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Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 25-35页

作者： Quezada-Rivera JJ RE Soria-Guerra FS Pérez-Juárez L Martínez-González SE Valdés-Rodríguez NL Vasco-Méndez JF Morales-domínguez Departamento de Química Universidad Autónoma de AguascalientesUniversidad No.940Ciudad UniversitariaC.P.20131AguascalientesAgs.México Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíZona Universitaria PonienteC.P.78290San Luis PotosíS.L.P.México Departamento de Biotecnología y Bioquímica Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCinvestav-Unidad Irapuato.Km.9.6 Libramiento Norte Carr.Irapuato-León 36824 IrapuatoGto.México

The use of antimicrobial peptides(AMPs)synthesized by bacteria(bacteriocins)is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass *** bacteriocin E-760 isolated from the genus Enterococcus *** been shown to possess inhibitory activity against Gram-negative and Gram-positive *** this study,the expression of a chimeric protein coding for E-760 in the nucleus of *** was evaluated,as well as,its antibacterial *** synthetic gene E-760S was inserted into the genome of *** using Agrobacterium tumefaciens.A transgenic line was identified in TAP medium with hygromycin and also by *** increment in the culture medium temperature of the transgenic strain at 35°C for 10 minutes,increased the production level of the recombinant protein from 0.14(Noninduced culture,NIC)to 0.36%(Induced culture,IC)of total soluble proteins(TSP);this was quantified by an ELISA *** E-760 possesses activity against Staphylococcus aureus in 0.34 U log,Streptococcus agalactiae in 0.48 U log,Enterococcus faecium in 0.36 U log,Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae,the activity was 0.07 U *** results demonstrate that the nucleus transformation of *** can function as a stable expression platform for the production of the synthetic gene E-760 and it can potentially be used as an antibacterial agent.

关键词： Heterologous expression Antibacterial activity Bacteriocin E-760 Chlamydomonas reinhardtii Log inactivation Nuclear transformation

Potential seed germination-enhancing plant growth-promoting rhizobacteria for restoration of Pinus chiapensis ecosystems

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Journal of Forestry Research 2021年第5期32卷 2143-2153页

作者： Cristina domínguez-Castillo Julia María Alatorre-Cruz Dolores Castañeda-Antonio Jose Antonio Munive Xianwu Guo Jesús Francisco López-Olguín Luis Ernesto Fuentes-Ramírez Ricardo Carreño-López Centro de Investigaciones en Ciencias Microbiológicas Instituto de CienciasBenemérita Universidad Autónoma de PueblaPueblaMéxico Universidad Autónoma de Querétaro QuerétaroMéxico Centro de Biotecnología Genómica Instituto Politécnico NacionalReynosaTamaulipasMéxico

Rhizosphere soil samples of three Pinus chiapensis sites were analyzed for their physicochemical properties,soil bacteria isolated and screened in vitro for growthpromoting *** isolates that showed promise were identified to five genera Dyella,Luteimonas,Euterobacter,Paraburkholderia and Bacillus based on the sequences of16 S rRNA *** the strains were isolated from nondisturbed *** bacteria significantly decreased germination time and increased sprout *** acetic acid and gibberellin production and phosphate solubilisation were *** indicate that these biochemicals could be essential for *** distribution and suggest the possibility that PGPR inoculation on *** seeds prior to planting could improve germination and possibly seedling development.

关键词： Pinus chiapensis Plant growth-promoting rhizobacteria Dyella Luteimonas Enterobacter

Use of Cucurbit [6] Uril as a Modifier in the Electrochemical Determination of Antitumor Platinum (II) Complex: Trans-[PtCl2(Dimethylamine) (Isopropylamine)]. Application to Biological Samples

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American Journal of Analytical Chemistry 2013年第6期4卷 314-322页

作者： Carmen S. H. domínguez Pedro Hernández Departamento de Química Analítica y Análisis Instrumental Facultad de Ciencias Universidad Autónoma de Madrid Cantoblanco Madrid Spain

A square wave voltammetry (DPV) method for trans-Pt[Cl2(Dimethylamine)(isopropylamine)] determination is developed. To this end, all the chemical and instrumental variables affecting the determination of trans-Pt[Cl2(Dimethylamine) (isopropylamine)] are optimized. From studies of the mechanisms governing the electrochemical response of trans-Pt[Cl2(Dimethylamine)(isopropylamine)], it was concluded that it was an electrochemically reversible system with an adsorptive oxidation phenomenon. Under optimal conditions, the variation of analytical signal (Ip) with trans-Pt[Cl2(Dimethylamine)(isopropylamine)] concentration was linear in the 0.05 μg·mL-1 to 10 μg·mL-1 range, with a LOD 91 μg·mL-1 of and a LOQ of 303 μg·mL-1, a RSD 1.10% and Er 0.72%. The optimized method was applied to the determination of trans-Pt[Cl2(Dimethylamine)(isopropylamine)] in biological fluids, in human urine and synthetic urine.

关键词： Trans-[PtCl2(Dimethylamine)(Isopropylamine)] Differential Pulse Voltammetry (DPV) Square Wave Voltammetry (SWV) Biological Fluids Human Urine

Methodology of the data processing with ICT solutions for the evaluation of the energy and water savings

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Methodology of the data processing with ICT solutions for th...

As Regards the Speed in a Medium of the Electromagnetic Radiation Field

The 2nd International Conference on Green Building Technologies and Materials(GBTM2012)

作者： Samuel domínguez Jessica Fernández-Agüera Rocío Escandn Eugenio domínguez Institute of Architecture and Building Science University of Seville2 Reina Mercedes Avenue41012 SevilleSpain

EnergyTIC(Technology,Information and Communication services for engaging social housing residents in energy and water efficiency) has put information and communication technology(ICT) solutions in place to provide end-users with methods to monitor and adapt their energy and water *** main objective of this paper is to establish a methodology to analyze,process,and organize the user information and the energy and water consumption information from the Spanish pilot *** will work with three different layers of information: (1) data provided to the user,(2) data managed for the analysis,and(3) data entered into the software "eeMeassure" to quantify the savings according to the unified criteria of the European Commission..

关键词： ICT solutions Energy Efficiency Energy and water savings Energy user’s feedback

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Journal of Modern Physics 2016年第11期7卷 1320-1330页

作者： Robert M. Yamaleev A. R. Rodríguez-domínguez Joint Institute for Nuclear Research Dubna Russia Instituto de Física Universidad Autónoma de San Luis Potosí San Luis Potosí México

The velocity of the electromagnetic radiation in a perfect dielectric, containing no charges and no conduction currents, is explored and determined on making use of the Lorentz transformations. Besides the idealised blackbody radiation, whose vacuum propagation velocity is the universal constant c, being this value independent of the observer, there is another behaviour of electromagnetic radiation, we call inertial radiation, which is characterized by an electromagnetic inertial density , and therefore, it happens to be described by a time-like Poynting four-vector field which propagates with velocity . is found to be a relativistic invariant expressible in terms of the relativistic invariants of the electromagnetic field. It is shown that there is a rest frame, where the Poynting vector is equal to zero. Both phase and group velocities of the electromagnetic radiation are evaluated. The wave and eikonal equations for the dynamics of the radiation field are formulated.

关键词： Inertial Radiation Field Mass Field Density Rest State Poynting Vector Wave and Eikonal Equations of the Radiation Field Dynamics

Effect of Electric Field on the Kinetics of Growth of Lettuce (Lactuca sativa) in a Hydroponic System

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Journal of Agricultural Chemistry and Environment 2016年第3期5卷 113-120页

作者： Orlando Fuentes-Castañeda Martha Lilia domínguez-Patiño Jorge domínguez-Patiño Rosa María Melgoza-Alemán Oscar Gabriel Villegas-Torres CIICAp Universidad Autónoma del Estado de Morelos Cuernavaca México Facultad de Ciencias Químicas e Ingeniería Universidad Autónoma del Estado de Morelos Cuernavaca México Facultad de Ciencias Agropecuarias Universidad Autónoma del Estado de Morelos Cuernavaca México

The implementation of alternative harvest systems which are able to accelerate the kinetics of growth of plants, is extremely important to decrease the time of crop. In this sense, Electro-Hydroponic culture, i.e., the application of an electric field in the nutrient solution of the hydroponic culture emerges as an alternative and interesting harvest system. The objective of the present study was to investigate the effect of the electric field applying different intensities of direct current (DC) at the galvanostatic regime (50, 37.5, 25 and 12.5 mA) on the growth of lettuce (Lactuca sativa) plants in hydroponic culture. Fifty lettuce plants were used in a nutrient solution, from which 4 groups composed of ten lettuces each one, were subjected at 12.5, 25, 37.5 and 50 mA, respectively. A fifth group of ten lettuces was used as a reference test, i.e., without application of DC. The experiments were carried out during 16 days. The results reveal that the kinetics of growth of the lettuce is dependent on the applied current;actually it is observed an increase in the foliate area of the plants cultivated in the presence of DC than that obtained in the reference test. Furthermore, the plants subjected at 12.5 mA, revealed an increase in the foliate area of 65.3% and 73.2% in comparison with the reference test.

关键词： Hydroponic Direct Current Lettuce Galvanostatic Regime

Autolytic and Proteolytic Properties of Strains ofLactococcus lactisIsolated from Different Vegetables, Raw-Milk Cheeses and Commercial Starter Cultures

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Food and Nutrition Sciences 2013年第11期4卷 21-26页

作者： Carolina Nájera-domínguez Nestor Gutiérrez-Méndez Chemistry Faculty University Autonomous of Chihuahua Chihuahua Mexico.

The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. The autolytic and proteolytic activity of L. lactis has been linked with the development of flavor and texture in the cheese. On the other hand, there is a growing interest in new strains isolated from raw-milk cheeses and vegetables. These wild-strains have showed different features of industrial importance in comparison with those observed in commercial cultures. However, it still not clear if the autolytic and proteolytic properties of these wild-strains differ from the industrial strains. The objective of this work was to assess the autolytic and proteolytic activities of 21 strains of L. lactis isolated from diverse sources. The rates of autolysis and proteolysis observed in vitro were highly strain-dependent. The pH and the NaCl concentration in the media affected significantly the autolysis of L. lactis. The strains isolated from vegetable showed in general low and medium autolytic activity, whereas the strains isolated from raw-milk cheeses had medium to high autolytic activity. The strain with highest proteolytic activity was a strain isolated from corn leaves. Although still not clear how this strain acquired this pronounced characteristic.

关键词： Com Autolysis Proteolysis L. lactis Wild-Strains

Management of Crohn’s disease in smokers:Is an alternative approach necessary?

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World Journal of Gastroenterology 2011年第31期17卷 3567-3574页

作者： Pilar Nos Eugeni domènech Gastroenterology Unit La Fe University Hospital Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CI-BEREHD) MadridSpain Digestive Disease Department Germans Trias i Pujol Hospital

Inflammatory bowel disease(IBD) is a chronic condition with a pathogenic background that involves both genetic and environmental *** important progress has been made regarding the former in the last decade,scarce knowledge is available for the *** this sense,smoking remains the most important environmental factor in *** smoking increases the risk of developing Crohn's disease(CD).Moreover,CD patients who start or continue smoking after disease diagnosis are at risk for poorer outcomes such as higher therapeutic requirements and disease-related complications,as compared to those patients who quit smoking or who never ***,the harmful effect of active smoking is not uniform in all patients or in all clinical *** designed to facilitate smoking cessation may impact the course of the *** this article,the available evidence of the deleterious effects of smoking on CD is reviewed in detail,and alternative therapeutic approaches to CD in smokers are proposed.

关键词： Tobacco Crohn's disease Therapy Smoking cessation Recurrence Complications

Whole lesion histogram analysis of apparent diffusion coefficient predicts therapy response in locally advanced rectal cancer

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World Journal of Gastroenterology 2022年第23期28卷 2609-2624页

作者： Mayra Evelia Jiménez de los Santos Juan Armando Reyes-Pérez Victor domínguez Osorio Yolanda Villaseñor-Navarro Liliana Moreno-Astudillo Itzel Vela-Sarmiento Isabel Sollozo-Dupont Department of Radiology National Cancer InstituteMexico 14080Mexico Department of Gastrointestinal Surgery National Cancer InstituteMexico 14080Mexico

BACKGROUND Whole-tumor apparent diffusion coefficient(ADC)histogram analysis is relevant to predicting the neoadjuvant chemoradiation therapy(nCRT)response in patients with locally advanced rectal cancer(LARC).AIM To evaluate the performance of ADC histogram-derived parameters for predicting the outcomes of patients with *** This is a single-center,retrospective study,which included 48 patients with *** patients underwent a pre-treatment magnetic resonance imaging(MRI)scan for primary tumor staging and a second restaging MRI for response *** sample was distributed as follows:18 responder patients(R)and 30 non-responders(non-R).Eight parameters derived from the whole-lesion histogram analysis(ADCmean,skewness,kurtosis,and ADC10^(th),25^(th),50^(th),75^(th),90^(th) percentiles),as well as the ADCmean from the hot spot region of interest(ROI),were calculated for each patient before and after *** all data were compared between R and non-R using the Mann-Whitney U *** measures of diagnostic accuracy were applied:the receiver operating characteristic curve and the diagnostic odds ratio(DOR).We also reported intra-and interobserver variability by calculating the intraclass correlation coefficient(ICC).RESULTS Post-nCRT kurtosis,as well as post-nCRT skewness,were significantly lower in R than in non-R(both P<0.001,respectively).We also found that,after treatment,R had a larger loss of both kurtosis and skewness than non-R(Δ%kurtosis and Δ skewness,P<0.001).Other parameters that demonstrated changes between groups were post-nCRT ADC10^(th),Δ%ADC10^(th),Δ%ADCmean,and ROIΔ%***,the best diagnostic performance was achieved byΔ%kurtosis at a threshold of 11.85%(Area under the receiver operating characteristic curve[AUC]=0.991,DOR=376),followed by post-nCRT kurtosis=0.78×10^(-3)mm^(2)/s(AUC=0.985,DOR=375.3),Δskewness=0.16(AUC=0.885,DOR=192.2)and post-nCRT skewness=1.59×10^(-3)mm^(2)/s(AUC=0.815,DOR=168.6).Finally,intraclass corr

关键词： Apparent diffusion coefficient Diffusion-weighted imaging Histogram analysis Magnetic resonance imaging Locally advanced rectal cancer