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SARS-CoV-2 Epitopes follow.ng Infection and Vaccination Overlap Know. Neutralizing Antibody Sites

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Research 2022年第2期2022卷 71-84页

作者： Li Yang Te Liang Lane M.Pierson Hongye w.ng Jesse K.fletcher Shu w.ng Duran Bao Lii Zhang Zhen Huang w.nshu Zheng Xiaomei Zhang Heew.n Park Yuw.n Li James E.Robinson Amy K.Fechan Christopher J.Lyon Jing Cao Lisa A.Morici Chenzhong Li Chad J.Roy Xiaobo Yu Tony Hu Center for Cllular and Molecular Diagnostics Tulane University Schoo of Medicine1430 Tulane AveNew OrleansLA 70112USA Department of Biochemisty and Molecular Biology Tulane University School of Medicine1430 Tulane AveNew OrleansLA 70112USA Beijing Key Laboratory for Forest Pest Control Bejjing Foresiry UniersilyBeijing 10083.China State Key Laboratory of Proteommics Beijing Protcome Resarch CenterNattional Center for Protein Sciences-Beiing(PHOENIX Center)Beijing Institute of LijfeomicsBeijing 102206China Hayw.rd Genetis Cener Department of PediatricsTulane Universily School of Medicine1430 Tulane AveNew OrleansLA 70112USA Department of Pediatrics Tulane Uhriversity School of Medicine1430 Ttulane AveNew OrleansLA 70112USA Infectious Disease Depariment Ochsner Clinic FoundationNew OrleansLA 70121USA Universty of Texas Souhw.stern Medical Cener 5323 Harry Himes BlvdDallasTX 75390USA Department of Microbiology Imruolog Tulane Untiversity School of Medicine 1430 Tulane AveNew OrleansLA 70112USA Division of Microbiology Tulane National Primate Research Center18703 Three Rivers RoadCovingtonLA 70433USA

Identification of epitopes targeted follow.ng virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be ***,w. employed peptide microarrays to map linear peptide epitopes(LPEs)recognized follow.ng SARS-CoV-2 infetion and *** detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned w.th reported neutralizing antibody binding *** LPE overlap occurred atfter infection and vaccination,w.th LPE clusters specifc to each stimulus,w.ere strong and conserved LPEs mapping to sites know. or likely to inhibit spike protein ***-specifc LPEs tended to map to sites know. or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine's S *** LPEs to regions of know. functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.

关键词： vaccine Antibody likely

Dual inhibition of ACK1 and KIT in Gastrointestinal Stromal Tumor

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Dual inhibition of ACK1 and KIT in Gastrointestinal Stromal ...

Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe-/-mice

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Jiaming Chen Jiongyan Ding Adrián Mari?o-Enríquez Jonathan A.fletcher w.n-Bin Ou Zhejiang Provincial Key Laboratory of Silkw.rm Bioreactor and Biomedicine College of Life Sciences and Medicine Zhejiang SciTech University Department of Pathology Brigham and Women’s Hospital and Harvard Medical School

Gastrointestinal stromal tumor(GIST) is the most common malignant tumor of gastrointestinal *** and activated Oncogene KIT and PDGFRA mutation and abnormal activation are the main factors for the occurrence and progression of *** have KIT(85%) or PDGFRA(10%) gene mutations. At present, KIT has become an important tumor biomarker for the diagnosis of GIST. Imatinib(IM), a specific KIT/PDGFRA inhibitor, become a firstline drug for the treatment of GIST. Clinical studies have demonstrated that IM has a remarkable benefit for GIST patients. How.ver, most of the patients eventually acquired resistance to IM after treatment w.th drugs for tw. years. The second-line drug sunitinib has a good effect on IM resistant GISTs, but it has badly clinical side effects. Therefore, it is urgent to solve the problem of GIST IM ***1 is a member of the non-receptor tyrosine kinase family, studies have reported that ACK1 has abnormal expression and activation in many kinds of tumors, such as prostate cancer, hepatocellular carcinoma, breast cancer, stomach cancer, pancreatic cancer and so on. Therefore, in the present project, w. focused on studying the expression, activation and biological function of ACK1 and the additive effects of dual targeting ACK1 and KIT on GIST signaling pathw.y, proliferation and metastasis. The results show.d that ACK1 overexpressed in GIST cell lines and tumor samples. In addition, it w.s observed that ACK1 strongly phosphorylated in IM sensitive GIST cell lines(GIST-T1) and IM resistant GIST cell line(GIST 430) by Coimmunoprecipitation(Co-IP). How.ver, there is no interaction betw.en ACK1 and KIT proteins. Inhibition of KIT(IM and Sunitinib) did not reduce ACK1 phosphorylation, and inhibition of ACK1(AIM-100) little affected KIT activation. Treatment w.th AIM-100 inhibited ACK1 activation in a dose-dependent manner, w.ich further verified that there is no interaction betw.en ACK1 and KIT. MTT assay show.d that ACK1 inactivation on

关键词： GIST ACK1 KIT AIM-100 IM resistance

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w.rld Journal of Gastrointestinal Pathophysiology 2017年第2期8卷 67-76页

作者： Mandy Heritage Lesley Jaskow.ki Kim Bridle Catherine Campbell David Briskey Laurence Britton Linda fletcher Luis Vitetta V Nathan Subramaniam Darrell Craw.ord School of Medicine The University of Queensland Gallipoli Medical Research Institute Greenslopes Private Hospital Envoi Specialist Pathologists School of Human Movement and Nutrition Sciences The University of Queensland Department of Gastroenterology and Hepatology Princess Alexandra Hospital The University of Sydney Sydney Medical School Medlab Clinical Ltd The QIMR Berghofer Medical Research Institute Institute of Health and Biomedical Innovation Queensland University of Technology

AIM To investigate the synergistic hepato-protective properties of curcumin and vitamin E in an Hfe^(-/-)high calorie diet model of *** Hfe^(-/-)C57BL/6J mice w.re fed either a high calorie diet or a high calorie diet w.th 1 mg/g curcumin; 1.5 mg/g vitamin E; or combination of 1 mg/g curcumin + 1.5 mg/g vitamin E for 20 w.. Serum and liver tissue w.re collected at the completion of the experiment. Liver histology w.s graded by a pathologist for steatosis, inflammation and fibrosis. RNA and protein w.s extracted from liver tissue to examine gene and protein expression associated w.th fatty acid oxidation, mitochondrial biogenesis and oxidative stress *** Hfe^(-/-)mice fed the high calorie diet developed steatohepatitis and pericentral fibrosis. Combination treatment w.th curcumin and vitamin E resulted in a greater reduction of percent steatosis than either vitamin E or curcumin therapy alone. Serum alanine aminotransferase and non-alcoholic fatty liver disease(NAFLD) activity score w.re decreased follow.ng combination therapy w.th curcumin and vitamin E compared w.th high calorie diet alone. No changes w.re observed in inflammatory or fibrosis markers follow.ng treatment. Epididymal fat pad w.ights w.re significantly reduced follow.ng combination therapy, how.ver total body w.ight and liver w.ight w.re unchanged. Combination therapy increased the m RNA expression of Adipo R2, Ppar-α, Cpt1 a, Nrf-1 and Tfb2 m suggesting enhanced fatty acid oxidation and mitochondrial biogenesis. In addition, combination treatment resulted in increased catalase activity in Hfe^(-/-)mice. CONCLUSION Combination curcumin and vitamin E treatment decreases liver injury in this steatohepatitis model, indicating that combination therapy may be of value in NAFLD.

关键词： Non-alcoholic fatty liver disease Hemochromatosis Iron overload Steatosis High calorie diet

白细胞介素-2的生物化学和临床药理学研究进展

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国际免疫学杂志 1988年第3期 144-146页

作者： fletcher M Goldstein AL 曾美麟

白细胞介素-2（IL-2）的分子生物学人类IL-2为133个氨基酸糖蛋白,分子量为15 420,其基因位于第4对常染色体长臂的q26-28处,重组IL-2（rIL-2）的二级结构为α-螺旋,沉降系数1.86S,等电点（PI）7.7。2-4×10～6μ/mg时有诱导鼠HT2细胞系的... 详细信息

白细胞介素-2（IL-2）的分子生物学人类IL-2为133个氨基酸糖蛋白,分子量为15 420,其基因位于第4对常染色体长臂的q26-28处,重组IL-2（rIL-2）的二级结构为α-螺旋,沉降系数1.86S,等电点（PI）7.7。2-4×10～6μ/mg时有诱导鼠HT2细胞系的DNA合成。原级转译产生的小鼠IL-2有169个氨基酸（包括一个信号肽）,其特征为12个连贯的谷氨酸残基靠近氨基端。编码鼠IL-2的核苷酸位为有3个内含子的7Kilobase基因组DNA片段。小鼠和人的外含子无同源性,但在调节区间有明显同源性。

关键词：白细胞介素剂量用药量 IL 抑制因子细胞系小鼠小家鼠环磷酞胺 LAK

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用于扬声器锥盆的新一代聚丙烯材料

电声技术 2003年第6期27卷 76-76页

作者： Brent fletcher 香港海帆国际有限公司

耐克IHM是耐克有限公司的子公司,它位于美国密苏里州圣路易斯郊外的圣查尔斯的办公室和厂房很庞大,拥有20438m2(220000ft2)。耐克公司的主要产品除了体育运动方面之外,还为美国所有的锥盆制造商提供用于折环和防尘帽的Santoprene热塑性... 详细信息

耐克IHM是耐克有限公司的子公司,它位于美国密苏里州圣路易斯郊外的圣查尔斯的办公室和厂房很庞大,拥有20438m2(220000ft2)。耐克公司的主要产品除了体育运动方面之外,还为美国所有的锥盆制造商提供用于折环和防尘帽的Santoprene热塑性橡胶。耐克公司2002年推出了用于锥盆的聚丙烯片材(以下简称为PP——译注),现在又开发了一种超高性能的PP配方,这使得PP锥盆能够应用于那些曾经嫌PP太软和太重的场合。这产品要求绝非一般的塑料挤出成形技术。NikeIHM在化合、研究、开发、技术和制造方面的专业知识,使它具备了解决一系列扬声器软件性能方面问题的独特能力。PP音盆锥盆是扬声器的最重要部件。设计工程师知道扬声器音质在相当的程度上依赖于锥盆。在扬声器里安装一个特殊的音圈没人会知道,而使用一个没有瑕疵、表面处理很高级的锥盆,每个人都会马上注意到。大约30年前,当英国B&w.司推出Bendextrene低音扬声器时,其热塑性塑料锥盆造成了相当的轰动。它的失真比纸盆的低,但这种聚合材料的厚度不够一致,这是要求精确厚度的扬声器锥盆所不允许的。不久,人们发现PP是热塑性扬声器锥盆的首选树脂片材。PP锥盆既受家庭影...

关键词：扬声器锥盆聚丙烯材料耐克IHM公司 Santoprene热塑性橡胶

Characteristics of the Chinese subjects entered the Hypertension in the Very Elderly Trial

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Chinese Medical Journal 2008年第16期121卷 1509-1512页

作者： LIU Li-sheng w.NG Ji-guang MA Shu-ping w.NG w.n LU Fang-hong ZHANG Liang-qing w.nston Banya Ruth Peters Nigel Beckett Astrid fletcher Christopher J. Bulpitt Beijing Hypertension League Institute Beijing 100039 China Centre for Epidemiological Studies and Clinical Trials Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Cardiology Hebei Provincial Hospital Shijiazhuang Hebei 050051 China National Centre for Cardiovascular Disease Fuwai Hospital Chinese Academy of Medical Sciences Beijing 100037 China Shandong Academy of Medical Sciences Jinan Shandong 050051 China Shanxi Cardiovascular Institute Taiyuan Shanxi 030001 China Care of the Elderly Hammersmith Hospital Imperial College London UK

Background The baseline characteristics of patients in a multinational trial are possibly related to cardiovascular outcome. This study compared the baseline characteristics of patients recruited in China w.th those recruited in other countries. Methods A total of 508 Chinese hypertensive men and 728 w.men over the age of 80 years w.o entered the Hypertension in the Very Elderly Trial （HYVET） w.re compared w.th those in 860 men and 1348 w.men w.o entered the trial in other countries. Results The Chinese subjects w.re slightly younger, had less previous hypertension but more previous strokes than the subjects from other countries. The Chinese subjects smoked more than those from other countries, but drank less alcohol. They had less previous episodes of myocardial infarction and w.re, on average, lighter and shorter. The Chinese had low.r mean concentrations of blood urea, uric acid and creatinine as w.ll as higher concentrations of high density lipoprotein （HDL） cholesterol. The concentration of total cholesterol w.s, on average, low.r in the Chinese subjects as w.s blood glucose. The levels of serum sodium and potassium, blood hematocrit and hemoglobin w.re all, on average, low.r in the Chinese subjects. Conclusions Calorie restriction, compared w.th the rest of the w.rld, may have resulted in low.r stature and w.ight, and recent increases in calorie intake have not changed the metabolic profile of the very elderly hypertensive patients in China. Some of these biochemical differences may reflect different dietary lifestyle in the Chinese.

关键词： elderly epidemiology hypertension China

国际输血协会委员会对红细胞表面抗原的命名:温哥华报告

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国外医学（输血及血液学分册） 2004年第4期27卷 367-368页

作者： Daniels GL Cartron JP fletcher A 吴俊杰浙江省血液中心

2002年在加拿大温哥华举行的国际输血协会委员会会议上对红细胞命名作了修改.原命名系统于1995年修订,并分别在1996、1998、2000年有调整.现在最新的分类信息可以在血型命名学网站(http://***/page25htm)查到.Rh、Lutheran、Kell、Crome... 详细信息

2002年在加拿大温哥华举行的国际输血协会委员会会议上对红细胞命名作了修改.原命名系统于1995年修订,并分别在1996、1998、2000年有调整.现在最新的分类信息可以在血型命名学网站(http://***/page25htm)查到.Rh、Lutheran、Kell、Cromer和Knops系统都增加了新的抗原.另外创建了Ⅰ、Globoside、GIL等3个新的血型系统(27～29系统)(见表1).对RH1(D)和RH1变异体抗原的表位新的命名法达成了一致,结果将单独报道.

关键词：国际输血协会委员会红细胞表面抗原命名温哥华报告

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建设项目采购方式与中国建筑市场的改革

建筑 2001年第8期 17-19页

作者：阎长俊,王仁波,赵金力,Matthew.fletcher 沈阳建筑工程学院

人类社会的商品交换从以物易物 ,经过以货币为媒介进行交易 ,到电子购物 ,商品交易范围日益扩大。今天 ,通过网络可以在全球范围实现在线即时交易。建筑产品作为大宗交易的特殊商品 ,它的购买方法不同于一般商品 ,业主(买方 )通常采用... 详细信息

人类社会的商品交换从以物易物 ,经过以货币为媒介进行交易 ,到电子购物 ,商品交易范围日益扩大。今天 ,通过网络可以在全球范围实现在线即时交易。建筑产品作为大宗交易的特殊商品 ,它的购买方法不同于一般商品 ,业主(买方 )通常采用招标投标的竞争形式 ,进行“期货”交易。建筑市场也不同于一般的商品市场 ,有其特殊的法律环境、市场条件和管理系统 ,是独一无二的特殊交易场所。建筑产品的交易方式无疑反映了建筑市场的发展水平。目前 ,我国建筑市场基本上仍然沿用传统的项目建设模式 ,即设计—招标—建造方式。改革开放二十多年来 ,这一局面仍然未从根本上得以改变 ,成为深化建筑市场改革的主要障碍之一。建设部 2 0 0 0年在河北省、上海市和沈阳市进行我国建筑市场改革的试点 ,改革的内容之一是变革项目建设的组织模式 ,提倡工程总承包 ,有条件的要实施ＢＯＴ项目。用国际通用术语表示 ,就是要变革项目采购方式 ,改变我国建筑市场采购方式单一的局面 ,以此推动我国建筑业管理体制 ,管理机制和法制的协调发展 ,全面提升项目管理水平 ,以缓解加入ＷＴＯ对我国建筑业的压力 ,加速与国际惯例接轨。一、项目采购方式项目采购方式 (Ｐｒｏｊｅ...

关键词：建筑采购方式企业承包商企业管理建筑业体制中华人民共和国

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威士忌配绿茶，何不大胆一试？

商业周刊（中文版） 2011年第7期 8-9页

作者：克莱门汀·弗莱彻（Clementine fletcher）张晓（译）

当年老上海的法租界如今已变身时尚前沿阵地，在这里，帝亚吉欧公司（Diageo）的上海体验中心于6月起正式开幕。“尊邸”（Johnniew.lkerHouse）的设立以在中国推广尊尼获加（Johnniew.lker）品牌为主要目标。上海思南公馆的这座旧宅如... 详细信息

当年老上海的法租界如今已变身时尚前沿阵地，在这里，帝亚吉欧公司（Diageo）的上海体验中心于6月起正式开幕。“尊邸”（Johnniew.lkerHouse）的设立以在中国推广尊尼获加（Johnniew.lker）品牌为主要目标。上海思南公馆的这座旧宅如今价值已逾200万英镑（约合320万美元），设在这座公馆内的“尊邸”装潢别致，

关键词：威士忌绿茶法租界上海