBom JesusBrazil Graduacao em Agronomia Universidade Federal do Piauí Campus Prof.CinobelinaElvasBom JesusBrazil Faculdade de Engenharia UNESP Ilha Solteira Universidade Estadual PaulistaIlha SolteiraBrazil

Insecticides from different chemical groups were tested by laboratory bioassay to verify the percentage mortality of Helicoverpa armigera (Hubner 1808) (Lepidoptera: Noctuidae). The experiment was conducted in the Crop Science laboratory—Prof. CinobelinaElvas Campus—UFPI, Bom Jesus, PI, from January to June, 2013. The populations utilized came from the University’s own insect breeding laboratories. Third instar larvae of H. armigera were used to conduct the bioassay. The experimental design was fully randomized, with 13 treatments and four replications. Five larvae were used per replication, with 12 insecticides from 9 different chemical groups and a control. Each treatment consisted of three doses. The methods of application used were topical contact and ingestion in artificial diet. According to the results the percentage mortality of H. armigera larvae varied among the treatments. The results demonstrated that chlorpyrifos and spinosad were effective against third instar H. armigera larvae both on contact and by ingestion. Flubendiamide, acephate, methomyl, Bacillus thuringiensis, dimethoate, chlorantraniliprole and fipronil had good responses to control of H. armigera.

关键词： Bollworm Bioassay Chemical Control Insecticides Soybeans

Early expression of the tbx22 gene in zebrafish influences positioning of pharyngeal arch cartilages

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American Journal of Molecular Biology 2012年第4期2卷 318-331页

作者： Tammy Lynne silva Angela Allard Cox V. P. Boominathan Peter alan Jezewski Tracie Lee ferreira Department of Biology University of Massachusetts Dartmouth Dartmouth USA Department of Bioengineering University of Massachusetts Dartmouth Dartmouth USA Department of Periodontology Institute of Oral Health Research University of Alabama at Birmingham Birmingham USA

Mutations in human TBX22 cause X-linked cleft palate with anklyoglossia syndrome. The two zebrafish tbx22 splice isoforms, tbx22-1 and tbx22-2, encode proteins of 444 and 400 amino acids, respectively. Zebrafish tbx22 mRNA expression mirrors mammalian TBX22 expression and is consistent with early patterning of the vertebrate face. In zebrafish, tbx22 mRNA is strongly expressed during early pharyngeal arch development in the ventral mesenchyme, and a later expression domain is found in ectomesenchymal cells underlying the stomodeum, a bilaminar epithelial structure demarcating the early forming mouth. Therefore, tbx22 is hypothesized to be involved in craniofacial development. The objective of this work is to characterize the role of tbx22 during craniofacial development in zebrafish. Tbx22 knockdown revealed that defects in tbx22 signaling cause mild clefting, joint defects and dorsoventral patterning defects in cartilages. Quantitative PCR and in situ analysis revealed that knockdown of tbx22 also causes a dramatic decrease in expression of osr1 and gdf5. Craniofacial patterning is dependent on proper signals from endoderm, mesoderm and ectoderm. The early influence of tbx22 on signals within the ventral mesenchyme impacts the domains of several key pharynx-geal arch signals, thereby helping to regulate proper patterning of the developing jaw.

关键词： Zebrafish Tbx Signaling Pharyngeal Pouch